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Protein

Granzyme B

Gene

Gzmb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (By similarity).By similarity

Catalytic activityi

Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1 Publication1
Active sitei109Charge relay system1 Publication1
Active sitei204Charge relay system1 Publication1
Sitei229Mediates preference for Asp-containing substrates1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: RGD

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • granzyme-mediated apoptotic signaling pathway Source: GO_Central
  • natural killer cell mediated cytotoxicity Source: InterPro
  • positive regulation of cell death Source: RGD
  • positive regulation of necroptotic process Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processApoptosis, Cytolysis

Enzyme and pathway databases

BRENDAi3.4.21.79 5301
ReactomeiR-RNO-75108 Activation, myristolyation of BID and translocation to mitochondria

Protein family/group databases

MEROPSiS01.091

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme B (EC:3.4.21.79)
Alternative name(s):
Fragmentin
Natural killer cell protease 1
RNKP-1
Gene namesi
Name:Gzmb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi620018 Gzmb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
PropeptideiPRO_000002742519 – 20Activation peptide1 Publication2
ChainiPRO_000002742621 – 248Granzyme BAdd BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation1 Publication
Disulfide bondi143 ↔ 210PROSITE-ProRule annotation1 Publication
Disulfide bondi174 ↔ 189PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP18291
PRIDEiP18291

Expressioni

Gene expression databases

BgeeiENSRNOG00000045973
ExpressionAtlasiP18291 baseline and differential
GenevisibleiP18291 RN

Interactioni

Protein-protein interaction databases

IntActiP18291, 1 interactor
STRINGi10116.ENSRNOP00000056412

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 41Combined sources7
Beta strandi49 – 56Combined sources8
Beta strandi59 – 62Combined sources4
Helixi64 – 66Combined sources3
Beta strandi69 – 76Combined sources8
Beta strandi88 – 97Combined sources10
Turni103 – 106Combined sources4
Beta strandi111 – 117Combined sources7
Beta strandi142 – 148Combined sources7
Beta strandi150 – 152Combined sources3
Beta strandi162 – 168Combined sources7
Helixi171 – 177Combined sources7
Turni178 – 181Combined sources4
Turni184 – 186Combined sources3
Beta strandi187 – 191Combined sources5
Beta strandi206 – 210Combined sources5
Beta strandi213 – 222Combined sources10
Beta strandi229 – 233Combined sources5
Helixi234 – 237Combined sources4
Helixi238 – 245Combined sources8

3D structure databases

ProteinModelPortaliP18291
SMRiP18291
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18291

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 246Peptidase S1PROSITE-ProRule annotationAdd BLAST226

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOVERGENiHBG013304
InParanoidiP18291
KOiK01353
OrthoDBiEOG091G0G5F
PhylomeDBiP18291
TreeFamiTF333630

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR037553 Granzyme_B
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24271:SF41 PTHR24271:SF41, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLLLLLSF SLAPKTEAGE IIGGHEAKPH SRPYMAYLQI MDEYSGSKKC
60 70 80 90 100
GGFLIREDFV LTAAHCSGSK INVTLGAHNI KEQEKMQQII PVVKIIPHPA
110 120 130 140 150
YNSKTISNDI MLLKLKSKAK RSSAVKPLNL PRRNVKVKPG DVCYVAGWGK
160 170 180 190 200
LGPMGKYSDT LQEVELTVQE DQKCESYLKN YFDKANEICA GDPKIKRASF
210 220 230 240
RGDSGGPLVC KKVAAGIVSY GQNDGSTPRA FTKVSTFLSW IKKTMKKS
Length:248
Mass (Da):27,326
Last modified:November 1, 1990 - v1
Checksum:i6F52089DDACCC88C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98H → F AA sequence (PubMed:1732416).Curated1
Sequence conflicti138K → D AA sequence (PubMed:1732416).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34097 mRNA Translation: AAA42055.1
PIRiA43520
RefSeqiNP_612526.2, NM_138517.3
XP_002728303.2, XM_002728257.5
XP_017460403.1, XM_017604914.1
UniGeneiRn.21395

Genome annotation databases

EnsembliENSRNOT00000041430; ENSRNOP00000040429; ENSRNOG00000049976
ENSRNOT00000059660; ENSRNOP00000056412; ENSRNOG00000045973
GeneIDi100361468
171528
KEGGirno:100361468
rno:171528
UCSCiRGD:620018 rat

Similar proteinsi

Entry informationi

Entry nameiGRAB_RAT
AccessioniPrimary (citable) accession number: P18291
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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