UniProtKB - P18052 (PTPRA_MOUSE)
Protein
Receptor-type tyrosine-protein phosphatase alpha
Gene
Ptpra
Organism
Mus musculus (Mouse)
Status
Functioni
Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (PubMed:22801373). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (PubMed:22801373).1 Publication
Catalytic activityi
- EC:3.1.3.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 437 | SubstrateBy similarity | 1 | |
Active sitei | 469 | Phosphocysteine intermediateBy similarity | 1 | |
Binding sitei | 513 | SubstrateBy similarity | 1 | |
Active sitei | 759 | Phosphocysteine intermediateBy similarity | 1 |
GO - Molecular functioni
- protein-containing complex binding Source: MGI
- protein tyrosine phosphatase activity Source: UniProtKB
GO - Biological processi
- insulin receptor signaling pathway Source: MGI
- integrin-mediated signaling pathway Source: UniProtKB
- modulation of chemical synaptic transmission Source: SynGO
- positive regulation of oligodendrocyte differentiation Source: MGI
- protein dephosphorylation Source: GO_Central
- protein phosphorylation Source: MGI
- regulation of focal adhesion assembly Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
Reactomei | R-MMU-375165, NCAM signaling for neurite out-growth R-MMU-5673001, RAF/MAP kinase cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Receptor-type tyrosine-protein phosphatase alpha (EC:3.1.3.48)Short name: Protein-tyrosine phosphatase alpha Short name: R-PTP-alpha Alternative name(s): LCA-related phosphatase PTPTY-28 |
Gene namesi | Name:Ptpra Synonyms:Lrp, Ptpa |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97808, Ptpra |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Other locations
- focal adhesion 1 Publication
Note: Localizes to focal adhesion sites following integrin engagement.1 Publication
Plasma Membrane
- integral component of synaptic membrane Source: SynGO
- plasma membrane Source: MGI
Other locations
- focal adhesion Source: UniProtKB
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: UniProtKB
- receptor complex Source: MGI
- Schaffer collateral - CA1 synapse Source: SynGO
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 142 | ExtracellularSequence analysisAdd BLAST | 123 | |
Transmembranei | 143 – 166 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 167 – 829 | CytoplasmicSequence analysisAdd BLAST | 663 |
Keywords - Cellular componenti
Cell junction, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | CuratedAdd BLAST | 19 | |
ChainiPRO_0000025434 | 20 – 829 | Receptor-type tyrosine-protein phosphatase alphaAdd BLAST | 810 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 21 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 47 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 68 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 80 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 86 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 104 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 124 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 202 | PhosphoserineCombined sources | 1 | |
Modified residuei | 204 | PhosphoserineCombined sources | 1 | |
Modified residuei | 825 | PhosphotyrosineCombined sources1 Publication | 1 |
Post-translational modificationi
Integrin binding to extracellular matrix induces phosphorylation at Tyr-825 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.1 Publication
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P18052 |
MaxQBi | P18052 |
PaxDbi | P18052 |
PeptideAtlasi | P18052 |
PRIDEi | P18052 |
PTM databases
GlyConnecti | 2665, 1 N-Linked glycan (1 site) |
