GLN3

Functionⁱ

Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	306 – 330	GATA-typePROSITE-ProRule annotationAdd BLAST		25

GO - Molecular functionⁱ

DNA-binding transcription factor activity
Source: SGD
Inferred from direct assayⁱ
- 8655543
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: SGD
Inferred from direct assayⁱ
- 7608102
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of transcription by RNA polymerase II
Source: SGD
Inferred from mutant phenotypeⁱ
- 26649943
nitrate assimilation Source: UniProtKB-KW
nitrogen catabolite activation of transcription from RNA polymerase II promoter
Source: SGD
Inferred from mutant phenotypeⁱ
- 7568152
positive regulation of transcription by RNA polymerase II
Source: SGD
Inferred from mutant phenotypeⁱ
- 2153652
positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation
Source: SGD
Inferred from mutant phenotypeⁱ
- 26649943
regulation of protein localization by the Cvt pathway
Source: SGD
Inferred from mutant phenotypeⁱ
- 26649943

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Nitrate assimilation, Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30221-MONOMER
Reactomeⁱ	R-SCE-5689880 Ub-specific processing proteases R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nitrogen regulatory protein GLN3
Gene namesⁱ	Name:GLN3 Ordered Locus Names:YER040W
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome V

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000000842 GLN3

SGDⁱ

S000000842 GLN3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000083477	1 – 730	Nitrogen regulatory protein GLN3Add BLAST		730

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	251	PhosphoserineCombined sources	1
Modified residueⁱ	267	PhosphoserineCombined sources	1
Modified residueⁱ	285	PhosphoserineCombined sources	1
Modified residueⁱ	469	PhosphoserineCombined sources	1
Modified residueⁱ	552	PhosphoserineCombined sources	1
Modified residueⁱ	562	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P18494
PaxDbⁱ	P18494
PRIDEⁱ	P18494

PTM databases

iPTMnetⁱ	P18494

iPTMnetⁱ

P18494

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
URE2	P23202	2	EBI-7657,EBI-20138

With

Entry

#Exp.

IntAct

Notes

URE2

P23202

2

EBI-7657,EBI-20138

Protein-protein interaction databases

BioGridⁱ	36776, 417 interactors
Database of interacting proteins More...DIPⁱ	DIP-2353N
IntActⁱ	P18494, 11 interactors
Molecular INTeraction database More...MINTⁱ	P18494
STRINGⁱ	4932.YER040W

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P18494
SMRⁱ	P18494
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	129 – 137	9aaTAD		9

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	351 – 361	Arg/Lys-rich (basic)Add BLAST		11

Domainⁱ

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	306 – 330	GATA-typePROSITE-ProRule annotationAdd BLAST		25

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119221
HOGENOMⁱ	HOG000112739
InParanoidⁱ	P18494
KEGG Orthology (KO) More...KOⁱ	K09184
OMAⁱ	IQCFNCK
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C43RR

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00202 ZnF_GATA, 1 hit
Gene3Dⁱ	3.30.50.10, 1 hit
InterProⁱ	View protein in InterPro IPR039355 Transcription_factor_GATA IPR000679 Znf_GATA IPR013088 Znf_NHR/GATA
PANTHERⁱ	PTHR10071 PTHR10071, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00320 GATA, 1 hit
PRINTSⁱ	PR00619 GATAZNFINGER
SMARTⁱ	View protein in SMART SM00401 ZnF_GATA, 1 hit
PROSITEⁱ	View protein in PROSITE PS00344 GATA_ZN_FINGER_1, 1 hit PS50114 GATA_ZN_FINGER_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P18494-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA 
        60         70         80         90        100
FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT 
       110        120        130        140        150
NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS 
       160        170        180        190        200
GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN 
       210        220        230        240        250
NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD 
       260        270        280        290        300
SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK 
       310        320        330        340        350
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI 
       360        370        380        390        400
KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS 
       410        420        430        440        450
LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN 
       460        470        480        490        500
FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV 
       510        520        530        540        550
VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS 
       560        570        580        590        600
NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD 
       610        620        630        640        650
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR 
       660        670        680        690        700
PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG 
       710        720        730
GKISEDNSTK GSSKESSAIA DELDWLKFGI

