UniProtKB - P18335 (ARGD_ECOLI)
Protein
Acetylornithine/succinyldiaminopimelate aminotransferase
Gene
argD
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in both the arginine and lysine biosynthetic pathways.UniRule annotation1 Publication
Miscellaneous
The reaction catalyzed by ACOAT is highly reversible. This enzyme may also transaminate ornithine.
Catalytic activityi
- 2-oxoglutarate + N2-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehydeUniRule annotation1 PublicationEC:2.6.1.11UniRule annotation1 Publication
- 2-oxoglutarate + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (S)-2-succinylamino-6-oxoheptanedioate + L-glutamateUniRule annotation1 PublicationEC:2.6.1.17UniRule annotation1 Publication
Cofactori
pyridoxal 5'-phosphateUniRule annotation1 PublicationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation1 Publication
Kineticsi
- KM=0.15 mM for N-acetylornithine1 Publication
- KM=0.075 mM for N-succinyldiaminopimelate1 Publication
: L-arginine biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation1 PublicationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Amino-acid acetyltransferase (argA), Amino-acid acetyltransferase (argA)
- Acetylglutamate kinase (argB), Acetylglutamate kinase (argB)
- N-acetyl-gamma-glutamyl-phosphate reductase (argC), N-acetyl-gamma-glutamyl-phosphate reductase (argC)
- Acetylornithine/succinyldiaminopimelate aminotransferase (argD), Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-lysine biosynthesis via DAP pathway
This protein is involved in step 2 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).UniRule annotation1 PublicationProteins known to be involved in the 3 steps of the subpathway in this organism are:
- 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD), 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
- Acetylornithine/succinyldiaminopimelate aminotransferase (argD), Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
- Succinyl-diaminopimelate desuccinylase (dapE), Succinyl-diaminopimelate desuccinylase (dapE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 141 | Pyridoxal phosphate; via carbonyl oxygenUniRule annotationBy similarity | 1 | |
Binding sitei | 144 | N2-acetyl-L-ornithineUniRule annotationBy similarity | 1 | |
Binding sitei | 283 | N2-acetyl-L-ornithineUniRule annotationBy similarity | 1 | |
Binding sitei | 284 | Pyridoxal phosphateUniRule annotationBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: GO_Central
- N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Source: EcoliWiki
- pyridoxal phosphate binding Source: EcoCyc
- succinyldiaminopimelate transaminase activity Source: EcoCyc
GO - Biological processi
- arginine biosynthetic process via ornithine Source: EcoCyc
- lysine biosynthetic process via diaminopimelate and N-succinyl-2-amino-6-ketopimelate Source: EcoCyc
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis, Lysine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:ACETYLORNTRANSAM-MONOMER MetaCyc:ACETYLORNTRANSAM-MONOMER |
UniPathwayi | UPA00034;UER00020 UPA00068;UER00109 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylornithine/succinyldiaminopimelate aminotransferaseUniRule annotationCurated (EC:2.6.1.11UniRule annotation1 Publication, EC:2.6.1.17UniRule annotation1 Publication)Short name: ACOAT1 PublicationUniRule annotation Short name: DapATase1 PublicationUniRule annotation Short name: Succinyldiaminopimelate transferaseUniRule annotation |
Gene namesi | Name:argDUniRule annotation Synonyms:dapCUniRule annotation, dtu Ordered Locus Names:b3359, JW3322 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotationCurated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000112743 | 2 – 406 | Acetylornithine/succinyldiaminopimelate aminotransferaseAdd BLAST | 405 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 255 | N6-(pyridoxal phosphate)lysineUniRule annotationBy similarity | 1 |
Proteomic databases
jPOSTi | P18335 |
PaxDbi | P18335 |
