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UniProtKB - P17679 (GATA1_MOUSE)

Protein

Erythroid transcription factor

Gene

Gata1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (By similarity).By similarity5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 228GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri258 – 282GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Erythroid transcription factor
Alternative name(s):
Eryf1
GATA-binding factor 1
Short name:
GATA-1
Short name:
GF-1
NF-E1 DNA-binding protein
Gene namesi
Name:Gata1
Synonyms:Gf-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95661 Gata1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi49S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi72S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi137K → R: Abolishes sumoylation. No change in PIAS4 binding nor on transcriptional activity. 1 Publication1
Mutagenesisi142S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi178S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi187S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi203E → V: Disrupts interaction with ZFPM1. Binds normally to DNA. 1 Publication1
Mutagenesisi204C → R: Disrupts interaction with ZFPM1 and binding to DNA. 1 Publication1
Mutagenesisi205V → G: Disrupts interaction with ZFPM1. Binds normally to DNA. 1 Publication1
Mutagenesisi205V → M: Disrupts interaction with ZFPM1. Binds normally to DNA. 1 Publication1
Mutagenesisi207C → G, R or W: Disrupts interaction with ZFPM1. 2 Publications1
Mutagenesisi207C → P: Stability of binding to DNA reduced. 2 Publications1
Mutagenesisi208G → E or V: Disrupts interaction with ZFPM1 and binding to DNA. 1 Publication1
Mutagenesisi218D → G or V: No effect on interaction with ZFPM1. 1 Publication1
Mutagenesisi222H → R: Disrupts interaction with ZFPM1. Binds normally to DNA. 1 Publication1
Mutagenesisi224L → P: Disrupts interaction with ZFPM1 and binding to DNA. 1 Publication1
Mutagenesisi225C → R, S or Y: Disrupts interaction with ZFPM1. 1 Publication1
Mutagenesisi228C → R or S: Disrupts interaction with ZFPM1. 1 Publication1
Mutagenesisi230L → F: Stability of binding to DNA reduced. 1 Publication1
Mutagenesisi233K → E: No effect on interaction with ZFPM1. 1 Publication1
Mutagenesisi245 – 246KK → AA: No effect on DNA binding. Reduces acetylation. Reduces ability to induce erythroid differentiation. Abrogates acetylation; when associated with 312-A--A-316. Abrogates ability to induce erythroid differentiation; when associated with 312-A--A-316. Reduces binding to CREBBP; when associated with 312-A--A-316. Disrupts stable association with chromatin; when associated with 312-A--A-316. 2 Publications2
Mutagenesisi245 – 246KK → RR: No effect on DNA binding. 2 Publications2
Mutagenesisi261C → P: Abolishes DNA-binding. 1 Publication1
Mutagenesisi284L → F: Binds to DNA with reduced affinity. 1 Publication1
Mutagenesisi310S → A: Loss of phosphorylation of the chymotryptic peptide. 1 Publication1
Mutagenesisi312 – 316KGKKK → AGAAA: No effect on DNA binding. Reduces acetylation. Reduces binding to CREBBP. Reduces ability to induce erythroid differentiation. Abrogates acetylation; when associated with 245-A-A-246. Abrogates ability to induce erythroid differentiation; when associated with 245-A-A-246. Reduces binding to CREBBP; when associated with 245-A-A-246. Disrupts stable association with chromatin; when associated with 245-A-A-246. 2 Publications5
Mutagenesisi312 – 316KGKKK → RGRRR: No effect on DNA binding. 2 Publications5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833981 – 413Erythroid transcription factorAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26Phosphoserine1 Publication1
Modified residuei49Phosphoserine1 Publication1
Modified residuei72Phosphoserine1 Publication1
Cross-linki137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Modified residuei142Phosphoserine1 Publication1
Modified residuei178Phosphoserine1 Publication1
Modified residuei187Phosphoserine1 Publication1
Modified residuei233N6-acetyllysine; by EP300By similarity1
Modified residuei245N6-acetyllysine; by EP300By similarity1
Modified residuei246N6-acetyllysine; by CREBBP1 Publication1
Modified residuei246N6-acetyllysine; by EP300By similarity1
Modified residuei252N6-acetyllysine; by CREBBP1 Publication1
Modified residuei308N6-acetyllysine1 Publication1
Modified residuei310Phosphoserine1 Publication1
Modified residuei312N6-acetyllysine; by CREBBP2 Publications1
Modified residuei314N6-acetyllysine1 Publication1
Modified residuei315N6-acetyllysine1 Publication1

