Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
We will be switching to the new UniProt website soon. Please explore and share your feedback. Take me to the new website.

UniProtKB - P18615 (NELFE_HUMAN)

Entry version 229 (23 Feb 2022)
Sequence version 3 (01 Feb 1996)
Previous versions | rss
Add a publicationFeedback
Protein

Negative elongation factor E

Gene

NELFE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401).

The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062).

Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27282391).

5 Publications

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P18615

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-112382, Formation of RNA Pol II elongation complex
R-HSA-113418, Formation of the Early Elongation Complex
R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158, Formation of the HIV-1 Early Elongation Complex
R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243, Tat-mediated HIV elongation arrest and recovery
R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287, HIV elongation arrest and recovery
R-HSA-167290, Pausing and recovery of HIV elongation
R-HSA-674695, RNA Polymerase II Pre-transcription Events
R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955, RNA Polymerase II Transcription Elongation

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		P18615

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Negative elongation factor E
Short name:
NELF-E
Alternative name(s):
RNA-binding protein RD
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NELFE
Synonyms:RD, RDBP
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 6

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:13974, NELFE

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		154040, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P18615

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000204356

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi295 – 299RNCAF → EQMAT: Abolishes interaction with RNA but not the interaction with other proteins of the NELF complex. 1 Publication5

Organism-specific databases

DisGeNET

More...DisGeNETi		7936

Open Targets

More...OpenTargetsi		ENSG00000204356

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA134974984

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P18615, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		NELFE

Domain mapping of disease mutations (DMDM)

More...DMDMi		1350554

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000818021 – 380Negative elongation factor EAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei51PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei113PhosphoserineCombined sources1
Modified residuei115PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei251PhosphoserineCombined sources1
Modified residuei272PhosphothreonineCombined sources1
Modified residuei274PhosphothreonineCombined sources1
Modified residuei281PhosphoserineCombined sources1
Modified residuei353PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

The CPTAC Assay portal

More...CPTACi		CPTAC-999

Encyclopedia of Proteome Dynamics

More...EPDi		P18615

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P18615

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P18615

MaxQB - The MaxQuant DataBase

More...MaxQBi		P18615

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P18615

PeptideAtlas

More...PeptideAtlasi		P18615

PRoteomics IDEntifications database

More...PRIDEi		P18615

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		5199
53602 [P18615-1]
5554

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		P18615, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P18615

MetOSite database of methionine sulfoxide sites

More...MetOSitei		P18615

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P18615

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000204356, Expressed in testis and 238 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P18615, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P18615, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000204356, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE (PubMed:10199401, PubMed:27282391).

Interacts with NELFB (By similarity).

By similarity2 Publications

(Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		113662, 61 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		P18615

Database of interacting proteins

More...DIPi		DIP-32669N

Protein interaction database and analysis system

More...IntActi		P18615, 31 interactors

Molecular INTeraction database

More...MINTi		P18615

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000364578

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P18615, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P18615

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P18615

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P18615

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati184 – 18512
Repeati186 – 18722
Repeati188 – 18932
Repeati190 – 19142
Repeati192 – 1935; approximate2
Repeati194 – 19562
Repeati196 – 19772
Repeati198 – 19982
Repeati200 – 20192
Repeati202 – 203102
Repeati204 – 205112
Repeati206 – 207122
Repeati208 – 209132
Repeati210 – 211142
Repeati212 – 213152
Repeati214 – 215162
Repeati216 – 217172
Repeati218 – 219182
Repeati220 – 221192
Repeati222 – 223202
Repeati224 – 225212
Repeati226 – 227222
Repeati228 – 229232
Repeati230 – 231242
Repeati232 – 233252
Repeati234 – 235262
Repeati236 – 237272
Repeati238 – 239282
Repeati240 – 241292
Repeati242 – 243302
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini262 – 332RRMPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni30 – 67DisorderedSequence analysisAdd BLAST38
Regioni79 – 258DisorderedSequence analysisAdd BLAST180
Regioni184 – 24330 X 2 AA approximate tandem repeats of R-[DSNE]Add BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili7 – 36Sequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi32 – 67Polar residuesSequence analysisAdd BLAST36
Compositional biasi83 – 100Basic and acidic residuesSequence analysisAdd BLAST18
Compositional biasi183 – 257Basic and acidic residuesSequence analysisAdd BLAST75

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the RRM NELF-E family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502QQQ4, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00630000089917

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_055643_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P18615

Database for complete collections of gene phylogenies

More...PhylomeDBi		P18615

TreeFam database of animal gene trees

More...TreeFami		TF324087

Family and domain databases

Conserved Domains Database

More...CDDi		cd12305, RRM_NELFE, 1 hit

Database of protein disorder

More...DisProti		DP02713

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.70.330, 1 hit

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...IDEALi		IID00030

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR033102, NELFE
IPR034637, NELFE_RRM
IPR012677, Nucleotide-bd_a/b_plait_sf
IPR035979, RBD_domain_sf
IPR000504, RRM_dom

