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Protein

Nitrate reductase [NADH]

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADNote: Binds 1 FAD.
  • hemeNote: Binds 1 heme group. The heme group is called cytochrome b-557.
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi307Iron (heme axial ligand)PROSITE-ProRule annotation1
Sitei593Necessary for efficient electron Transfer1

GO - Molecular functioni

  • FAD binding Source: GO_Central
  • heme binding Source: InterPro
  • molybdenum ion binding Source: InterPro
  • nitrate reductase (NADH) activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BRENDAi1.7.1.1 6752

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi25899

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi593C → S: Reduction of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000166062‹1 – 621Nitrate reductase [NADH]Add BLAST›621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi138InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP17571
PRIDEiP17571

PTM databases

iPTMnetiP17571

Expressioni

Gene expression databases

ExpressionAtlasiP17571 baseline and differential

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi4577.GRMZM2G568636_P01

Structurei

Secondary structure

1621
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP17571
SMRiP17571
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17571

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini249 – 324Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini361 – 473FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
KOG0535 Eukaryota
KOG0537 Eukaryota
COG0543 LUCA
COG2041 LUCA

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.40.50.80, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR008333 Cbr1-like_FAD-bd_dom
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 FAD-bd_FR_type
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR039261 FNR_nucleotide-bd
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
SSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Fragment.

P17571-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
GFPVRVIIPG CIGGRMVKWL KRIIVTPAES DNYYHFKDNR VLPSHVDAEL
60 70 80 90 100
ANAEAWWYKP EYIINELNIN SVITTPCHDE ILPINAFTTQ RPYTLKGYAY
110 120 130 140 150
SGGGKKVTRV EVTLDGGETW LVCTDHPEKP TKYGKYWCWC FWSLEVEVLD
160 170 180 190 200
LLSAKEIAVR AWDESLNTQP EKLIWNVMGM MNNCWFRVKT NVCKPHKGEI
210 220 230 240 250
GIVFDHPTLP GNESGGWMAK EKHLETAEAA APGLKRSTST PFMNTTDVGK
260 270 280 290 300
EFTMSEVRKH ASQESAWIVV HGHVYDCTKF LKDHPGGADS ILINAGTDCT
310 320 330 340 350
EEFDAIHSDK AKALLDTYRI GELITTGTGY SSDNSVHGGS VLSHLAPIRR
360 370 380 390 400
AVRAPALSNP REKIHCRLVG KKELSRDVRL FRFSLPSPDQ VLGLPIGKHI
410 420 430 440 450
FVCASIEGKL CMRAYTPTSM VDEIGHFDLL VKVYFKNEHP KFPNGGLMTQ
460 470 480 490 500
YLDSLPVGSY IDVKGPLGHV EYTGRGSFVI NGKQRHASRL AMICGGSGIT
510 520 530 540 550
PMYQIIQAVL RDQPEDHTEM HLVYANRTED DILLRDELDR WAAEYPDRLK
560 570 580 590 600
VWYVIDQVKR PEEGWKYSVG FVTEAVLREH VPEGGDDTLA LACGPPPMIQ
610 620
FAISPNLEKM KYDMANSFVV F
Length:621
Mass (Da):69,773
Last modified:February 1, 1995 - v2
Checksum:iACC276E4ED0FF5D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27821 mRNA Translation: AAA03202.1 Sequence problems.
PIRiS19254
UniGeneiZm.15815
Zm.1826

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27821 mRNA Translation: AAA03202.1 Sequence problems.
PIRiS19254
UniGeneiZm.15815
Zm.1826

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CNEX-ray3.00A352-621[»]
1CNFX-ray2.70A352-621[»]
2CNDX-ray2.50A352-621[»]
ProteinModelPortaliP17571
SMRiP17571
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G568636_P01

PTM databases

iPTMnetiP17571

Proteomic databases

PaxDbiP17571
PRIDEiP17571

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MaizeGDBi25899

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
KOG0535 Eukaryota
KOG0537 Eukaryota
COG0543 LUCA
COG2041 LUCA

Enzyme and pathway databases

BRENDAi1.7.1.1 6752

Miscellaneous databases

EvolutionaryTraceiP17571

Gene expression databases

ExpressionAtlasiP17571 baseline and differential

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.40.50.80, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR008333 Cbr1-like_FAD-bd_dom
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 FAD-bd_FR_type
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR039261 FNR_nucleotide-bd
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
SSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNIA1_MAIZE
AccessioniPrimary (citable) accession number: P17571
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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