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Protein

UDP-N-acetylmuramate--L-alanine ligase

Gene

murC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell wall formation.1 Publication

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.1 Publication

Kineticsi

  1. KM=100 µM for UDP-N-acetyl-alpha-D-muramate1 Publication
  2. KM=20 µM for L-alanine1 Publication
  3. KM=450 µM for ATP1 Publication
  1. Vmax=17.3 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.6.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 132ATPSequence analysis7

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDP-NACMUR-ALA-LIG-MONOMER
MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER
BRENDAi6.3.2.8 2026
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramate--L-alanine ligase (EC:6.3.2.8)
Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase
Gene namesi
Name:murC
Ordered Locus Names:b0091, JW0089
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10619 murC

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi344G → D in murC3. 1

Chemistry databases

ChEMBLiCHEMBL5168

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001820891 – 491UDP-N-acetylmuramate--L-alanine ligaseAdd BLAST491

Proteomic databases

PaxDbiP17952
PRIDEiP17952

Interactioni

Binary interactionsi

Protein-protein interaction databases

BioGridi4261857, 473 interactors
DIPiDIP-10278N
IntActiP17952, 16 interactors
STRINGi316385.ECDH10B_0073

Chemistry databases

BindingDBiP17952

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP17952
SMRiP17952
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17952

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.Curated

Phylogenomic databases

eggNOGiENOG4105DFU Bacteria
COG0773 LUCA
HOGENOMiHOG000256031
InParanoidiP17952
KOiK01924
OMAiLTMGAGD
PhylomeDBiP17952

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00046 MurC, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR005758 UDP-N-AcMur_Ala_ligase_murC
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
TIGRFAMsiTIGR01082 murC, 1 hit

Sequencei

Sequence statusi: Complete.

P17952-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD
60 70 80 90 100
LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE
110 120 130 140 150
ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF
160 170 180 190 200
VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM
210 220 230 240 250
DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY
260 270 280 290 300
GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA
310 320 330 340 350
VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD
360 370 380 390 400
DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ
410 420 430 440 450
VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML
460 470 480 490
APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH D
Length:491
Mass (Da):53,626
Last modified:August 1, 1991 - v2
Checksum:iD201B35931C013FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA Translation: CAA36868.1
X55034 Genomic DNA Translation: CAA38868.1
U00096 Genomic DNA Translation: AAC73202.1
AP009048 Genomic DNA Translation: BAB96659.1
U67892 Genomic DNA Translation: AAB60787.1
PIRiJQ0545 CEECAM
RefSeqiNP_414633.1, NC_000913.3
WP_001096049.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73202; AAC73202; b0091
BAB96659; BAB96659; BAB96659
GeneIDi946153
KEGGiecj:JW0089
eco:b0091
PATRICifig|511145.12.peg.95

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA Translation: CAA36868.1
X55034 Genomic DNA Translation: CAA38868.1
U00096 Genomic DNA Translation: AAC73202.1
AP009048 Genomic DNA Translation: BAB96659.1
U67892 Genomic DNA Translation: AAB60787.1
PIRiJQ0545 CEECAM
RefSeqiNP_414633.1, NC_000913.3
WP_001096049.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CC9model-A1-491[»]
2F00X-ray2.50A/B1-491[»]
ProteinModelPortaliP17952
SMRiP17952
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261857, 473 interactors
DIPiDIP-10278N
IntActiP17952, 16 interactors
STRINGi316385.ECDH10B_0073

Chemistry databases

BindingDBiP17952
ChEMBLiCHEMBL5168

Proteomic databases

PaxDbiP17952
PRIDEiP17952

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73202; AAC73202; b0091
BAB96659; BAB96659; BAB96659
GeneIDi946153
KEGGiecj:JW0089
eco:b0091
PATRICifig|511145.12.peg.95

Organism-specific databases

EchoBASEiEB0614
EcoGeneiEG10619 murC

Phylogenomic databases

eggNOGiENOG4105DFU Bacteria
COG0773 LUCA
HOGENOMiHOG000256031
InParanoidiP17952
KOiK01924
OMAiLTMGAGD
PhylomeDBiP17952

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciEcoCyc:UDP-NACMUR-ALA-LIG-MONOMER
MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER
BRENDAi6.3.2.8 2026

Miscellaneous databases

EvolutionaryTraceiP17952
PROiPR:P17952

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00046 MurC, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR005758 UDP-N-AcMur_Ala_ligase_murC
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
TIGRFAMsiTIGR01082 murC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMURC_ECOLI
AccessioniPrimary (citable) accession number: P17952
Secondary accession number(s): O07099
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 1, 1991
Last modified: March 28, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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