Post-translational modificationi

Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).By similarity
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity.2 Publications
Acetylated on Lys-233, Lys-245 Lys-246 by EP300 (By similarity). Acetylated on Lys-246, Lys-252 and Lys-312 by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo.By similarity2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP17679
PRIDEiP17679

PTM databases

iPTMnetiP17679
PhosphoSitePlusiP17679

Expressioni

Tissue specificityi

Erythrocytes. Expressed (at protein level) in liver.2 Publications

Developmental stagei

Detected at 11.5-day fetal livers (at protein level). Isoform 2 detected earlier at 8.5-day embryo.1 Publication

Gene expression databases

BgeeiENSMUSG00000031162 Expressed in 118 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExiMM_GATA1
ExpressionAtlasiP17679 baseline and differential
GenevisibleiP17679 MM

Interactioni

Subunit structurei

May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1 (By similarity). Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with BRD3. Interacts with MED1, CCAR1 and CALCOCO1. Interacts with EP300 (By similarity). Interacts with CEBPE (By similarity).By similarity8 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi199838, 23 interactors
CORUMiP17679
DIPiDIP-40883N
IntActiP17679, 9 interactors
MINTiP17679
STRINGi10090.ENSMUSP00000033502

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP17679
SMRiP17679
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17679

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni200 – 330Interaction with MED1 and CCAR11 PublicationAdd BLAST131
Regioni203 – 222Required for interaction with ZFPM1By similarityAdd BLAST20
Regioni249 – 315Interaction with CALCOCO11 PublicationAdd BLAST67

Domaini

The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 228GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri258 – 282GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
GeneTreeiENSGT00760000119221
HOGENOMiHOG000047701
HOVERGENiHBG051705
InParanoidiP17679
KOiK09182
OMAiYSKTGLY
OrthoDBiEOG091G0AUR
PhylomeDBiP17679
TreeFamiTF315391

Family and domain databases

CDDicd00202 ZnF_GATA, 2 hits
Gene3Di3.30.50.10, 2 hits
InterProiView protein in InterPro
IPR029524 GATA-1
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PTHR10071:SF190 PTHR10071:SF190, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 2 hits
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 2 hits
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 2 hits
PS50114 GATA_ZN_FINGER_2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P17679-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDFPGLGALG TSEPLPQFVD SALVSSPSDS TGFFSSGPEG LDAASSSTSP 
        60         70         80         90        100
NAATAAASAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGG SPYASWAYGK 
       110        120        130        140        150
TALYPASTVC PSHEDAPSQA LEDQEGKSNN TFLDTLKTER LSPDLLTLGT 
       160        170        180        190        200
ALPASLPVTG SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE 
       210        220        230        240        250
ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV 
       260        270        280        290        300
SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYFKLHQ VNRPLTMRKD 
       310        320        330        340        350
GIQTRNRKAS GKGKKKRGSN LAGAGAAEGP AGGFMVVAGS SSSGNCGEVA 
       360        370        380        390        400
SGLALGTAGT AHLYQGLGPV VLSGPVSHLM PFPGPLLGSP TTSFPTGPAP 
       410 
TTSSTSVIAP LSS
Length:413
Mass (Da):42,674
Last modified:August 1, 1990 - v1
Checksum:iBB627A92700D557A
GO
Isoform 2 (identifier: P17679-2) [UniParc]FASTAAdd to basket
Also known as: GATA-1s

