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Protein

ADP-ribosylation factor 4

Gene

ARF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Caution

Was originally thought to be ARF2.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTP8
Nucleotide bindingi67 – 71GTP5
Nucleotide bindingi126 – 129GTP4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5620916 VxPx cargo-targeting to cilium
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 4
Gene namesi
Name:ARF4
Synonyms:ARF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000168374.10
HGNCiHGNC:655 ARF4
MIMi601177 gene
neXtProtiNX_P18085

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi378
OpenTargetsiENSG00000168374
PharmGKBiPA24937

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiARF4
DMDMi114123

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002073912 – 180ADP-ribosylation factor 4Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei147PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP18085
MaxQBiP18085
PaxDbiP18085
PeptideAtlasiP18085
PRIDEiP18085
ProteomicsDBi53547
TopDownProteomicsiP18085

PTM databases

iPTMnetiP18085
PhosphoSitePlusiP18085
SwissPalmiP18085

Expressioni

Gene expression databases

BgeeiENSG00000168374 Expressed in 238 organ(s), highest expression level in tibia
CleanExiHS_ARF4
ExpressionAtlasiP18085 baseline and differential
GenevisibleiP18085 HS

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106873, 53 interactors
DIPiDIP-38214N
IntActiP18085, 31 interactors
MINTiP18085
STRINGi9606.ENSP00000306010

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP18085
SMRiP18085
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18085

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP18085
KOiK07939
OMAiWHIESTC
OrthoDBiEOG091G0S7S
PhylomeDBiP18085
TreeFamiTF300808

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

P18085-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
QEVADELQKM LLVDELRDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN
160 170 180
RTWYVQATCA TQGTGLYEGL DWLSNELSKR
Length:180
Mass (Da):20,511
Last modified:January 23, 2007 - v3
Checksum:i3A43EB1F37A81BD9
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JPM4C9JPM4_HUMAN
ADP-ribosylation factor 4
ARF4
127Annotation score:
C9JAK5C9JAK5_HUMAN
ADP-ribosylation factor 4
ARF4
153Annotation score:
F8WDB3F8WDB3_HUMAN
ADP-ribosylation factor 4
ARF4
59Annotation score:
U3KQF2U3KQF2_HUMAN
ADP-ribosylation factor 4
ARF4
71Annotation score:
C9J6P1C9J6P1_HUMAN
ADP-ribosylation factor 4
ARF4
22Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04831768V → A. Corresponds to variant dbSNP:rs11550597Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36341 mRNA Translation: AAA53081.1
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA Translation: AAD54674.1
AF493883 mRNA Translation: AAM12597.1
AK313008 mRNA Translation: BAG35844.1
BC003364 mRNA Translation: AAH03364.1
BC008753 mRNA Translation: AAH08753.1
BC016325 mRNA Translation: AAH16325.1
BC022866 mRNA Translation: AAH22866.1
CCDSiCCDS2884.1
PIRiB38622
RefSeqiNP_001651.1, NM_001660.3
UniGeneiHs.652183

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374
GeneIDi378
KEGGihsa:378
UCSCiuc003dix.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36341 mRNA Translation: AAA53081.1
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA Translation: AAD54674.1
AF493883 mRNA Translation: AAM12597.1
AK313008 mRNA Translation: BAG35844.1
BC003364 mRNA Translation: AAH03364.1
BC008753 mRNA Translation: AAH08753.1
BC016325 mRNA Translation: AAH16325.1
BC022866 mRNA Translation: AAH22866.1
CCDSiCCDS2884.1
PIRiB38622
RefSeqiNP_001651.1, NM_001660.3
UniGeneiHs.652183

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6XX-ray2.70A/B1-180[»]
ProteinModelPortaliP18085
SMRiP18085
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106873, 53 interactors
DIPiDIP-38214N
IntActiP18085, 31 interactors
MINTiP18085
STRINGi9606.ENSP00000306010

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

PTM databases

iPTMnetiP18085
PhosphoSitePlusiP18085
SwissPalmiP18085

Polymorphism and mutation databases

BioMutaiARF4
DMDMi114123

Proteomic databases

EPDiP18085
MaxQBiP18085
PaxDbiP18085
PeptideAtlasiP18085
PRIDEiP18085
ProteomicsDBi53547
TopDownProteomicsiP18085

Protocols and materials databases

DNASUi378
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374
GeneIDi378
KEGGihsa:378
UCSCiuc003dix.5 human

Organism-specific databases

CTDi378
DisGeNETi378
EuPathDBiHostDB:ENSG00000168374.10
GeneCardsiARF4
HGNCiHGNC:655 ARF4
MIMi601177 gene
neXtProtiNX_P18085
OpenTargetsiENSG00000168374
PharmGKBiPA24937
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP18085
KOiK07939
OMAiWHIESTC
OrthoDBiEOG091G0S7S
PhylomeDBiP18085
TreeFamiTF300808

Enzyme and pathway databases

ReactomeiR-HSA-5620916 VxPx cargo-targeting to cilium
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Miscellaneous databases

ChiTaRSiARF4 human
EvolutionaryTraceiP18085
GeneWikiiARF4
GenomeRNAii378
PROiPR:P18085
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168374 Expressed in 238 organ(s), highest expression level in tibia
CleanExiHS_ARF4
ExpressionAtlasiP18085 baseline and differential
GenevisibleiP18085 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARF4_HUMAN
AccessioniPrimary (citable) accession number: P18085
Secondary accession number(s): B2R7J7, P21371
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 194 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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