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Protein

Proto-oncogene Wnt-3

Gene

Wnt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (By similarity). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PubMed:10431240). Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development (PubMed:12569130).By similarityCurated2 Publications

Miscellaneous

Some mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named Wnt3.1 Publication

GO - Molecular functioni

  • frizzled binding Source: MGI
  • protein domain specific binding Source: UniProtKB
  • receptor ligand activity Source: MGI
  • signaling receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene Wnt-3
Alternative name(s):
Proto-oncogene Int-4
Gene namesi
Name:Wnt3
Synonyms:Int-4, Wnt-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98955 Wnt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004141722 – 355Proto-oncogene Wnt-3Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi80 ↔ 91By similarity
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi131 ↔ 139By similarity
Disulfide bondi141 ↔ 158By similarity
Disulfide bondi206 ↔ 220By similarity
Disulfide bondi208 ↔ 215By similarity
Lipidationi212O-palmitoleoyl serine; by PORCNBy similarity1
Disulfide bondi300 ↔ 315By similarity
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi330 ↔ 345By similarity
Disulfide bondi332 ↔ 342By similarity
Disulfide bondi337 ↔ 338By similarity

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

MaxQBiP17553
PaxDbiP17553
PRIDEiP17553

PTM databases

PhosphoSitePlusiP17553

Expressioni

Tissue specificityi

Detected at low levels in adult brain (PubMed:2162045). Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.1 Publication

Developmental stagei

Detected at 6.25 to 7.5 dpc in primitive streak, proximal epiblast, visceral endoderm and at the junction between the embryonic and extraembryonic ectoderm, with higher levels in the posterior region (PubMed:10431240, PubMed:19841259). Detected in the ectoderm at forelimb buds at 9.5 dpc (PubMed:12569130). Detected in all outgrowing limbs and in ectoderm flanking the limbs at least till 11.5 dpc (PubMed:12569130). Highly expressed in embryos at 11.5 and 12.5 dpc, with very low expression levels before and after this period (PubMed:2162045).4 Publications

Gene expression databases

BgeeiENSMUSG00000000125 Expressed in 82 organ(s), highest expression level in brain
CleanExiMM_WNT3
ExpressionAtlasiP17553 baseline and differential
GenevisibleiP17553 MM

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids (By similarity). Interacts with PORCN (PubMed:10866835). Interacts with WLS (PubMed:19841259).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KlO350824EBI-1570853,EBI-1570828

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204574, 3 interactors
DIPiDIP-39897N
IntActiP17553, 2 interactors
STRINGi10090.ENSMUSP00000000127

Structurei

3D structure databases

ProteinModelPortaliP17553
SMRiP17553
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiP17553
KOiK00312
OMAiMCGCDSH
OrthoDBiEOG091G0OFF
PhylomeDBiP17553
TreeFamiTF105310

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR009141 Wnt3
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01843 WNT3PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17553-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPHLLGLLL GLLLSGTRVL AGYPIWWSLA LGQQYTSLAS QPLLCGSIPG
60 70 80 90 100
LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI
110 120 130 140 150
FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG
160 170 180 190 200
EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD
210 220 230 240 250
HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR
260 270 280 290 300
ESRGWVETLR AKYALFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC
310 320 330 340 350
NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCVFHWCCYV SCQECIRIYD

VHTCK
Length:355
Mass (Da):39,659
Last modified:August 1, 1990 - v1
Checksum:iF31CFD65E43E9C17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32502 mRNA Translation: AAB38109.1
CCDSiCCDS25523.1
PIRiA35503
RefSeqiNP_033547.1, NM_009521.2
UniGeneiMm.159091

Genome annotation databases

EnsembliENSMUST00000000127; ENSMUSP00000000127; ENSMUSG00000000125
GeneIDi22415
KEGGimmu:22415
UCSCiuc007lvs.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32502 mRNA Translation: AAB38109.1
CCDSiCCDS25523.1
PIRiA35503
RefSeqiNP_033547.1, NM_009521.2
UniGeneiMm.159091

3D structure databases

ProteinModelPortaliP17553
SMRiP17553
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204574, 3 interactors
DIPiDIP-39897N
IntActiP17553, 2 interactors
STRINGi10090.ENSMUSP00000000127

PTM databases

PhosphoSitePlusiP17553

Proteomic databases

MaxQBiP17553
PaxDbiP17553
PRIDEiP17553

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000127; ENSMUSP00000000127; ENSMUSG00000000125
GeneIDi22415
KEGGimmu:22415
UCSCiuc007lvs.2 mouse

Organism-specific databases

CTDi7473
MGIiMGI:98955 Wnt3

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiP17553
KOiK00312
OMAiMCGCDSH
OrthoDBiEOG091G0OFF
PhylomeDBiP17553
TreeFamiTF105310

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking

Miscellaneous databases

PROiPR:P17553
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000125 Expressed in 82 organ(s), highest expression level in brain
CleanExiMM_WNT3
ExpressionAtlasiP17553 baseline and differential
GenevisibleiP17553 MM

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR009141 Wnt3
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01843 WNT3PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWNT3_MOUSE
AccessioniPrimary (citable) accession number: P17553
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 12, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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