GlyGeni | P18052, 8 sites |
iPTMneti | P18052 |
PhosphoSitePlusi | P18052 |
Expressioni
Tissue specificityi
Widely expressed. Highest expression in brain and kidney.3 Publications
Gene expression databases
Bgeei | ENSMUSG00000027303, Expressed in lens of camera-type eye and 304 other tissues |
ExpressionAtlasi | P18052, baseline and differential |
Genevisiblei | P18052, MM |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP18052
Protein-protein interaction databases
BioGRIDi | 202491, 30 interactors |
CORUMi | P18052 |
IntActi | P18052, 12 interactors |
MINTi | P18052 |
STRINGi | 10090.ENSMUSP00000076533 |
Miscellaneous databases
RNActi | P18052, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P18052 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P18052 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 232 – 528 | Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST | 297 | |
Domaini | 560 – 818 | Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST | 259 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 469 – 475 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4228, Eukaryota |
GeneTreei | ENSGT00940000159585 |
InParanoidi | P18052 |
OMAi | QGRMGVC |
TreeFami | TF351829 |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR016336, Tyr_Pase_rcpt_a/e-type IPR027262, Tyr_Pase_rcpt_alpha IPR000387, TYR_PHOSPHATASE_dom |
PANTHERi | PTHR19134:SF433, PTHR19134:SF433, 1 hit |
Pfami | View protein in Pfam PF00102, Y_phosphatase, 2 hits |
PIRSFi | PIRSF500808, PTPR_alpha, 1 hit PIRSF002006, PTPR_alpha_epsilon, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 2 hits SM00404, PTPc_motif, 2 hits |
SUPFAMi | SSF52799, SSF52799, 2 hits |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 2 hits PS50056, TYR_PHOSPHATASE_2, 2 hits PS50055, TYR_PHOSPHATASE_PTP, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P18052-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSWFILVLF GSGLIHVSAN NATTVSPSLG TTRLIKTSTT ELAKEENKTS
60 70 80 90 100
NSTSSVISLS VAPTFSPNLT LEPTYVTTVN SSHSDNGTRR AASTESGGTT
110 120 130 140 150
ISPNGSWLIE NQFTDAITEP WEGNSSTAAT TPETFPPADE TPIIAVMVAL
160 170 180 190 200
SSLLVIVFII IVLYMLRFKK YKQAGSHSNS FRLSNGRTED VEPQSVPLLA
210 220 230 240 250
RSPSTNRKYP PLPVDKLEEE INRRMADDNK LFREEFNALP ACPIQATCEA
260 270 280 290 300
ASKEENKEKN RYVNILPFLS LAVSKDAVKA LNKTTPLLER RFIGKSNSRG
310 320 330 340 350
CLSDDHSRVH LTPVEGVPDS DYINASFING YQEKNKFIAA QGPKEETVND
360 370 380 390 400
FWRMIWEQNT ATIVMVTNLK ERKECKCAQY WPDQGCWTYG NVRVSVEDVT
410 420 430 440 450
VLVDYTVRKF CIQQVGDVTN RKPQRLITQF HFTSWPDFGV PFTPIGMLKF
460 470 480 490 500
LKKVKACNPQ YAGAIVVHCS AGVGRTGTFV VIDAMLDMMH SERKVDVYGF
510 520 530 540 550
VSRIRAQRCQ MVQTDMQYVF IYQALLEHYL YGDTELEVTS LETHLQKIYN
560 570 580 590 600
KIPGTSNNGL EEEFKKLTSI KIQNDKMRTG NLPANMKKNR VLQIIPYEFN
610 620 630 640 650
RVIIPVKRGE ENTDYVNASF IDGYRQKDSY IASQGPLLHT IEDFWRMIWE
660 670 680 690 700
WKSCSIVMLT ELEERGQEKC AQYWPSDGLV SYGDITVELK KEEECESYTV
710 720 730 740 750
RDLLVTNTRE NKSRQIRQFH FHGWPEVGIP SDGKGMINII AAVQKQQQQS
760 770 780 790 800
GNHPITVHCS AGAGRTGTFC ALSTVLERVK AEGILDVFQT VKSLRLQRPH
810 820
MVQTLEQYEF CYKVVQEYID AFSDYANFK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ91V35 | Q91V35_MOUSE | Receptor-type tyrosine-protein phos... | Ptpra | 793 | Annotation score: | ||
A0A2R8VHH6 | A0A2R8VHH6_MOUSE | Protein-tyrosine-phosphatase | Ptpra | 374 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 231 | L → I in AAA39448 (PubMed:2162042).Curated | 1 | |
Sequence conflicti | 326 | S → G (PubMed:1590786).Curated | 1 | |
