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UniProtKB - P18615 (NELFE_HUMAN)

Protein

Negative elongation factor E

Gene

NELFE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.Curated5 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor E
Short name:
NELF-E
Alternative name(s):
RNA-binding protein RD
Gene namesi
Name:NELFE
Synonyms:RD, RDBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204356.11
HGNCiHGNC:13974 NELFE
MIMi154040 gene
neXtProtiNX_P18615

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi295 – 299RNCAF → EQMAT: Abolishes interaction with RNA but not the interaction with other proteins of the NELF complex. 1 Publication5

Organism-specific databases

DisGeNETi7936
OpenTargetsiENSG00000204356
PharmGKBiPA134974984

Polymorphism and mutation databases

BioMutaiNELFE
DMDMi1350554

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818021 – 380Negative elongation factor EAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineCombined sources1
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei113PhosphoserineCombined sources1
Modified residuei115PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei251PhosphoserineCombined sources1
Modified residuei272PhosphothreonineCombined sources1
Modified residuei274PhosphothreonineCombined sources1
Modified residuei281PhosphoserineCombined sources1
Modified residuei353PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP18615
MaxQBiP18615
PaxDbiP18615
PeptideAtlasiP18615
PRIDEiP18615
ProteomicsDBi53602

PTM databases

iPTMnetiP18615
PhosphoSitePlusiP18615

Expressioni

Tissue specificityi

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000204356 Expressed in 224 organ(s), highest expression level in testis
CleanExiHS_RDBP
ExpressionAtlasiP18615 baseline and differential
GenevisibleiP18615 HS

Organism-specific databases

HPAiHPA007187
HPA007594
HPA046502

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi113662, 37 interactors
CORUMiP18615
DIPiDIP-32669N
IntActiP18615, 20 interactors
STRINGi9606.ENSP00000364578

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP18615
SMRiP18615
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18615

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati184 – 18512
Repeati186 – 18722
Repeati188 – 18932
Repeati190 – 19142
Repeati192 – 1935; approximate2
Repeati194 – 19562
Repeati196 – 19772
Repeati198 – 19982
Repeati200 – 20192
Repeati202 – 203102
Repeati204 – 205112
Repeati206 – 207122
Repeati208 – 209132
Repeati210 – 211142
Repeati212 – 213152
Repeati214 – 215162
Repeati216 – 217172
Repeati218 – 219182
Repeati220 – 221192
Repeati222 – 223202
Repeati224 – 225212
Repeati226 – 227222
Repeati228 – 229232
Repeati230 – 231242
Repeati232 – 233252
Repeati234 – 235262
Repeati236 – 237272
Repeati238 – 239282
Repeati240 – 241292
Repeati242 – 243302
Domaini262 – 332RRMPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 24330 X 2 AA approximate tandem repeats of R-[DSNE]Add BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili7 – 36Sequence analysisAdd BLAST30

Domaini

The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.1 Publication

Sequence similaritiesi

Belongs to the RRM NELF-E family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK4K Eukaryota
ENOG41101UX LUCA
GeneTreeiENSGT00630000089917
HOGENOMiHOG000232089
HOVERGENiHBG052598
InParanoidiP18615
KOiK15182
OMAiRRQPIIE
OrthoDBiEOG091G0I1Z
PhylomeDBiP18615
TreeFamiTF324087

Family and domain databases

CDDicd12305 RRM_NELFE, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR033102 NELFE
IPR034637 NELFE_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR17250 PTHR17250, 2 hits
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P18615-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL 
        60         70         80         90        100
SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE 
       110        120        130        140        150
KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG 
       160        170        180        190        200
EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR 
       210        220        230        240        250
DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD 
       260        270        280        290        300
SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV 
       310        320        330        340        350
TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV 
       360        370        380
QNSPKGCHRD KRTQIVYSDD VYKENLVDGF
Length:380
Mass (Da):43,240
Last modified:February 1, 1996 - v3
Checksum:i95EB422B5E07410B
GO
Isoform 2 (identifier: P18615-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPKGATM

Note: No experimental confirmation available.
Show »
Length:387
Mass (Da):43,925
Checksum:iCF6DCF317D74A6ED
GO
Isoform 3 (identifier: P18615-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-164: Missing.

Note: No experimental confirmation available.
Show »
Length:350
Mass (Da):40,216
Checksum:iD3066C12B041493F
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MSN9A0A0A0MSN9_HUMAN
Negative elongation factor E
NELFE
333Annotation score:
A2ABK4A2ABK4_HUMAN
Negative elongation factor E
NELFE
159Annotation score:
E9PD43E9PD43_HUMAN
Negative elongation factor E
NELFE
260Annotation score:
A0A0A0MT02A0A0A0MT02_HUMAN
Negative elongation factor E
NELFE
293Annotation score:
A0A0G2JI50A0A0G2JI50_HUMAN
Negative elongation factor E
NELFE
240Annotation score:
E7EWR7E7EWR7_HUMAN
Negative elongation factor E
NELFE
57Annotation score:
E7ENC7E7ENC7_HUMAN
Negative elongation factor E
NELFE
36Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119D → DD in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti174S → M in CAA34231 (PubMed:2119325).Curated1
Sequence conflicti265Y → YD in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti312V → A in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti327V → D in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti347S → A in AAA36308 (PubMed:2612324).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561511M → MVPKGATM in isoform 2. 1 Publication1
Alternative sequenceiVSP_056152135 – 164Missing in isoform 3. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33231
, M32274, M32275, M32276, M33230 Genomic DNA Translation: AAA36308.1
L03411 mRNA Translation: AAC37523.1
AF019413 Genomic DNA Translation: AAB67979.1
AK300717 mRNA Translation: BAG62393.1
AK302652 mRNA Translation: BAG63891.1
AL049547 Genomic DNA Translation: CAB89308.1
AL662849 Genomic DNA No translation available.
AL844853 Genomic DNA No translation available.
BX005143 Genomic DNA No translation available.
CR759782 Genomic DNA No translation available.
CR388219 Genomic DNA No translation available.
AL645922 Genomic DNA No translation available.
CR753845 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03553.1
BC025235 mRNA Translation: AAH25235.1
BC050617 mRNA Translation: AAH50617.1
X16105 mRNA Translation: CAA34231.1
CCDSiCCDS4730.1 [P18615-1]
PIRiS36789
RefSeqiNP_002895.3, NM_002904.5 [P18615-1]
UniGeneiHs.423935