PRIDEi | P18335 |
2D gel databases
SWISS-2DPAGEi | P18335 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 4260742, 7 interactors |
DIPi | DIP-9138N |
IntActi | P18335, 6 interactors |
STRINGi | 511145.b3359 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 108 – 109 | Pyridoxal phosphate bindingUniRule annotationBy similarity | 2 | |
Regioni | 226 – 229 | Pyridoxal phosphate bindingUniRule annotationBy similarity | 4 |
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotationCurated
Phylogenomic databases
eggNOGi | COG4992, Bacteria |
HOGENOMi | CLU_016922_10_1_6 |
InParanoidi | P18335 |
PhylomeDBi | P18335 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01107, ArgD_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR017652, Ac/SucOrn_transaminase_bac IPR004636, AcOrn/SuccOrn_fam IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR03246, arg_catab_astC, 1 hit TIGR00707, argD, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P18335-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIEQTAITR ATFDEVILPI YAPAEFIPVK GQGSRIWDQQ GKEYVDFAGG
60 70 80 90 100
IAVTALGHCH PALVNALKTQ GETLWHISNV FTNEPALRLG RKLIEATFAE
110 120 130 140 150
RVVFMNSGTE ANETAFKLAR HYACVRHSPF KTKIIAFHNA FHGRSLFTVS
160 170 180 190 200
VGGQPKYSDG FGPKPADIIH VPFNDLHAVK AVMDDHTCAV VVEPIQGEGG
210 220 230 240 250
VTAATPEFLQ GLRELCDQHQ ALLVFDEVQC GMGRTGDLFA YMHYGVTPDI
260 270 280 290 300
LTSAKALGGG FPISAMLTTA EIASAFHPGS HGSTYGGNPL ACAVAGAAFD
310 320 330 340 350
IINTPEVLEG IQAKRQRFVD HLQKIDQQYD VFSDIRGMGL LIGAELKPQY
360 370 380 390 400
KGRARDFLYA GAEAGVMVLN AGPDVMRFAP SLVVEDADID EGMQRFAHAV
AKVVGA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 245 – 247 | GVT → ALA in AAA23480 (PubMed:2199330).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32796 Genomic DNA Translation: AAA23480.1 U18997 Genomic DNA Translation: AAA58156.1 U00096 Genomic DNA Translation: AAC76384.1 AP009048 Genomic DNA Translation: BAE77931.1 |
PIRi | B65130 |
RefSeqi | NP_417818.1, NC_000913.3 WP_000963792.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76384; AAC76384; b3359 BAE77931; BAE77931; BAE77931 |
GeneIDi | 58460184 947864 |
KEGGi | ecj:JW3322 eco:b3359 |
PATRICi | fig|1411691.4.peg.3371 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32796 Genomic DNA Translation: AAA23480.1 U18997 Genomic DNA Translation: AAA58156.1 U00096 Genomic DNA Translation: AAC76384.1 AP009048 Genomic DNA Translation: BAE77931.1 |
PIRi | B65130 |
RefSeqi | NP_417818.1, NC_000913.3 WP_000963792.1, NZ_SSZK01000008.1 |
3D structure databases
SMRi | P18335 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260742, 7 interactors |
DIPi | DIP-9138N |
IntActi | P18335, 6 interactors |
STRINGi | 511145.b3359 |
2D gel databases
SWISS-2DPAGEi | P18335 |
Proteomic databases
jPOSTi | P18335 |
PaxDbi | P18335 |
PRIDEi | P18335 |
Genome annotation databases
EnsemblBacteriai | AAC76384; AAC76384; b3359 BAE77931; BAE77931; BAE77931 |
GeneIDi | 58460184 947864 |
KEGGi | ecj:JW3322 eco:b3359 |
PATRICi | fig|1411691.4.peg.3371 |
Organism-specific databases
EchoBASEi | EB0064 |
Phylogenomic databases
eggNOGi | COG4992, Bacteria |
HOGENOMi | CLU_016922_10_1_6 |
InParanoidi | P18335 |
PhylomeDBi | P18335 |
Enzyme and pathway databases
UniPathwayi | UPA00034;UER00020 UPA00068;UER00109 |
BioCyci | EcoCyc:ACETYLORNTRANSAM-MONOMER MetaCyc:ACETYLORNTRANSAM-MONOMER |
Miscellaneous databases
PROi | PR:P18335 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01107, ArgD_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR017652, Ac/SucOrn_transaminase_bac IPR004636, AcOrn/SuccOrn_fam IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR03246, arg_catab_astC, 1 hit TIGR00707, argD, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARGD_ECOLI | |
Accessioni | P18335Primary (citable) accession number: P18335 Secondary accession number(s): Q2M725 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 177 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families