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Protein

60S ribosomal protein L35a

Gene

RPL35A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.1 Publication

Miscellaneous

Knockdown of RPL35A in hematopoietic cell lines results in decreased cell proliferation, increased apoptosis, decreased biogenesis of mature 60S ribosomal subunit, and abnormal processing of large ribosomal subunit rRNA.

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: GO_Central
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
  • ribosomal large subunit biogenesis Source: UniProtKB
  • rRNA processing Source: UniProtKB
  • SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  • translation Source: UniProtKB
  • translational initiation Source: Reactome

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, tRNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902 Peptide chain elongation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823 Viral mRNA Translation
R-HSA-2408557 Selenocysteine synthesis
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35a
Alternative name(s):
Cell growth-inhibiting gene 33 protein
Large ribosomal subunit protein eL331 Publication
Gene namesi
Name:RPL35A
ORF Names:GIG33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000182899.14
HGNCiHGNC:10345 RPL35A
MIMi180468 gene
neXtProtiNX_P18077

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Involvement in diseasei

Diamond-Blackfan anemia 5 (DBA5)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
See also OMIM:612528
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05544627Missing in DBA5. 1 Publication1
Natural variantiVAR_05544733V → I in DBA5; may result in aberrant splicing. 1 PublicationCorresponds to variant dbSNP:rs116840808EnsemblClinVar.1

Keywords - Diseasei

Diamond-Blackfan anemia, Disease mutation

Organism-specific databases

DisGeNETi6165
GeneReviewsiRPL35A
MalaCardsiRPL35A
MIMi612528 phenotype
OpenTargetsiENSG00000182899
Orphaneti124 Blackfan-Diamond anemia
PharmGKBiPA34728

Polymorphism and mutation databases

BioMutaiRPL35A

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927961 – 11060S ribosomal protein L35aAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei63N6-acetyllysine; alternateBy similarity1
Modified residuei63N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP18077
MaxQBiP18077
PaxDbiP18077
PeptideAtlasiP18077
PRIDEiP18077
ProteomicsDBi53545
TopDownProteomicsiP18077

2D gel databases

SWISS-2DPAGEiP18077

PTM databases

iPTMnetiP18077
PhosphoSitePlusiP18077
SwissPalmiP18077

Expressioni

Gene expression databases

BgeeiENSG00000182899
CleanExiHS_RPL35A
ExpressionAtlasiP18077 baseline and differential
GenevisibleiP18077 HS

Interactioni

Protein-protein interaction databases

BioGridi112084, 95 interactors
CORUMiP18077
IntActiP18077, 19 interactors
MINTiP18077
STRINGi9606.ENSP00000393393

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UG0electron microscopy-Lf1-110[»]
4V6Xelectron microscopy5.00Cf1-110[»]
5AJ0electron microscopy3.50Af1-110[»]
5LKSelectron microscopy3.60Lf1-110[»]
5T2Celectron microscopy3.60Z1-110[»]
6EK0electron microscopy2.90Lf1-110[»]
ProteinModelPortaliP18077
SMRiP18077
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0887 Eukaryota
COG2451 LUCA
GeneTreeiENSGT00390000016972
HOGENOMiHOG000195636
HOVERGENiHBG054581
InParanoidiP18077
KOiK02917
OMAiVRAIWGK
OrthoDBiEOG091G112U
PhylomeDBiP18077
TreeFamiTF300104

Family and domain databases

Gene3Di2.40.10.190, 1 hit
HAMAPiMF_00573 Ribosomal_L35Ae, 1 hit
InterProiView protein in InterPro
IPR038661 L35A_sf
IPR001780 Ribosomal_L35A
IPR018266 Ribosomal_L35Ae_CS
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR10902 PTHR10902, 1 hit
PfamiView protein in Pfam
PF01247 Ribosomal_L35Ae, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012670 Ribosomal_L35Ae, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
PROSITEiView protein in PROSITE
PS01105 RIBOSOMAL_L35AE, 1 hit

Sequencei

Sequence statusi: Complete.

P18077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV
60 70 80 90 100
YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFRS NLPAKAIGHR
110
IRVMLYPSRI
Length:110
Mass (Da):12,538
Last modified:July 26, 2002 - v2
Checksum:iF32E4A26A25E79E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85R → L in CAA37138 (PubMed:2388839).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05544627Missing in DBA5. 1 Publication1
Natural variantiVAR_05544733V → I in DBA5; may result in aberrant splicing. 1 PublicationCorresponds to variant dbSNP:rs116840808EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA Translation: CAA37138.1
AY871273 mRNA Translation: AAX11429.1
CH471252 Genomic DNA Translation: EAW92249.1
BC001037 mRNA Translation: AAH01037.1
BC010949 mRNA Translation: AAH10949.1
BC017093 mRNA Translation: AAH17093.1
BC061890 mRNA Translation: AAH61890.1
X94619 Genomic DNA Translation: CAA64325.1
CCDSiCCDS33930.1
PIRiS12710 R5HU35
RefSeqiNP_000987.2, NM_000996.2
NP_001303240.1, NM_001316311.1
UniGeneiHs.529631

Genome annotation databases

EnsembliENST00000448864; ENSP00000393393; ENSG00000182899
ENST00000464167; ENSP00000419117; ENSG00000182899
ENST00000647248; ENSP00000495672; ENSG00000182899
GeneIDi6165
KEGGihsa:6165
UCSCiuc003fyr.4 human

Similar proteinsi

Entry informationi

Entry nameiRL35A_HUMAN
AccessioniPrimary (citable) accession number: P18077
Secondary accession number(s): Q08ES9, Q9BVN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 26, 2002
Last modified: June 20, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

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