Length:730

Mass (Da):79,383

Last modified:February 1, 1995 - v2

Checksum:ⁱ3159E1844469942E

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	474	P → G in AAA34645 (PubMed:1682800).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M35267 Genomic DNA Translation: AAA34645.1 U18796 Genomic DNA Translation: AAB64575.1 BK006939 Genomic DNA Translation: DAA07694.1
PIRⁱ	S50543
NCBI Reference Sequences More...RefSeqⁱ	NP_010958.3, NM_001178931.3

Genome annotation databases

EnsemblFungiⁱ	YER040W; YER040W; YER040W
GeneIDⁱ	856763
KEGGⁱ	sce:YER040W

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P18494	Transcriptional activator of nitrogen-regulated genes		YEAS1		730	UniRef90_P18494
K7_Gln3p		YEASK		730
Gln3p		YEAS8		730
GLN3p Transcriptional activator		Saccharomyces sp. 'boulardii'		730
Gln3p		YEASL		730
+9

Protein	Similar proteins		Species	Score	Length	Source
P18494	Nitrogen-responsive transcriptional regulator		YEASX		730	UniRef50_P18494
Gln3p		YEAS2		730
Gln3p		YEASC		730
Gln3p		SACCK		730
Transcriptional activator of nitrogen-regulated genes		YEAS1		730
+13

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M35267 Genomic DNA Translation: AAA34645.1 U18796 Genomic DNA Translation: AAB64575.1 BK006939 Genomic DNA Translation: DAA07694.1
PIRⁱ	S50543
RefSeqⁱ	NP_010958.3, NM_001178931.3

3D structure databases

ProteinModelPortalⁱ	P18494
SMRⁱ	P18494
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	36776, 417 interactors
DIPⁱ	DIP-2353N
IntActⁱ	P18494, 11 interactors
MINTⁱ	P18494
STRINGⁱ	4932.YER040W

PTM databases

iPTMnetⁱ	P18494

iPTMnetⁱ

P18494

Proteomic databases

MaxQBⁱ	P18494
PaxDbⁱ	P18494
PRIDEⁱ	P18494

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YER040W; YER040W; YER040W
GeneIDⁱ	856763
KEGGⁱ	sce:YER040W

Organism-specific databases

SGDⁱ	S000000842 GLN3

Phylogenomic databases

GeneTreeⁱ	ENSGT00760000119221
HOGENOMⁱ	HOG000112739
InParanoidⁱ	P18494
KOⁱ	K09184
OMAⁱ	IQCFNCK
OrthoDBⁱ	EOG092C43RR

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30221-MONOMER
Reactomeⁱ	R-SCE-5689880 Ub-specific processing proteases R-SCE-9018519 Estrogen-dependent gene expression

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P18494

PROⁱ

PR:P18494

Family and domain databases

CDDⁱ	cd00202 ZnF_GATA, 1 hit
Gene3Dⁱ	3.30.50.10, 1 hit
InterProⁱ	View protein in InterPro IPR039355 Transcription_factor_GATA IPR000679 Znf_GATA IPR013088 Znf_NHR/GATA
PANTHERⁱ	PTHR10071 PTHR10071, 1 hit
Pfamⁱ	View protein in Pfam PF00320 GATA, 1 hit
PRINTSⁱ	PR00619 GATAZNFINGER
SMARTⁱ	View protein in SMART SM00401 ZnF_GATA, 1 hit
PROSITEⁱ	View protein in PROSITE PS00344 GATA_ZN_FINGER_1, 1 hit PS50114 GATA_ZN_FINGER_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLN3_YEAST
Accessionⁱ	P18494Primary (citable) accession number: P18494 Secondary accession number(s): D3DLU0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
	Last sequence update:	February 1, 1995
	Last modified:	November 7, 2018
	This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P18494 (GLN3_YEAST)

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Nitrogen regulatory protein GLN3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytosol

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Compositional bias

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