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UniProtKB - P17728 (SCXA_LEIHE)

Entry version 121 (22 Apr 2020)
Sequence version 2 (01 Mar 1992)
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Protein

Alpha-insect toxin LqhaIT

Gene
N/A
Organism
Leiurus hebraeus (Deathstalker scorpion) (Leiurus quinquestriatus hebraeus)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The dissociation is voltage-dependent. This toxin is active on insects. It is also highly toxic to crustaceans and has a measurable but low toxicity to mice.

Miscellaneous

The binding sites for this contractive toxin differ from those shared by the excitatory and depressant insect toxins.

Caution

The mutagenesis studies described in PubMed:8756487 and PubMed:9169449 are made with the sequence from PubMed:2383565 (see the sequence conflict in position 83-85).Curated

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha-insect toxin LqhaIT
Alternative name(s):
Lqh-alpha-IT
Short name:
Alpha-IT
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiLeiurus hebraeus (Deathstalker scorpion) (Leiurus quinquestriatus hebraeus)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri6884 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Leiurus ›

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the lethal dose (LD), paralytic dose (PD), effect dose (ED) or lethal concentration (LC) of a protein toxin.<p><a href='/help/toxic_dose' target='_top'>More...</a></p>Toxic dosei

LD50 is 250 µg/kg by subcutaneous injection to mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi27K → A: 74% loss of toxicity and 39.4-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi27K → D: 99.4% loss of toxicity and 1611-fold decrease in affinity to cockroach neuronal receptor. 95% loss of toxicity and 71.4-fold decrease in affinity to cockroach neuronal receptor; when associated with A-34. 99.6% loss of toxicity and 21303-fold decrease in affinity to cockroach neuronal receptor; when associated with D-28 and V-29. 1 Publication1
Mutagenesisi28N → D: 99.6% loss of toxicity and 21303-fold decrease in affinity to cockroach neuronal receptor; when associated with D-27 and V-29. 1
Mutagenesisi29Y → S: 90% loss of toxicity and 114.3-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi29Y → V: 99.6% loss of toxicity and 21303-fold decrease in affinity to cockroach neuronal receptor; when associated with D-27 and D-28. 1 Publication1
Mutagenesisi29Y → W: 57% loss of toxicity and 3.7-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi34E → A: 77% loss of toxicity and 0.6-fold decrease in affinity to cockroach neuronal receptor; 95% loss of toxicity and 71.4-fold decrease in affinity to cockroach neuronal receptor; when associated with D-27. 1 Publication1
Mutagenesisi36F → G: 86% loss of toxicity and 160-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi37R → A: 78% loss of toxicity and 231.4-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi37R → K: 59% loss of toxicity and 0.46-fold decrease in affinity to cockroach neuronal receptor; when associated with N-38. 1 Publication1
Mutagenesisi38D → H: 75% loss of toxicity and 6-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi38D → N: 15% increase in toxicity and 1.2-fold decrease in affinity to cockroach neuronal receptor; 59% loss of toxicity and 0.46-fold decrease in affinity to cockroach neuronal receptor; when associated with K-37. 1 Publication1
Mutagenesisi59G → S: 36% loss of toxicity and 25.7-fold decrease in affinity to cockroach neuronal receptor; when associated with P-60. 1
Mutagenesisi60K → P: 36% loss of toxicity and 25.7-fold decrease in affinity to cockroach neuronal receptor; when associated with S-59. 1
Mutagenesisi68Y → I: 20% loss of toxicity to mice and 1.18-fold decrease in affinity to locust synaptosomal membranes; when associated with K-69. 540% loss of toxicity to mice and 1.08-fold decrease in affinity to locust synaptosomal membranes; when associated with K-69 and K-73. 1 Publication1
Mutagenesisi69A → K: 20% loss of toxicity to mice and 1.18-fold decrease in affinity to locust synaptosomal membranes; when associated with I-68. 540% loss of toxicity to mice and 1.08-fold decrease in affinity to locust synaptosomal membranes; when associated with I-68 and K-73. 1 Publication1
Mutagenesisi73N → K: 540% loss of toxicity to mice and 1.08-fold decrease in affinity to locust synaptosomal membranes; when associated with I-68 and K-69. 1 Publication1
Mutagenesisi81K → L: 81% loss of toxicity and 220-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi84R → A: 47% decrease in toxicity and 54.3-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi84R → D: 95% decrease in toxicity and 2394-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi84R → H: 220% decrease in toxicity and 0.3-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1
Mutagenesisi84R → N: 6% increase in toxicity and 58.6-fold decrease in affinity to cockroach neuronal receptor. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 191 PublicationAdd BLAST19
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003529620 – 85Alpha-insect toxin LqhaITAdd BLAST66

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi31 ↔ 82PROSITE-ProRule annotation1 Publication
Disulfide bondi35 ↔ 55PROSITE-ProRule annotation1 Publication
Disulfide bondi41 ↔ 65PROSITE-ProRule annotation1 Publication
Disulfide bondi45 ↔ 67PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRoteomics IDEntifications database

More...PRIDEi		P17728

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed by the venom gland.

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P17728

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P17728

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini21 – 83LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST63

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (beta/alpha/beta/beta).Curated

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

Conserved Domains Database

More...CDDi		cd00107 Knot1, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.30.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR003614 Scorpion_toxin-like
IPR036574 Scorpion_toxin-like_sf
IPR018218 Scorpion_toxinL
IPR002061 Scorpion_toxinL/defensin

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00537 Toxin_3, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00285 SCORPNTOXIN

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00505 Knot1, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF57095 SSF57095, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51863 LCN_CSAB, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P17728-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNHLVMISLA LLLLLGVESV RDAYIAKNYN CVYECFRDAY CNELCTKNGA 
        60         70         80 
SSGYCQWAGK YGNACWCYAL PDNVPIRVPG KCHRK
Length:85
Mass (Da):9,571
Last modified:March 1, 1992 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3338BED532D53FA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti83 – 85HRK → R AA sequence (PubMed:2383565).Curated3

Sequence databases

Protein sequence database of the Protein Information Resource

More...PIRi		A39306

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

PIRiA39306

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LQHNMR-A20-82[»]
1LQINMR-A20-82[»]
2ASCX-ray1.10A20-82[»]
2ATBX-ray1.60A/B20-82[»]
2YEOX-ray1.08A20-82[»]
SMRiP17728
ModBaseiSearch...
PDBe-KBiSearch...

Proteomic databases

PRIDEiP17728

Miscellaneous databases

EvolutionaryTraceiP17728

Family and domain databases

CDDicd00107 Knot1, 1 hit
Gene3Di3.30.30.10, 1 hit
InterProiView protein in InterPro
IPR003614 Scorpion_toxin-like
IPR036574 Scorpion_toxin-like_sf
IPR018218 Scorpion_toxinL
IPR002061 Scorpion_toxinL/defensin
PfamiView protein in Pfam
PF00537 Toxin_3, 1 hit
PRINTSiPR00285 SCORPNTOXIN
SMARTiView protein in SMART
SM00505 Knot1, 1 hit
SUPFAMiSSF57095 SSF57095, 1 hit
PROSITEiView protein in PROSITE
PS51863 LCN_CSAB, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSCXA_LEIHE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P17728
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: March 1, 1992
Last modified: April 22, 2020
This is version 121 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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