The PANTHER Classification System

More...PANTHERi		PTHR17250, PTHR17250, 2 hits

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00076, RRM_1, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00360, RRM, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF54928, SSF54928, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50102, RRM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P18615-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL 
        60         70         80         90        100
SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE 
       110        120        130        140        150
KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG 
       160        170        180        190        200
EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR 
       210        220        230        240        250
DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD 
       260        270        280        290        300
SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV 
       310        320        330        340        350
TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV 
       360        370        380
QNSPKGCHRD KRTQIVYSDD VYKENLVDGF
Length:380
Mass (Da):43,240
Last modified:February 1, 1996 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i95EB422B5E07410B
GO
Isoform 2 (identifier: P18615-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPKGATM

Show »
Length:387
Mass (Da):43,925
Checksum:iCF6DCF317D74A6ED
GO
Isoform 3 (identifier: P18615-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-164: Missing.

Show »
Length:350
Mass (Da):40,216
Checksum:iD3066C12B041493F
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MSN9A0A0A0MSN9_HUMAN
Negative elongation factor E
NELFE
333Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PD43E9PD43_HUMAN
Negative elongation factor E
NELFE
260Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2ABK4A2ABK4_HUMAN
Negative elongation factor E
NELFE
159Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0A0MT02A0A0A0MT02_HUMAN
Negative elongation factor E
NELFE
293Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0G2JI50A0A0G2JI50_HUMAN
Negative elongation factor E
NELFE
240Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E7EWR7E7EWR7_HUMAN
Negative elongation factor E
NELFE
57Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E7ENC7E7ENC7_HUMAN
Negative elongation factor E
NELFE
36Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti119D → DD in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti174S → M in CAA34231 (PubMed:2119325).Curated1
Sequence conflicti265Y → YD in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti312V → A in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti327V → D in AAA36308 (PubMed:2612324).Curated1
Sequence conflicti347S → A in AAA36308 (PubMed:2612324).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0561511M → MVPKGATM in isoform 2. 1 Publication1
Alternative sequenceiVSP_056152135 – 164Missing in isoform 3. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M33231 M33230 Genomic DNA Translation: AAA36308.1
L03411 mRNA Translation: AAC37523.1
AF019413 Genomic DNA Translation: AAB67979.1
AK300717 mRNA Translation: BAG62393.1
AK302652 mRNA Translation: BAG63891.1
AL049547 Genomic DNA Translation: CAB89308.1
AL662849 Genomic DNA No translation available.
AL844853 Genomic DNA No translation available.
BX005143 Genomic DNA No translation available.
CR759782 Genomic DNA No translation available.
CR388219 Genomic DNA No translation available.
AL645922 Genomic DNA No translation available.
CR753845 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03553.1
BC025235 mRNA Translation: AAH25235.1
BC050617 mRNA Translation: AAH50617.1
X16105 mRNA Translation: CAA34231.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS4730.1 [P18615-1]

Protein sequence database of the Protein Information Resource

More...PIRi		S36789

NCBI Reference Sequences

More...RefSeqi		NP_002895.3, NM_002904.5 [P18615-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000375425; ENSP00000364574; ENSG00000204356 [P18615-3]
ENST00000375429; ENSP00000364578; ENSG00000204356
ENST00000383174; ENSP00000372660; ENSG00000206268
ENST00000383343; ENSP00000372834; ENSG00000206357
ENST00000429857; ENSP00000403623; ENSG00000231044
ENST00000444811; ENSP00000388400; ENSG00000204356 [P18615-4]
ENST00000448628; ENSP00000394879; ENSG00000229363
ENST00000457397; ENSP00000393005; ENSG00000233801

Database of genes from NCBI RefSeq genomes

More...GeneIDi		7936

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:7936

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000375429.8; ENSP00000364578.3; NM_002904.6; NP_002895.3

