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Protein

Nicotinate phosphoribosyltransferase

Gene

pncB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.UniRule annotation1 Publication

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.UniRule annotation1 Publication

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate phosphoribosyltransferase (pncB)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • NAD biosynthetic process Source: UniProtKB
  • nicotinate nucleotide biosynthetic process Source: InterPro
  • response to heat Source: EcoCyc

Keywordsi

Molecular functionLigase, Transferase
Biological processPyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:NICOTINATEPRIBOSYLTRANS-MONOMER
MetaCyc:NICOTINATEPRIBOSYLTRANS-MONOMER
BRENDAi6.3.4.21 2026
UniPathwayiUPA00253; UER00457

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferaseUniRule annotation (EC:6.3.4.21UniRule annotation)
Short name:
NAPRTaseUniRule annotation
Gene namesi
Name:pncBUniRule annotation
Ordered Locus Names:b0931, JW0914
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10742 pncB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002058282 – 400Nicotinate phosphoribosyltransferaseAdd BLAST399

Proteomic databases

PaxDbiP18133
PRIDEiP18133

Interactioni

Protein-protein interaction databases

BioGridi4261881, 11 interactors
DIPiDIP-10521N
IntActiP18133, 19 interactors
STRINGi316385.ECDH10B_1001

Structurei

3D structure databases

ProteinModelPortaliP18133
SMRiP18133
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RH7 Bacteria
COG1488 LUCA
HOGENOMiHOG000284928
InParanoidiP18133
KOiK00763
OMAiKLTMQCA
PhylomeDBiP18133

Family and domain databases

CDDicd01401 PncB_like, 1 hit
HAMAPiMF_00570 NAPRTase, 1 hit
InterProiView protein in InterPro
IPR006406 Nic_PRibTrfase
IPR007229 Nic_PRibTrfase-Fam
IPR036068 Nicotinate_pribotase_like_C
PANTHERiPTHR11098 PTHR11098, 1 hit
PfamiView protein in Pfam
PF04095 NAPRTase, 1 hit
PIRSFiPIRSF000484 NAPRT, 1 hit
SUPFAMiSSF51690 SSF51690, 1 hit
TIGRFAMsiTIGR01514 NAPRTase, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY
60 70 80 90 100
ADAIREQVQA MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV
110 120 130 140 150
SNDNGKLDIR LSGPWREVIL WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL
160 170 180 190 200
ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF SREVQETIVK RLQQESWFVG
210 220 230 240 250
TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA LAAWLEEYPD
260 270 280 290 300
QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK
310 320 330 340 350
LGIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK
360 370 380 390 400
PLNIVIKLVE CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS
Length:400
Mass (Da):45,897
Last modified:January 23, 2007 - v3
Checksum:i1D5FFC61EBAAE1D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05568 Genomic DNA Translation: AAA24400.1
U00096 Genomic DNA Translation: AAC74017.1
AP009048 Genomic DNA Translation: BAA35683.1
PIRiJQ0756
RefSeqiNP_415451.1, NC_000913.3
WP_001307697.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74017; AAC74017; b0931
BAA35683; BAA35683; BAA35683
GeneIDi946648
KEGGiecj:JW0914
eco:b0931
PATRICifig|1411691.4.peg.1344

Similar proteinsi

Entry informationi

Entry nameiPNCB_ECOLI
AccessioniPrimary (citable) accession number: P18133
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 156 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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