argD

Functionⁱ

Involved in both the arginine and lysine biosynthetic pathways.UniRule annotation1 Publication

Miscellaneous

The reaction catalyzed by ACOAT is highly reversible. This enzyme may also transaminate ornithine.

Catalytic activityⁱ

N₂-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.UniRule annotation1 Publication

N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate.UniRule annotation1 Publication

Cofactorⁱ

pyridoxal 5'-phosphateUniRule annotation1 PublicationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation1 Publication

Kineticsⁱ

Pathwayⁱ: L-arginine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation1 Publication
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

Amino-acid acetyltransferase (argA)
Acetylglutamate kinase (argB)
N-acetyl-gamma-glutamyl-phosphate reductase (argC)
Acetylornithine/succinyldiaminopimelate aminotransferase (argD)

This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayⁱ: L-lysine biosynthesis via DAP pathway

This protein is involved in step 2 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).UniRule annotation1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:

2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
Succinyl-diaminopimelate desuccinylase (dapE)

This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	141	Pyridoxal phosphate; via carbonyl oxygenUniRule annotationBy similarity	1
Binding siteⁱ	144	N2-acetyl-L-ornithineUniRule annotationBy similarity	1
Binding siteⁱ	283	N2-acetyl-L-ornithineUniRule annotationBy similarity	1
Binding siteⁱ	284	Pyridoxal phosphateUniRule annotationBy similarity	1

GO - Molecular functionⁱ

N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 10074354
pyridoxal phosphate binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
succinyldiaminopimelate transaminase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 10074354

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminotransferase, Transferase
Biological process	Amino-acid biosynthesis, Arginine biosynthesis, Lysine biosynthesis
Ligand	Pyridoxal phosphate

Enzyme and pathway databases

BioCycⁱ	EcoCyc:ACETYLORNTRANSAM-MONOMER MetaCyc:ACETYLORNTRANSAM-MONOMER
UniPathwayⁱ	UPA00034;UER00020 UPA00068;UER00109

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Acetylornithine/succinyldiaminopimelate aminotransferaseUniRule annotationCurated (EC:2.6.1.11UniRule annotation1 Publication, EC:2.6.1.17UniRule annotation1 Publication) Short name: ACOAT1 PublicationUniRule annotation Short name: DapATase1 PublicationUniRule annotation Short name: Succinyldiaminopimelate transferaseUniRule annotation
Gene namesⁱ	Name:argDUniRule annotation Synonyms:dapCUniRule annotation, dtu Ordered Locus Names:b3359, JW3322
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10066 argD

EcoGeneⁱ

EG10066 argD

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01107
Curated

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed2 Publications
ChainⁱPRO_0000112743	2 – 406	Acetylornithine/succinyldiaminopimelate aminotransferaseAdd BLAST		405

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	255	N6-(pyridoxal phosphate)lysineUniRule annotationBy similarity		1

Proteomic databases

EPDⁱ	P18335
PaxDbⁱ	P18335
PRIDEⁱ	P18335

2D gel databases

SWISS-2DPAGEⁱ	P18335

SWISS-2DPAGEⁱ

P18335

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridⁱ	4260742, 7 interactors
Database of interacting proteins More...DIPⁱ	DIP-9138N
IntActⁱ	P18335, 6 interactors
STRINGⁱ	316385.ECDH10B_3535

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P18335
SMRⁱ	P18335
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	108 – 109	Pyridoxal phosphate bindingUniRule annotationBy similarity		2
Regionⁱ	226 – 229	Pyridoxal phosphate bindingUniRule annotationBy similarity		4

Sequence similaritiesⁱ

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGⁱ	ENOG4105C8Y Bacteria COG4992 LUCA
HOGENOMⁱ	HOG000020206
InParanoidⁱ	P18335
KEGG Orthology (KO) More...KOⁱ	K00821
PhylomeDBⁱ	P18335

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00610 OAT_like, 1 hit
Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 1 hit
HAMAPⁱ	MF_01107 ArgD_aminotrans_3, 1 hit
InterProⁱ	View protein in InterPro IPR017652 Ac/SucOrn_transaminase_bac IPR004636 AcOrn/SuccOrn_fam IPR005814 Aminotrans_3 IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00202 Aminotran_3, 1 hit
PIRSFⁱ	PIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
TIGRFAMsⁱ	TIGR03246 arg_catab_astC, 1 hit TIGR00707 argD, 1 hit
PROSITEⁱ	View protein in PROSITE PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P18335-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAIEQTAITR ATFDEVILPI YAPAEFIPVK GQGSRIWDQQ GKEYVDFAGG 
        60         70         80         90        100
IAVTALGHCH PALVNALKTQ GETLWHISNV FTNEPALRLG RKLIEATFAE 
       110        120        130        140        150
RVVFMNSGTE ANETAFKLAR HYACVRHSPF KTKIIAFHNA FHGRSLFTVS 
       160        170        180        190        200
VGGQPKYSDG FGPKPADIIH VPFNDLHAVK AVMDDHTCAV VVEPIQGEGG 
       210        220        230        240        250
VTAATPEFLQ GLRELCDQHQ ALLVFDEVQC GMGRTGDLFA YMHYGVTPDI 
       260        270        280        290        300
LTSAKALGGG FPISAMLTTA EIASAFHPGS HGSTYGGNPL ACAVAGAAFD 
       310        320        330        340        350
IINTPEVLEG IQAKRQRFVD HLQKIDQQYD VFSDIRGMGL LIGAELKPQY 
       360        370        380        390        400
KGRARDFLYA GAEAGVMVLN AGPDVMRFAP SLVVEDADID EGMQRFAHAV 

