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Protein

Regulator of chromosome condensation

Gene

RCC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).8 Publications

Miscellaneous

Patients with Raynaud disease produce antibodies that bind to RCC1.

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • histone binding Source: UniProtKB
  • nucleosomal DNA binding Source: UniProtKB
  • nucleosome binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • Ran GTPase binding Source: CAFA
  • Ran guanyl-nucleotide exchange factor activity Source: UniProtKB
  • sulfate binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Guanine-nucleotide releasing factor
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-180746 Nuclear import of Rev protein

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of chromosome condensation
Alternative name(s):
Cell cycle regulatory protein
Chromosome condensation protein 1
Gene namesi
Name:RCC1
Synonyms:CHC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000180198.15
HGNCiHGNC:1913 RCC1
MIMi179710 gene
neXtProtiNX_P18754

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2S → A: Does not abolish N-terminal methylation. 1 Publication1
Mutagenesisi2S → Q: Does not abolish N-terminal methylation. 1 Publication1
Mutagenesisi3P → Q: Abolishes N-terminal methylation. 1 Publication1
Mutagenesisi4K → Q: Abolishes N-terminal methylation. 2 Publications1
Mutagenesisi4K → R: Stongly impairs N-terminal methylation and subcellular localization. 2 Publications1
Mutagenesisi9R → A: Decreases KPNA4 binding. Strongly decreases KPNA4 binding; when associated with A-21. 1 Publication1
Mutagenesisi11S → E: Phosphomimetic mutant. Decreases KPNA4 binding by about 10%. 1 Publication1
Mutagenesisi21K → A: Decreases KPNA4 binding. Strongly decreases KPNA4 binding; when associated with A-9. 1 Publication1
Mutagenesisi182D → A: Abolishes interaction with Ran and impairs chromosome localization. 2 Publications1

Organism-specific databases

DisGeNETi1104
OpenTargetsiENSG00000180198
PharmGKBiPA26449

Polymorphism and mutation databases

BioMutaiRCC1
DMDMi132170

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00002066282 – 421Regulator of chromosome condensationAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylserine; alternate3 Publications1
Modified residuei2N,N-dimethylserine; alternate3 Publications1
Modified residuei2N-methylserine; alternate3 Publications1
Modified residuei2Phosphoserine1 Publication1
Modified residuei11PhosphoserineCombined sources1

Post-translational modificationi

N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.3 Publications

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP18754
PeptideAtlasiP18754
PRIDEiP18754
ProteomicsDBi53606
53607 [P18754-2]
TopDownProteomicsiP18754-1 [P18754-1]

PTM databases

iPTMnetiP18754
PhosphoSitePlusiP18754
SwissPalmiP18754

Expressioni

Gene expression databases

BgeeiENSG00000180198 Expressed in 204 organ(s), highest expression level in testis
CleanExiHS_RCC1
ExpressionAtlasiP18754 baseline and differential
GenevisibleiP18754 HS

Organism-specific databases

HPAiCAB015413
HPA027573
HPA027574

Interactioni

Subunit structurei

Interacts with RAN (PubMed:17435751, PubMed:18762580, PubMed:29040603, PubMed:11336674). Interacts (via N-terminus and RCC1 repeats) with KPNA4 (PubMed:29042532). Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation (PubMed:16820410).6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107529, 105 interactors
DIPiDIP-35416N
IntActiP18754, 63 interactors
MINTiP18754

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP18754
SMRiP18754
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18754

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati34 – 84RCC1 1Add BLAST51
Repeati85 – 136RCC1 2Add BLAST52
Repeati138 – 189RCC1 3Add BLAST52
Repeati191 – 257RCC1 4Add BLAST67
Repeati258 – 311RCC1 5Add BLAST54
Repeati312 – 362RCC1 6Add BLAST51
Repeati363 – 416RCC1 7Add BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi4 – 24Bipartite nuclear localization signal1 PublicationAdd BLAST21

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00910000144029
HOGENOMiHOG000234341
HOVERGENiHBG017712
InParanoidiP18754
KOiK11493
OMAiEGTVCCS
OrthoDBiEOG091G05E6
PhylomeDBiP18754
TreeFamiTF101139

