ARG8

Functionⁱ

Miscellaneous

The reaction catalyzed by ACOAT is highly reversible. Moreover this enzyme may transaminate ornithine.

Present with 2300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

N₂-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.

Cofactorⁱ

pyridoxal 5'-phosphate

Pathwayⁱ: L-arginine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

Amino-acid acetyltransferase, mitochondrial (ARG2), Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (ARG7)
Protein ARG5,6, mitochondrial (ARG5,6)
Protein ARG5,6, mitochondrial (ARG5,6)
Acetylornithine aminotransferase, mitochondrial (ARG8)

This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functionⁱ

identical protein binding Source: GO_Central
N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity
Source: SGD
Inferred from direct assayⁱ
- 2199330
pyridoxal phosphate binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminotransferase, Transferase
Biological process	Amino-acid biosynthesis, Arginine biosynthesis
Ligand	Pyridoxal phosphate

Enzyme and pathway databases

BioCycⁱ	YEAST:YOL140W-MONOMER
Reactomeⁱ	R-SCE-1442490 Collagen degradation R-SCE-1483213 Synthesis of PE R-SCE-389661 Glyoxylate metabolism and glycine degradation R-SCE-71064 Lysine catabolism
UniPathwayⁱ	UPA00068; UER00109

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Acetylornithine aminotransferase, mitochondrial (EC:2.6.1.11) Short name: ACOAT
Gene namesⁱ	Name:ARG8 Ordered Locus Names:YOL140W
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XV

Organism-specific databases

EuPathDBⁱ	FungiDB:YOL140W
Saccharomyces Genome Database More...SGDⁱ	S000005500 ARG8

Keywords - Cellular componentⁱ

Mitochondrion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000002083	? – 423	Acetylornithine aminotransferase, mitochondrial
Transit peptideⁱ	1 – ?	MitochondrionSequence analysis

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	276	N6-(pyridoxal phosphate)lysineBy similarity		1

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P18544
PaxDbⁱ	P18544
PRIDEⁱ	P18544

Interactionⁱ

GO - Molecular functionⁱ

identical protein binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	34277, 11 interactors
Database of interacting proteins More...DIPⁱ	DIP-2623N
IntActⁱ	P18544, 1 interactor
Molecular INTeraction database More...MINTⁱ	P18544
STRINGⁱ	4932.YOL140W

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P18544
SMRⁱ	P18544
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.Curated

Keywords - Domainⁱ

Transit peptide

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062907
HOGENOMⁱ	HOG000020206
InParanoidⁱ	P18544
KEGG Orthology (KO) More...KOⁱ	K00818
OMAⁱ	GIATCTL
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2QO7

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00610 OAT_like, 1 hit
Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 2 hits
HAMAPⁱ	MF_01107 ArgD_aminotrans_3, 1 hit
InterProⁱ	View protein in InterPro IPR004636 AcOrn/SuccOrn_fam IPR005814 Aminotrans_3 IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00202 Aminotran_3, 1 hit
PIRSFⁱ	PIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
TIGRFAMsⁱ	TIGR00707 argD, 1 hit
PROSITEⁱ	View protein in PROSITE PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P18544-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFKRYLSSTS SRRFTSILEE KAFQVTTYSR PEDLCITRGK NAKLYDDVNG 
        60         70         80         90        100
KEYIDFTAGI AVTALGHANP KVAEILHHQA NKLVHSSNLY FTKECLDLSE 
       110        120        130        140        150
KIVEKTKQFG GQHDASRVFL CNSGTEANEA ALKFAKKHGI MKNPSKQGIV 
       160        170        180        190        200
AFENSFHGRT MGALSVTWNS KYRTPFGDLV PHVSFLNLND EMTKLQSYIE 
       210        220        230        240        250
TKKDEIAGLI VEPIQGEGGV FPVEVEKLTG LKKICQDNDV IVIHDEIQCG 
       260        270        280        290        300
LGRSGKLWAH AYLPSEAHPD IFTSAKALGN GFPIAATIVN EKVNNALRVG 
       310        320        330        340        350
DHGTTYGGNP LACSVSNYVL DTIADEAFLK QVSKKSDILQ KRLREIQAKY 
       360        370        380        390        400
PNQIKTIRGK GLMLGAEFVE PPTEVIKKAR ELGLLIITAG KSTVRFVPAL 
       410        420 
TIEDELIEEG MDAFEKAIEA VYA

