UniProtKB - P18157 (GLPK_BACSU)
Protein
Glycerol kinase
Gene
glpK
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation
Catalytic activityi
- EC:2.7.1.30UniRule annotation
Activity regulationi
Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation
: glycerol degradation via glycerol kinase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Glycerol kinase (glpK), Glycerol kinase (glpK)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | SubstrateUniRule annotation | 1 | |
Binding sitei | 16 | ATPUniRule annotation | 1 | |
Binding sitei | 134 | SubstrateUniRule annotation | 1 | |
Binding sitei | 266 | ATPUniRule annotation | 1 | |
Binding sitei | 309 | ATP; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 313 | ATP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 328 | ATPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 14 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 410 – 414 | ATPUniRule annotation | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- glycerol kinase activity Source: UniProtKB
GO - Biological processi
- glycerol-3-phosphate metabolic process Source: UniProtKB-UniRule
- glycerol catabolic process Source: UniProtKB-UniPathway
- glycerol metabolic process Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycerol metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU09290-MONOMER |
UniPathwayi | UPA00618;UER00672 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)Alternative name(s): ATP:glycerol 3-phosphotransferaseUniRule annotation GlycerokinaseUniRule annotation Short name: GKUniRule annotation |
Gene namesi | Name:glpKUniRule annotation Ordered Locus Names:BSU09290 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 230 | H → R: Increased activity. 1 Publication | 1 | |
Mutagenesisi | 232 | F → S: Increased activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059434 | 1 – 496 | Glycerol kinaseAdd BLAST | 496 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 230 | Phosphohistidine; by HPrUniRule annotation | 1 |
Post-translational modificationi
The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P18157 |
PaxDbi | P18157 |
PRIDEi | P18157 |
Interactioni
Subunit structurei
Homotetramer and homodimer (in equilibrium).
UniRule annotationProtein-protein interaction databases
STRINGi | 224308.BSU09290 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 82 – 83 | Substrate bindingUniRule annotation | 2 | |
Regioni | 244 – 245 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the FGGY kinase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0554, Bacteria |
InParanoidi | P18157 |
OMAi | FMLMNIG |
PhylomeDBi | P18157 |
Family and domain databases
HAMAPi | MF_00186, Glycerol_kin, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000577, Carb_kinase_FGGY IPR018485, Carb_kinase_FGGY_C IPR018483, Carb_kinase_FGGY_CS IPR018484, Carb_kinase_FGGY_N IPR005999, Glycerol_kin |
Pfami | View protein in Pfam PF02782, FGGY_C, 1 hit PF00370, FGGY_N, 1 hit |
PIRSFi | PIRSF000538, GlpK, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR01311, glycerol_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00933, FGGY_KINASES_1, 1 hit PS00445, FGGY_KINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P18157-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METYILSLDQ GTTSSRAILF NKEGKIVHSA QKEFTQYFPH PGWVEHNANE
60 70 80 90 100
IWGSVLAVIA SVISESGISA SQIAGIGITN QRETTVVWDK DTGSPVYNAI
110 120 130 140 150
VWQSRQTSGI CEELREKGYN DKFREKTGLL IDPYFSGTKV KWILDNVEGA
160 170 180 190 200
REKAEKGELL FGTIDTWLIW KMSGGKAHVT DYSNASRTLM FNIYDLKWDD
210 220 230 240 250
ELLDILGVPK SMLPEVKPSS HVYAETVDYH FFGKNIPIAG AAGDQQSALF
260 270 280 290 300
GQACFEEGMG KNTYGTGCFM LMNTGEKAIK SEHGLLTTIA WGIDGKVNYA
310 320 330 340 350
LEGSIFVAGS AIQWLRDGLR MFQDSSLSES YAEKVDSTDG VYVVPAFVGL
360 370 380 390 400
GTPYWDSDVR GSVFGLTRGT TKEHFIRATL ESLAYQTKDV LDAMEADSNI
410 420 430 440 450
SLKTLRVDGG AVKNNFLMQF QGDLLNVPVE RPEINETTAL GAAYLAGIAV
460 470 480 490
GFWKDRSEIA NQWNLDKRFE PELEEEKRNE LYKGWQKAVK AAMAFK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 201 | E → Q in AAA22486 (PubMed:2127799).Curated | 1 | |
Sequence conflicti | 201 | E → Q in CAA74429 (PubMed:9579061).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34393 Genomic DNA Translation: AAA22486.1 Y14079 Genomic DNA Translation: CAA74429.1 AL009126 Genomic DNA Translation: CAB12757.2 M99611 Genomic DNA Translation: AAA22491.1 |
PIRi | B45868 |
RefSeqi | NP_388810.2, NC_000964.3 WP_003233384.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB12757; CAB12757; BSU_09290 |
GeneIDi | 939741 |
KEGGi | bsu:BSU09290 |
PATRICi | fig|224308.179.peg.1002 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34393 Genomic DNA Translation: AAA22486.1 Y14079 Genomic DNA Translation: CAA74429.1 AL009126 Genomic DNA Translation: CAB12757.2 M99611 Genomic DNA Translation: AAA22491.1 |
PIRi | B45868 |
RefSeqi | NP_388810.2, NC_000964.3 WP_003233384.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | P18157 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU09290 |
Proteomic databases
jPOSTi | P18157 |
PaxDbi | P18157 |
PRIDEi | P18157 |
Genome annotation databases
EnsemblBacteriai | CAB12757; CAB12757; BSU_09290 |
GeneIDi | 939741 |
KEGGi | bsu:BSU09290 |
PATRICi | fig|224308.179.peg.1002 |
Phylogenomic databases
eggNOGi | COG0554, Bacteria |
InParanoidi | P18157 |
OMAi | FMLMNIG |
PhylomeDBi | P18157 |
Enzyme and pathway databases
UniPathwayi | UPA00618;UER00672 |
BioCyci | BSUB:BSU09290-MONOMER |
Family and domain databases
HAMAPi | MF_00186, Glycerol_kin, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000577, Carb_kinase_FGGY IPR018485, Carb_kinase_FGGY_C IPR018483, Carb_kinase_FGGY_CS IPR018484, Carb_kinase_FGGY_N IPR005999, Glycerol_kin |
Pfami | View protein in Pfam PF02782, FGGY_C, 1 hit PF00370, FGGY_N, 1 hit |
PIRSFi | PIRSF000538, GlpK, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR01311, glycerol_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00933, FGGY_KINASES_1, 1 hit PS00445, FGGY_KINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLPK_BACSU | |
Accessioni | P18157Primary (citable) accession number: P18157 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families