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UniProtKB - P17922 (SYFB_BACSU)
Protein
Phenylalanine--tRNA ligase beta subunit
Gene
pheT
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalytic activityi
- EC:6.1.1.20
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per tetramer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 462 | MagnesiumBy similarity | 1 | |
Metal bindingi | 468 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 471 | MagnesiumBy similarity | 1 | |
Metal bindingi | 472 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- phenylalanine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-KW
GO - Biological processi
- phenylalanyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU28630-MONOMER |
SABIO-RKi | P17922 |
Names & Taxonomyi
Protein namesi | Recommended name: Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)Alternative name(s): Phenylalanyl-tRNA synthetase beta subunit Short name: PheRS |
Gene namesi | Name:pheT Ordered Locus Names:BSU28630 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- phenylalanine-tRNA ligase complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126844 | 1 – 804 | Phenylalanine--tRNA ligase beta subunitAdd BLAST | 804 |
Proteomic databases
jPOSTi | P17922 |
PaxDbi | P17922 |
PRIDEi | P17922 |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta subunits.
By similarityProtein-protein interaction databases
IntActi | P17922, 1 interactor |
MINTi | P17922 |
STRINGi | 224308.BSU28630 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 155 | tRNA-bindingAdd BLAST | 116 | |
Domaini | 409 – 484 | B5Add BLAST | 76 | |
Domaini | 710 – 803 | FDX-ACBAdd BLAST | 94 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0072, Bacteria COG0073, Bacteria |
InParanoidi | P17922 |
OMAi | ISYNWLK |
PhylomeDBi | P17922 |
Family and domain databases
CDDi | cd00769, PheRS_beta_core, 1 hit cd02796, tRNA_bind_bactPheRS, 1 hit |
Gene3Di | 3.30.70.380, 1 hit 3.30.930.10, 1 hit 3.50.40.10, 1 hit |
HAMAPi | MF_00283, Phe_tRNA_synth_beta1, 1 hit |
InterProi | View protein in InterPro IPR045864, aa-tRNA-synth_II/BPL/LPL IPR005146, B3/B4_tRNA-bd IPR009061, DNA-bd_dom_put_sf IPR005121, Fdx_antiC-bd IPR036690, Fdx_antiC-bd_sf IPR012340, NA-bd_OB-fold IPR045060, Phe-tRNA-ligase_IIc_bsu IPR004532, Phe-tRNA-ligase_IIc_bsu_bact IPR020825, Phe-tRNA_synthase-like_B3/B4 IPR041616, PheRS_beta_core IPR002547, tRNA-bd_dom IPR033714, tRNA_bind_bactPheRS IPR005147, tRNA_synthase_B5-dom |
PANTHERi | PTHR10947, PTHR10947, 1 hit |
Pfami | View protein in Pfam PF03483, B3_4, 1 hit PF03484, B5, 1 hit PF03147, FDX-ACB, 1 hit PF01588, tRNA_bind, 1 hit PF17759, tRNA_synthFbeta, 1 hit |
SMARTi | View protein in SMART SM00873, B3_4, 1 hit SM00874, B5, 1 hit SM00896, FDX-ACB, 1 hit |
SUPFAMi | SSF46955, SSF46955, 1 hit SSF50249, SSF50249, 1 hit SSF54991, SSF54991, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00472, pheT_bact, 1 hit |
PROSITEi | View protein in PROSITE PS51483, B5, 1 hit PS51447, FDX_ACB, 1 hit PS50886, TRBD, 1 hit |
i Sequence
Sequence statusi: Complete.
