UniProtKB - P17971 (KCNAL_DROME)
Protein
Potassium voltage-gated channel protein Shal
Gene
Shal
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. May play a role in the nervous system and in the regulation of beating frequency in pacemaker cells.
Miscellaneous
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
GO - Molecular functioni
- A-type (transient outward) potassium channel activity Source: FlyBase
- voltage-gated potassium channel activity Source: GO_Central
GO - Biological processi
- associative learning Source: FlyBase
- chemical synaptic transmission Source: FlyBase
- potassium ion transmembrane transport Source: GO_Central
- potassium ion transport Source: FlyBase
- protein homooligomerization Source: InterPro
- regulation of ion transmembrane transport Source: UniProtKB-KW
Keywordsi
Molecular function | Ion channel, Potassium channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-DME-1296072, Voltage gated Potassium channels R-DME-5576894, Phase 1 - inactivation of fast Na+ channels |
Protein family/group databases
TCDBi | 1.A.1.2.3, the voltage-gated ion channel (vic) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium voltage-gated channel protein ShalAlternative name(s): Shaker cognate l Shal2 |
Gene namesi | Name:Shal Synonyms:SHAL2 ORF Names:CG9262 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0005564, Shal |
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane Source: FlyBase
- voltage-gated potassium channel complex Source: GO_Central
Other locations
- dendrite Source: UniProtKB-SubCell
- integral component of membrane Source: GO_Central
- perikaryon Source: UniProtKB-SubCell
- synapse Source: GOC
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 186 – 204 | Helical; Name=Segment S1Add BLAST | 19 | |
Transmembranei | 229 – 250 | Helical; Name=Segment S2Add BLAST | 22 | |
Transmembranei | 261 – 282 | Helical; Name=Segment S3Add BLAST | 22 | |
Transmembranei | 290 – 308 | Helical; Name=Segment S4Add BLAST | 19 | |
Transmembranei | 324 – 345 | Helical; Name=Segment S5Add BLAST | 22 | |
Transmembranei | 385 – 406 | Helical; Name=Segment S6Add BLAST | 22 |
Keywords - Cellular componenti
Cell projection, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 481 – 482 | LL → AA: Reduced binding to SIDL. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053966 | 1 – 571 | Potassium voltage-gated channel protein ShalAdd BLAST | 571 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 46 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 350 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 353 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 408 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | P17971 |
PRIDEi | P17971 |
Expressioni
Tissue specificityi
Co-expressed with SIDL in the nervous system.1 Publication
Gene expression databases
Bgeei | FBgn0005564, Expressed in central nervous system and 33 other tissues |
ExpressionAtlasi | P17971, baseline and differential |
Interactioni
Subunit structurei
Heterotetramer of potassium channel proteins (By similarity).
Interacts (via C-terminal dendritic targeting motif) with SIDL.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 65399, 4 interactors |
DIPi | DIP-19398N |
IntActi | P17971, 2 interactors |
STRINGi | 7227.FBpp0111766 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 474 – 489 | Mediates dendritic targetingBy similarityAdd BLAST | 16 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 370 – 375 | Selectivity filterBy similarity | 6 |
Domaini
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Sequence similaritiesi
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Shal sub-subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4390, Eukaryota |
GeneTreei | ENSGT00940000155343 |
InParanoidi | P17971 |
OMAi | HECLLSY |
PhylomeDBi | P17971 |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003975, K_chnl_volt-dep_Kv4 IPR024587, K_chnl_volt-dep_Kv4_C IPR021645, Shal-type_N IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF11879, DUF3399, 1 hit PF00520, Ion_trans, 1 hit PF11601, Shal-type, 1 hit |
