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UniProtKB - P17505 (MDHM_YEAST)
Protein
Malate dehydrogenase, mitochondrial
Gene
MDH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 28100 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.1.1.37PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 50 | NADBy similarity | 1 | |
Binding sitei | 99 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 105 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 112 | NADBy similarity | 1 | |
Binding sitei | 137 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 171 | SubstratePROSITE-ProRule annotation | 1 | |
Active sitei | 195 | Proton acceptorBy similarity | 1 | |
Binding sitei | 245 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 24 – 30 | NADBy similarity | 7 | |
Nucleotide bindingi | 135 – 137 | NADBy similarity | 3 |
GO - Molecular functioni
- L-malate dehydrogenase activity Source: SGD
- mRNA binding Source: SGD
GO - Biological processi
- aerobic respiration Source: SGD
- malate metabolic process Source: InterPro
- tricarboxylic acid cycle Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Tricarboxylic acid cycle |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-SCE-70263, Gluconeogenesis R-SCE-71403, Citric acid cycle (TCA cycle) |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MDH1 Ordered Locus Names:YKL085W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001568, MDH1 |
VEuPathDBi | FungiDB:YKL085W |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Mitochondrion
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 17 | Mitochondrion2 PublicationsAdd BLAST | 17 | |
ChainiPRO_0000018633 | 18 – 334 | Malate dehydrogenase, mitochondrialAdd BLAST | 317 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 177 | PhosphoserineCombined sources | 1 | |
Modified residuei | 199 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P17505 |
PaxDbi | P17505 |
PRIDEi | P17505 |
TopDownProteomicsi | P17505 |
2D gel databases
UCD-2DPAGEi | P17505 |
PTM databases
iPTMneti | P17505 |
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
P17505
With | #Exp. | IntAct |
---|---|---|
SSA2 [P10592] | 2 | EBI-10594,EBI-8603 |
Protein-protein interaction databases
BioGRIDi | 34048, 134 interactors |
DIPi | DIP-5734N |
IntActi | P17505, 31 interactors |
STRINGi | 4932.YKL085W |
Miscellaneous databases
RNActi | P17505, protein |
Structurei
3D structure databases
AlphaFoldDBi | P17505 |
SMRi | P17505 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17505 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1494, Eukaryota |
GeneTreei | ENSGT00940000176501 |
HOGENOMi | CLU_047181_1_0_1 |
InParanoidi | P17505 |
OMAi | MGWTSQA |
Family and domain databases
CDDi | cd01337, MDH_glyoxysomal_mitochondrial, 1 hit |
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR001252, Malate_DH_AS IPR010097, Malate_DH_type1 IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
TIGRFAMsi | TIGR01772, MDH_euk_gproteo, 1 hit |
PROSITEi | View protein in PROSITE PS00068, MDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P17505-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSRVAKRAF SSTVANPYKV TVLGAGGGIG QPLSLLLKLN HKVTDLRLYD
60 70 80 90 100
LKGAKGVATD LSHIPTNSVV KGFTPEEPDG LNNALKDTDM VLIPAGVPRK
110 120 130 140 150
PGMTRDDLFA INASIVRDLA AATAESAPNA AILVISNPVN STVPIVAQVL
160 170 180 190 200
KNKGVYNPKK LFGVTTLDSI RAARFISEVE NTDPTQERVN VIGGHSGITI
210 220 230 240 250
IPLISQTNHK LMSDDKRHEL IHRIQFGGDE VVKAKNGAGS ATLSMAHAGA
260 270 280 290 300
KFANAVLSGF KGERDVIEPS FVDSPLFKSE GIEFFASPVT LGPDGIEKIH
310 320 330
PIGELSSEEE EMLQKCKETL KKNIEKGVNF VASK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | S → G AA sequence (PubMed:3552052).Curated | 1 | |
Sequence conflicti | 306 | S → F in AAS56240 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02841 Genomic DNA Translation: AAA34759.1 Z28085 Genomic DNA Translation: CAA81923.1 AY557914 Genomic DNA Translation: AAS56240.1 BK006944 Genomic DNA Translation: DAA09073.1 |
PIRi | A31945, DEBYMM |
RefSeqi | NP_012838.1, NM_001179651.1 |
Genome annotation databases
EnsemblFungii | YKL085W_mRNA; YKL085W; YKL085W |
GeneIDi | 853777 |
KEGGi | sce:YKL085W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02841 Genomic DNA Translation: AAA34759.1 Z28085 Genomic DNA Translation: CAA81923.1 AY557914 Genomic DNA Translation: AAS56240.1 BK006944 Genomic DNA Translation: DAA09073.1 |
PIRi | A31945, DEBYMM |
RefSeqi | NP_012838.1, NM_001179651.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HR9 | X-ray | 3.01 | O/P/Q/R | 2-9 | [»] | |
AlphaFoldDBi | P17505 | |||||
SMRi | P17505 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34048, 134 interactors |
DIPi | DIP-5734N |
IntActi | P17505, 31 interactors |
STRINGi | 4932.YKL085W |
PTM databases
iPTMneti | P17505 |
2D gel databases
UCD-2DPAGEi | P17505 |
Proteomic databases
MaxQBi | P17505 |
PaxDbi | P17505 |
PRIDEi | P17505 |
TopDownProteomicsi | P17505 |
Genome annotation databases
EnsemblFungii | YKL085W_mRNA; YKL085W; YKL085W |
GeneIDi | 853777 |
KEGGi | sce:YKL085W |
Organism-specific databases
SGDi | S000001568, MDH1 |
VEuPathDBi | FungiDB:YKL085W |
Phylogenomic databases
eggNOGi | KOG1494, Eukaryota |
GeneTreei | ENSGT00940000176501 |
HOGENOMi | CLU_047181_1_0_1 |
InParanoidi | P17505 |
OMAi | MGWTSQA |
Enzyme and pathway databases
Reactomei | R-SCE-70263, Gluconeogenesis R-SCE-71403, Citric acid cycle (TCA cycle) |
Miscellaneous databases
EvolutionaryTracei | P17505 |
PROi | PR:P17505 |
RNActi | P17505, protein |
Family and domain databases
CDDi | cd01337, MDH_glyoxysomal_mitochondrial, 1 hit |
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR001252, Malate_DH_AS IPR010097, Malate_DH_type1 IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
TIGRFAMsi | TIGR01772, MDH_euk_gproteo, 1 hit |
PROSITEi | View protein in PROSITE PS00068, MDH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MDHM_YEAST | |
Accessioni | P17505Primary (citable) accession number: P17505 Secondary accession number(s): D6VXK3, E9P8U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 201 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families