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Entry version 176 (16 Oct 2019)
Sequence version 3 (08 Feb 2011)
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Protein

DNA-directed RNA polymerase II subunit 1

Gene

NRPB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from NRPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (NRPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.1 Publication

Miscellaneous

The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi66Zinc 1By similarity1
Metal bindingi69Zinc 1By similarity1
Metal bindingi76Zinc 1By similarity1
Metal bindingi79Zinc 1; via tele nitrogenBy similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi109Zinc 2By similarity1
Metal bindingi147Zinc 2By similarity1
Metal bindingi172Zinc 2By similarity1
Metal bindingi495Magnesium; catalyticBy similarity1
Metal bindingi497Magnesium; catalyticBy similarity1
Metal bindingi499Magnesium; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi326 – 397By similarityAdd BLAST72

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processTranscription
LigandMagnesium, Metal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit 1
Alternative name(s):
DNA-directed RNA polymerase II subunit RPB1 (EC:2.7.7.6)
Short name:
DNA polymerase II subunit B1
DNA-directed RNA polymerase III largest subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NRPB1
Synonyms:RPB1, RPB205, RPII
Ordered Locus Names:At4g35800
ORF Names:F4B14.70
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT4G35800

The Arabidopsis Information Resource

More...
TAIRi
locus:2125319 AT4G35800

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000739331 – 1839DNA-directed RNA polymerase II subunit 1Add BLAST1839

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatase, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P18616

PRoteomics IDEntifications database

More...
PRIDEi
P18616

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P18616

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
P18616 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
P18616 AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of at least 12 subunits.

Interacts with RDM1.

Interacts (via CTD) with MED35A, MED35B, MED35C and CYP59 (PubMed:16497658, PubMed:19110459, PubMed:19467629, PubMed:20410883).

Interacts with MEE12/CCG1 and MEE14/CBP1 (PubMed:26462908).

5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
15016, 14 interactors

Database of interacting proteins

More...
DIPi
DIP-40008N

Protein interaction database and analysis system

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IntActi
P18616, 4 interactors

Molecular INTeraction database

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MINTi
P18616

STRING: functional protein association networks

More...
STRINGi
3702.AT4G35800.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P18616

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati1544 – 155017
Repeati1551 – 155727
Repeati1558 – 156437
Repeati1565 – 157147
Repeati1572 – 157857
Repeati1579 – 158567
Repeati1586 – 159277
Repeati1593 – 159987
Repeati1600 – 160697
Repeati1607 – 1613107
Repeati1614 – 1620117
Repeati1621 – 1627127
Repeati1628 – 1634137
Repeati1635 – 1641147
Repeati1642 – 1648157
Repeati1649 – 1655167
Repeati1656 – 1662177
Repeati1663 – 1669187
Repeati1670 – 1676197
Repeati1677 – 1683207
Repeati1684 – 1690217
Repeati1691 – 1697227
Repeati1698 – 1704237
Repeati1705 – 1711247
Repeati1712 – 1718257
Repeati1719 – 1725267
Repeati1726 – 1732277
Repeati1733 – 173828; approximate6
Repeati1739 – 1745297
Repeati1752 – 1758307
Repeati1759 – 1765317
Repeati1766 – 1772327
Repeati1773 – 1779337
Repeati1780 – 1786347
Repeati1794 – 179935; approximate6
Repeati1800 – 1806367
Repeati1807 – 1813377

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni785 – 795Alpha-amanitin bindingAdd BLAST11
Regioni829 – 841Bridging helixAdd BLAST13
Regioni1544 – 1813C-terminal domain (CTD); 37 X 7 AA tandem approximate repeats of Y-[GNS]-P-[QST]-[LNS]-[APT]-[AGKNRSTY]Add BLAST270

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The C-terminal domain (CTD) serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.Curated

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG0260 Eukaryota
COG0086 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000222975

