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Protein

Calreticulin

Gene

Calr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.By similarity

Caution

Was originally (Ref. 9) thought to be D-beta-hydroxybutyrate dehydrogenase.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Calcium; via carbonyl oxygenBy similarity1
Metal bindingi62Calcium; via carbonyl oxygenBy similarity1
Metal bindingi64Calcium; via carbonyl oxygenBy similarity1
Binding sitei109CarbohydrateBy similarity1
Binding sitei111CarbohydrateBy similarity1
Binding sitei128CarbohydrateBy similarity1
Binding sitei135CarbohydrateBy similarity1
Binding sitei317CarbohydrateBy similarity1
Metal bindingi328CalciumBy similarity1

GO - Molecular functioni

  • androgen receptor binding Source: Ensembl
  • calcium ion binding Source: RGD
  • carbohydrate binding Source: UniProtKB-KW
  • hormone binding Source: RGD
  • integrin binding Source: Ensembl
  • iron ion binding Source: RGD
  • mRNA binding Source: BHF-UCL
  • peptide binding Source: RGD
  • ubiquitin protein ligase binding Source: Ensembl
  • unfolded protein binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandCalcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-1236974 ER-Phagosome pathway
R-RNO-3000480 Scavenging by Class A Receptors
R-RNO-901042 Calnexin/calreticulin cycle
R-RNO-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Protein family/group databases

UniLectiniP18418

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Alternative name(s):
CALBP
CRP55
Calcium-binding protein 3
Short name:
CABP3
Calregulin
Endoplasmic reticulum resident protein 60
Short name:
ERp60
HACBP
Gene namesi
Name:Calr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620288 Calr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 173 PublicationsAdd BLAST17
ChainiPRO_000000417718 – 416CalreticulinAdd BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysineBy similarity1
Disulfide bondi105 ↔ 137By similarity
Modified residuei159N6-acetyllysineBy similarity1
Modified residuei209N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP18418
PRIDEiP18418

2D gel databases

World-2DPAGEi0004:P18418

PTM databases

iPTMnetiP18418
PhosphoSitePlusiP18418
SwissPalmiP18418

Expressioni

Tissue specificityi

Predentin and odontoblast.1 Publication

Gene expression databases

BgeeiENSRNOG00000003029
GenevisibleiP18418 RN

Interactioni

Subunit structurei

Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with GABARAP, NR3C1 and TRIM21. Interacts with PPIB and SPACA9. Interacts (via P-domain) with PDIA5 (By similarity). Interacts with PDIA3/ERp57 (PubMed:11842220).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PDIA3P301012EBI-916742,EBI-979862From Homo sapiens.

GO - Molecular functioni

  • androgen receptor binding Source: Ensembl
  • integrin binding Source: Ensembl
  • ubiquitin protein ligase binding Source: Ensembl
  • unfolded protein binding Source: RGD

Protein-protein interaction databases

BioGridi249008, 3 interactors
CORUMiP18418
IntActiP18418, 6 interactors
MINTiP18418
STRINGi10116.ENSRNOP00000004091

Structurei

Secondary structure

1416
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni220 – 222Combined sources3
Beta strandi223 – 225Combined sources3
Turni234 – 236Combined sources3
Beta strandi240 – 243Combined sources4
Helixi255 – 258Combined sources4
Beta strandi265 – 267Combined sources3
Beta strandi301 – 303Combined sources3

3D structure databases

ProteinModelPortaliP18418
SMRiP18418
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18418

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati191 – 2021-1Add BLAST12
Repeati210 – 2211-2Add BLAST12
Repeati227 – 2381-3Add BLAST12
Repeati244 – 2551-4Add BLAST12
Repeati259 – 2692-1Add BLAST11
Repeati273 – 2832-2Add BLAST11
Repeati287 – 2972-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 197N-domainAdd BLAST180
Regioni191 – 2554 X approximate repeatsAdd BLAST65
Regioni198 – 308P-domainAdd BLAST111
Regioni237 – 270Interaction with PPIBBy similarityAdd BLAST34
Regioni259 – 2973 X approximate repeatsAdd BLAST39
Regioni309 – 416C-domainAdd BLAST108

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi413 – 416Prevents secretion from ER4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi351 – 407Asp/Glu/Lys-richAdd BLAST57

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674 Eukaryota
ENOG410XRR7 LUCA
GeneTreeiENSGT00430000030841
HOGENOMiHOG000192435
HOVERGENiHBG005407
InParanoidiP18418
KOiK08057
OMAiDTWIYSK
OrthoDBiEOG091G0AP1
PhylomeDBiP18418
TreeFamiTF338438

Family and domain databases

Gene3Di2.10.250.10, 2 hits
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009169 Calreticulin
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 2 hits
PIRSFiPIRSF002356 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 2 hits
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
PS00805 CALRETICULIN_REPEAT, 3 hits
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSVPLLLG LLGLAAADPA IYFKEQFLDG DAWTNRWVES KHKSDFGKFV
60 70 80 90 100
LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE
110 120 130 140 150
QNIDCGGGYV KLFPGGLDQK DMHGDSEYNI MFGPDICGPG TKKVHVIFNY
160 170 180 190 200
KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW
210 220 230 240 250
DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP
260 270 280 290 300
EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS
310 320 330 340 350
PDANIYAYDS FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT
360 370 380 390 400
KAAEKQMKDK QDEEQRLKEE EEDKKRKEEE EAEDKEDEDD RDEDEDEEDE
410
KEEDEEDATG QAKDEL
Length:416
Mass (Da):47,995
Last modified:November 1, 1990 - v1
Checksum:i2E6713CED31A2970
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti269P → R in CAA31987 (Ref. 9) Curated1
Sequence conflicti359 – 361DKQ → AAG in CAA31987 (Ref. 9) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53363 mRNA Translation: CAA37446.1
D78308 mRNA Translation: BAA11345.1
X79327 mRNA Translation: CAA55890.1
BC062395 mRNA Translation: AAH62395.1
X13702 mRNA Translation: CAA31987.1
PIRiJH0819
RefSeqiNP_071794.1, NM_022399.2
UniGeneiRn.974

Genome annotation databases

EnsembliENSRNOT00000004091; ENSRNOP00000004091; ENSRNOG00000003029
GeneIDi64202
KEGGirno:64202

Similar proteinsi

Entry informationi

Entry nameiCALR_RAT
AccessioniPrimary (citable) accession number: P18418
Secondary accession number(s): P10452
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 18, 2018
This is version 180 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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