UniProtKB - P18760 (COF1_MOUSE)
Protein
Cofilin-1
Gene
Cfl1
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11809832). Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity). Required for neural tube morphogenesis and neural crest cell migration (PubMed:15649475).By similarity2 Publications
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: UniProtKB
- phosphatidylinositol bisphosphate binding Source: MGI
- protein phosphatase binding Source: MGI
- signaling receptor binding Source: ARUK-UCL
GO - Biological processi
- actin filament depolymerization Source: UniProtKB
- actin filament fragmentation Source: UniProtKB
- actin filament organization Source: MGI
- actin filament severing Source: GO_Central
- cell motility Source: GO_Central
- cell projection organization Source: MGI
- cellular response to epidermal growth factor stimulus Source: MGI
- cellular response to ether Source: Ensembl
- cellular response to hydrogen peroxide Source: MGI
- cellular response to insulin-like growth factor stimulus Source: MGI
- cellular response to interleukin-1 Source: MGI
- cellular response to interleukin-6 Source: MGI
- cellular response to tumor necrosis factor Source: MGI
- cytoskeleton organization Source: UniProtKB
- establishment of cell polarity Source: MGI
- hippocampus development Source: Ensembl
- mitotic cytokinesis Source: MGI
- modification of postsynaptic actin cytoskeleton Source: MGI
- negative regulation of actin filament bundle assembly Source: MGI
- negative regulation of actin filament depolymerization Source: MGI
- negative regulation of cell adhesion Source: MGI
- negative regulation of cell motility Source: MGI
- negative regulation of cell size Source: MGI
- negative regulation of dendritic spine maintenance Source: MGI
- negative regulation of lamellipodium assembly Source: MGI
- negative regulation of postsynaptic density organization Source: MGI
- negative regulation of unidimensional cell growth Source: MGI
- neural crest cell migration Source: MGI
- neural fold formation Source: MGI
- positive regulation by host of viral process Source: MGI
- positive regulation of actin filament depolymerization Source: CACAO
- positive regulation of barbed-end actin filament capping Source: MGI
- positive regulation of cell growth Source: MGI
- positive regulation of cell motility Source: MGI
- positive regulation of dendritic spine development Source: MGI
- positive regulation of establishment of cell polarity regulating cell shape Source: MGI
- positive regulation of focal adhesion assembly Source: MGI
- positive regulation of lamellipodium assembly Source: MGI
- positive regulation of NMDA glutamate receptor activity Source: MGI
- positive regulation of protein localization to cell leading edge Source: MGI
- positive regulation of proteolysis Source: MGI
- positive regulation of synaptic plasticity Source: MGI
- protein import into nucleus Source: MGI
- protein phosphorylation Source: MGI
- regulation of cell morphogenesis Source: UniProtKB
- regulation of dendritic spine morphogenesis Source: ParkinsonsUK-UCL
- response to activity Source: Ensembl
- response to amino acid Source: MGI
- response to organic cyclic compound Source: Ensembl
- response to virus Source: Ensembl
Keywordsi
Molecular function | Actin-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cofilin-1Alternative name(s): Cofilin, non-muscle isoform |
Gene namesi | Name:Cfl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101757, Cfl1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Plasma membrane
- ruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- lamellipodium membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Nucleus
- Nucleus matrix By similarity
Other locations
- lamellipodium 1 Publication
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock (By similarity).By similarity
Cytoskeleton
- actin cytoskeleton Source: GO_Central
- cofilin-actin rod Source: MGI
- cortical actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular region Source: Reactome
Mitochondrion
- mitochondrial membrane Source: MGI
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
Plasma Membrane
- lamellipodium membrane Source: UniProtKB-SubCell
- ruffle membrane Source: UniProtKB-SubCell
- synaptic membrane Source: MGI
Other locations
- cell leading edge Source: MGI
- cell-cell junction Source: MGI
- cytoplasm Source: MGI
- dendritic spine Source: MGI
- filopodium Source: MGI
- focal adhesion Source: MGI
- glutamatergic synapse Source: MGI
- growth cone Source: MGI
- lamellipodium Source: UniProtKB
- neuron projection Source: MGI
- neuronal cell body Source: MGI
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000214900 | 2 – 166 | Cofilin-1Add BLAST | 165 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources2 Publications | 1 | |
Modified residuei | 3 | PhosphoserineCombined sources2 Publications | 1 | |
Modified residuei | 8 | PhosphoserineCombined sources | 1 | |
Modified residuei | 13 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 25 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 41 | PhosphoserineBy similarity | 1 | |
Modified residuei | 63 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 68 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 73 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 82 | Phosphotyrosine1 Publication | 1 | |
Cross-linki | 132 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 140 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 144 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 156 | PhosphoserineCombined sources1 Publication | 1 |
Post-translational modificationi
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (PubMed:25107909). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2 (By similarity).By similarity1 Publication
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3906 |
EPDi | P18760 |
jPOSTi | P18760 |
PaxDbi | P18760 |
PeptideAtlasi | P18760 |
PRIDEi | P18760 |
TopDownProteomicsi | P18760 |
2D gel databases
REPRODUCTION-2DPAGEi | P18760 |
SWISS-2DPAGEi | P18760 |
PTM databases
iPTMneti | P18760 |
PhosphoSitePlusi | P18760 |
SwissPalmi | P18760 |
Expressioni
Tissue specificityi
Widely distributed in various tissues. Not found in skeletal muscle.1 Publication
Developmental stagei
In 10.5 dpc embryo somites is expressed in a ventral cell layer (myotome).1 Publication
Gene expression databases
ExpressionAtlasi | P18760, baseline and differential |
Interactioni
Subunit structurei
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.