UCSC genome browser

More...UCSCi		uc003nyk.4, human [P18615-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33231 M33230 Genomic DNA Translation: AAA36308.1
L03411 mRNA Translation: AAC37523.1
AF019413 Genomic DNA Translation: AAB67979.1
AK300717 mRNA Translation: BAG62393.1
AK302652 mRNA Translation: BAG63891.1
AL049547 Genomic DNA Translation: CAB89308.1
AL662849 Genomic DNA No translation available.
AL844853 Genomic DNA No translation available.
BX005143 Genomic DNA No translation available.
CR759782 Genomic DNA No translation available.
CR388219 Genomic DNA No translation available.
AL645922 Genomic DNA No translation available.
CR753845 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03553.1
BC025235 mRNA Translation: AAH25235.1
BC050617 mRNA Translation: AAH50617.1
X16105 mRNA Translation: CAA34231.1
CCDSiCCDS4730.1 [P18615-1]
PIRiS36789
RefSeqiNP_002895.3, NM_002904.5 [P18615-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X5PNMR-A254-337[»]
2BZ2NMR-A244-343[»]
2JX2NMR-A244-343[»]
5OOBX-ray2.79E/K/Z360-380[»]
6GMLelectron microscopy3.20X1-380[»]
BMRBiP18615
SMRiP18615
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi113662, 61 interactors
CORUMiP18615
DIPiDIP-32669N
IntActiP18615, 31 interactors
MINTiP18615
STRINGi9606.ENSP00000364578

PTM databases

GlyGeniP18615, 1 site, 1 O-linked glycan (1 site)
iPTMnetiP18615
MetOSiteiP18615
PhosphoSitePlusiP18615

Genetic variation databases

BioMutaiNELFE
DMDMi1350554

Proteomic databases

CPTACiCPTAC-999
EPDiP18615
jPOSTiP18615
MassIVEiP18615
MaxQBiP18615
PaxDbiP18615
PeptideAtlasiP18615
PRIDEiP18615
ProteomicsDBi5199
53602 [P18615-1]
5554

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		1924, 153 antibodies from 27 providers

The DNASU plasmid repository

More...DNASUi		7936

Genome annotation databases

EnsembliENST00000375425; ENSP00000364574; ENSG00000204356 [P18615-3]
ENST00000375429; ENSP00000364578; ENSG00000204356
ENST00000383174; ENSP00000372660; ENSG00000206268
ENST00000383343; ENSP00000372834; ENSG00000206357
ENST00000429857; ENSP00000403623; ENSG00000231044
ENST00000444811; ENSP00000388400; ENSG00000204356 [P18615-4]
ENST00000448628; ENSP00000394879; ENSG00000229363
ENST00000457397; ENSP00000393005; ENSG00000233801
GeneIDi7936
KEGGihsa:7936
MANE-SelectiENST00000375429.8; ENSP00000364578.3; NM_002904.6; NP_002895.3
UCSCiuc003nyk.4, human [P18615-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		7936
DisGeNETi7936

GeneCards: human genes, protein and diseases

More...GeneCardsi		NELFE
HGNCiHGNC:13974, NELFE
HPAiENSG00000204356, Low tissue specificity
MIMi154040, gene
neXtProtiNX_P18615
OpenTargetsiENSG00000204356
PharmGKBiPA134974984
VEuPathDBiHostDB:ENSG00000204356

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiENOG502QQQ4, Eukaryota
GeneTreeiENSGT00630000089917
HOGENOMiCLU_055643_0_0_1
InParanoidiP18615
PhylomeDBiP18615
TreeFamiTF324087

Enzyme and pathway databases

PathwayCommonsiP18615
ReactomeiR-HSA-112382, Formation of RNA Pol II elongation complex
R-HSA-113418, Formation of the Early Elongation Complex
R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158, Formation of the HIV-1 Early Elongation Complex
R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243, Tat-mediated HIV elongation arrest and recovery
R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287, HIV elongation arrest and recovery
R-HSA-167290, Pausing and recovery of HIV elongation
R-HSA-674695, RNA Polymerase II Pre-transcription Events
R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955, RNA Polymerase II Transcription Elongation
SignaLinkiP18615

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		7936, 372 hits in 1050 CRISPR screens
EvolutionaryTraceiP18615

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		RDBP

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		7936
PharosiP18615, Tbio

Protein Ontology

More...PROi		PR:P18615
RNActiP18615, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000204356, Expressed in testis and 238 other tissues
ExpressionAtlasiP18615, baseline and differential
GenevisibleiP18615, HS

Family and domain databases

CDDicd12305, RRM_NELFE, 1 hit
DisProtiDP02713
Gene3Di3.30.70.330, 1 hit
IDEALiIID00030
InterProiView protein in InterPro
IPR033102, NELFE
IPR034637, NELFE_RRM
IPR012677, Nucleotide-bd_a/b_plait_sf
IPR035979, RBD_domain_sf
IPR000504, RRM_dom
PANTHERiPTHR17250, PTHR17250, 2 hits
PfamiView protein in Pfam
PF00076, RRM_1, 1 hit
SMARTiView protein in SMART
SM00360, RRM, 1 hit
SUPFAMiSSF54928, SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102, RRM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNELFE_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P18615
Secondary accession number(s): A2BE08
, B4DUN1, B4DYX9, Q5JP74, Q5JP75, Q96F56, Q9NPK2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: February 23, 2022
This is version 229 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again