PRINTSi | PR01491, KVCHANNEL PR01497, SHALCHANNEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform B (identifier: P17971-2) [UniParc]FASTAAdd to basket
Also known as: Shal1
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASVAAWLPF ARAAAIGWVP IATHPLPPPP MPKDRRKTDD EKLLINVSGR
60 70 80 90 100
RFETWRNTLE KYPDTLLGSN EREFFYDEDC KEYFFDRDPD IFRHILNYYR
110 120 130 140 150
TGKLHYPKHE CLTSYDEELA FFGIMPDVIG DCCYEDYRDR KRENAERLMD
160 170 180 190 200
DKLSENGDQN LQQLTNMRQK MWRAFENPHT STSALVFYYV TGFFIAVSVM
210 220 230 240 250
ANVVETVPCG HRPGRAGTLP CGERYKIVFF CLDTACVMIF TAEYLLRLFA
260 270 280 290 300
APDRCKFVRS VMSIIDVVAI MPYYIGLGIT DNDDVSGAFV TLRVFRVFRI
310 320 330 340 350
FKFSRHSQGL RILGYTLKSC ASELGFLVFS LAMAIIIFAT VMFYAEKNVN
360 370 380 390 400
GTNFTSIPAA FWYTIVTMTT LGYGDMVPET IAGKIVGGVC SLSGVLVIAL
410 420 430 440 450
PVPVIVSNFS RIYHQNQRAD KRKAQRKARL ARIRIAKASS GAAFVSKKKA
460 470 480 490 500
AEARWAAQES GIELDDNYRD EDIFELQHHH LLRCLEKTTD REFVELEIPF
510 520 530 540 550
NGQPKRPGSP SPMASPAHST NSAAGLLQSC CGRCCSQRYQ ACGKYMPAAS
560 570
NAQNSQNNQP MDGTYLVEAS F
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PFP1 | M9PFP1_DROME | Shaker cognate l, isoform C | Shal Dmel\CG9262, DNK[[v]]4, dShal, K[[v]]4, Kv4 | 520 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042874 | 490 | D → M in isoform A. 3 Publications | 1 | |
Alternative sequenceiVSP_042875 | 491 – 571 | Missing in isoform A. 3 PublicationsAdd BLAST | 81 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32660 mRNA Translation: AAA28895.1 AE014296 Genomic DNA Translation: AAF49144.2 AE014296 Genomic DNA Translation: ABW08570.1 BT024203 mRNA Translation: ABC86265.1 |
PIRi | A35312 |
RefSeqi | NP_001097646.1, NM_001104176.2 [P17971-2] NP_524159.1, NM_079435.4 [P17971-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0074973; FBpp0074741; FBgn0005564 [P17971-1] FBtr0112853; FBpp0111766; FBgn0005564 [P17971-2] |
GeneIDi | 40129 |
KEGGi | dme:Dmel_CG9262 |
UCSCi | CG9262-RB, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32660 mRNA Translation: AAA28895.1 AE014296 Genomic DNA Translation: AAF49144.2 AE014296 Genomic DNA Translation: ABW08570.1 BT024203 mRNA Translation: ABC86265.1 |
PIRi | A35312 |
RefSeqi | NP_001097646.1, NM_001104176.2 [P17971-2] NP_524159.1, NM_079435.4 [P17971-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 65399, 4 interactors |
DIPi | DIP-19398N |
IntActi | P17971, 2 interactors |
STRINGi | 7227.FBpp0111766 |
Protein family/group databases
TCDBi | 1.A.1.2.3, the voltage-gated ion channel (vic) superfamily |
Proteomic databases
PaxDbi | P17971 |
PRIDEi | P17971 |
Genome annotation databases
EnsemblMetazoai | FBtr0074973; FBpp0074741; FBgn0005564 [P17971-1] FBtr0112853; FBpp0111766; FBgn0005564 [P17971-2] |
GeneIDi | 40129 |
KEGGi | dme:Dmel_CG9262 |
UCSCi | CG9262-RB, d. melanogaster |
Organism-specific databases
CTDi | 40129 |
FlyBasei | FBgn0005564, Shal |
Phylogenomic databases
eggNOGi | KOG4390, Eukaryota |
GeneTreei | ENSGT00940000155343 |
InParanoidi | P17971 |
OMAi | HECLLSY |
PhylomeDBi | P17971 |
Enzyme and pathway databases
Reactomei | R-DME-1296072, Voltage gated Potassium channels R-DME-5576894, Phase 1 - inactivation of fast Na+ channels |
Miscellaneous databases
BioGRID-ORCSi | 40129, 0 hits in 3 CRISPR screens |
GenomeRNAii | 40129 |
PROi | PR:P17971 |
Gene expression databases
Bgeei | FBgn0005564, Expressed in central nervous system and 33 other tissues |
ExpressionAtlasi | P17971, baseline and differential |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003975, K_chnl_volt-dep_Kv4 IPR024587, K_chnl_volt-dep_Kv4_C IPR021645, Shal-type_N IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF11879, DUF3399, 1 hit PF00520, Ion_trans, 1 hit PF11601, Shal-type, 1 hit |
PRINTSi | PR01491, KVCHANNEL PR01497, SHALCHANNEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNAL_DROME | |
Accessioni | P17971Primary (citable) accession number: P17971 Secondary accession number(s): A8JNV8, Q29R37, Q9VW11 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | April 18, 2012 | |
Last modified: | December 2, 2020 | |
This is version 193 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families