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Protein

Nitrogen regulatory protein GLN3

Gene

GLN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri306 – 330GATA-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processNitrate assimilation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30221-MONOMER
ReactomeiR-SCE-5689880 Ub-specific processing proteases
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein GLN3
Gene namesi
Name:GLN3
Ordered Locus Names:YER040W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

SGDiS000000842 GLN3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834771 – 730Nitrogen regulatory protein GLN3Add BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei285PhosphoserineCombined sources1
Modified residuei469PhosphoserineCombined sources1
Modified residuei552PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18494
PaxDbiP18494
PRIDEiP18494

PTM databases

iPTMnetiP18494

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
URE2P232022EBI-7657,EBI-20138

Protein-protein interaction databases

BioGridi36776, 417 interactors
DIPiDIP-2353N
IntActiP18494, 11 interactors
MINTiP18494
STRINGi4932.YER040W

Structurei

3D structure databases

ProteinModelPortaliP18494
SMRiP18494
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi129 – 1379aaTAD9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi351 – 361Arg/Lys-rich (basic)Add BLAST11

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri306 – 330GATA-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000119221
HOGENOMiHOG000112739
InParanoidiP18494
KOiK09184
OMAiIQCFNCK
OrthoDBiEOG092C43RR

Family and domain databases

CDDicd00202 ZnF_GATA, 1 hit
Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 1 hit
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 1 hit
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 1 hit
PS50114 GATA_ZN_FINGER_2, 1 hit

Sequencei

Sequence statusi: Complete.

P18494-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA
60 70 80 90 100
FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT
110 120 130 140 150
NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS
160 170 180 190 200
GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN
210 220 230 240 250
NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD
260 270 280 290 300
SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK
310 320 330 340 350
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI
360 370 380 390 400
KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS
410 420 430 440 450
LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN
460 470 480 490 500
FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV
510 520 530 540 550
VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS
560 570 580 590 600
NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD
610 620 630 640 650
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR
660 670 680 690 700
PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG
710 720 730
GKISEDNSTK GSSKESSAIA DELDWLKFGI
Length:730
Mass (Da):79,383
Last modified:February 1, 1995 - v2
Checksum:i3159E1844469942E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti474P → G in AAA34645 (PubMed:1682800).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35267 Genomic DNA Translation: AAA34645.1
U18796 Genomic DNA Translation: AAB64575.1
BK006939 Genomic DNA Translation: DAA07694.1
PIRiS50543
RefSeqiNP_010958.3, NM_001178931.3

Genome annotation databases

EnsemblFungiiYER040W; YER040W; YER040W
GeneIDi856763
KEGGisce:YER040W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35267 Genomic DNA Translation: AAA34645.1
U18796 Genomic DNA Translation: AAB64575.1
BK006939 Genomic DNA Translation: DAA07694.1
PIRiS50543
RefSeqiNP_010958.3, NM_001178931.3

3D structure databases

ProteinModelPortaliP18494
SMRiP18494
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36776, 417 interactors
DIPiDIP-2353N
IntActiP18494, 11 interactors
MINTiP18494
STRINGi4932.YER040W

PTM databases

iPTMnetiP18494

Proteomic databases

MaxQBiP18494
PaxDbiP18494
PRIDEiP18494

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER040W; YER040W; YER040W
GeneIDi856763
KEGGisce:YER040W

Organism-specific databases

SGDiS000000842 GLN3

Phylogenomic databases

GeneTreeiENSGT00760000119221
HOGENOMiHOG000112739
InParanoidiP18494
KOiK09184
OMAiIQCFNCK
OrthoDBiEOG092C43RR

Enzyme and pathway databases

BioCyciYEAST:G3O-30221-MONOMER
ReactomeiR-SCE-5689880 Ub-specific processing proteases
R-SCE-9018519 Estrogen-dependent gene expression

Miscellaneous databases

PROiPR:P18494

Family and domain databases

CDDicd00202 ZnF_GATA, 1 hit
Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 1 hit
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 1 hit
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 1 hit
PS50114 GATA_ZN_FINGER_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLN3_YEAST
AccessioniPrimary (citable) accession number: P18494
Secondary accession number(s): D3DLU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
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Main funding by: National Institutes of Health

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