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Protein

Elongation factor Tu, chloroplastic

Gene

TUFA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 93GTPBy similarity8
Nucleotide bindingi148 – 152GTPBy similarity5
Nucleotide bindingi203 – 206GTPBy similarity4

GO - Molecular functioni

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, chloroplastic
Short name:
EF-Tu
Alternative name(s):
Ras-related protein RABE1b
Short name:
AtRABE1b
Ras-related protein Rab8D
Short name:
AtRab8D
Gene namesi
Name:TUFA
Synonyms:RAB8D, RABE1B
Ordered Locus Names:At4g20360
ORF Names:F9F13.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G20360
TAIRilocus:2128615 AT4G20360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 67ChloroplastSequence analysisAdd BLAST67
ChainiPRO_000000745468 – 476Elongation factor Tu, chloroplasticAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei94PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17745
PRIDEiP17745

2D gel databases

SWISS-2DPAGEiP17745

PTM databases

iPTMnetiP17745

Expressioni

Gene expression databases

ExpressionAtlasiP17745 baseline and differential
GenevisibleiP17745 AT

Interactioni

Subunit structurei

Interacts with PI5K2.1 Publication

Protein-protein interaction databases

BioGridi13075, 11 interactors
IntActiP17745, 1 interactor
MINTiP17745
STRINGi3702.AT4G20360.1

Structurei

3D structure databases

ProteinModelPortaliP17745
SMRiP17745
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 281tr-type GAdd BLAST205

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 93G1By similarity8
Regioni127 – 131G2By similarity5
Regioni148 – 151G3By similarity4
Regioni203 – 206G4By similarity4
Regioni241 – 243G5By similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0460 Eukaryota
COG0050 LUCA
HOGENOMiHOG000229290
InParanoidiP17745
KOiK02358
OMAiNIKMVVN
OrthoDBiEOG09360CEA
PhylomeDBiP17745

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISAPAACS SSSRILCSYS SPSPSLCPAI STSGKLKTLT LSSSFLPSYS
60 70 80 90 100
LTTTSASQST RRSFTVRAAR GKFERKKPHV NIGTIGHVDH GKTTLTAALT
110 120 130 140 150
MALASIGSSV AKKYDEIDAA PEERARGITI NTATVEYETE NRHYAHVDCP
160 170 180 190 200
GHADYVKNMI TGAAQMDGAI LVVSGADGPM PQTKEHILLA KQVGVPDMVV
210 220 230 240 250
FLNKEDQVDD AELLELVELE VRELLSSYEF NGDDIPIISG SALLAVETLT
260 270 280 290 300
ENPKVKRGDN KWVDKIYELM DAVDDYIPIP QRQTELPFLL AVEDVFSITG
310 320 330 340 350
RGTVATGRVE RGTVKVGETV DLVGLRETRS YTVTGVEMFQ KILDEALAGD
360 370 380 390 400
NVGLLLRGIQ KADIQRGMVL AKPGSITPHT KFEAIIYVLK KEEGGRHSPF
410 420 430 440 450
FAGYRPQFYM RTTDVTGKVT KIMNDKDEES KMVMPGDRVK IVVELIVPVA
460 470
CEQGMRFAIR EGGKTVGAGV IGTILE
Length:476
Mass (Da):51,630
Last modified:August 1, 1990 - v1
Checksum:i6041F540224A1738
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → P in AAN31832 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52256 Genomic DNA Translation: CAA36498.1
AL080253 Genomic DNA Translation: CAB45802.2
AL161552 Genomic DNA Translation: CAB79036.1
CP002687 Genomic DNA Translation: AEE84313.1
CP002687 Genomic DNA Translation: ANM67987.1
AF410329 mRNA Translation: AAK95315.1
AF419609 mRNA Translation: AAL31941.1
AY074355 mRNA Translation: AAL67051.1
BT000687 mRNA Translation: AAN31832.1
BT000699 mRNA Translation: AAN31843.1
BT000998 mRNA Translation: AAN41398.1
BT002642 mRNA Translation: AAO11558.1
PIRiS09152
RefSeqiNP_001329775.1, NM_001341435.1
NP_193769.1, NM_118155.2
UniGeneiAt.24137
At.71279
At.71947
At.75368

Genome annotation databases

EnsemblPlantsiAT4G20360.1; AT4G20360.1; AT4G20360
AT4G20360.2; AT4G20360.2; AT4G20360
GeneIDi827784
GrameneiAT4G20360.1; AT4G20360.1; AT4G20360
AT4G20360.2; AT4G20360.2; AT4G20360
KEGGiath:AT4G20360

Similar proteinsi

Entry informationi

Entry nameiEFTU_ARATH
AccessioniPrimary (citable) accession number: P17745
Secondary accession number(s): Q8GTE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 25, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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