Length:423

Mass (Da):46,681

Last modified:November 1, 1990 - v1

Checksum:ⁱ0F468560865629DC

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M32795 Genomic DNA Translation: AAA34436.1 X84036 Genomic DNA Translation: CAA58853.1 Z74882 Genomic DNA Translation: CAA99161.1 BK006948 Genomic DNA Translation: DAA10645.1
PIRⁱ	S61868
NCBI Reference Sequences More...RefSeqⁱ	NP_014501.1, NM_001183394.1

Genome annotation databases

EnsemblFungiⁱ	YOL140W; YOL140W; YOL140W
GeneIDⁱ	854025
KEGGⁱ	sce:YOL140W

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P18544	K7_Arg8p		YEASK		423
Arg8p		YEASC		423
Arg8p		YEASA		451
Acetylornithine aminotransferase		YEAS1		423
Arg8p		YEAS8		423
+10

Protein	Similar proteins		Species	Score	Length
P18544	K7_Arg8p		YEASK		423
Arg8p		YEASC		423
Arg8p		YEASL		451
Arg8p		YEASA		451
Acetylornithine aminotransferase		YEAS1		423
+21

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M32795 Genomic DNA Translation: AAA34436.1 X84036 Genomic DNA Translation: CAA58853.1 Z74882 Genomic DNA Translation: CAA99161.1 BK006948 Genomic DNA Translation: DAA10645.1
PIRⁱ	S61868
NCBI Reference Sequences More...RefSeqⁱ	NP_014501.1, NM_001183394.1

3D structure databases

ProteinModelPortalⁱ	P18544
SMRⁱ	P18544
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	34277, 11 interactors
Database of interacting proteins More...DIPⁱ	DIP-2623N
IntActⁱ	P18544, 1 interactor
Molecular INTeraction database More...MINTⁱ	P18544
STRINGⁱ	4932.YOL140W

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P18544
PaxDbⁱ	P18544
PRIDEⁱ	P18544

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YOL140W; YOL140W; YOL140W
GeneIDⁱ	854025
KEGGⁱ	sce:YOL140W

Organism-specific databases

EuPathDBⁱ	FungiDB:YOL140W
Saccharomyces Genome Database More...SGDⁱ	S000005500 ARG8

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062907
HOGENOMⁱ	HOG000020206
InParanoidⁱ	P18544
KEGG Orthology (KO) More...KOⁱ	K00818
OMAⁱ	GIATCTL
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2QO7

Enzyme and pathway databases

UniPathwayⁱ	UPA00068; UER00109
BioCycⁱ	YEAST:YOL140W-MONOMER
Reactomeⁱ	R-SCE-1442490 Collagen degradation R-SCE-1483213 Synthesis of PE R-SCE-389661 Glyoxylate metabolism and glycine degradation R-SCE-71064 Lysine catabolism

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P18544

PROⁱ

PR:P18544

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00610 OAT_like, 1 hit
Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 2 hits
HAMAPⁱ	MF_01107 ArgD_aminotrans_3, 1 hit
InterProⁱ	View protein in InterPro IPR004636 AcOrn/SuccOrn_fam IPR005814 Aminotrans_3 IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00202 Aminotran_3, 1 hit
PIRSFⁱ	PIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
TIGRFAMsⁱ	TIGR00707 argD, 1 hit
PROSITEⁱ	View protein in PROSITE PS00600 AA_TRANSFER_CLASS_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ARGD_YEAST
Accessionⁱ	P18544Primary (citable) accession number: P18544 Secondary accession number(s): D6W1S9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
	Last sequence update:	November 1, 1990
	Last modified:	May 23, 2018
	This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P18544 (ARGD_YEAST)

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Acetylornithine aminotransferase, mitochondrial

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-arginine biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: L-arginine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