Family and domain databases

Gene3Di2.130.10.30, 1 hit
InterProiView protein in InterPro
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
PfamiView protein in Pfam
PF00415 RCC1, 7 hits
PRINTSiPR00633 RCCNDNSATION
SUPFAMiSSF50985 SSF50985, 1 hit
PROSITEiView protein in PROSITE
PS00625 RCC1_1, 1 hit
PS00626 RCC1_2, 4 hits
PS50012 RCC1_3, 7 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P18754-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSPKRIAKRR SPPADAIPKS KKVKVSHRSH STEPGLVLTL GQGDVGQLGL
60 70 80 90 100
GENVMERKKP ALVSIPEDVV QAEAGGMHTV CLSKSGQVYS FGCNDEGALG
110 120 130 140 150
RDTSVEGSEM VPGKVELQEK VVQVSAGDSH TAALTDDGRV FLWGSFRDNN
160 170 180 190 200
GVIGLLEPMK KSMVPVQVQL DVPVVKVASG NDHLVMLTAD GDLYTLGCGE
210 220 230 240 250
QGQLGRVPEL FANRGGRQGL ERLLVPKCVM LKSRGSRGHV RFQDAFCGAY
260 270 280 290 300
FTFAISHEGH VYGFGLSNYH QLGTPGTESC FIPQNLTSFK NSTKSWVGFS
310 320 330 340 350
GGQHHTVCMD SEGKAYSLGR AEYGRLGLGE GAEEKSIPTL ISRLPAVSSV
360 370 380 390 400
ACGASVGYAV TKDGRVFAWG MGTNYQLGTG QDEDAWSPVE MMGKQLENRV
410 420
VLSVSSGGQH TVLLVKDKEQ S
Length:421
Mass (Da):44,969
Last modified:November 1, 1990 - v1
Checksum:iF6D225AF81928305
GO
Isoform 2 (identifier: P18754-2) [UniParc]FASTAAdd to basket
Also known as: RCC1-I

The sequence of this isoform differs from the canonical sequence as follows:
     24-24: K → KDTRAAASRRVPGARSCQGACGPSPPDQKTRP

Show »
Length:452
Mass (Da):48,146
Checksum:iA31255E99A01A25F
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JW69C9JW69_HUMAN
Regulator of chromosome condensatio...
RCC1
372Annotation score:
C9JRH2C9JRH2_HUMAN
Regulator of chromosome condensatio...
RCC1
230Annotation score:
C9JQZ4C9JQZ4_HUMAN
Regulator of chromosome condensatio...
RCC1
283Annotation score:
C9JMJ4C9JMJ4_HUMAN
Regulator of chromosome condensatio...
RCC1
272Annotation score:
C9J3R0C9J3R0_HUMAN
Regulator of chromosome condensatio...
RCC1
272Annotation score:
M0R1Q8M0R1Q8_HUMAN
Regulator of chromosome condensatio...
RCC1
148Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04112224K → KDTRAAASRRVPGARSCQGA CGPSPPDQKTRP in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12654 mRNA Translation: CAA31182.1
X06130 mRNA Translation: CAA29496.1
D00591 Genomic DNA Translation: BAA00469.1
AF498924 mRNA Translation: AAM21072.1
BC007300 mRNA Translation: AAH07300.1
BC010067 mRNA Translation: AAH10067.1
BC036903 mRNA Translation: AAH36903.1
BC069198 mRNA Translation: AAH69198.1
S75708 mRNA Translation: AAB32653.1
CCDSiCCDS323.1 [P18754-1]
CCDS41295.1 [P18754-2]
PIRiA26691
RefSeqiNP_001041659.1, NM_001048194.2 [P18754-2]
NP_001041660.1, NM_001048195.2
NP_001041664.1, NM_001048199.2 [P18754-1]
NP_001260.1, NM_001269.4 [P18754-1]
UniGeneiHs.469723

