Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrate reductase [NADH]

Gene

NIA

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi188MolybdenumBy similarity1
Metal bindingi571Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi594Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:NIA
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660611 – 911Nitrate reductase [NADH]Add BLAST911

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi427InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP17570

Expressioni

Gene expression databases

ExpressionAtlasiP17570 baseline and differential

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi4081.Solyc11g013810.1.1

Structurei

3D structure databases

ProteinModelPortaliP17570
SMRiP17570
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini536 – 611Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini654 – 766FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
KOG0535 Eukaryota
KOG0537 Eukaryota
COG0543 LUCA
COG2041 LUCA
COG5274 LUCA
InParanoidiP17570

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.40.50.80, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR008333 Cbr1-like_FAD-bd_dom
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 FAD-bd_FR_type
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR039261 FNR_nucleotide-bd
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
SSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P17570-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAASVENRQY THLEPGLSGV GRTFKPRPDS PVRGCNFPPS SNHELPFQKK
60 70 80 90 100
QNPPIYLDYS SSEDEDDDDE KNEYVQMIKK GKTELEPSIH DTRDEGTADN
110 120 130 140 150
WIERNFSLIR LTGKHPFNSE PPLSRLMHHG FITPVPLHYV RNHGPVPKAS
160 170 180 190 200
WSDWTVEVTG LVKRPMKFTM DQLVNEFPSR EFPVTLVCAG NRRKEQNMVK
210 220 230 240 250
QTIGFNWGAA AVSTTVWRGV PLRALLKRCG VQSKKKGALN VCFEGSDVLP
260 270 280 290 300
GGGGSKYGTS IKKEFAMDPS RDIIVAYMQN GEMLSPDHGF PVRMIIPGFI
310 320 330 340 350
GGRMVKWLKR IVVTTQESES YYHYKDNRVL PPHVDAELAN AEAWWYKPEY
360 370 380 390 400
IINELNINSV ITTPCHEEIL PINAWTTQRP YTLRGYAYSG GGKKVTRVEV
410 420 430 440 450
TLDGGETWSV CTLDHPEKPT KYGKYWCWCF WSLEVEVLDL LSAKEIAVRA
460 470 480 490 500
TDETLNTQPE KLIWNVMGMM NNCWFRVKMN VCKPHKGEIG IVFEHPTQPG
510 520 530 540 550
NQSGGWMAKE RHLEISAVAP PTLKKSISTP FMNTASKMYS MSEVRKHNSS
560 570 580 590 600
DSAWIIVHGH IYDASRFLKD HPGGVDSILI NAGTDCTEEF DAIHSDKAKK
610 620 630 640 650
LLEDFRIGEL ITTGYTSDSS PNSSVHGSSS ISSFLAPIKE LVQTPTRSVA
660 670 680 690 700
LIPREKIPCK LVDKQSISHD VRKFKFALPS EDQVLGLPVG KHIFLCATVD
710 720 730 740 750
DKLCMRAYTP TSTVDEVGFF ELVVKIYFKG VHPKFPNGGQ MSQHLDSLPI
760 770 780 790 800
GAFLDVKGPL GHIEYQGKGN FLVHGKQKFA KKLAMIAGGT GITPVYQVMQ
810 820 830 840 850
SILKDPEDDT EMYVVYANRT EDDILLKDEL DAWAEQVPNR VKVWYVVQES
860 870 880 890 900
ITQGWKYSTG FVTESILREH IPEPSHTTLA LACGPPPMIQ FAINPNLEKM
910
GYDIKEELLV F
Length:911
Mass (Da):102,453
Last modified:August 1, 1990 - v1
Checksum:iDE8736181F02A0D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14060 Genomic DNA Translation: CAA32218.1
PIRiJQ0373 RDTONH
UniGeneiLes.7286

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14060 Genomic DNA Translation: CAA32218.1
PIRiJQ0373 RDTONH
UniGeneiLes.7286

3D structure databases

ProteinModelPortaliP17570
SMRiP17570
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc11g013810.1.1

Proteomic databases

PaxDbiP17570

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
KOG0535 Eukaryota
KOG0537 Eukaryota
COG0543 LUCA
COG2041 LUCA
COG5274 LUCA
InParanoidiP17570

Gene expression databases

ExpressionAtlasiP17570 baseline and differential

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.40.50.80, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR008333 Cbr1-like_FAD-bd_dom
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 FAD-bd_FR_type
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR039261 FNR_nucleotide-bd
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
SSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNIA_SOLLC
AccessioniPrimary (citable) accession number: P17570
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again