murC

Functionⁱ

Cell wall formation.1 Publication

Catalytic activityⁱ

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.1 Publication

Kineticsⁱ

K_M=
100 µM for UDP-N-acetyl-alpha-D-muramate
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli."
  Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C.
  Eur. J. Biochem. 230:80-87(1995) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 1-14, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
K_M=
20 µM for L-alanine
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli."
  Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C.
  Eur. J. Biochem. 230:80-87(1995) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 1-14, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
K_M=
450 µM for ATP
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli."
  Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C.
  Eur. J. Biochem. 230:80-87(1995) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 1-14, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.

V_max=
17.3 µmol/min/mg enzyme
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli."
  Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C.
  Eur. J. Biochem. 230:80-87(1995) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 1-14, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.

pH dependenceⁱ

Optimum pH is 8.6.1 Publication

Temperature dependenceⁱ

Optimum temperature is 45 degrees Celsius.1 Publication

Pathwayⁱ: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	126 – 132	ATPSequence analysis		7

GO - Molecular functionⁱ

ATP binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 17139082
magnesium ion binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 17139082
UDP-N-acetylmuramate-L-alanine ligase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 7601127

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
cell wall organization Source: UniProtKB-KW
peptidoglycan biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 4552998
- 4563986
regulation of cell shape Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ligase
Biological process	Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:UDP-NACMUR-ALA-LIG-MONOMER MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER
BRENDAⁱ	6.3.2.8 2026
UniPathwayⁱ	UPA00219

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: UDP-N-acetylmuramate--L-alanine ligase (EC:6.3.2.8) Alternative name(s): UDP-N-acetylmuramoyl-L-alanine synthetase
Gene namesⁱ	Name:murC Ordered Locus Names:b0091, JW0089
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10619 murC

EcoGeneⁱ

EG10619 murC

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	344	G → D in murC3.		1

Chemistry databases

ChEMBLⁱ	CHEMBL5168

ChEMBLⁱ

CHEMBL5168

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000182089	1 – 491	UDP-N-acetylmuramate--L-alanine ligaseAdd BLAST		491

Proteomic databases

PaxDbⁱ	P17952
PRIDEⁱ	P17952

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
flxA	P77609	3	EBI-554607,EBI-553024
ytfT	P39328	3	EBI-554607,EBI-554774

Protein-protein interaction databases

BioGridⁱ	4261857, 473 interactors
Database of interacting proteins More...DIPⁱ	DIP-10278N
IntActⁱ	P17952, 16 interactors
STRINGⁱ	316385.ECDH10B_0073

Chemistry databases

BindingDBⁱ

P17952

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 9	Combined sources	7
Turnⁱ	10 – 12	Combined sources	3
Beta strandⁱ	21 – 26	Combined sources	6
Helixⁱ	32 – 41	Combined sources	10
Beta strandⁱ	45 – 49	Combined sources	5
Helixⁱ	55 – 62	Combined sources	8
Beta strandⁱ	66 – 70	Combined sources	5
Helixⁱ	73 – 76	Combined sources	4
Beta strandⁱ	80 – 84	Combined sources	5
Helixⁱ	93 – 100	Combined sources	8
Beta strandⁱ	105 – 107	Combined sources	3
Helixⁱ	108 – 116	Combined sources	9
Beta strandⁱ	119 – 128	Combined sources	10
Helixⁱ	130 – 143	Combined sources	14
Beta strandⁱ	149 – 151	Combined sources	3
Turnⁱ	157 – 159	Combined sources	3
Beta strandⁱ	160 – 164	Combined sources	5
Beta strandⁱ	167 – 174	Combined sources	8
Helixⁱ	181 – 184	Combined sources	4
Beta strandⁱ	188 – 192	Combined sources	5
Helixⁱ	200 – 202	Combined sources	3
Turnⁱ	203 – 205	Combined sources	3
Helixⁱ	207 – 218	Combined sources	12
Beta strandⁱ	226 – 230	Combined sources	5
Helixⁱ	234 – 239	Combined sources	6
Helixⁱ	240 – 242	Combined sources	3
Beta strandⁱ	245 – 253	Combined sources	9
Beta strandⁱ	257 – 266	Combined sources	10
Beta strandⁱ	269 – 275	Combined sources	7
Beta strandⁱ	282 – 288	Combined sources	7
Helixⁱ	291 – 307	Combined sources	17
Helixⁱ	311 – 319	Combined sources	9
Beta strandⁱ	327 – 341	Combined sources	15
Beta strandⁱ	343 – 351	Combined sources	9
Helixⁱ	356 – 367	Combined sources	12
Beta strandⁱ	372 – 379	Combined sources	8
Helixⁱ	384 – 387	Combined sources	4
Turnⁱ	388 – 390	Combined sources	3
Helixⁱ	391 – 398	Combined sources	8
Beta strandⁱ	401 – 406	Combined sources	6
Beta strandⁱ	412 – 414	Combined sources	3
Helixⁱ	422 – 425	Combined sources	4
Helixⁱ	447 – 450	Combined sources	4
Helixⁱ	451 – 453	Combined sources	3
Beta strandⁱ	456 – 463	Combined sources	8
Helixⁱ	468 – 477	Combined sources	10
Turnⁱ	478 – 480	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P17952
SMRⁱ	P17952
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P17952