Sequence conflicti | 411 | C → S (PubMed:2166945).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011880 | 268 | F → Y in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_005146 | 269 – 303 | Missing in isoform 2. 2 PublicationsAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36033 mRNA Translation: AAA39448.1 M36034 Genomic DNA Translation: AAA39449.2 L13607 Genomic DNA No translation available. AL731707 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL28272.1 Z23054 mRNA Translation: CAA80589.1 Z23055 mRNA Translation: CAA80590.1 |
CCDSi | CCDS16743.1 [P18052-1] |
PIRi | A47373 |
RefSeqi | NP_033006.2, NM_008980.2 [P18052-1] |
Genome annotation databases
Ensembli | ENSMUST00000077303; ENSMUSP00000076533; ENSMUSG00000027303 [P18052-1] |
GeneIDi | 19262 |
KEGGi | mmu:19262 |
UCSCi | uc008mjc.2, mouse [P18052-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36033 mRNA Translation: AAA39448.1 M36034 Genomic DNA Translation: AAA39449.2 L13607 Genomic DNA No translation available. AL731707 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL28272.1 Z23054 mRNA Translation: CAA80589.1 Z23055 mRNA Translation: CAA80590.1 |
CCDSi | CCDS16743.1 [P18052-1] |
PIRi | A47373 |
RefSeqi | NP_033006.2, NM_008980.2 [P18052-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1P15 | X-ray | 2.00 | A/B | 577-829 | [»] | |
1YFO | X-ray | 2.25 | A/B | 202-539 | [»] | |
SMRi | P18052 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202491, 30 interactors |
CORUMi | P18052 |
IntActi | P18052, 12 interactors |
MINTi | P18052 |
STRINGi | 10090.ENSMUSP00000076533 |
PTM databases
GlyConnecti | 2665, 1 N-Linked glycan (1 site) |
GlyGeni | P18052, 8 sites |
iPTMneti | P18052 |
PhosphoSitePlusi | P18052 |
Proteomic databases
jPOSTi | P18052 |
MaxQBi | P18052 |
PaxDbi | P18052 |
PeptideAtlasi | P18052 |
PRIDEi | P18052 |
Protocols and materials databases
Antibodypediai | 7130, 496 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000077303; ENSMUSP00000076533; ENSMUSG00000027303 [P18052-1] |
GeneIDi | 19262 |
KEGGi | mmu:19262 |
UCSCi | uc008mjc.2, mouse [P18052-1] |
Organism-specific databases
CTDi | 5786 |
MGIi | MGI:97808, Ptpra |
Phylogenomic databases
eggNOGi | KOG4228, Eukaryota |
GeneTreei | ENSGT00940000159585 |
InParanoidi | P18052 |
OMAi | QGRMGVC |
TreeFami | TF351829 |
Enzyme and pathway databases
Reactomei | R-MMU-375165, NCAM signaling for neurite out-growth R-MMU-5673001, RAF/MAP kinase cascade |
Miscellaneous databases
BioGRID-ORCSi | 19262, 1 hit in 17 CRISPR screens |
ChiTaRSi | Ptpra, mouse |
EvolutionaryTracei | P18052 |
PROi | PR:P18052 |
RNActi | P18052, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027303, Expressed in lens of camera-type eye and 304 other tissues |
ExpressionAtlasi | P18052, baseline and differential |
Genevisiblei | P18052, MM |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR016336, Tyr_Pase_rcpt_a/e-type IPR027262, Tyr_Pase_rcpt_alpha IPR000387, TYR_PHOSPHATASE_dom |
PANTHERi | PTHR19134:SF433, PTHR19134:SF433, 1 hit |
Pfami | View protein in Pfam PF00102, Y_phosphatase, 2 hits |
PIRSFi | PIRSF500808, PTPR_alpha, 1 hit PIRSF002006, PTPR_alpha_epsilon, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 2 hits SM00404, PTPc_motif, 2 hits |
SUPFAMi | SSF52799, SSF52799, 2 hits |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 2 hits PS50056, TYR_PHOSPHATASE_2, 2 hits PS50055, TYR_PHOSPHATASE_PTP, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTPRA_MOUSE | |
Accessioni | P18052Primary (citable) accession number: P18052 Secondary accession number(s): A2AHF2, Q61808 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 196 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families