UniProtKB - P17584 (DHD2_LACPA)
Protein
D-2-hydroxyisocaproate dehydrogenase
Gene
N/A
Organism
Lactobacillus paracasei
Status
Functioni
Catalyzes the NAD dependent reversible, stereospecific interconversion between 2-ketocarboxylic acids and D-2-hydroxy-carboxylic acids.
Miscellaneous
Can be applied in an industrial process for the production of D-amino acid.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 175 | NADCombined sources1 Publication | 1 | |
Binding sitei | 205 | NAD; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 211 | NADCombined sources1 Publication | 1 | |
Active sitei | 234 | By similarity | 1 | |
Binding sitei | 258 | NADCombined sources1 Publication | 1 | |
Active sitei | 263 | By similarity | 1 | |
Active sitei | 295 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 155 – 156 | NADCombined sources1 Publication | 2 | |
Nucleotide bindingi | 205 – 206 | NADBy similarity | 2 | |
Nucleotide bindingi | 232 – 234 | NADCombined sources1 Publication | 3 |
GO - Molecular functioni
- NAD binding Source: InterPro
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Names & Taxonomyi
Protein namesi | |
Organismi | Lactobacillus paracasei |
Taxonomic identifieri | 1597 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075940 | 1 – 333 | D-2-hydroxyisocaproate dehydrogenaseAdd BLAST | 333 |
Proteomic databases
PRIDEi | P17584 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P17584 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17584 |
Family & Domainsi
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR006139 D-isomer_2_OHA_DH_cat_dom IPR029753 D-isomer_DH_CS IPR029752 D-isomer_DH_CS1 IPR006140 D-isomer_DH_NAD-bd IPR036291 NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389 2-Hacid_dh, 1 hit PF02826 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065 D_2_HYDROXYACID_DH_1, 1 hit PS00670 D_2_HYDROXYACID_DH_2, 1 hit PS00671 D_2_HYDROXYACID_DH_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P17584-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIIAYGARV DEIQYFKQWA KDTGNTLEYH TEFLDENTVE WAKGFDGINS
60 70 80 90 100
LQTTPYAAGV FEKMHAYGIK FLTIRNVGTD NIDMTAMKQY GIRLSNVPAY
110 120 130 140 150
SPAAIAEFAL TDTLYLLRNM GKVQAQLQAG DYEKAGTFIG KELGQQTVGV
160 170 180 190 200
MGTGHIGQVA IKLFKGFGAK VIAYDPYPMK GDHPDFDYVS LEDLFKQSDV
210 220 230 240 250
IDLHVPGIEQ NTHIINEAAF NLMKPGAIVI NTARPNLIDT QAMLSNLKSG
260 270 280 290 300
KLAGVGIDTY EYETEDLLNL AKHGSFKDPL WDELLGMPNV VLSPHIAYYT
310 320 330
ETAVHNMVYF SLQHLVDFLT KGETSTEVTG PAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 322 – 333 | GETST…TGPAK → FKPARKLLVQQVVN (PubMed:2504649).CuratedAdd BLAST | 12 |
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26929 Genomic DNA Translation: AAA25236.1 |
PIRi | JU0050 DELBC |
RefSeqi | WP_003577354.1, NZ_QVHW01000030.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26929 Genomic DNA Translation: AAA25236.1 |
PIRi | JU0050 DELBC |
RefSeqi | WP_003577354.1, NZ_QVHW01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DXY | X-ray | 1.86 | A | 1-333 | [»] | |
SMRi | P17584 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB03229 2-Oxo-4-Methylpentanoic Acid |
Proteomic databases
PRIDEi | P17584 |
Miscellaneous databases
EvolutionaryTracei | P17584 |
Family and domain databases
InterProi | View protein in InterPro IPR006139 D-isomer_2_OHA_DH_cat_dom IPR029753 D-isomer_DH_CS IPR029752 D-isomer_DH_CS1 IPR006140 D-isomer_DH_NAD-bd IPR036291 NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389 2-Hacid_dh, 1 hit PF02826 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065 D_2_HYDROXYACID_DH_1, 1 hit PS00670 D_2_HYDROXYACID_DH_2, 1 hit PS00671 D_2_HYDROXYACID_DH_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHD2_LACPA | |
Accessioni | P17584Primary (citable) accession number: P17584 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 8, 2019 | |
This is version 106 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references