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Protein

Adenine DNA glycosylase

Gene

mutY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine: 7,8-dihydro-8-oxoguanine (8-oxo-dGTP).2 Publications

Catalytic activityi

Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.2 Publications

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei37Proton donor/acceptorBy similarity1
Sitei138Transition state stabilizerBy similarity1
Metal bindingi192Iron-sulfur (4Fe-4S)1
Metal bindingi199Iron-sulfur (4Fe-4S)1
Metal bindingi202Iron-sulfur (4Fe-4S)1
Metal bindingi208Iron-sulfur (4Fe-4S)1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10627-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine DNA glycosylase (EC:3.2.2.312 Publications)
Alternative name(s):
A/G-specific adenine glycosylase
Gene namesi
Name:mutY
Synonyms:micA
Ordered Locus Names:b2961, JW2928
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10627 mutY

Pathology & Biotechi

Chemistry databases

DrugBankiDB00173 Adenine
DB02379 Beta-D-Glucose
DB04077 Glycerol

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022341 – 350Adenine DNA glycosylaseAdd BLAST350

Proteomic databases

PaxDbiP17802
PRIDEiP17802

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262359, 411 interactors
851767, 3 interactors
DIPiDIP-10289N
IntActiP17802, 11 interactors
STRINGi316385.ECDH10B_3136

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 17Combined sources15
Helixi23 – 25Combined sources3
Helixi30 – 40Combined sources11
Beta strandi41 – 43Combined sources3
Helixi45 – 58Combined sources14
Helixi62 – 67Combined sources6
Helixi70 – 77Combined sources8
Helixi84 – 99Combined sources16
Helixi108 – 112Combined sources5
Helixi119 – 130Combined sources12
Helixi139 – 149Combined sources11
Helixi158 – 171Combined sources14
Helixi177 – 190Combined sources14
Beta strandi194 – 196Combined sources3
Helixi199 – 201Combined sources3
Turni203 – 207Combined sources5
Helixi209 – 213Combined sources5
Helixi216 – 218Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KG2X-ray1.20A1-225[»]
1KG3X-ray1.55A1-225[»]
1KG4X-ray1.60A1-225[»]
1KG5X-ray1.35A1-225[»]
1KG6X-ray1.50A1-225[»]
1KG7X-ray1.50A1-225[»]
1KQJX-ray1.70A1-225[»]
1MUDX-ray1.80A1-225[»]
1MUNX-ray1.20A1-225[»]
1MUYX-ray1.40A1-225[»]
1WEFX-ray1.90A1-225[»]
1WEGX-ray1.80A1-225[»]
1WEIX-ray1.45A1-225[»]
ProteinModelPortaliP17802
SMRiP17802
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17802

Family & Domainsi

Sequence similaritiesi

Belongs to the Nth/MutY family.Curated

Phylogenomic databases

eggNOGiENOG4105CEN Bacteria
COG1194 LUCA
HOGENOMiHOG000028744
InParanoidiP17802
KOiK03575
OMAiRDPYRVW
PhylomeDBiP17802

Family and domain databases

CDDicd03431 DNA_Glycosylase_C, 1 hit
cd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
InterProiView protein in InterPro
IPR005760 A/G_AdeGlyc_MutY
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR029119 MutY_C
IPR015797 NUDIX_hydrolase-like_dom_sf
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
PF14815 NUDIX_4, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
SSF55811 SSF55811, 1 hit
TIGRFAMsiTIGR01084 mutY, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

P17802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQASQFSAQV LDWYDKYGRK TLPWQIDKTP YKVWLSEVML QQTQVATVIP
60 70 80 90 100
YFERFMARFP TVTDLANAPL DEVLHLWTGL GYYARARNLH KAAQQVATLH
110 120 130 140 150
GGKFPETFEE VAALPGVGRS TAGAILSLSL GKHFPILDGN VKRVLARCYA
160 170 180 190 200
VSGWPGKKEV ENKLWSLSEQ VTPAVGVERF NQAMMDLGAM ICTRSKPKCS
210 220 230 240 250
LCPLQNGCIA AANNSWALYP GKKPKQTLPE RTGYFLLLQH EDEVLLAQRP
260 270 280 290 300
PSGLWGGLYC FPQFADEESL RQWLAQRQIA ADNLTQLTAF RHTFSHFHLD
310 320 330 340 350
IVPMWLPVSS FTGCMDEGNA LWYNLAQPPS VGLAAPVERL LQQLRTGAPV
Length:350
Mass (Da):39,149
Last modified:August 1, 1990 - v1
Checksum:iC7D3657C03EBBF4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52391 Genomic DNA Translation: CAA36624.1
M59471 Genomic DNA Translation: AAA72957.1
U28377 Genomic DNA Translation: AAA69128.1
U00096 Genomic DNA Translation: AAC75998.1
AP009048 Genomic DNA Translation: BAE77024.1
PIRiB38535
RefSeqiNP_417436.1, NC_000913.3
WP_001321419.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75998; AAC75998; b2961
BAE77024; BAE77024; BAE77024
GeneIDi947447
KEGGiecj:JW2928
eco:b2961
PATRICifig|1411691.4.peg.3770

Similar proteinsi

Entry informationi

Entry nameiMUTY_ECOLI
AccessioniPrimary (citable) accession number: P17802
Secondary accession number(s): Q2M9N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 25, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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