Genome annotation databases

EnsembliENST00000373831; ENSP00000362937; ENSG00000180198 [P18754-2]
ENST00000373832; ENSP00000362938; ENSG00000180198 [P18754-1]
ENST00000373833; ENSP00000362939; ENSG00000180198 [P18754-1]
ENST00000398958; ENSP00000381931; ENSG00000180198 [P18754-1]
GeneIDi1104
KEGGihsa:1104
UCSCiuc001bqf.3 human [P18754-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12654 mRNA Translation: CAA31182.1
X06130 mRNA Translation: CAA29496.1
D00591 Genomic DNA Translation: BAA00469.1
AF498924 mRNA Translation: AAM21072.1
BC007300 mRNA Translation: AAH07300.1
BC010067 mRNA Translation: AAH10067.1
BC036903 mRNA Translation: AAH36903.1
BC069198 mRNA Translation: AAH69198.1
S75708 mRNA Translation: AAB32653.1
CCDSiCCDS323.1 [P18754-1]
CCDS41295.1 [P18754-2]
PIRiA26691
RefSeqiNP_001041659.1, NM_001048194.2 [P18754-2]
NP_001041660.1, NM_001048195.2
NP_001041664.1, NM_001048199.2 [P18754-1]
NP_001260.1, NM_001269.4 [P18754-1]
UniGeneiHs.469723

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A12X-ray1.70A/B/C9-421[»]
1I2MX-ray1.76B/D20-421[»]
5E1BX-ray1.65D/E2-7[»]
5E1DX-ray1.45D/E3-7[»]
5E1MX-ray1.75D/E3-7[»]
5E1OX-ray2.00D/E3-7[»]
5E2AX-ray1.75D/E3-7[»]
5E2BX-ray1.95D/E3-7[»]
5TBKX-ray3.45I/J/K/L/M/N/O/P1-421[»]
ProteinModelPortaliP18754
SMRiP18754
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107529, 105 interactors
DIPiDIP-35416N
IntActiP18754, 63 interactors
MINTiP18754

PTM databases

iPTMnetiP18754
PhosphoSitePlusiP18754
SwissPalmiP18754

Polymorphism and mutation databases

BioMutaiRCC1
DMDMi132170

Proteomic databases

EPDiP18754
PeptideAtlasiP18754
PRIDEiP18754
ProteomicsDBi53606
53607 [P18754-2]
TopDownProteomicsiP18754-1 [P18754-1]

Protocols and materials databases

DNASUi1104
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373831; ENSP00000362937; ENSG00000180198 [P18754-2]
ENST00000373832; ENSP00000362938; ENSG00000180198 [P18754-1]
ENST00000373833; ENSP00000362939; ENSG00000180198 [P18754-1]
ENST00000398958; ENSP00000381931; ENSG00000180198 [P18754-1]
GeneIDi1104
KEGGihsa:1104
UCSCiuc001bqf.3 human [P18754-1]

Organism-specific databases

CTDi1104
DisGeNETi1104
EuPathDBiHostDB:ENSG00000180198.15
GeneCardsiRCC1
HGNCiHGNC:1913 RCC1
HPAiCAB015413
HPA027573
HPA027574
MIMi179710 gene
neXtProtiNX_P18754
OpenTargetsiENSG00000180198
PharmGKBiPA26449
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00910000144029
HOGENOMiHOG000234341
HOVERGENiHBG017712
InParanoidiP18754
KOiK11493
OMAiEGTVCCS
OrthoDBiEOG091G05E6
PhylomeDBiP18754
TreeFamiTF101139

Enzyme and pathway databases

ReactomeiR-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-180746 Nuclear import of Rev protein

Miscellaneous databases

ChiTaRSiRCC1 human
EvolutionaryTraceiP18754
GeneWikiiRCC1
GenomeRNAii1104
PROiPR:P18754
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000180198 Expressed in 204 organ(s), highest expression level in testis
CleanExiHS_RCC1
ExpressionAtlasiP18754 baseline and differential
GenevisibleiP18754 HS

Family and domain databases

Gene3Di2.130.10.30, 1 hit
InterProiView protein in InterPro
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
PfamiView protein in Pfam
PF00415 RCC1, 7 hits
PRINTSiPR00633 RCCNDNSATION
SUPFAMiSSF50985 SSF50985, 1 hit
PROSITEiView protein in PROSITE
PS00625 RCC1_1, 1 hit
PS00626 RCC1_2, 4 hits
PS50012 RCC1_3, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRCC1_HUMAN
AccessioniPrimary (citable) accession number: P18754
Secondary accession number(s): Q16269, Q6NT97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 7, 2018
This is version 200 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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