P17922-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFVSYKWLED YVDLKGMDPA VLAEKITRAG IEVEGIEYKG EGIKGVVIGH
60 70 80 90 100
VLEREQHPNA DKLNKCLVDI GAEAPVQIIC GAPNVDKGQK VAVATVGAVL
110 120 130 140 150
PGNFKIKKAK LRGEESNGMI CSLQELGIES KLVAKEYAEG IFVFPNDAET
160 170 180 190 200
GSDALAALQL DDAILELGLT PNRADAMNML GVAYEVAAIL DTEVKLPQTD
210 220 230 240 250
YPAASEQASD YISVKIEDQE ANPLYTAKII KNVTIAPSPL WMQTKLMNAG
260 270 280 290 300
IRPHNNVVDI TNFVLLEYGQ PLHAFDYDRF GSKEVVVRKA AENEMIVTLD
310 320 330 340 350
DQERKLSADH LVITNGTKAQ AVAGVMGGAE SEVQEDTKTI LLEAAYFNGQ
360 370 380 390 400
KVRKASKDLG LRSESSVRFE KGIDPARVRL AAERAAQLIH LYAGGEVLAG
410 420 430 440 450
TVEEDHLTIE ANNIHVSADK VSSVLGLTIS KEELISIYKR LGFTVGEADD
460 470 480 490 500
LLVVTVPSRR GDITIEEDLI EEAARLYGYD NIPSTLPETA GTTGGLTPYQ
510 520 530 540 550
AKRRKVRRFL EGAGLSQAIT YSLTNEKKAT AFAIEKSLNT VLALPMSEER
560 570 580 590 600
SILRHSLVPN LLDSVSYNLA RQTDSVALYE VGSVFLTKEE DTKPVETERV
610 620 630 640 650
AGAVTGLWRK QLWQGEKKPV DFFVVKGIVE GLLDKLNVLD SIEFVQSERK
660 670 680 690 700
QLHPGRTANI LLNGSLIGFI GQVHPSLEKE LDIKETYVFE LDLHALLAAE
710 720 730 740 750
TAPLVYTAIP KYPSVTRDIA LVTDKTVTSG QLESVIKEAG GKLLKEVTVF
760 770 780 790 800
DVYEGEHMEE GKKSVAFSLQ YVNPEQTLTE EEVTKAHSKV LKALEDTYQA
VLRG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 110 – 111 | KL → NV in CAA37225 (PubMed:2127701).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53057 Genomic DNA Translation: CAA37225.1 Z75208 Genomic DNA Translation: CAA99564.1 AL009126 Genomic DNA Translation: CAB14823.1 |
PIRi | A69676, YFBSB |
RefSeqi | NP_390741.1, NC_000964.3 WP_004398979.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14823; CAB14823; BSU_28630 |
GeneIDi | 937445 |
KEGGi | bsu:BSU28630 |
PATRICi | fig|224308.179.peg.3110 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53057 Genomic DNA Translation: CAA37225.1 Z75208 Genomic DNA Translation: CAA99564.1 AL009126 Genomic DNA Translation: CAB14823.1 |
PIRi | A69676, YFBSB |
RefSeqi | NP_390741.1, NC_000964.3 WP_004398979.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P17922 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P17922, 1 interactor |
MINTi | P17922 |
STRINGi | 224308.BSU28630 |
Proteomic databases
jPOSTi | P17922 |
PaxDbi | P17922 |
PRIDEi | P17922 |
Genome annotation databases
EnsemblBacteriai | CAB14823; CAB14823; BSU_28630 |
GeneIDi | 937445 |
KEGGi | bsu:BSU28630 |
PATRICi | fig|224308.179.peg.3110 |
Phylogenomic databases
eggNOGi | COG0072, Bacteria COG0073, Bacteria |
InParanoidi | P17922 |
OMAi | ISYNWLK |
PhylomeDBi | P17922 |
Enzyme and pathway databases
BioCyci | BSUB:BSU28630-MONOMER |
SABIO-RKi | P17922 |
Family and domain databases
CDDi | cd00769, PheRS_beta_core, 1 hit cd02796, tRNA_bind_bactPheRS, 1 hit |
Gene3Di | 3.30.70.380, 1 hit 3.30.930.10, 1 hit 3.50.40.10, 1 hit |
HAMAPi | MF_00283, Phe_tRNA_synth_beta1, 1 hit |
InterProi | View protein in InterPro IPR045864, aa-tRNA-synth_II/BPL/LPL IPR005146, B3/B4_tRNA-bd IPR009061, DNA-bd_dom_put_sf IPR005121, Fdx_antiC-bd IPR036690, Fdx_antiC-bd_sf IPR012340, NA-bd_OB-fold IPR045060, Phe-tRNA-ligase_IIc_bsu IPR004532, Phe-tRNA-ligase_IIc_bsu_bact IPR020825, Phe-tRNA_synthase-like_B3/B4 IPR041616, PheRS_beta_core IPR002547, tRNA-bd_dom IPR033714, tRNA_bind_bactPheRS IPR005147, tRNA_synthase_B5-dom |
PANTHERi | PTHR10947, PTHR10947, 1 hit |
Pfami | View protein in Pfam PF03483, B3_4, 1 hit PF03484, B5, 1 hit PF03147, FDX-ACB, 1 hit PF01588, tRNA_bind, 1 hit PF17759, tRNA_synthFbeta, 1 hit |
SMARTi | View protein in SMART SM00873, B3_4, 1 hit SM00874, B5, 1 hit SM00896, FDX-ACB, 1 hit |
SUPFAMi | SSF46955, SSF46955, 1 hit SSF50249, SSF50249, 1 hit SSF54991, SSF54991, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00472, pheT_bact, 1 hit |
PROSITEi | View protein in PROSITE PS51483, B5, 1 hit PS51447, FDX_ACB, 1 hit PS50886, TRBD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYFB_BACSU | |
Accessioni | P17922Primary (citable) accession number: P17922 Secondary accession number(s): P94540 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families