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P18616

KEGG Orthology (KO)

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KOi
K03006

Identification of Orthologs from Complete Genome Data

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OMAi
KSAPCMG

Database of Orthologous Groups

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OrthoDBi
591636at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P18616

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.132.30, 1 hit
1.10.274.100, 1 hit
3.30.1360.140, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000722 RNA_pol_asu
IPR000684 RNA_pol_II_repeat_euk
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR042102 RNA_pol_Rpb1_3_sf
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR007075 RNA_pol_Rpb1_6
IPR007073 RNA_pol_Rpb1_7
IPR038593 RNA_pol_Rpb1_7_sf
IPR038120 Rpb1_funnel_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
PF04992 RNA_pol_Rpb1_6, 1 hit
PF04990 RNA_pol_Rpb1_7, 1 hit
PF05001 RNA_pol_Rpb1_R, 15 hits

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00663 RPOLA_N, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00115 RNA_POL_II_REPEAT, 23 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P18616-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDTRFPFSPA EVSKVRVVQF GILSPDEIRQ MSVIHVEHSE TTEKGKPKVG
60 70 80 90 100
GLSDTRLGTI DRKVKCETCM ANMAECPGHF GYLELAKPMY HVGFMKTVLS
110 120 130 140 150
IMRCVCFNCS KILADEEEHK FKQAMKIKNP KNRLKKILDA CKNKTKCDGG
160 170 180 190 200
DDIDDVQSHS TDEPVKKSRG GCGAQQPKLT IEGMKMIAEY KIQRKKNDEP
210 220 230 240 250
DQLPEPAERK QTLGADRVLS VLKRISDADC QLLGFNPKFA RPDWMILEVL
260 270 280 290 300
PIPPPPVRPS VMMDATSRSE DDLTHQLAMI IRHNENLKRQ EKNGAPAHII
310 320 330 340 350
SEFTQLLQFH IATYFDNELP GQPRATQKSG RPIKSICSRL KAKEGRIRGN
360 370 380 390 400
LMGKRVDFSA RTVITPDPTI NIDELGVPWS IALNLTYPET VTPYNIERLK
410 420 430 440 450
ELVDYGPHPP PGKTGAKYII RDDGQRLDLR YLKKSSDQHL ELGYKVERHL
460 470 480 490 500
QDGDFVLFNR QPSLHKMSIM GHRIRIMPYS TFRLNLSVTS PYNADFDGDE
510 520 530 540 550
MNMHVPQSFE TRAEVLELMM VPKCIVSPQA NRPVMGIVQD TLLGCRKITK
560 570 580 590 600
RDTFIEKDVF MNTLMWWEDF DGKVPAPAIL KPRPLWTGKQ VFNLIIPKQI
610 620 630 640 650
NLLRYSAWHA DTETGFITPG DTQVRIERGE LLAGTLCKKT LGTSNGSLVH
660 670 680 690 700
VIWEEVGPDA ARKFLGHTQW LVNYWLLQNG FTIGIGDTIA DSSTMEKINE
710 720 730 740 750
TISNAKTAVK DLIRQFQGKE LDPEPGRTMR DTFENRVNQV LNKARDDAGS
760 770 780 790 800
SAQKSLAETN NLKAMVTAGS KGSFINISQM TACVGQQNVE GKRIPFGFDG
810 820 830 840 850
RTLPHFTKDD YGPESRGFVE NSYLRGLTPQ EFFFHAMGGR EGLIDTAVKT
860 870 880 890 900
SETGYIQRRL VKAMEDIMVK YDGTVRNSLG DVIQFLYGED GMDAVWIESQ
910 920 930 940 950
KLDSLKMKKS EFDRTFKYEI DDENWNPTYL SDEHLEDLKG IRELRDVFDA
960 970 980 990 1000
