UniProtKB - P18544 (ARGD_YEAST)
Protein
Acetylornithine aminotransferase, mitochondrial
Gene
ARG8
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
The reaction catalyzed by ACOAT is highly reversible. Moreover this enzyme may transaminate ornithine.
Present with 2300 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.6.1.11
Cofactori
: L-arginine biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (ARG7), Amino-acid acetyltransferase, mitochondrial (ARG2)
- Protein ARG5,6, mitochondrial (ARG5,6)
- Protein ARG5,6, mitochondrial (ARG5,6)
- Acetylornithine aminotransferase, mitochondrial (ARG8)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- identical protein binding Source: GO_Central
- N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Source: SGD
- pyridoxal phosphate binding Source: GO_Central
GO - Biological processi
- arginine biosynthetic process Source: SGD
- ornithine biosynthetic process Source: SGD
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00109 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ARG8 Ordered Locus Names:YOL140W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOL140W |
SGDi | S000005500, ARG8 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000002083 | ? – 423 | Acetylornithine aminotransferase, mitochondrial |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 276 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
MaxQBi | P18544 |
PaxDbi | P18544 |
PRIDEi | P18544 |
Interactioni
GO - Molecular functioni
- identical protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 34277, 12 interactors |
DIPi | DIP-2623N |
IntActi | P18544, 1 interactor |
MINTi | P18544 |
STRINGi | 4932.YOL140W |
Miscellaneous databases
RNActi | P18544, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1401, Eukaryota |
GeneTreei | ENSGT00970000196319 |
HOGENOMi | CLU_016922_10_1_1 |
InParanoidi | P18544 |
OMAi | GWGLING |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01107, ArgD_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR004636, AcOrn/SuccOrn_fam IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00707, argD, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P18544-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKRYLSSTS SRRFTSILEE KAFQVTTYSR PEDLCITRGK NAKLYDDVNG
60 70 80 90 100
KEYIDFTAGI AVTALGHANP KVAEILHHQA NKLVHSSNLY FTKECLDLSE
110 120 130 140 150
KIVEKTKQFG GQHDASRVFL CNSGTEANEA ALKFAKKHGI MKNPSKQGIV
160 170 180 190 200
AFENSFHGRT MGALSVTWNS KYRTPFGDLV PHVSFLNLND EMTKLQSYIE
210 220 230 240 250
TKKDEIAGLI VEPIQGEGGV FPVEVEKLTG LKKICQDNDV IVIHDEIQCG
260 270 280 290 300
LGRSGKLWAH AYLPSEAHPD IFTSAKALGN GFPIAATIVN EKVNNALRVG
310 320 330 340 350
DHGTTYGGNP LACSVSNYVL DTIADEAFLK QVSKKSDILQ KRLREIQAKY
360 370 380 390 400
PNQIKTIRGK GLMLGAEFVE PPTEVIKKAR ELGLLIITAG KSTVRFVPAL
410 420
TIEDELIEEG MDAFEKAIEA VYA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32795 Genomic DNA Translation: AAA34436.1 X84036 Genomic DNA Translation: CAA58853.1 Z74882 Genomic DNA Translation: CAA99161.1 BK006948 Genomic DNA Translation: DAA10645.1 |
PIRi | S61868 |
RefSeqi | NP_014501.1, NM_001183394.1 |
Genome annotation databases
EnsemblFungii | YOL140W_mRNA; YOL140W; YOL140W |
GeneIDi | 854025 |
KEGGi | sce:YOL140W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32795 Genomic DNA Translation: AAA34436.1 X84036 Genomic DNA Translation: CAA58853.1 Z74882 Genomic DNA Translation: CAA99161.1 BK006948 Genomic DNA Translation: DAA10645.1 |
PIRi | S61868 |
RefSeqi | NP_014501.1, NM_001183394.1 |
3D structure databases
SMRi | P18544 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34277, 12 interactors |
DIPi | DIP-2623N |
IntActi | P18544, 1 interactor |
MINTi | P18544 |
STRINGi | 4932.YOL140W |
Proteomic databases
MaxQBi | P18544 |
PaxDbi | P18544 |
PRIDEi | P18544 |
Genome annotation databases
EnsemblFungii | YOL140W_mRNA; YOL140W; YOL140W |
GeneIDi | 854025 |
KEGGi | sce:YOL140W |
Organism-specific databases
EuPathDBi | FungiDB:YOL140W |
SGDi | S000005500, ARG8 |
Phylogenomic databases
eggNOGi | KOG1401, Eukaryota |
GeneTreei | ENSGT00970000196319 |
HOGENOMi | CLU_016922_10_1_1 |
InParanoidi | P18544 |
OMAi | GWGLING |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00109 |
Miscellaneous databases
PROi | PR:P18544 |
RNActi | P18544, protein |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01107, ArgD_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR004636, AcOrn/SuccOrn_fam IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00707, argD, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARGD_YEAST | |
Accessioni | P18544Primary (citable) accession number: P18544 Secondary accession number(s): D6W1S9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD