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Protein

Acetylornithine/succinyldiaminopimelate aminotransferase

Gene

argD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in both the arginine and lysine biosynthetic pathways.UniRule annotation1 Publication

Miscellaneous

The reaction catalyzed by ACOAT is highly reversible. This enzyme may also transaminate ornithine.

Catalytic activityi

N2-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.UniRule annotation1 Publication
N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate.UniRule annotation1 Publication

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 PublicationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation1 Publication

Kineticsi

  1. KM=0.15 mM for N-acetylornithine1 Publication
  2. KM=0.075 mM for N-succinyldiaminopimelate1 Publication

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation1 Publication
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. Amino-acid acetyltransferase (argA)
    2. Acetylglutamate kinase (argB)
    3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
    4. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Pathwayi: L-lysine biosynthesis via DAP pathway

    This protein is involved in step 2 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).UniRule annotation1 Publication
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
    2. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
    3. Succinyl-diaminopimelate desuccinylase (dapE)
    This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei141Pyridoxal phosphate; via carbonyl oxygenUniRule annotationBy similarity1
    Binding sitei144N2-acetyl-L-ornithineUniRule annotationBy similarity1
    Binding sitei283N2-acetyl-L-ornithineUniRule annotationBy similarity1
    Binding sitei284Pyridoxal phosphateUniRule annotationBy similarity1

    GO - Molecular functioni

    • N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Source: EcoliWiki
    • pyridoxal phosphate binding Source: GO_Central
    • succinyldiaminopimelate transaminase activity Source: EcoliWiki

    GO - Biological processi

    Keywordsi

    Molecular functionAminotransferase, Transferase
    Biological processAmino-acid biosynthesis, Arginine biosynthesis, Lysine biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:ACETYLORNTRANSAM-MONOMER
    MetaCyc:ACETYLORNTRANSAM-MONOMER
    UniPathwayi
    UPA00034;UER00020

    UPA00068;UER00109

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetylornithine/succinyldiaminopimelate aminotransferaseUniRule annotationCurated (EC:2.6.1.11UniRule annotation1 Publication, EC:2.6.1.17UniRule annotation1 Publication)
    Short name:
    ACOAT1 PublicationUniRule annotation
    Short name:
    DapATase1 PublicationUniRule annotation
    Short name:
    Succinyldiaminopimelate transferaseUniRule annotation
    Gene namesi
    Name:argDUniRule annotation
    Synonyms:dapCUniRule annotation, dtu
    Ordered Locus Names:b3359, JW3322
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10066 argD

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00001127432 – 406Acetylornithine/succinyldiaminopimelate aminotransferaseAdd BLAST405

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei255N6-(pyridoxal phosphate)lysineUniRule annotationBy similarity1

    Proteomic databases

    EPDiP18335
    PaxDbiP18335
    PRIDEiP18335

    2D gel databases

    SWISS-2DPAGEiP18335

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    BioGridi4260742, 7 interactors
    DIPiDIP-9138N
    IntActiP18335, 6 interactors
    STRINGi316385.ECDH10B_3535

    Structurei

    3D structure databases

    ProteinModelPortaliP18335
    SMRiP18335
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni108 – 109Pyridoxal phosphate bindingUniRule annotationBy similarity2
    Regioni226 – 229Pyridoxal phosphate bindingUniRule annotationBy similarity4

    Sequence similaritiesi

    Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4105C8Y Bacteria
    COG4992 LUCA
    HOGENOMiHOG000020206
    InParanoidiP18335
    KOiK00821
    PhylomeDBiP18335

    Family and domain databases

    CDDicd00610 OAT_like, 1 hit
    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    HAMAPiMF_01107 ArgD_aminotrans_3, 1 hit
    InterProiView protein in InterPro
    IPR017652 Ac/SucOrn_transaminase_bac
    IPR004636 AcOrn/SuccOrn_fam
    IPR005814 Aminotrans_3
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PfamiView protein in Pfam
    PF00202 Aminotran_3, 1 hit
    PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR03246 arg_catab_astC, 1 hit
    TIGR00707 argD, 1 hit
    PROSITEiView protein in PROSITE
    PS00600 AA_TRANSFER_CLASS_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18335-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAIEQTAITR ATFDEVILPI YAPAEFIPVK GQGSRIWDQQ GKEYVDFAGG
    60 70 80 90 100
    IAVTALGHCH PALVNALKTQ GETLWHISNV FTNEPALRLG RKLIEATFAE
    110 120 130 140 150
    RVVFMNSGTE ANETAFKLAR HYACVRHSPF KTKIIAFHNA FHGRSLFTVS
    160 170 180 190 200
    VGGQPKYSDG FGPKPADIIH VPFNDLHAVK AVMDDHTCAV VVEPIQGEGG
    210 220 230 240 250
    VTAATPEFLQ GLRELCDQHQ ALLVFDEVQC GMGRTGDLFA YMHYGVTPDI
    260 270 280 290 300
    LTSAKALGGG FPISAMLTTA EIASAFHPGS HGSTYGGNPL ACAVAGAAFD
    310 320 330 340 350
    IINTPEVLEG IQAKRQRFVD HLQKIDQQYD VFSDIRGMGL LIGAELKPQY
    360 370 380 390 400
    KGRARDFLYA GAEAGVMVLN AGPDVMRFAP SLVVEDADID EGMQRFAHAV

    AKVVGA
    Length:406
    Mass (Da):43,767
    Last modified:January 23, 2007 - v4
    Checksum:i645AA1EBCA442214
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti245 – 247GVT → ALA in AAA23480 (PubMed:2199330).Curated3

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32796 Genomic DNA Translation: AAA23480.1
    U18997 Genomic DNA Translation: AAA58156.1
    U00096 Genomic DNA Translation: AAC76384.1
    AP009048 Genomic DNA Translation: BAE77931.1
    PIRiB65130
    RefSeqiNP_417818.1, NC_000913.3
    WP_000963792.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76384; AAC76384; b3359
    BAE77931; BAE77931; BAE77931
    GeneIDi947864
    KEGGiecj:JW3322
    eco:b3359
    PATRICifig|1411691.4.peg.3371

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32796 Genomic DNA Translation: AAA23480.1
    U18997 Genomic DNA Translation: AAA58156.1
    U00096 Genomic DNA Translation: AAC76384.1
    AP009048 Genomic DNA Translation: BAE77931.1
    PIRiB65130
    RefSeqiNP_417818.1, NC_000913.3
    WP_000963792.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP18335
    SMRiP18335
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260742, 7 interactors
    DIPiDIP-9138N
    IntActiP18335, 6 interactors
    STRINGi316385.ECDH10B_3535

    2D gel databases

    SWISS-2DPAGEiP18335

    Proteomic databases

    EPDiP18335
    PaxDbiP18335
    PRIDEiP18335

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76384; AAC76384; b3359
    BAE77931; BAE77931; BAE77931
    GeneIDi947864
    KEGGiecj:JW3322
    eco:b3359
    PATRICifig|1411691.4.peg.3371

    Organism-specific databases

    EchoBASEiEB0064
    EcoGeneiEG10066 argD

    Phylogenomic databases

    eggNOGiENOG4105C8Y Bacteria
    COG4992 LUCA
    HOGENOMiHOG000020206
    InParanoidiP18335
    KOiK00821
    PhylomeDBiP18335

    Enzyme and pathway databases

    UniPathwayi
    UPA00034;UER00020

    UPA00068;UER00109

    BioCyciEcoCyc:ACETYLORNTRANSAM-MONOMER
    MetaCyc:ACETYLORNTRANSAM-MONOMER

    Miscellaneous databases

    PROiPR:P18335

    Family and domain databases

    CDDicd00610 OAT_like, 1 hit
    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    HAMAPiMF_01107 ArgD_aminotrans_3, 1 hit
    InterProiView protein in InterPro
    IPR017652 Ac/SucOrn_transaminase_bac
    IPR004636 AcOrn/SuccOrn_fam
    IPR005814 Aminotrans_3
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PfamiView protein in Pfam
    PF00202 Aminotran_3, 1 hit
    PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR03246 arg_catab_astC, 1 hit
    TIGR00707 argD, 1 hit
    PROSITEiView protein in PROSITE
    PS00600 AA_TRANSFER_CLASS_3, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiARGD_ECOLI
    AccessioniPrimary (citable) accession number: P18335
    Secondary accession number(s): Q2M725
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: November 7, 2018
    This is version 160 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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