UniProtKB - P17659 (KCNA6_RAT)
Protein
Potassium voltage-gated channel subfamily A member 6
Gene
Kcna6
Organism
Rattus norvegicus (Rat)
Status
Functioni
Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KNCA5, KCNA6, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:1993474, PubMed:15618540). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (Probable). Homotetrameric channels display rapid activation and slow inactivation (PubMed:1993474).Curated2 Publications
GO - Molecular functioni
- delayed rectifier potassium channel activity Source: UniProtKB
- voltage-gated potassium channel activity Source: GO_Central
GO - Biological processi
- potassium ion transmembrane transport Source: UniProtKB
- protein homooligomerization Source: InterPro
- regulation of ion transmembrane transport Source: UniProtKB-KW
Keywordsi
Molecular function | Ion channel, Potassium channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296072, Voltage gated Potassium channels |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium voltage-gated channel subfamily A member 6Alternative name(s): RCK2 Voltage-gated potassium channel subunit Kv1.6 Voltage-gated potassium channel subunit Kv2 |
Gene namesi | Name:Kcna6 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 62083, Kcna6 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Curated
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- voltage-gated potassium channel complex Source: UniProtKB
Other locations
- axon Source: RGD
- axon terminus Source: RGD
- integral component of membrane Source: GO_Central
- potassium channel complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 172 – 193 | Helical; Name=Segment S1By similarityAdd BLAST | 22 | |
Transmembranei | 264 – 285 | Helical; Name=Segment S2By similarityAdd BLAST | 22 | |
Transmembranei | 297 – 317 | Helical; Name=Segment S3By similarityAdd BLAST | 21 | |
Transmembranei | 339 – 359 | Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST | 21 | |
Transmembranei | 375 – 396 | Helical; Name=Segment S5By similarityAdd BLAST | 22 | |
Intramembranei | 411 – 422 | Helical; Name=Pore helixBy similarityAdd BLAST | 12 | |
Intramembranei | 423 – 430 | By similarity | 8 | |
Transmembranei | 438 – 466 | Helical; Name=Segment S6By similarityAdd BLAST | 29 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053992 | 1 – 530 | Potassium voltage-gated channel subfamily A member 6Add BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | Phosphoserine; by CK2Sequence analysis | 1 | |
Lipidationi | 286 | S-palmitoyl cysteineSequence analysis | 1 | |
Modified residuei | 512 | Phosphoserine; by PKACurated | 1 | |
Modified residuei | 528 | Phosphothreonine; by PKASequence analysis | 1 |
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P17659 |
PRIDEi | P17659 |
PTM databases
iPTMneti | P17659 |
PhosphoSitePlusi | P17659 |
Interactioni
Subunit structurei
Homotetramer and heterotetramer of potassium channel proteins (Probable).
Interacts with KCNAB1 and KCNAB2 (PubMed:9334400).
Curated1 PublicationProtein-protein interaction databases
CORUMi | P17659 |
STRINGi | 10116.ENSRNOP00000067348 |
Chemistry databases
BindingDBi | P17659 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 361 – 374 | S4-S5 linkerBy similarityAdd BLAST | 14 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 423 – 428 | Selectivity filterBy similarity | 6 | |
Motifi | 527 – 529 | PDZ-bindingSequence analysis | 3 |
Domaini
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity
Sequence similaritiesi
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1545, Eukaryota |
InParanoidi | P17659 |
PhylomeDBi | P17659 |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003972, K_chnl_volt-dep_Kv1 IPR004053, KCNA6 IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl |
PANTHERi | PTHR11537, PTHR11537, 1 hit PTHR11537:SF104, PTHR11537:SF104, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF00520, Ion_trans, 1 hit |
PRINTSi | PR01513, KV16CHANNEL PR01491, KVCHANNEL PR01496, SHAKERCHANEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P17659-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRSEKSLTLA APGEVRGPEG EQQDAGEFQE AEGGGGCCSS ERLVINISGL
60 70 80 90 100
RYETQLRTLS LFPDTLLGDP GRRVRFFDPL RNEYFFDRNR PSFDAILYYY
110 120 130 140 150
QSGGRLRRPV NVPLDIFMEE IRFYQLGDEA LAAFREDEGC LPEGGEDEKP
160 170 180 190 200
LPSQPFQRQV WLLFEYPESS GPARGIAIVS VLVILISIVI FCLETLPQFR
210 220 230 240 250
ADGRGGSNEG SGTRMSPASR GSHEEEDEDE DSYAFPGSIP SGGLGTGGTS
260 270 280 290 300
SFSTLGGSFF TDPFFLVETL CIVWFTFELL VRFSACPSKA AFFRNIMNII
310 320 330 340 350
DLVAIFPYFI TLGTELVQRH EQQPVSGGSG QNRQQAMSLA ILRVIRLVRV
360 370 380 390 400
FRIFKLSRHS KGLQILGKTL QASMRELGLL IFFLFIGVIL FSSAVYFAEA
410 420 430 440 450
DDVDSLFPSI PDAFWWAVVT MTTVGYGDMY PMTVGGKIVG SLCAIAGVLT
460 470 480 490 500
IALPVPVIVS NFNYFYHRET EQEEQGQYTH VTCGQPTPDL KATDNGLGKP
510 520 530
DFAEASRERR SSYLPTPHRA YAEKRMLTEV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8L6 | G3V8L6_RAT | Potassium voltage-gated channel sub... | Kcna6 rCG_30127 | 530 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 241 | S → L no nucleotide entry (PubMed:1993474).Curated | 1 | |
Sequence conflicti | 333 | R → G in AAA41499 (PubMed:2361015).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17621 mRNA Translation: CAA35622.1 M27159 Genomic DNA Translation: AAA41499.1 Sequence problems. |
PIRi | JH0167 |
Genome annotation databases
UCSCi | RGD:62083, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17621 mRNA Translation: CAA35622.1 M27159 Genomic DNA Translation: AAA41499.1 Sequence problems. |
PIRi | JH0167 |
3D structure databases
SMRi | P17659 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | P17659 |
STRINGi | 10116.ENSRNOP00000067348 |
Chemistry databases
BindingDBi | P17659 |
ChEMBLi | CHEMBL4105906 |
GuidetoPHARMACOLOGYi | 543 |
PTM databases
iPTMneti | P17659 |
PhosphoSitePlusi | P17659 |
Proteomic databases
PaxDbi | P17659 |
PRIDEi | P17659 |
Protocols and materials databases
ABCDi | P17659, 1 sequenced antibody |
Genome annotation databases
UCSCi | RGD:62083, rat |
Organism-specific databases
RGDi | 62083, Kcna6 |
Phylogenomic databases
eggNOGi | KOG1545, Eukaryota |
InParanoidi | P17659 |
PhylomeDBi | P17659 |
Enzyme and pathway databases
Reactomei | R-RNO-1296072, Voltage gated Potassium channels |
Miscellaneous databases
PROi | PR:P17659 |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003972, K_chnl_volt-dep_Kv1 IPR004053, KCNA6 IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl |
PANTHERi | PTHR11537, PTHR11537, 1 hit PTHR11537:SF104, PTHR11537:SF104, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF00520, Ion_trans, 1 hit |
PRINTSi | PR01513, KV16CHANNEL PR01491, KVCHANNEL PR01496, SHAKERCHANEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNA6_RAT | |
Accessioni | P17659Primary (citable) accession number: P17659 Secondary accession number(s): P19025 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families