UniProtKB - P17814 (4CL1_ORYSJ)
4-coumarate--CoA ligase 1
4CL1
Functioni
Involved in the phenylpropanoid metabolism by mediating the activation of a number of hydroxycinnamates for the biosynthesis of monolignols and other phenolic secondary metabolites (PubMed:21807887, PubMed:23246835).
Catalyzes the formation of CoA esters of cinnamate, 4-coumarate, caffeate and ferulate (PubMed:21807887, PubMed:23246835).
Is more efficient with substrates in the following order: ferulate > 4-coumarate > cinnamate > caffeate (PubMed:21807887).
Cannot convert sinapate to its corresponding CoA ester (PubMed:21807887, PubMed:23246835).
2 PublicationsCatalytic activityi
Kineticsi
- KM=9.4 µM for cinnamate1 Publication
- KM=11.9 µM for 4-coumarate1 Publication
- KM=29.3 µM for caffeate1 Publication
- KM=8.3 µM for ferulate1 Publication
- Vmax=100 pmol/sec/mg enzyme with cinnamate as substrate1 Publication
- Vmax=130 pmol/sec/mg enzyme with 4-coumarate as substrate1 Publication
- Vmax=140 pmol/sec/mg enzyme with caffeate as substrate1 Publication
- Vmax=110 pmol/sec/mg enzyme with ferulate as substrate1 Publication
: 3,4',5-trihydroxystilbene biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate.Curated This subpathway is part of the pathway 3,4',5-trihydroxystilbene biosynthesis, which is itself part of Phytoalexin biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate, the pathway 3,4',5-trihydroxystilbene biosynthesis and in Phytoalexin biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 257 | ATPBy similarity | 1 | |
Binding sitei | 356 | ATPBy similarity | 1 | |
Binding sitei | 440 | ATPBy similarity | 1 | |
Binding sitei | 455 | ATPBy similarity | 1 | |
Binding sitei | 547 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 209 – 217 | ATPBy similarity | 9 | |
Nucleotide bindingi | 352 – 357 | ATPBy similarity | 6 |
GO - Molecular functioni
- 4-coumarate-CoA ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- CoA-ligase activity Source: GO_Central
- trans-cinnamate-CoA ligase activity Source: UniProtKB
GO - Biological processi
- phenylpropanoid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Biological process | Phenylpropanoid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.2.1.12, 4460 |
PlantReactomei | R-OSA-1119316, Phenylpropanoid biosynthesis R-OSA-1119418, Suberin biosynthesis R-OSA-1119531, Flavonoid biosynthesis |
UniPathwayi | UPA00372;UER00547 |
Names & Taxonomyi
Protein namesi | Recommended name: 4-coumarate--CoA ligase 11 Publication (EC:6.2.1.122 Publications)Short name: 4CL 11 Publication Short name: Os4CL11 Publication Alternative name(s): 4-coumaroyl-CoA synthase 1Curated |
Gene namesi | Name:4CL11 Publication Synonyms:4CLCurated Ordered Locus Names:Os08g0245200Imported, LOC_Os08g14760Curated ORF Names:OJ1033_B09.16Imported |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
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PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193031 | 1 – 564 | 4-coumarate--CoA ligase 1Add BLAST | 564 |
Proteomic databases
PaxDbi | P17814 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Genevisiblei | P17814, OS |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 282 – 351 | SBD1By similarityAdd BLAST | 70 | |
Regioni | 352 – 419 | SBD2By similarityAdd BLAST | 68 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1176, Eukaryota |
HOGENOMi | CLU_000022_59_2_1 |
InParanoidi | P17814 |
OMAi | AHMRLFI |
OrthoDBi | 683933at2759 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV
60 70 80 90 100
AARPCLIDGA TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN
110 120 130 140 150
SPEFVLSFFA ASRVGAAVTT ANPMSTPHEI ESQLAAAGAT VVITESMAAD
160 170 180 190 200
KLPSHSHGAL TVVLIDERRD GCLHFWDDLM SEDEASPLAG DEDDEKVFDP
210 220 230 240 250
DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN IGLHAGDVIL
260 270 280 290 300
CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL
310 320 330 340 350
VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG
360 370 380 390 400
QGYGMTEAGP VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL
410 420 430 440 450
GRNLPGEICI RGQQIMKGYL NNPEATKNTI DAEGWLHTGD IGYVDDDDEI
460 470 480 490 500
FIVDRLKEII KYRGFQVAPA ELEALLITHP SIADAAVVGK QIEPEIGEIP
510 520 530 540 550
VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI PKAPSGKILR
560
KELRKQLQHL QQEA
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 405 | P → R in CAA36850 (PubMed:2235510).Curated | 1 | |
Sequence conflicti | 477 | I → N in CAA36850 (PubMed:2235510).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52623 Genomic DNA Translation: CAA36850.1 Sequence problems. AP003859 Genomic DNA Translation: BAD05189.1 Different initiation. AP008214 Genomic DNA Translation: BAF23267.1 AP014964 Genomic DNA Translation: BAT04523.1 AK069932 mRNA Translation: BAG91685.1 |
PIRi | JU0311 |
RefSeqi | XP_015650724.1, XM_015795238.1 |
Genome annotation databases
EnsemblPlantsi | Os08t0245200-01; Os08t0245200-01; Os08g0245200 |
GeneIDi | 4345054 |
Gramenei | Os08t0245200-01; Os08t0245200-01; Os08g0245200 |
KEGGi | osa:4345054 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52623 Genomic DNA Translation: CAA36850.1 Sequence problems. AP003859 Genomic DNA Translation: BAD05189.1 Different initiation. AP008214 Genomic DNA Translation: BAF23267.1 AP014964 Genomic DNA Translation: BAT04523.1 AK069932 mRNA Translation: BAG91685.1 |
PIRi | JU0311 |
RefSeqi | XP_015650724.1, XM_015795238.1 |
3D structure databases
SMRi | P17814 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS08T0245200-01 |
Proteomic databases
PaxDbi | P17814 |
Genome annotation databases
EnsemblPlantsi | Os08t0245200-01; Os08t0245200-01; Os08g0245200 |
GeneIDi | 4345054 |
Gramenei | Os08t0245200-01; Os08t0245200-01; Os08g0245200 |
KEGGi | osa:4345054 |
Phylogenomic databases
eggNOGi | KOG1176, Eukaryota |
HOGENOMi | CLU_000022_59_2_1 |
InParanoidi | P17814 |
OMAi | AHMRLFI |
OrthoDBi | 683933at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00372;UER00547 |
BRENDAi | 6.2.1.12, 4460 |
PlantReactomei | R-OSA-1119316, Phenylpropanoid biosynthesis R-OSA-1119418, Suberin biosynthesis R-OSA-1119531, Flavonoid biosynthesis |
Gene expression databases
Genevisiblei | P17814, OS |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 4CL1_ORYSJ | |
Accessioni | P17814Primary (citable) accession number: P17814 Secondary accession number(s): B7EH38, Q0J6Z8, Q6ZKV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | September 27, 2005 | |
Last modified: | February 23, 2022 | |
This is version 133 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families