UniProtKB - P17631 (DNAJ_BACSU)
Chaperone protein DnaJ
dnaJ
Functioni
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.
UniRule annotationCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 145 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 148 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 162 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 165 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 188 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 191 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 202 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 205 | Zinc 1UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 132 – 214 | CR-typeUniRule annotationAdd BLAST | 83 |
GO - Molecular functioni
- ATP binding Source: InterPro
- heat shock protein binding Source: InterPro
- unfolded protein binding Source: GO_Central
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- chaperone cofactor-dependent protein refolding Source: GO_Central
- DNA replication Source: UniProtKB-KW
- protein refolding Source: GO_Central
- response to heat Source: InterPro
Keywordsi
Molecular function | Chaperone |
Biological process | DNA replication, Stress response |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU25460-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Chaperone protein DnaJUniRule annotation |
Gene namesi | Name:dnaJUniRule annotation Ordered Locus Names:BSU25460 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070727 | 1 – 375 | Chaperone protein DnaJAdd BLAST | 375 |
Proteomic databases
jPOSTi | P17631 |
PRIDEi | P17631 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- heat shock protein binding Source: InterPro
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
IntActi | P17631, 2 interactors |
STRINGi | 224308.BSU25460 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 69 | JUniRule annotationAdd BLAST | 65 | |
Repeati | 145 – 152 | CXXCXGXG motif | 8 | |
Repeati | 162 – 169 | CXXCXGXG motif | 8 | |
Repeati | 188 – 195 | CXXCXGXG motif | 8 | |
Repeati | 202 – 209 | CXXCXGXG motif | 8 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 132 – 214 | CR-typeUniRule annotationAdd BLAST | 83 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | COG0484, Bacteria |
InParanoidi | P17631 |
OMAi | SDIMDAF |
PhylomeDBi | P17631 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit cd10719, DnaJ_zf, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
HAMAPi | MF_01152, DnaJ, 1 hit |
InterProi | View protein in InterPro IPR012724, DnaJ IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR018253, DnaJ_domain_CS IPR008971, HSP40/DnaJ_pept-bd IPR001305, HSP_DnaJ_Cys-rich_dom IPR036410, HSP_DnaJ_Cys-rich_dom_sf IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit PF00684, DnaJ_CXXCXGXG, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits SSF57938, SSF57938, 1 hit |
TIGRFAMsi | TIGR02349, DnaJ_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00636, DNAJ_1, 1 hit PS50076, DNAJ_2, 1 hit PS51188, ZF_CR, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKRDYYEVL GVSKSASKDE IKKAYRKLSK KYHPDINKEA GSDEKFKEVK
60 70 80 90 100
EAYETLSDDQ KRAHYDQFGH TDPNQGFGGG GFGGGDFGGF GFDDIFSSIF
110 120 130 140 150
GGGTRRRDPN APRQGADLQY TMTLSFEDAA FGKETTIEIP REETCETCKG
160 170 180 190 200
SGAKPGTNPE TCSHCGGSGQ LNVEQNTPFG KVVNRRVCHH CEGTGKIIKN
210 220 230 240 250
KCADCGGKGK IKKRKKINVT IPAGVDDGQQ LRLSGQGEPG INGGPAGDLF
260 270 280 290 300
VVFHVRAHEF FERDGDDIYC EMPLTFAQAA LGDEVEVPTL HGKVKLKIPA
310 320 330 340 350
GTQTGTKFRL RGKGVQNVRG YGQGDQHIVV RVVTPTNLTD KQKDIIREFA
360 370
EVSGNLPDEQ EMSFFDKVKR AFKGD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 110 – 114 | NAPRQ → KLRAR in AAA22529 (PubMed:1339421).Curated | 5 | |
Sequence conflicti | 110 – 114 | NAPRQ → KLRAR in BAA12465 (PubMed:8969508).Curated | 5 | |
Sequence conflicti | 245 – 247 | PAG → LP in AAA22529 (PubMed:1339421).Curated | 3 | |
Sequence conflicti | 245 – 247 | PAG → LP in BAA12465 (PubMed:8969508).Curated | 3 | |
Sequence conflicti | 296 – 297 | Missing in AAA22529 (PubMed:1339421).Curated | 2 | |
Sequence conflicti | 296 – 297 | Missing in BAA12465 (PubMed:8969508).Curated | 2 | |
Sequence conflicti | 296 – 297 | Missing in BAA12077 (PubMed:9023197).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84964 Genomic DNA Translation: AAA22529.1 D84432 Genomic DNA Translation: BAA12465.1 AL009126 Genomic DNA Translation: CAB14488.2 X52064 Genomic DNA Translation: CAA36287.1 D83717 Genomic DNA Translation: BAA12077.1 |
PIRi | B41874 |
RefSeqi | NP_390424.2, NC_000964.3 WP_003230010.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14488; CAB14488; BSU_25460 |
GeneIDi | 937848 |
KEGGi | bsu:BSU25460 |
PATRICi | fig|224308.179.peg.2767 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84964 Genomic DNA Translation: AAA22529.1 D84432 Genomic DNA Translation: BAA12465.1 AL009126 Genomic DNA Translation: CAB14488.2 X52064 Genomic DNA Translation: CAA36287.1 D83717 Genomic DNA Translation: BAA12077.1 |
PIRi | B41874 |
RefSeqi | NP_390424.2, NC_000964.3 WP_003230010.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P17631 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P17631, 2 interactors |
STRINGi | 224308.BSU25460 |
Proteomic databases
jPOSTi | P17631 |
PRIDEi | P17631 |
Genome annotation databases
EnsemblBacteriai | CAB14488; CAB14488; BSU_25460 |
GeneIDi | 937848 |
KEGGi | bsu:BSU25460 |
PATRICi | fig|224308.179.peg.2767 |
Phylogenomic databases
eggNOGi | COG0484, Bacteria |
InParanoidi | P17631 |
OMAi | SDIMDAF |
PhylomeDBi | P17631 |
Enzyme and pathway databases
BioCyci | BSUB:BSU25460-MONOMER |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit cd10719, DnaJ_zf, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
HAMAPi | MF_01152, DnaJ, 1 hit |
InterProi | View protein in InterPro IPR012724, DnaJ IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR018253, DnaJ_domain_CS IPR008971, HSP40/DnaJ_pept-bd IPR001305, HSP_DnaJ_Cys-rich_dom IPR036410, HSP_DnaJ_Cys-rich_dom_sf IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit PF00684, DnaJ_CXXCXGXG, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits SSF57938, SSF57938, 1 hit |
TIGRFAMsi | TIGR02349, DnaJ_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00636, DNAJ_1, 1 hit PS50076, DNAJ_2, 1 hit PS51188, ZF_CR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DNAJ_BACSU | |
Accessioni | P17631Primary (citable) accession number: P17631 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | July 7, 2009 | |
Last modified: | June 2, 2021 | |
This is version 152 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families