UniProtKB - P17993 (UBIG_ECOLI)
Protein
Ubiquinone biosynthesis O-methyltransferase
Gene
ubiG
Organism
Escherichia coli (strain K12)
Status
Functioni
O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway.UniRule annotation1 Publication
Catalytic activityi
- a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + H+ + S-adenosyl-L-homocysteineUniRule annotation1 PublicationEC:2.1.1.64UniRule annotation1 Publication
- a 3-(all-trans-polyprenyl)benzene-1,2-diol + S-adenosyl-L-methionine = a 2-methoxy-6-(all-trans-polyprenyl)phenol + H+ + S-adenosyl-L-homocysteineUniRule annotation1 PublicationEC:2.1.1.222UniRule annotation1 Publication
: ubiquinone biosynthesis Pathwayi
This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 44 | S-adenosyl-L-methionineUniRule annotation1 Publication | 1 | |
Binding sitei | 64 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication | 1 | |
Binding sitei | 85 | S-adenosyl-L-methionineUniRule annotation1 Publication | 1 | |
Binding sitei | 129 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone methyltransferase activity Source: EcoCyc
- 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Source: InterPro
- 3-demethylubiquinol-8 3-O-methyltransferase activity Source: EcoCyc
- 3-demethylubiquinone-9 3-O-methyltransferase activity Source: UniProtKB-EC
- phosphatidylglycerol binding Source: EcoCyc
GO - Biological processi
- hyperosmotic salinity response Source: EcoCyc
- methylation Source: UniProtKB-KW
- ubiquinone biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Ubiquinone biosynthesis |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:DHHB-METHYLTRANSFER-MONOMER MetaCyc:DHHB-METHYLTRANSFER-MONOMER |
UniPathwayi | UPA00232 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquinone biosynthesis O-methyltransferaseUniRule annotationAlternative name(s): |
Gene namesi | Name:ubiGUniRule annotation Synonyms:pufX, yfaB Ordered Locus Names:b2232, JW2226 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication
GO - Cellular componenti
- cytosol Source: EcoCyc
- ubiquinone biosynthesis complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193379 | 1 – 240 | Ubiquinone biosynthesis O-methyltransferaseAdd BLAST | 240 |
Proteomic databases
jPOSTi | P17993 |
PaxDbi | P17993 |
PRIDEi | P17993 |
Interactioni
Subunit structurei
Homodimer (Probable).
Component of the Ubi complex metabolon, which regroups five ubiquinone biosynthesis proteins (UbiE, UbiF, UbiG, UbiH and UbiI) and two accessory factors (UbiK and the lipid-binding protein UbiJ) (PubMed:30686758).
Curated1 PublicationBinary interactionsi
P17993
With | #Exp. | IntAct |
---|---|---|
rplV [P61175] | 3 | EBI-559367,EBI-542255 |
Protein-protein interaction databases
BioGRIDi | 4262129, 12 interactors 850953, 7 interactors |
DIPi | DIP-11070N |
IntActi | P17993, 19 interactors |
STRINGi | 511145.b2232 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P17993 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2227, Bacteria |
HOGENOMi | CLU_042432_5_0_6 |
InParanoidi | P17993 |
PhylomeDBi | P17993 |
Family and domain databases
HAMAPi | MF_00472, UbiG, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR010233, UbiG_MeTrfase |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR01983, UbiG, 1 hit |
i Sequence
Sequence statusi: Complete.
P17993-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNAEKSPVNH NVDHEEIAKF EAVASRWWDL EGEFKPLHRI NPLRLGYIAE
60 70 80 90 100
RAGGLFGKKV LDVGCGGGIL AESMAREGAT VTGLDMGFEP LQVAKLHALE
110 120 130 140 150
SGIQVDYVQE TVEEHAAKHA GQYDVVTCME MLEHVPDPQS VVRACAQLVK
160 170 180 190 200
PGGDVFFSTL NRNGKSWLMA VVGAEYILRM VPKGTHDVKK FIKPAELLGW
210 220 230 240
VDQTSLKERH ITGLHYNPIT NTFKLGPGVD VNYMLHTQNK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00544 Genomic DNA Translation: CAA68610.1 M87509 Genomic DNA Translation: AAA24714.1 U00096 Genomic DNA Translation: AAC75292.1 AP009048 Genomic DNA Translation: BAA16049.1 |
PIRi | A47682 |
RefSeqi | NP_416735.1, NC_000913.3 WP_000990765.1, NZ_SSZK01000030.1 |
Genome annotation databases
EnsemblBacteriai | AAC75292; AAC75292; b2232 BAA16049; BAA16049; BAA16049 |
GeneIDi | 57727970 946607 |
KEGGi | ecj:JW2226 eco:b2232 |
PATRICi | fig|1411691.4.peg.3 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00544 Genomic DNA Translation: CAA68610.1 M87509 Genomic DNA Translation: AAA24714.1 U00096 Genomic DNA Translation: AAC75292.1 AP009048 Genomic DNA Translation: BAA16049.1 |
PIRi | A47682 |
RefSeqi | NP_416735.1, NC_000913.3 WP_000990765.1, NZ_SSZK01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4KDC | X-ray | 2.09 | A | 1-240 | [»] | |
4KDR | X-ray | 2.00 | A | 1-159 | [»] | |
A | 188-240 | [»] | ||||
5DPM | X-ray | 2.10 | A | 1-240 | [»] | |
SMRi | P17993 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262129, 12 interactors 850953, 7 interactors |
DIPi | DIP-11070N |
IntActi | P17993, 19 interactors |
STRINGi | 511145.b2232 |
Proteomic databases
jPOSTi | P17993 |
PaxDbi | P17993 |
PRIDEi | P17993 |
Genome annotation databases
EnsemblBacteriai | AAC75292; AAC75292; b2232 BAA16049; BAA16049; BAA16049 |
GeneIDi | 57727970 946607 |
KEGGi | ecj:JW2226 eco:b2232 |
PATRICi | fig|1411691.4.peg.3 |
Organism-specific databases
EchoBASEi | EB1133 |
Phylogenomic databases
eggNOGi | COG2227, Bacteria |
HOGENOMi | CLU_042432_5_0_6 |
InParanoidi | P17993 |
PhylomeDBi | P17993 |
Enzyme and pathway databases
UniPathwayi | UPA00232 |
BioCyci | EcoCyc:DHHB-METHYLTRANSFER-MONOMER MetaCyc:DHHB-METHYLTRANSFER-MONOMER |
Miscellaneous databases
PROi | PR:P17993 |
Family and domain databases
HAMAPi | MF_00472, UbiG, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR010233, UbiG_MeTrfase |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR01983, UbiG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBIG_ECOLI | |
Accessioni | P17993Primary (citable) accession number: P17993 Secondary accession number(s): P76924 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families