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the MurCDEF family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105DFU Bacteria COG0773 LUCA
HOGENOMⁱ	HOG000256031
InParanoidⁱ	P17952
KEGG Orthology (KO) More...KOⁱ	K01924
OMAⁱ	LTMGAGD
PhylomeDBⁱ	P17952

Family and domain databases

Gene3Dⁱ	3.40.1190.10, 1 hit 3.90.190.20, 1 hit
HAMAPⁱ	MF_00046 MurC, 1 hit
InterProⁱ	View protein in InterPro IPR036565 Mur-like_cat_sf IPR004101 Mur_ligase_C IPR036615 Mur_ligase_C_dom_sf IPR013221 Mur_ligase_cen IPR000713 Mur_ligase_N IPR005758 UDP-N-AcMur_Ala_ligase_murC
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01225 Mur_ligase, 1 hit PF02875 Mur_ligase_C, 1 hit PF08245 Mur_ligase_M, 1 hit
SUPFAMⁱ	SSF53244 SSF53244, 1 hit SSF53623 SSF53623, 1 hit
TIGRFAMsⁱ	TIGR01082 murC, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P17952-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD 
        60         70         80         90        100
LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE 
       110        120        130        140        150
ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF 
       160        170        180        190        200
VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM 
       210        220        230        240        250
DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY 
       260        270        280        290        300
GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA 
       310        320        330        340        350
VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD 
       360        370        380        390        400
DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ 
       410        420        430        440        450
VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML 
       460        470        480        490 
APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH D

Length:491

Mass (Da):53,626

Last modified:August 1, 1991 - v2

Checksum:ⁱD201B35931C013FB

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X52644 Genomic DNA Translation: CAA36868.1 X55034 Genomic DNA Translation: CAA38868.1 U00096 Genomic DNA Translation: AAC73202.1 AP009048 Genomic DNA Translation: BAB96659.1 U67892 Genomic DNA Translation: AAB60787.1
PIRⁱ	JQ0545 CEECAM
NCBI Reference Sequences More...RefSeqⁱ	NP_414633.1, NC_000913.3 WP_001096049.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73202; AAC73202; b0091 BAB96659; BAB96659; BAB96659
GeneIDⁱ	946153
KEGGⁱ	ecj:JW0089 eco:b0091
PATRICⁱ	fig\|511145.12.peg.95

Protein	Similar proteins		Species	Score	Length	Source
P17952	UDP-N-acetylmuramate--L-alanine ligase		ECOBW		491	UniRef100_C4ZRI6
UDP-N-acetylmuramate--L-alanine ligase		ECODH		491
UDP-N-acetylmuramate--L-alanine ligase		ECOLC		491
UDP-N-acetylmuramate-alanine ligase		KLEPN		491
UDP-N-acetylmuramate--L-alanine ligase		ECOLX		491
+33

