UniProtKB - P18615 (NELFE_HUMAN)
Protein
Negative elongation factor E
Gene
NELFE
Organism
Homo sapiens (Human)
Status
Functioni
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27282391).5 Publications
(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.1 Publication
GO - Molecular functioni
- chromatin binding Source: Ensembl
- RNA binding Source: UniProtKB
GO - Biological processi
- negative regulation of mRNA polyadenylation Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- negative regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
- positive regulation of ERK1 and ERK2 cascade Source: Ensembl
- positive regulation of histone H3-K4 methylation Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of viral transcription Source: Reactome
- transcription by RNA polymerase II Source: Reactome
- transcription elongation from RNA polymerase II promoter Source: Reactome
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P18615 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-113418, Formation of the Early Elongation Complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167158, Formation of the HIV-1 Early Elongation Complex R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Negative elongation factor EShort name: NELF-E Alternative name(s): RNA-binding protein RD |
Gene namesi | Name:NELFE Synonyms:RD, RDBP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13974, NELFE |
MIMi | 154040, gene |
neXtProti | NX_P18615 |
VEuPathDBi | HostDB:ENSG00000204356.11 |
Subcellular locationi
Nucleus
Nucleus
- NELF complex Source: UniProtKB
- nuclear body Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 295 – 299 | RNCAF → EQMAT: Abolishes interaction with RNA but not the interaction with other proteins of the NELF complex. 1 Publication | 5 |
Organism-specific databases
DisGeNETi | 7936 |
OpenTargetsi | ENSG00000204356 |
PharmGKBi | PA134974984 |
Miscellaneous databases
Pharosi | P18615, Tbio |
Genetic variation databases
BioMutai | NELFE |
DMDMi | 1350554 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081802 | 1 – 380 | Negative elongation factor EAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | PhosphoserineCombined sources | 1 | |
Cross-linki | 78 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources | ||
Cross-linki | 78 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Cross-linki | 82 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 113 | PhosphoserineCombined sources | 1 | |
Modified residuei | 115 | PhosphoserineCombined sources | 1 | |
Modified residuei | 131 | PhosphoserineCombined sources | 1 | |
Modified residuei | 139 | PhosphoserineCombined sources | 1 | |
Modified residuei | 165 | PhosphoserineCombined sources | 1 | |
Modified residuei | 179 | PhosphoserineCombined sources | 1 | |
Modified residuei | 181 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineCombined sources | 1 | |
Modified residuei | 249 | PhosphoserineCombined sources | 1 | |
Modified residuei | 251 | PhosphoserineCombined sources | 1 | |
Modified residuei | 272 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 274 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 281 | PhosphoserineCombined sources | 1 | |
Modified residuei | 353 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Sumoylated.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-999 |
EPDi | P18615 |
jPOSTi | P18615 |
MassIVEi | P18615 |
MaxQBi | P18615 |
PaxDbi | P18615 |
PeptideAtlasi | P18615 |
PRIDEi | P18615 |
ProteomicsDBi | 5199 53602 [P18615-1] 5554 |
PTM databases
iPTMneti | P18615 |
MetOSitei | P18615 |
PhosphoSitePlusi | P18615 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000204356, Expressed in testis and 237 other tissues |
ExpressionAtlasi | P18615, baseline and differential |
Genevisiblei | P18615, HS |
Organism-specific databases
HPAi | ENSG00000204356, Low tissue specificity |
Interactioni
Subunit structurei
(Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
1 PublicationBinary interactionsi
Hide detailsP18615
Protein-protein interaction databases
BioGRIDi | 113662, 45 interactors |
CORUMi | P18615 |
DIPi | DIP-32669N |
IntActi | P18615, 30 interactors |
STRINGi | 9606.ENSP00000364578 |
Miscellaneous databases
RNActi | P18615, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P18615 |