Genome annotation databases

EnsembliENST00000375425; ENSP00000364574; ENSG00000204356 [P18615-3]
ENST00000375429; ENSP00000364578; ENSG00000204356 [P18615-1]
ENST00000383174; ENSP00000372660; ENSG00000206268 [P18615-1]
ENST00000383343; ENSP00000372834; ENSG00000206357 [P18615-1]
ENST00000429857; ENSP00000403623; ENSG00000231044 [P18615-1]
ENST00000444811; ENSP00000388400; ENSG00000204356 [P18615-4]
ENST00000448628; ENSP00000394879; ENSG00000229363 [P18615-1]
ENST00000457397; ENSP00000393005; ENSG00000233801 [P18615-1]
GeneIDi7936
KEGGihsa:7936
UCSCiuc003nyk.4 human [P18615-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33231
, M32274, M32275, M32276, M33230 Genomic DNA Translation: AAA36308.1
L03411 mRNA Translation: AAC37523.1
AF019413 Genomic DNA Translation: AAB67979.1
AK300717 mRNA Translation: BAG62393.1
AK302652 mRNA Translation: BAG63891.1
AL049547 Genomic DNA Translation: CAB89308.1
AL662849 Genomic DNA No translation available.
AL844853 Genomic DNA No translation available.
BX005143 Genomic DNA No translation available.
CR759782 Genomic DNA No translation available.
CR388219 Genomic DNA No translation available.
AL645922 Genomic DNA No translation available.
CR753845 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03553.1
BC025235 mRNA Translation: AAH25235.1
BC050617 mRNA Translation: AAH50617.1
X16105 mRNA Translation: CAA34231.1
CCDSiCCDS4730.1 [P18615-1]
PIRiS36789
RefSeqiNP_002895.3, NM_002904.5 [P18615-1]
UniGeneiHs.423935

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X5PNMR-A254-337[»]
2BZ2NMR-A244-343[»]
2JX2NMR-A244-343[»]
5OOBX-ray2.79E/K/Z360-380[»]
6GMLelectron microscopy3.20X1-380[»]
ProteinModelPortaliP18615
SMRiP18615
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113662, 37 interactors
CORUMiP18615
DIPiDIP-32669N
IntActiP18615, 20 interactors
STRINGi9606.ENSP00000364578

PTM databases

iPTMnetiP18615
PhosphoSitePlusiP18615

Polymorphism and mutation databases

BioMutaiNELFE
DMDMi1350554

Proteomic databases

EPDiP18615
MaxQBiP18615
PaxDbiP18615
PeptideAtlasiP18615
PRIDEiP18615
ProteomicsDBi53602

Protocols and materials databases

DNASUi7936
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375425; ENSP00000364574; ENSG00000204356 [P18615-3]
ENST00000375429; ENSP00000364578; ENSG00000204356 [P18615-1]
ENST00000383174; ENSP00000372660; ENSG00000206268 [P18615-1]
ENST00000383343; ENSP00000372834; ENSG00000206357 [P18615-1]
ENST00000429857; ENSP00000403623; ENSG00000231044 [P18615-1]
ENST00000444811; ENSP00000388400; ENSG00000204356 [P18615-4]
ENST00000448628; ENSP00000394879; ENSG00000229363 [P18615-1]
ENST00000457397; ENSP00000393005; ENSG00000233801 [P18615-1]
GeneIDi7936
KEGGihsa:7936
UCSCiuc003nyk.4 human [P18615-1]

Organism-specific databases

CTDi7936
DisGeNETi7936
EuPathDBiHostDB:ENSG00000204356.11
GeneCardsiNELFE
HGNCiHGNC:13974 NELFE
HPAiHPA007187
HPA007594
HPA046502
MIMi154040 gene
neXtProtiNX_P18615
OpenTargetsiENSG00000204356
PharmGKBiPA134974984
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK4K Eukaryota
ENOG41101UX LUCA
GeneTreeiENSGT00630000089917
HOGENOMiHOG000232089
HOVERGENiHBG052598
InParanoidiP18615
KOiK15182
OMAiRRQPIIE
OrthoDBiEOG091G0I1Z
PhylomeDBiP18615
TreeFamiTF324087

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

EvolutionaryTraceiP18615
GeneWikiiRDBP
GenomeRNAii7936
PROiPR:P18615
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204356 Expressed in 224 organ(s), highest expression level in testis
CleanExiHS_RDBP
ExpressionAtlasiP18615 baseline and differential
GenevisibleiP18615 HS

Family and domain databases

CDDicd12305 RRM_NELFE, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR033102 NELFE
IPR034637 NELFE_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR17250 PTHR17250, 2 hits
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNELFE_HUMAN
AccessioniPrimary (citable) accession number: P18615
Secondary accession number(s): A2BE08
, B4DUN1, B4DYX9, Q5JP74, Q5JP75, Q96F56, Q9NPK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 210 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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