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UniProtKB - P17952 (MURC_ECOLI)
Protein
UDP-N-acetylmuramate--L-alanine ligase
Gene
murC
Organism
Escherichia coli (strain K12)
Status
Functioni
Cell wall formation.
1 PublicationCatalytic activityi
- ATP + L-alanine + UDP-N-acetyl-α-D-muramate = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanine1 PublicationEC:6.3.2.81 Publication
Kineticsi
- KM=100 µM for UDP-N-acetyl-alpha-D-muramate1 Publication
- KM=20 µM for L-alanine1 Publication
- KM=450 µM for ATP1 Publication
- Vmax=17.3 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 8.6.1 Publication
Temperature dependencei
Optimum temperature is 45 degrees Celsius.1 Publication
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 126 – 132 | ATPSequence analysis | 7 |
GO - Molecular functioni
- ATP binding Source: EcoliWiki
- magnesium ion binding Source: EcoliWiki
- UDP-N-acetylmuramate-L-alanine ligase activity Source: EcoliWiki
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:UDP-NACMUR-ALA-LIG-MONOMER |
BRENDAi | 6.3.2.8, 2026 |
SABIO-RKi | P17952 |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramate--L-alanine ligase (EC:6.3.2.8)Alternative name(s): UDP-N-acetylmuramoyl-L-alanine synthetase |
Gene namesi | Name:murC Ordered Locus Names:b0091, JW0089 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 344 | G → D in murC3. | 1 |
Chemistry databases
ChEMBLi | CHEMBL5168 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000182089 | 1 – 491 | UDP-N-acetylmuramate--L-alanine ligaseAdd BLAST | 491 |
Proteomic databases
jPOSTi | P17952 |
PaxDbi | P17952 |
PRIDEi | P17952 |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4261857, 473 interactors 850513, 5 interactors |
DIPi | DIP-10278N |
IntActi | P17952, 16 interactors |
STRINGi | 511145.b0091 |
Chemistry databases
BindingDBi | P17952 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P17952 |
SMRi | P17952 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17952 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MurCDEF family.Curated
Phylogenomic databases
eggNOGi | COG0773, Bacteria |
HOGENOMi | CLU_028104_2_2_6 |
InParanoidi | P17952 |
OMAi | DITYQLR |
PhylomeDBi | P17952 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_00046, MurC, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR005758, UDP-N-AcMur_Ala_ligase_MurC |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit |
TIGRFAMsi | TIGR01082, murC, 1 hit |
i Sequence
Sequence statusi: Complete.
P17952-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD
60 70 80 90 100
LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE
110 120 130 140 150
ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF
160 170 180 190 200
VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM
210 220 230 240 250
DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY
260 270 280 290 300
GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA
310 320 330 340 350
VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD
360 370 380 390 400
DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ
410 420 430 440 450
VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML
460 470 480 490
APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52644 Genomic DNA Translation: CAA36868.1 X55034 Genomic DNA Translation: CAA38868.1 U00096 Genomic DNA Translation: AAC73202.1 AP009048 Genomic DNA Translation: BAB96659.1 U67892 Genomic DNA Translation: AAB60787.1 |
PIRi | JQ0545, CEECAM |
RefSeqi | NP_414633.1, NC_000913.3 WP_001096049.1, NZ_SSZK01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC73202; AAC73202; b0091 BAB96659; BAB96659; BAB96659 |
GeneIDi | 946153 |
KEGGi | ecj:JW0089 eco:b0091 |
PATRICi | fig|511145.12.peg.95 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52644 Genomic DNA Translation: CAA36868.1 X55034 Genomic DNA Translation: CAA38868.1 U00096 Genomic DNA Translation: AAC73202.1 AP009048 Genomic DNA Translation: BAB96659.1 U67892 Genomic DNA Translation: AAB60787.1 |
PIRi | JQ0545, CEECAM |
RefSeqi | NP_414633.1, NC_000913.3 WP_001096049.1, NZ_SSZK01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2F00 | X-ray | 2.50 | A/B | 1-491 | [»] | |
AlphaFoldDBi | P17952 | |||||
SMRi | P17952 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261857, 473 interactors 850513, 5 interactors |
DIPi | DIP-10278N |
IntActi | P17952, 16 interactors |
STRINGi | 511145.b0091 |
Chemistry databases
BindingDBi | P17952 |
ChEMBLi | CHEMBL5168 |
Proteomic databases
jPOSTi | P17952 |
PaxDbi | P17952 |
PRIDEi | P17952 |
Genome annotation databases
EnsemblBacteriai | AAC73202; AAC73202; b0091 BAB96659; BAB96659; BAB96659 |
GeneIDi | 946153 |
KEGGi | ecj:JW0089 eco:b0091 |
PATRICi | fig|511145.12.peg.95 |
Organism-specific databases
EchoBASEi | EB0614 |
Phylogenomic databases
eggNOGi | COG0773, Bacteria |
HOGENOMi | CLU_028104_2_2_6 |
InParanoidi | P17952 |
OMAi | DITYQLR |
PhylomeDBi | P17952 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:UDP-NACMUR-ALA-LIG-MONOMER |
BRENDAi | 6.3.2.8, 2026 |
SABIO-RKi | P17952 |
Miscellaneous databases
EvolutionaryTracei | P17952 |
PROi | PR:P17952 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_00046, MurC, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR005758, UDP-N-AcMur_Ala_ligase_MurC |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit |
TIGRFAMsi | TIGR01082, murC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MURC_ECOLI | |
Accessioni | P17952Primary (citable) accession number: P17952 Secondary accession number(s): O07099 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | August 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families