SMRi | P18615 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P18615 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 184 – 185 | 1 | 2 | |
Repeati | 186 – 187 | 2 | 2 | |
Repeati | 188 – 189 | 3 | 2 | |
Repeati | 190 – 191 | 4 | 2 | |
Repeati | 192 – 193 | 5; approximate | 2 | |
Repeati | 194 – 195 | 6 | 2 | |
Repeati | 196 – 197 | 7 | 2 | |
Repeati | 198 – 199 | 8 | 2 | |
Repeati | 200 – 201 | 9 | 2 | |
Repeati | 202 – 203 | 10 | 2 | |
Repeati | 204 – 205 | 11 | 2 | |
Repeati | 206 – 207 | 12 | 2 | |
Repeati | 208 – 209 | 13 | 2 | |
Repeati | 210 – 211 | 14 | 2 | |
Repeati | 212 – 213 | 15 | 2 | |
Repeati | 214 – 215 | 16 | 2 | |
Repeati | 216 – 217 | 17 | 2 | |
Repeati | 218 – 219 | 18 | 2 | |
Repeati | 220 – 221 | 19 | 2 | |
Repeati | 222 – 223 | 20 | 2 | |
Repeati | 224 – 225 | 21 | 2 | |
Repeati | 226 – 227 | 22 | 2 | |
Repeati | 228 – 229 | 23 | 2 | |
Repeati | 230 – 231 | 24 | 2 | |
Repeati | 232 – 233 | 25 | 2 | |
Repeati | 234 – 235 | 26 | 2 | |
Repeati | 236 – 237 | 27 | 2 | |
Repeati | 238 – 239 | 28 | 2 | |
Repeati | 240 – 241 | 29 | 2 | |
Repeati | 242 – 243 | 30 | 2 | |
Domaini | 262 – 332 | RRMPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 184 – 243 | 30 X 2 AA approximate tandem repeats of R-[DSNE]Add BLAST | 60 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 7 – 36 | Sequence analysisAdd BLAST | 30 |
Domaini
The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.1 Publication
Sequence similaritiesi
Belongs to the RRM NELF-E family.Curated
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | ENOG502QQQ4, Eukaryota |
GeneTreei | ENSGT00630000089917 |
HOGENOMi | CLU_055643_0_0_1 |
InParanoidi | P18615 |
OMAi | RRQPIIE |
PhylomeDBi | P18615 |
TreeFami | TF324087 |
Family and domain databases
CDDi | cd12305, RRM_NELFE, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
IDEALi | IID00030 |
InterProi | View protein in InterPro IPR033102, NELFE IPR034637, NELFE_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR17250, PTHR17250, 2 hits |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P18615-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL
60 70 80 90 100
SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE
110 120 130 140 150
KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG
160 170 180 190 200
EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR
210 220 230 240 250
DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD
260 270 280 290 300
SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV
310 320 330 340 350
TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV
360 370 380
QNSPKGCHRD KRTQIVYSDD VYKENLVDGF
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MSN9 | A0A0A0MSN9_HUMAN | Negative elongation factor E | NELFE | 333 | Annotation score: | ||
A2ABK4 | A2ABK4_HUMAN | Negative elongation factor E | NELFE | 159 | Annotation score: | ||
E9PD43 | E9PD43_HUMAN | Negative elongation factor E | NELFE | 260 | Annotation score: | ||
A0A0A0MT02 | A0A0A0MT02_HUMAN | Negative elongation factor E | NELFE | 293 | Annotation score: | ||
A0A0G2JI50 | A0A0G2JI50_HUMAN | Negative elongation factor E | NELFE | 240 | Annotation score: | ||
E7ENC7 | E7ENC7_HUMAN | Negative elongation factor E | NELFE | 36 | Annotation score: | ||
E7EWR7 | E7EWR7_HUMAN | Negative elongation factor E | NELFE | 57 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 | D → DD in AAA36308 (PubMed:2612324).Curated | 1 | |
Sequence conflicti | 174 | S → M in CAA34231 (PubMed:2119325).Curated | 1 | |
Sequence conflicti | 265 | Y → YD in AAA36308 (PubMed:2612324).Curated | 1 | |
Sequence conflicti | 312 | V → A in AAA36308 (PubMed:2612324).Curated | 1 | |
Sequence conflicti | 327 | V → D in AAA36308 (PubMed:2612324).Curated | 1 | |
