We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P18494 (GLN3_YEAST)
Protein
Nitrogen regulatory protein GLN3
Gene
GLN3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.
Miscellaneous
Present with 589 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 306 – 330 | GATA-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: SGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: SGD
- sequence-specific DNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: SGD
- nitrate assimilation Source: UniProtKB-KW
- nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation Source: SGD
- regulation of protein localization by the Cvt pathway Source: SGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Nitrate assimilation, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrogen regulatory protein GLN3 |
Gene namesi | Name:GLN3 Ordered Locus Names:YER040W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000842, GLN3 |
VEuPathDBi | FungiDB:YER040W |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083477 | 1 – 730 | Nitrogen regulatory protein GLN3Add BLAST | 730 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 251 | PhosphoserineCombined sources | 1 | |
Modified residuei | 267 | PhosphoserineCombined sources | 1 | |
Modified residuei | 285 | PhosphoserineCombined sources | 1 | |
Modified residuei | 469 | PhosphoserineCombined sources | 1 | |
Modified residuei | 552 | PhosphoserineCombined sources | 1 | |
Modified residuei | 562 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P18494 |
PaxDbi | P18494 |
PRIDEi | P18494 |
PTM databases
iPTMneti | P18494 |
Interactioni
Binary interactionsi
P18494
With | #Exp. | IntAct |
---|---|---|
URE2 [P23202] | 2 | EBI-7657,EBI-20138 |
Protein-protein interaction databases
BioGRIDi | 36776, 425 interactors |
DIPi | DIP-2353N |
IntActi | P18494, 11 interactors |
MINTi | P18494 |
STRINGi | 4932.YER040W |
Miscellaneous databases
RNActi | P18494, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 45 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 187 – 208 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 224 – 262 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 355 – 398 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 449 – 516 | DisorderedSequence analysisAdd BLAST | 68 | |
Regioni | 593 – 673 | DisorderedSequence analysisAdd BLAST | 81 | |
Regioni | 696 – 717 | DisorderedSequence analysisAdd BLAST | 22 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 129 – 137 | 9aaTAD | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 31 – 45 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 361 – 398 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 702 – 716 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 306 – 330 | GATA-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1601, Eukaryota |
HOGENOMi | CLU_022036_0_0_1 |
InParanoidi | P18494 |
OMAi | IQCFNCK |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 1 hit |
Gene3Di | 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR039355, Transcription_factor_GATA IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR10071, PTHR10071, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 1 hit |
PRINTSi | PR00619, GATAZNFINGER |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 1 hit |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 1 hit PS50114, GATA_ZN_FINGER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P18494-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA
60 70 80 90 100
FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT
110 120 130 140 150
NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS
160 170 180 190 200
GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN
210 220 230 240 250
NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD
260 270 280 290 300
SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK
310 320 330 340 350
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI
360 370 380 390 400
KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS
410 420 430 440 450
LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN
460 470 480 490 500
FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV
510 520 530 540 550
VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS
560 570 580 590 600
NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD
610 620 630 640 650
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR
660 670 680 690 700
PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG
710 720 730
GKISEDNSTK GSSKESSAIA DELDWLKFGI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 474 | P → G in AAA34645 (PubMed:1682800).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35267 Genomic DNA Translation: AAA34645.1 U18796 Genomic DNA Translation: AAB64575.1 BK006939 Genomic DNA Translation: DAA07694.1 |
PIRi | S50543 |
RefSeqi | NP_010958.3, NM_001178931.3 |
Genome annotation databases
EnsemblFungii | YER040W_mRNA; YER040W; YER040W |
GeneIDi | 856763 |
KEGGi | sce:YER040W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35267 Genomic DNA Translation: AAA34645.1 U18796 Genomic DNA Translation: AAB64575.1 BK006939 Genomic DNA Translation: DAA07694.1 |
PIRi | S50543 |
RefSeqi | NP_010958.3, NM_001178931.3 |
3D structure databases
SMRi | P18494 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36776, 425 interactors |
DIPi | DIP-2353N |
IntActi | P18494, 11 interactors |
MINTi | P18494 |
STRINGi | 4932.YER040W |
PTM databases
iPTMneti | P18494 |
Proteomic databases
MaxQBi | P18494 |
PaxDbi | P18494 |
PRIDEi | P18494 |
Genome annotation databases
EnsemblFungii | YER040W_mRNA; YER040W; YER040W |
GeneIDi | 856763 |
KEGGi | sce:YER040W |
Organism-specific databases
SGDi | S000000842, GLN3 |
VEuPathDBi | FungiDB:YER040W |
Phylogenomic databases
eggNOGi | KOG1601, Eukaryota |
HOGENOMi | CLU_022036_0_0_1 |
InParanoidi | P18494 |
OMAi | IQCFNCK |
Enzyme and pathway databases
Reactomei | R-SCE-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
PROi | PR:P18494 |
RNActi | P18494, protein |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 1 hit |
Gene3Di | 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR039355, Transcription_factor_GATA IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR10071, PTHR10071, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 1 hit |
PRINTSi | PR00619, GATAZNFINGER |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 1 hit |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 1 hit PS50114, GATA_ZN_FINGER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLN3_YEAST | |
Accessioni | P18494Primary (citable) accession number: P18494 Secondary accession number(s): D3DLU0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names