AKVVGA

Length:406

Mass (Da):43,767

Last modified:January 23, 2007 - v4

Checksum:ⁱ645AA1EBCA442214

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	245 – 247	GVT → ALA in AAA23480 (PubMed:2199330).Curated		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M32796 Genomic DNA Translation: AAA23480.1 U18997 Genomic DNA Translation: AAA58156.1 U00096 Genomic DNA Translation: AAC76384.1 AP009048 Genomic DNA Translation: BAE77931.1
PIRⁱ	B65130
NCBI Reference Sequences More...RefSeqⁱ	NP_417818.1, NC_000913.3 WP_000963792.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76384; AAC76384; b3359 BAE77931; BAE77931; BAE77931
GeneIDⁱ	947864
KEGGⁱ	ecj:JW3322 eco:b3359
PATRICⁱ	fig\|1411691.4.peg.3371

Protein	Similar proteins		Species	Score	Length	Source
P18335	Aspartate aminotransferase family protein		Shigella sp.		406	UniRef100_P18335
Acetylornithine/succinyldiaminopimelate aminotransferase		Klebsiella pneumoniae IS22		406
Acetylornithine/succinyldiaminopimelate aminotransferase		ECOLX		406
Acetylornithine/succinyldiaminopimelate aminotransferase		Escherichia coli G3/10		406
Acetylornithine/succinyldiaminopimelate aminotransferase		Escherichia coli KTE108		406
+42

Protein	Similar proteins		Species	Score	Length	Source
P18335	Acetylornithine/succinyldiaminopimelate aminotransferase	)	SALTY		405	UniRef90_P40732
Acetylornithine/succinyldiaminopimelate aminotransferase		ECO57		406
Acetylornithine/succinyldiaminopimelate aminotransferase		ECOL6		406
Acetylornithine/succinyldiaminopimelate aminotransferase		SHIFL		406
Acetylornithine/succinyldiaminopimelate aminotransferase		SALTI		405
+1009

Protein	Similar proteins		Species	Score	Length	Source
P18335	Acetylornithine/succinyldiaminopimelate aminotransferase	)	SALTY		405	UniRef50_P40732
Acetylornithine/succinyldiaminopimelate aminotransferase		SHIFL		406
Acetylornithine/succinyldiaminopimelate aminotransferase		ECO57		406
Acetylornithine/succinyldiaminopimelate aminotransferase		ECOL6		406
Acetylornithine/succinyldiaminopimelate aminotransferase		SALTI		405
+10661

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M32796 Genomic DNA Translation: AAA23480.1 U18997 Genomic DNA Translation: AAA58156.1 U00096 Genomic DNA Translation: AAC76384.1 AP009048 Genomic DNA Translation: BAE77931.1
PIRⁱ	B65130
RefSeqⁱ	NP_417818.1, NC_000913.3 WP_000963792.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P18335
SMRⁱ	P18335
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260742, 7 interactors
DIPⁱ	DIP-9138N
IntActⁱ	P18335, 6 interactors
STRINGⁱ	316385.ECDH10B_3535

2D gel databases

SWISS-2DPAGEⁱ	P18335

SWISS-2DPAGEⁱ

P18335

Proteomic databases

EPDⁱ	P18335
PaxDbⁱ	P18335
PRIDEⁱ	P18335

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76384; AAC76384; b3359 BAE77931; BAE77931; BAE77931
GeneIDⁱ	947864
KEGGⁱ	ecj:JW3322 eco:b3359
PATRICⁱ	fig\|1411691.4.peg.3371

Organism-specific databases

EchoBASEⁱ	EB0064
EcoGeneⁱ	EG10066 argD

Phylogenomic databases

eggNOGⁱ	ENOG4105C8Y Bacteria COG4992 LUCA
HOGENOMⁱ	HOG000020206
InParanoidⁱ	P18335
KOⁱ	K00821
PhylomeDBⁱ	P18335

Enzyme and pathway databases

UniPathwayⁱ	UPA00034;UER00020 UPA00068;UER00109
BioCycⁱ	EcoCyc:ACETYLORNTRANSAM-MONOMER MetaCyc:ACETYLORNTRANSAM-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P18335

PROⁱ

PR:P18335

Family and domain databases

CDDⁱ	cd00610 OAT_like, 1 hit
Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 1 hit
HAMAPⁱ	MF_01107 ArgD_aminotrans_3, 1 hit
InterProⁱ	View protein in InterPro IPR017652 Ac/SucOrn_transaminase_bac IPR004636 AcOrn/SuccOrn_fam IPR005814 Aminotrans_3 IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
Pfamⁱ	View protein in Pfam PF00202 Aminotran_3, 1 hit
PIRSFⁱ	PIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
TIGRFAMsⁱ	TIGR03246 arg_catab_astC, 1 hit TIGR00707 argD, 1 hit
PROSITEⁱ	View protein in PROSITE PS00600 AA_TRANSFER_CLASS_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ARGD_ECOLI
Accessionⁱ	P18335Primary (citable) accession number: P18335 Secondary accession number(s): Q2M725
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 160 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P18335 (ARGD_ECOLI)

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Acetylornithine/succinyldiaminopimelate aminotransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-arginine biosynthesis

Pathwayi: L-lysine biosynthesis via DAP pathway

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

Pathwayⁱ: L-arginine biosynthesis

Pathwayⁱ: L-lysine biosynthesis via DAP pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