Sequence conflicti | 347 | S → A in AAA36308 (PubMed:2612324).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056151 | 1 | M → MVPKGATM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_056152 | 135 – 164 | Missing in isoform 3. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33231 , M32274, M32275, M32276, M33230 Genomic DNA Translation: AAA36308.1 L03411 mRNA Translation: AAC37523.1 AF019413 Genomic DNA Translation: AAB67979.1 AK300717 mRNA Translation: BAG62393.1 AK302652 mRNA Translation: BAG63891.1 AL049547 Genomic DNA Translation: CAB89308.1 AL662849 Genomic DNA No translation available. AL844853 Genomic DNA No translation available. BX005143 Genomic DNA No translation available. CR759782 Genomic DNA No translation available. CR388219 Genomic DNA No translation available. AL645922 Genomic DNA No translation available. CR753845 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03553.1 BC025235 mRNA Translation: AAH25235.1 BC050617 mRNA Translation: AAH50617.1 X16105 mRNA Translation: CAA34231.1 |
CCDSi | CCDS4730.1 [P18615-1] |
PIRi | S36789 |
RefSeqi | NP_002895.3, NM_002904.5 [P18615-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33231 , M32274, M32275, M32276, M33230 Genomic DNA Translation: AAA36308.1 L03411 mRNA Translation: AAC37523.1 AF019413 Genomic DNA Translation: AAB67979.1 AK300717 mRNA Translation: BAG62393.1 AK302652 mRNA Translation: BAG63891.1 AL049547 Genomic DNA Translation: CAB89308.1 AL662849 Genomic DNA No translation available. AL844853 Genomic DNA No translation available. BX005143 Genomic DNA No translation available. CR759782 Genomic DNA No translation available. CR388219 Genomic DNA No translation available. AL645922 Genomic DNA No translation available. CR753845 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03553.1 BC025235 mRNA Translation: AAH25235.1 BC050617 mRNA Translation: AAH50617.1 X16105 mRNA Translation: CAA34231.1 |
CCDSi | CCDS4730.1 [P18615-1] |
PIRi | S36789 |
RefSeqi | NP_002895.3, NM_002904.5 [P18615-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X5P | NMR | - | A | 254-337 | [»] | |
2BZ2 | NMR | - | A | 244-343 | [»] | |
2JX2 | NMR | - | A | 244-343 | [»] | |
5OOB | X-ray | 2.79 | E/K/Z | 360-380 | [»] | |
6GML | electron microscopy | 3.20 | X | 1-380 | [»] | |
BMRBi | P18615 | |||||
SMRi | P18615 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113662, 45 interactors |
CORUMi | P18615 |
DIPi | DIP-32669N |
IntActi | P18615, 30 interactors |
STRINGi | 9606.ENSP00000364578 |
PTM databases
iPTMneti | P18615 |
MetOSitei | P18615 |
PhosphoSitePlusi | P18615 |
Genetic variation databases
BioMutai | NELFE |
DMDMi | 1350554 |
Proteomic databases
CPTACi | CPTAC-999 |
EPDi | P18615 |
jPOSTi | P18615 |
MassIVEi | P18615 |
MaxQBi | P18615 |
PaxDbi | P18615 |
PeptideAtlasi | P18615 |
PRIDEi | P18615 |
ProteomicsDBi | 5199 53602 [P18615-1] 5554 |
Protocols and materials databases
Antibodypediai | 1924, 146 antibodies |
DNASUi | 7936 |
Genome annotation databases
Organism-specific databases
CTDi | 7936 |
DisGeNETi | 7936 |
GeneCardsi | NELFE |
HGNCi | HGNC:13974, NELFE |
HPAi | ENSG00000204356, Low tissue specificity |
MIMi | 154040, gene |
neXtProti | NX_P18615 |
OpenTargetsi | ENSG00000204356 |
PharmGKBi | PA134974984 |
VEuPathDBi | HostDB:ENSG00000204356.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQQ4, Eukaryota |
GeneTreei | ENSGT00630000089917 |
HOGENOMi | CLU_055643_0_0_1 |
InParanoidi | P18615 |
OMAi | RRQPIIE |
PhylomeDBi | P18615 |
TreeFami | TF324087 |
Enzyme and pathway databases
PathwayCommonsi | P18615 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-113418, Formation of the Early Elongation Complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167158, Formation of the HIV-1 Early Elongation Complex R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
BioGRID-ORCSi | 7936, 363 hits in 882 CRISPR screens |
EvolutionaryTracei | P18615 |
GeneWikii | RDBP |
GenomeRNAii | 7936 |
Pharosi | P18615, Tbio |
PROi | PR:P18615 |
RNActi | P18615, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204356, Expressed in testis and 237 other tissues |
ExpressionAtlasi | P18615, baseline and differential |
Genevisiblei | P18615, HS |
Family and domain databases
CDDi | cd12305, RRM_NELFE, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
IDEALi | IID00030 |
InterProi | View protein in InterPro IPR033102, NELFE IPR034637, NELFE_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR17250, PTHR17250, 2 hits |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NELFE_HUMAN | |
Accessioni | P18615Primary (citable) accession number: P18615 Secondary accession number(s): A2BE08 Q9NPK2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 225 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families