By similarityGO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: UniProtKB
- protein phosphatase binding Source: MGI
- signaling receptor binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 198684, 18 interactors |
DIPi | DIP-38073N |
IntActi | P18760, 13 interactors |
MINTi | P18760 |
STRINGi | 10090.ENSMUSP00000112259 |
Miscellaneous databases
RNActi | P18760, protein |
Structurei
3D structure databases
BMRBi | P18760 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 153 | ADF-HPROSITE-ProRule annotationAdd BLAST | 150 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 30 – 34 | Nuclear localization signalSequence analysis | 5 |
Sequence similaritiesi
Belongs to the actin-binding proteins ADF family.Curated
Phylogenomic databases
eggNOGi | KOG1735, Eukaryota |
GeneTreei | ENSGT00950000183000 |
InParanoidi | P18760 |
OMAi | MGVDENC |
OrthoDBi | 1370477at2759 |
PhylomeDBi | P18760 |
Family and domain databases
CDDi | cd11286, ADF_cofilin_like, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR017904, ADF/Cofilin IPR027234, Cofilin_1 |
PANTHERi | PTHR11913, PTHR11913, 1 hit PTHR11913:SF17, PTHR11913:SF17, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit |
PRINTSi | PR00006, COFILIN |
SMARTi | View protein in SMART SM00102, ADF, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P18760-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE
60 70 80 90 100
EGKEILVGDV GQTVDDPYTT FVKMLPDKDC RYALYDATYE TKESKKEDLV
110 120 130 140 150
FIFWAPENAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA
160
EKLGGSAVIS LEGKPL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WGL3 | F8WGL3_MOUSE | Cofilin, non-muscle isoform | Cfl1 | 227 | Annotation score: | ||
A0A494B9A7 | A0A494B9A7_MOUSE | Cofilin, non-muscle isoform | Cfl1 | 178 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00472 mRNA Translation: BAA00364.1 BC046225 mRNA Translation: AAH46225.1 BC058726 mRNA Translation: AAH58726.1 |
CCDSi | CCDS84409.1 |
PIRi | S12584 |
RefSeqi | NP_031713.1, NM_007687.5 |
Genome annotation databases
Ensembli | ENSMUST00000209469; ENSMUSP00000147514; ENSMUSG00000056201 |
GeneIDi | 12631 |
KEGGi | mmu:12631 |
UCSCi | uc008gdq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00472 mRNA Translation: BAA00364.1 BC046225 mRNA Translation: AAH46225.1 BC058726 mRNA Translation: AAH58726.1 |
CCDSi | CCDS84409.1 |
PIRi | S12584 |
RefSeqi | NP_031713.1, NM_007687.5 |
3D structure databases
BMRBi | P18760 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 198684, 18 interactors |
DIPi | DIP-38073N |
IntActi | P18760, 13 interactors |
MINTi | P18760 |
STRINGi | 10090.ENSMUSP00000112259 |
PTM databases
iPTMneti | P18760 |
PhosphoSitePlusi | P18760 |
SwissPalmi | P18760 |
2D gel databases
REPRODUCTION-2DPAGEi | P18760 |
SWISS-2DPAGEi | P18760 |
Proteomic databases
CPTACi | non-CPTAC-3906 |
EPDi | P18760 |
jPOSTi | P18760 |
PaxDbi | P18760 |
PeptideAtlasi | P18760 |
PRIDEi | P18760 |
TopDownProteomicsi | P18760 |
Protocols and materials databases
Antibodypediai | 16010, 1014 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000209469; ENSMUSP00000147514; ENSMUSG00000056201 |
GeneIDi | 12631 |
KEGGi | mmu:12631 |
UCSCi | uc008gdq.2, mouse |
Organism-specific databases
CTDi | 1072 |
MGIi | MGI:101757, Cfl1 |
Phylogenomic databases
eggNOGi | KOG1735, Eukaryota |
GeneTreei | ENSGT00950000183000 |
InParanoidi | P18760 |
OMAi | MGVDENC |
OrthoDBi | 1370477at2759 |
PhylomeDBi | P18760 |
Miscellaneous databases
BioGRID-ORCSi | 12631, 5 hits in 8 CRISPR screens |
ChiTaRSi | Cfl1, mouse |
PROi | PR:P18760 |
RNActi | P18760, protein |
SOURCEi | Search... |
Gene expression databases
ExpressionAtlasi | P18760, baseline and differential |
Family and domain databases
CDDi | cd11286, ADF_cofilin_like, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR017904, ADF/Cofilin IPR027234, Cofilin_1 |
PANTHERi | PTHR11913, PTHR11913, 1 hit PTHR11913:SF17, PTHR11913:SF17, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit |
PRINTSi | PR00006, COFILIN |
SMARTi | View protein in SMART SM00102, ADF, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COF1_MOUSE | |
Accessioni | P18760Primary (citable) accession number: P18760 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 203 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families