UniProtKB - P18090 (ADRB1_RAT)
Protein
Beta-1 adrenergic receptor
Gene
Adrb1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling (By similarity). Involved in the regulation of sleep/wake behaviors (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 138 | Agonist or antagonistBy similarity | 1 | |
Binding sitei | 143 | Agonist or antagonistBy similarity | 1 |
GO - Molecular functioni
- alpha-2A adrenergic receptor binding Source: RGD
- beta1-adrenergic receptor activity Source: RGD
- G protein-coupled neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential Source: RGD
- G protein-coupled receptor activity Source: GO_Central
- PDZ domain binding Source: RGD
- protein heterodimerization activity Source: RGD
- Ras guanyl-nucleotide exchange factor activity Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: RGD
- aging Source: RGD
- apoptotic process Source: RGD
- brown fat cell differentiation Source: RGD
- diet induced thermogenesis Source: RGD
- fear response Source: RGD
- glycogen catabolic process Source: RGD
- G protein-coupled receptor signaling pathway Source: RGD
- heat generation Source: RGD
- inhibitory postsynaptic potential Source: RGD
- lipid homeostasis Source: RGD
- memory Source: RGD
- negative regulation of multicellular organism growth Source: RGD
- negative regulation of smooth muscle contraction Source: RGD
- negative regulation of urine volume Source: RGD
- norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure Source: RGD
- positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: RGD
- positive regulation of adenylate cyclase activity Source: RGD
- positive regulation of apoptotic process Source: RGD
- positive regulation of calcium ion import across plasma membrane Source: RGD
- positive regulation of cation channel activity Source: RGD
- positive regulation of cell growth involved in cardiac muscle cell development Source: RGD
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of heart rate Source: RGD
- positive regulation of heart rate by epinephrine-norepinephrine Source: RGD
- positive regulation of long-term synaptic potentiation Source: RGD
- positive regulation of renin secretion into blood stream Source: RGD
- positive regulation of saliva secretion Source: RGD
- positive regulation of systemic arterial blood pressure Source: RGD
- positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: RGD
- positive regulation of the force of heart contraction by norepinephrine Source: RGD
- protein localization to organelle Source: RGD
- regulation of calcium ion transport Source: RGD
- regulation of cardiac muscle cell contraction Source: RGD
- regulation of circadian sleep/wake cycle, sleep Source: UniProtKB
- response to cold Source: RGD
- Rho protein signal transduction Source: RGD
- sensory perception of pain Source: RGD
- wound healing Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390696, Adrenoceptors |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-1 adrenergic receptorAlternative name(s): Beta-1 adrenoreceptor Short name: Beta-1 adrenoceptor |
Gene namesi | Name:Adrb1 Synonyms:Adrb1r |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2059, Adrb1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endosome
- Early endosome By similarity
Note: Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression (By similarity). Colocalizes with RAPGEF2 at the plasma membrane.By similarity
Endosome
- early endosome Source: UniProtKB
Nucleus
- nucleus Source: RGD
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: RGD
- intracellular membrane-bounded organelle Source: RGD
- membrane Source: RGD
- postsynapse Source: GOC
- Schaffer collateral - CA1 synapse Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 55 | ExtracellularBy similarityAdd BLAST | 55 | |
Transmembranei | 56 – 84 | Helical; Name=1By similarityAdd BLAST | 29 | |
Topological domaini | 85 – 93 | CytoplasmicBy similarity | 9 | |
Transmembranei | 94 – 120 | Helical; Name=2By similarityAdd BLAST | 27 | |
Topological domaini | 121 – 132 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 133 – 154 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 155 – 172 | CytoplasmicBy similarityAdd BLAST | 18 | |
Transmembranei | 173 – 196 | Helical; Name=4By similarityAdd BLAST | 24 | |
Topological domaini | 197 – 222 | ExtracellularBy similarityAdd BLAST | 26 | |
Transmembranei | 223 – 248 | Helical; Name=5By similarityAdd BLAST | 26 | |
Topological domaini | 249 – 308 | CytoplasmicBy similarityAdd BLAST | 60 | |
Transmembranei | 309 – 338 | Helical; Name=6By similarityAdd BLAST | 30 | |
Topological domaini | 339 – 343 | ExtracellularBy similarity | 5 | |
Transmembranei | 344 – 366 | Helical; Name=7By similarityAdd BLAST | 23 | |
Topological domaini | 367 – 466 | CytoplasmicBy similarityAdd BLAST | 100 |
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 466 | V → A: Abolishes interaction with MAGI3. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3252 |
DrugCentrali | P18090 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069124 | 1 – 466 | Beta-1 adrenergic receptorAdd BLAST | 466 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Disulfide bondi | 131 ↔ 216 | PROSITE-ProRule annotation | ||
Disulfide bondi | 209 ↔ 215 | PROSITE-ProRule annotation | ||
Modified residuei | 296 | Phosphoserine; by PKASequence analysis | 1 | |
Modified residuei | 301 | Phosphoserine; by PKASequence analysis | 1 | |
Lipidationi | 381 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 401 | Phosphoserine; by PKASequence analysis | 1 | |
Modified residuei | 417 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P18090 |
PRIDEi | P18090 |
PTM databases
GlyGeni | P18090, 1 site |
iPTMneti | P18090 |
PhosphoSitePlusi | P18090 |
Expressioni
Tissue specificityi
Expressed in cortical neurons and coronary artery smooth muscle cells (at protein level).1 Publication
Interactioni
Subunit structurei
Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct (By similarity).