EYSKLETDRF QLGTEIATNG DSTWPLPVNI KRHIWNAQKT FKIDLRKISD
1010 1020 1030 1040 1050
MHPVEIVDAV DKLQERLLVV PGDDALSVEA QKNATLFFNI LLRSTLASKR
1060 1070 1080 1090 1100
VLEEYKLSRE AFEWVIGEIE SRFLQSLVAP GEMIGCVAAQ SIGEPATQMT
1110 1120 1130 1140 1150
LNTFHYAGVS AKNVTLGVPR LREIINVAKR IKTPSLSVYL TPEASKSKEG
1160 1170 1180 1190 1200
AKTVQCALEY TTLRSVTQAT EVWYDPDPMS TIIEEDFEFV RSYYEMPDED
1210 1220 1230 1240 1250
VSPDKISPWL LRIELNREMM VDKKLSMADI AEKINLEFDD DLTCIFNDDN
1260 1270 1280 1290 1300
AQKLILRIRI MNDEGPKGEL QDESAEDDVF LKKIESNMLT EMALRGIPDI
1310 1320 1330 1340 1350
NKVFIKQVRK SRFDEEGGFK TSEEWMLDTE GVNLLAVMCH EDVDPKRTTS
1360 1370 1380 1390 1400
NHLIEIIEVL GIEAVRRALL DELRVVISFD GSYVNYRHLA ILCDTMTYRG
1410 1420 1430 1440 1450
HLMAITRHGI NRNDTGPLMR CSFEETVDIL LDAAAYAETD CLRGVTENIM
1460 1470 1480 1490 1500
LGQLAPIGTG DCELYLNDEM LKNAIELQLP SYMDGLEFGM TPARSPVSGT
1510 1520 1530 1540 1550
PYHEGMMSPN YLLSPNMRLS PMSDAQFSPY VGGMAFSPSS SPGYSPSSPG
1560 1570 1580 1590 1600
YSPTSPGYSP TSPGYSPTSP GYSPTSPTYS PSSPGYSPTS PAYSPTSPSY
1610 1620 1630 1640 1650
SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP AYSPTSPAYS
1660 1670 1680 1690 1700
PTSPAYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPAYSP
1710 1720 1730 1740 1750
TSPGYSPTSP SYSPTSPSYG PTSPSYNPQS AKYSPSIAYS PSNARLSPAS
1760 1770 1780 1790 1800
PYSPTSPNYS PTSPSYSPTS PSYSPSSPTY SPSSPYSSGA SPDYSPSAGY
1810 1820 1830
SPTLPGYSPS STGQYTPHEG DKKDKTGKKD ASKDDKGNP
Length:1,839
Mass (Da):204,626
Last modified:February 8, 2011 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7B8488DBB5180B40
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA36735 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti117 – 122EEHKFK → VCRSLFR in CAA37130 (PubMed:2259344).Curated6
Sequence conflicti192 – 196IQRKK → NSKEE in CAA36735 (PubMed:2103447).Curated5
Sequence conflicti297A → R in CAA36735 (PubMed:2103447).Curated1
Sequence conflicti302E → R in CAA36735 (PubMed:2103447).Curated1
Sequence conflicti400 – 416KELVD…GKTGA → VRLVFISFSET in CAA37130 (PubMed:2259344).CuratedAdd BLAST17
Sequence conflicti427L → S in CAA37130 (PubMed:2259344).Curated1
Sequence conflicti738N → D in CAA37130 (PubMed:2259344).Curated1
Sequence conflicti1061A → R in CAA37130 (PubMed:2259344).Curated1
Sequence conflicti1088A → P in CAA36735 (PubMed:2103447).Curated1
Sequence conflicti1719Y → YSPTSPSY in CAA37130 (PubMed:2259344).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
X52954 Genomic DNA Translation: CAA37130.1
X52494 Genomic DNA Translation: CAA36735.1 Sequence problems.
AL031986 Genomic DNA Translation: CAA21466.2
AL161588 Genomic DNA Translation: CAB81489.1
CP002687 Genomic DNA Translation: AEE86573.1
CP002687 Genomic DNA Translation: ANM67520.1
AK221166 mRNA Translation: BAD95215.1

