UniProtKB - P18186 (OTC_BACSU)
Protein
Ornithine carbamoyltransferase
Gene
argF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity
Catalytic activityi
- EC:2.1.3.3
: L-arginine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Ornithine carbamoyltransferase (argF), Ornithine carbamoyltransferase (argF)
- Argininosuccinate synthase (argG), Argininosuccinate synthase (argG)
- Argininosuccinate lyase (argH), Argininosuccinate lyase (argH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 84 | Carbamoyl phosphateUniRule annotation | 1 | |
Binding sitei | 108 | Carbamoyl phosphateUniRule annotation | 1 | |
Binding sitei | 166 | OrnithineUniRule annotation | 1 | |
Binding sitei | 230 | OrnithineUniRule annotation | 1 | |
Binding sitei | 298 | Carbamoyl phosphateUniRule annotation | 1 |
GO - Molecular functioni
- amino acid binding Source: InterPro
- ornithine carbamoyltransferase activity Source: GO_Central
GO - Biological processi
- arginine biosynthetic process via ornithine Source: GO_Central
- citrulline biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU11250-MONOMER |
SABIO-RKi | P18186 |
UniPathwayi | UPA00068;UER00112 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:argF Ordered Locus Names:BSU11250 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000112886 | 1 – 319 | Ornithine carbamoyltransferaseAdd BLAST | 319 |
Proteomic databases
PaxDbi | P18186 |
PRIDEi | P18186 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 57 – 60 | Carbamoyl phosphate bindingUniRule annotation | 4 | |
Regioni | 135 – 138 | Carbamoyl phosphate bindingUniRule annotation | 4 | |
Regioni | 234 – 235 | Ornithine bindingUniRule annotation | 2 | |
Regioni | 270 – 271 | Carbamoyl phosphate bindingUniRule annotation | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0078, Bacteria |
InParanoidi | P18186 |
OMAi | DGNNVCN |
PhylomeDBi | P18186 |
Family and domain databases
Gene3Di | 3.40.50.1370, 2 hits |
HAMAPi | MF_01109, OTCase, 1 hit |
InterProi | View protein in InterPro IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR002292, Orn/put_carbamltrans IPR024904, OTCase_ArgI |
Pfami | View protein in Pfam PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00102, OTCASE |
SUPFAMi | SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00658, orni_carb_tr, 1 hit |
PROSITEi | View protein in PROSITE PS00097, CARBAMOYLTRANSFERASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P18186-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHTVTQTSLY GKDLLTLKDL SEEDINALLA EAGELKQNKI QPIFHGKTLA
60 70 80 90 100
MIFEKSSTRT RVSFEAGMAQ LGGSALFLSQ KDLQLGRGET VADTAKVLSG
110 120 130 140 150
YVDAIMIRTF EHEKVEELAK EADIPVINGL TDKYHPCQAL ADLLTIKEIK
160 170 180 190 200
GKLKGVKVAY IGDGNNVAHS LMIGCAKMGC DISIASPKGY EVLDEAAEAA
210 220 230 240 250
KTYALQSGSS VTLTDDPIEA VKDADVIYSD VFTSMGQEAE EQERLAVFAP
260 270 280 290 300
YQVNAALVSH AKPDYTFLHC LPAHREEEVT AEIIDGPNSA VFQQAENRLH
310
VQKALLKAIL YKGESSKNC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53360 Genomic DNA Translation: CAA37444.1 Z26919 Genomic DNA Translation: CAA81542.1 AL009126 Genomic DNA Translation: CAB12966.1 D86376 Genomic DNA Translation: BAA22924.1 |
PIRi | S11000, OWBS |
RefSeqi | NP_389007.1, NC_000964.3 WP_003232980.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB12966; CAB12966; BSU11250 |
GeneIDi | 936386 |
KEGGi | bsu:BSU11250 |
PATRICi | fig|224308.179.peg.1210 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53360 Genomic DNA Translation: CAA37444.1 Z26919 Genomic DNA Translation: CAA81542.1 AL009126 Genomic DNA Translation: CAB12966.1 D86376 Genomic DNA Translation: BAA22924.1 |
PIRi | S11000, OWBS |
RefSeqi | NP_389007.1, NC_000964.3 WP_003232980.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | P18186 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU11250 |
Proteomic databases
PaxDbi | P18186 |
PRIDEi | P18186 |
Genome annotation databases
EnsemblBacteriai | CAB12966; CAB12966; BSU11250 |
GeneIDi | 936386 |
KEGGi | bsu:BSU11250 |
PATRICi | fig|224308.179.peg.1210 |
Phylogenomic databases
eggNOGi | COG0078, Bacteria |
InParanoidi | P18186 |
OMAi | DGNNVCN |
PhylomeDBi | P18186 |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00112 |
BioCyci | BSUB:BSU11250-MONOMER |
SABIO-RKi | P18186 |
Family and domain databases
Gene3Di | 3.40.50.1370, 2 hits |
HAMAPi | MF_01109, OTCase, 1 hit |
InterProi | View protein in InterPro IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR002292, Orn/put_carbamltrans IPR024904, OTCase_ArgI |
Pfami | View protein in Pfam PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00102, OTCASE |
SUPFAMi | SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00658, orni_carb_tr, 1 hit |
PROSITEi | View protein in PROSITE PS00097, CARBAMOYLTRANSFERASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OTC_BACSU | |
Accessioni | P18186Primary (citable) accession number: P18186 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families