Interacts with GOPC, MAGI3 and DLG4.
By similarity1 PublicationBinary interactionsi
Hide detailsGO - Molecular functioni
- alpha-2A adrenergic receptor binding Source: RGD
- PDZ domain binding Source: RGD
- protein heterodimerization activity Source: RGD
- Ras guanyl-nucleotide exchange factor activity Source: UniProtKB
Protein-protein interaction databases
DIPi | DIP-36293N |
IntActi | P18090, 2 interactors |
STRINGi | 10116.ENSRNOP00000022813 |
Chemistry databases
BindingDBi | P18090 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 218 – 232 | Agonist and antagonist bindingBy similarityAdd BLAST | 15 | |
Regioni | 326 – 333 | Agonist and antagonist bindingBy similarity | 8 | |
Regioni | 352 – 356 | Agonist and antagonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 463 – 466 | PDZ-BindingBy similarity | 4 |
Domaini
The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P18090 |
OrthoDBi | 750855at2759 |
PhylomeDBi | P18090 |
TreeFami | TF316350 |
Family and domain databases
InterProi | View protein in InterPro IPR002233, ADR_fam IPR000507, ADRB1_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01103, ADRENERGICR PR00561, ADRENRGCB1AR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P18090-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGAGALALGA SEPCNLSSAA PLPDGAATAA RLLVLASPPA SLLPPASEGS
60 70 80 90 100
APLSQQWTAG MGLLLALIVL LIVVGNVLVI VAIAKTPRLQ TLTNLFIMSL
110 120 130 140 150
ASADLVMGLL VVPFGATIVV WGRWEYGSFF CELWTSVDVL CVTASIETLC
160 170 180 190 200
VIALDRYLAI TLPFRYQSLL TRARARALVC TVWAISALVS FLPILMHWWR
210 220 230 240 250
AESDEARRCY NDPKCCDFVT NRAYAIASSV VSFYVPLCIM AFVYLRVFRE
260 270 280 290 300
AQKQVKKIDS CERRFLTGPP RPPSPAPSPS PGPPRPADSL ANGRSSKRRP
310 320 330 340 350
SRLVALREQK ALKTLGIIMG VFTLCWLPFF LANVVKAFHR DLVPDRLFVF
360 370 380 390 400
FNWLGYANSA FNPIIYCRSP DFRKAFQRLL CCARRAACRR RAAHGDRPRA
410 420 430 440 450
SGCLARAGPP PSPGAPSDDD DDDAGATPPA RLLEPWAGCN GGTTTVDSDS
460
SLDEPGRQGF SSESKV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JSP7 | A0A0G2JSP7_RAT | Beta-1 adrenergic receptor | Adrb1 | 466 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 162 | L → S in BAA00527 (PubMed:2167473).Curated | 1 | |
Sequence conflicti | 267 | T → S in BAA00527 (PubMed:2167473).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05561 Genomic DNA Translation: AAA40792.1 D00634 Genomic DNA Translation: BAA00527.1 |
PIRi | S12591 |
RefSeqi | NP_036833.1, NM_012701.1 |
Genome annotation databases
GeneIDi | 24925 |
KEGGi | rno:24925 |
UCSCi | RGD:2059, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05561 Genomic DNA Translation: AAA40792.1 D00634 Genomic DNA Translation: BAA00527.1 |
PIRi | S12591 |
RefSeqi | NP_036833.1, NM_012701.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FF9 | model | - | A | 34-384 | [»] | |
SMRi | P18090 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-36293N |
IntActi | P18090, 2 interactors |
STRINGi | 10116.ENSRNOP00000022813 |
Chemistry databases
BindingDBi | P18090 |
ChEMBLi | CHEMBL3252 |
DrugCentrali | P18090 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P18090, 1 site |
iPTMneti | P18090 |
PhosphoSitePlusi | P18090 |
Proteomic databases
PaxDbi | P18090 |
PRIDEi | P18090 |
Genome annotation databases
GeneIDi | 24925 |
KEGGi | rno:24925 |
UCSCi | RGD:2059, rat |
Organism-specific databases
CTDi | 153 |
RGDi | 2059, Adrb1 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P18090 |
OrthoDBi | 750855at2759 |
PhylomeDBi | P18090 |
TreeFami | TF316350 |
Enzyme and pathway databases
Reactomei | R-RNO-390696, Adrenoceptors |
Miscellaneous databases
PROi | PR:P18090 |
Family and domain databases
InterProi | View protein in InterPro IPR002233, ADR_fam IPR000507, ADRB1_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01103, ADRENERGICR PR00561, ADRENRGCB1AR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADRB1_RAT | |
Accessioni | P18090Primary (citable) accession number: P18090 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families