Protein	Similar proteins		Species	Score	Length	Source
P17952	UDP-N-acetylmuramate--L-alanine ligase		SHIDS		491	UniRef90_Q32K01
UDP-N-acetylmuramate--L-alanine ligase		KLEP7		491
UDP-N-acetylmuramate--L-alanine ligase		SALA4		491
UDP-N-acetylmuramate--L-alanine ligase		SALDC		491
UDP-N-acetylmuramate--L-alanine ligase		SALEP		491
+1056

Protein	Similar proteins		Species	Score	Length	Source
P17952	UDP-N-acetylmuramate--L-alanine ligase	)	HAEIN		475	UniRef50_P45066
UDP-N-acetylmuramate--L-alanine ligase		HAEIE		475
UDP-N-acetylmuramate--L-alanine ligase		HAEI8		475
UDP-N-acetylmuramate--L-alanine ligase		HAEIG		475
UDP-N-acetylmuramate--L-alanine ligase		PSE14		486
+5147

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X52644 Genomic DNA Translation: CAA36868.1 X55034 Genomic DNA Translation: CAA38868.1 U00096 Genomic DNA Translation: AAC73202.1 AP009048 Genomic DNA Translation: BAB96659.1 U67892 Genomic DNA Translation: AAB60787.1
PIRⁱ	JQ0545 CEECAM
RefSeqⁱ	NP_414633.1, NC_000913.3 WP_001096049.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1CC9	model	-	A	1-491	[»]
	2F00	X-ray	2.50	A/B	1-491	[»]
ProteinModelPortalⁱ	P17952
SMRⁱ	P17952
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261857, 473 interactors
DIPⁱ	DIP-10278N
IntActⁱ	P17952, 16 interactors
STRINGⁱ	316385.ECDH10B_0073

Chemistry databases

BindingDBⁱ	P17952
ChEMBLⁱ	CHEMBL5168

Proteomic databases

PaxDbⁱ	P17952
PRIDEⁱ	P17952

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73202; AAC73202; b0091 BAB96659; BAB96659; BAB96659
GeneIDⁱ	946153
KEGGⁱ	ecj:JW0089 eco:b0091
PATRICⁱ	fig\|511145.12.peg.95

Organism-specific databases

EchoBASEⁱ	EB0614
EcoGeneⁱ	EG10619 murC

Phylogenomic databases

eggNOGⁱ	ENOG4105DFU Bacteria COG0773 LUCA
HOGENOMⁱ	HOG000256031
InParanoidⁱ	P17952
KOⁱ	K01924
OMAⁱ	LTMGAGD
PhylomeDBⁱ	P17952

Enzyme and pathway databases

UniPathwayⁱ	UPA00219
BioCycⁱ	EcoCyc:UDP-NACMUR-ALA-LIG-MONOMER MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER
BRENDAⁱ	6.3.2.8 2026

Miscellaneous databases

EvolutionaryTraceⁱ	P17952
Protein Ontology More...PROⁱ	PR:P17952

Family and domain databases

Gene3Dⁱ	3.40.1190.10, 1 hit 3.90.190.20, 1 hit
HAMAPⁱ	MF_00046 MurC, 1 hit
InterProⁱ	View protein in InterPro IPR036565 Mur-like_cat_sf IPR004101 Mur_ligase_C IPR036615 Mur_ligase_C_dom_sf IPR013221 Mur_ligase_cen IPR000713 Mur_ligase_N IPR005758 UDP-N-AcMur_Ala_ligase_murC
Pfamⁱ	View protein in Pfam PF01225 Mur_ligase, 1 hit PF02875 Mur_ligase_C, 1 hit PF08245 Mur_ligase_M, 1 hit
SUPFAMⁱ	SSF53244 SSF53244, 1 hit SSF53623 SSF53623, 1 hit
TIGRFAMsⁱ	TIGR01082 murC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MURC_ECOLI
Accessionⁱ	P17952Primary (citable) accession number: P17952 Secondary accession number(s): O07099
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
	Last sequence update:	August 1, 1991
	Last modified:	March 28, 2018
	This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P17952 (MURC_ECOLI)

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UDP-N-acetylmuramate--L-alanine ligase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

pH dependencei

Temperature dependencei

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

Temperature dependenceⁱ

Pathwayⁱ: peptidoglycan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