Protein sequence database of the Protein Information Resource

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PIRi
G85422
T04690 JDMU1

NCBI Reference Sequences

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RefSeqi
NP_001329346.1, NM_001342374.1
NP_195305.2, NM_119746.4

Genome annotation databases

Ensembl plant genome annotation project

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EnsemblPlantsi
AT4G35800.1; AT4G35800.1; AT4G35800
AT4G35800.2; AT4G35800.2; AT4G35800

Database of genes from NCBI RefSeq genomes

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GeneIDi
829734

Gramene; a comparative resource for plants

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Gramenei
AT4G35800.1; AT4G35800.1; AT4G35800
AT4G35800.2; AT4G35800.2; AT4G35800

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
ath:AT4G35800

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52954 Genomic DNA Translation: CAA37130.1
X52494 Genomic DNA Translation: CAA36735.1 Sequence problems.
AL031986 Genomic DNA Translation: CAA21466.2
AL161588 Genomic DNA Translation: CAB81489.1
CP002687 Genomic DNA Translation: AEE86573.1
CP002687 Genomic DNA Translation: ANM67520.1
AK221166 mRNA Translation: BAD95215.1
PIRiG85422
T04690 JDMU1
RefSeqiNP_001329346.1, NM_001342374.1
NP_195305.2, NM_119746.4

3D structure databases

SMRiP18616
ModBaseiSearch...

Protein-protein interaction databases

BioGridi15016, 14 interactors
DIPiDIP-40008N
IntActiP18616, 4 interactors
MINTiP18616
STRINGi3702.AT4G35800.1

PTM databases

iPTMnetiP18616

Proteomic databases

PaxDbiP18616
PRIDEiP18616

Genome annotation databases

EnsemblPlantsiAT4G35800.1; AT4G35800.1; AT4G35800
AT4G35800.2; AT4G35800.2; AT4G35800
GeneIDi829734
GrameneiAT4G35800.1; AT4G35800.1; AT4G35800
AT4G35800.2; AT4G35800.2; AT4G35800
KEGGiath:AT4G35800

Organism-specific databases

AraportiAT4G35800
TAIRilocus:2125319 AT4G35800

Phylogenomic databases

eggNOGiKOG0260 Eukaryota
COG0086 LUCA
HOGENOMiHOG000222975
InParanoidiP18616
KOiK03006
OMAiKSAPCMG
OrthoDBi591636at2759
PhylomeDBiP18616

Miscellaneous databases

Protein Ontology

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PROi
PR:P18616

Gene expression databases

ExpressionAtlasiP18616 baseline and differential
GenevisibleiP18616 AT

Family and domain databases

Gene3Di1.10.132.30, 1 hit
1.10.274.100, 1 hit
3.30.1360.140, 1 hit
InterProiView protein in InterPro
IPR000722 RNA_pol_asu
IPR000684 RNA_pol_II_repeat_euk
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR042102 RNA_pol_Rpb1_3_sf
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR007075 RNA_pol_Rpb1_6
IPR007073 RNA_pol_Rpb1_7
IPR038593 RNA_pol_Rpb1_7_sf
IPR038120 Rpb1_funnel_sf
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
PF04992 RNA_pol_Rpb1_6, 1 hit
PF04990 RNA_pol_Rpb1_7, 1 hit
PF05001 RNA_pol_Rpb1_R, 15 hits
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit
PROSITEiView protein in PROSITE
PS00115 RNA_POL_II_REPEAT, 23 hits

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNRPB1_ARATH
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P18616
Secondary accession number(s): P31635, Q56Z04, Q9SZS8
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 8, 2011
Last modified: October 16, 2019
This is version 176 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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