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Note: Produced by alternative initiation at Met-84 of isoform 1. Less effective than isoform 1 in its ability to transactivate target genes.
Show »
Length:330
Mass (Da):34,188
Checksum:i8BF1F251EB8E47A1
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AUB3B1AUB3_MOUSE
Erythroid transcription factor
Gata1
109Annotation score:
B1AUB4B1AUB4_MOUSE
Erythroid transcription factor
Gata1
124Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29D → G in AAH52653 (PubMed:15489334).Curated1
Sequence conflicti129N → S in AAH52653 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0414521 – 83Missing in isoform 2. CuratedAdd BLAST83

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15763 mRNA Translation: CAA33769.1
AK146915 mRNA Translation: BAE27527.1
AL670169 Genomic DNA Translation: CAM17247.1
CH466638 Genomic DNA Translation: EDL33938.1
X57530 Genomic DNA Translation: CAA40751.1
BC052653 mRNA Translation: AAH52653.1
CCDSiCCDS29981.1 [P17679-1]
PIRiS04655
RefSeqiNP_032115.1, NM_008089.2 [P17679-1]
XP_011245750.1, XM_011247448.2 [P17679-1]
UniGeneiMm.335973

Genome annotation databases

EnsembliENSMUST00000033502; ENSMUSP00000033502; ENSMUSG00000031162 [P17679-1]
GeneIDi14460
KEGGimmu:14460
UCSCiuc009snl.2 mouse [P17679-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15763 mRNA Translation: CAA33769.1
AK146915 mRNA Translation: BAE27527.1
AL670169 Genomic DNA Translation: CAM17247.1
CH466638 Genomic DNA Translation: EDL33938.1
X57530 Genomic DNA Translation: CAA40751.1
BC052653 mRNA Translation: AAH52653.1
CCDSiCCDS29981.1 [P17679-1]
PIRiS04655
RefSeqiNP_032115.1, NM_008089.2 [P17679-1]
XP_011245750.1, XM_011247448.2 [P17679-1]
UniGeneiMm.335973

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNFNMR-A200-243[»]
1Y0JNMR-A200-243[»]
2L5ENMR-B308-320[»]
2L6YNMR-A200-238[»]
2L6ZNMR-A200-238[»]
3VD6X-ray1.98C200-318[»]
3VEKX-ray2.63C/F200-318[»]
ProteinModelPortaliP17679
SMRiP17679
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199838, 23 interactors
CORUMiP17679
DIPiDIP-40883N
IntActiP17679, 9 interactors
MINTiP17679
STRINGi10090.ENSMUSP00000033502

PTM databases

iPTMnetiP17679
PhosphoSitePlusiP17679

Proteomic databases

PaxDbiP17679
PRIDEiP17679

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033502; ENSMUSP00000033502; ENSMUSG00000031162 [P17679-1]
GeneIDi14460
KEGGimmu:14460
UCSCiuc009snl.2 mouse [P17679-1]

Organism-specific databases

CTDi2623
MGIiMGI:95661 Gata1

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
GeneTreeiENSGT00760000119221
HOGENOMiHOG000047701
HOVERGENiHBG051705
InParanoidiP17679
KOiK09182
OMAiYSKTGLY
OrthoDBiEOG091G0AUR
PhylomeDBiP17679
TreeFamiTF315391

Enzyme and pathway databases

ReactomeiR-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

EvolutionaryTraceiP17679
PROiPR:P17679
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031162 Expressed in 118 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExiMM_GATA1
ExpressionAtlasiP17679 baseline and differential
GenevisibleiP17679 MM

Family and domain databases

CDDicd00202 ZnF_GATA, 2 hits
Gene3Di3.30.50.10, 2 hits
InterProiView protein in InterPro
IPR029524 GATA-1
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PTHR10071:SF190 PTHR10071:SF190, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 2 hits
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 2 hits
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 2 hits
PS50114 GATA_ZN_FINGER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGATA1_MOUSE
AccessioniPrimary (citable) accession number: P17679
Secondary accession number(s): Q3UIH9, Q7TMX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 7, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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