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Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) (RV-A)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inner capsid protein that self-assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle (PubMed:20122940). It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core (PubMed:20122940). The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus (PubMed:20122940). Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels formed by VP2 N-termini (PubMed:20122940). VP2 is required for the replicase activity of VP1 polymerase (By similarity). Probably recruits a copy of a VP1-VP3 complex, potentially along with a segment of plus-strand RNA, as a decamer of VP2 assembles (PubMed:20122940). May activate the autoinhibited VP1/RNA complex to coordinate packaging and genome replication (By similarity).UniRule annotation1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner capsid protein VP2UniRule annotation
OrganismiRotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) (RV-A)
Taxonomic identifieri10934 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000008657 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation1 Publication
  • Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).UniRule annotation1 Publication

GO - Cellular componenti

  • T=2 icosahedral viral capsid Source: UniProtKB
  • viral inner capsid Source: UniProtKB
  • viral nucleocapsid Source: InterPro

Keywords - Cellular componenti

Capsid protein, Inner capsid protein, T=2 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495321 – 881Inner capsid protein VP2Add BLAST881

Post-translational modificationi

Sumoylated with SUMO1 and SUMO2. Sumoylation of viral proteins seems to have a positive role on viral replication.UniRule annotation

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiP17462

Interactioni

Subunit structurei

Homodecamer; each decamer is made up of two conformers of VP2, called VP2A and VP2B (PubMed:20122940). Interacts with a VP1-VP3 complex (PubMed:20122940). Interacts with the intermediate capsid protein VP6 (PubMed:20122940). Interacts with NSP5 (By similarity). Interacts (via N-terminus) with NSP2 (By similarity).UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei220Interaction with the intermediate capsid protein VP6UniRule annotation1 Publication1
Sitei224Interaction with the intermediate capsid protein VP6UniRule annotation1 Publication1
Sitei228Interaction with the intermediate capsid protein VP6UniRule annotation1 Publication1
Sitei840Interaction with the intermediate capsid protein VP6UniRule annotation1 Publication1
Sitei842Interaction with the intermediate capsid protein VP6UniRule annotation1 Publication1

Structurei

3D structure databases

SMRiP17462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 805-fold hub; involved in the encapsidation of VP1 and VP3UniRule annotation1 PublicationAdd BLAST80
Regioni394 – 414HydrophobicUniRule annotationAdd BLAST21
Regioni422 – 442HydrophobicUniRule annotationAdd BLAST21

Domaini

The N-terminus binds RNA (By similarity). It is necessary for encapsidation of VP1 and VP3 (By similarity). The N-termini of 10 VP2 molecules form a cylindrical hub underneath each 5-fold axis of the inner capsid (PubMed:20122940).UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the rotavirus VP2 family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

OrthoDBiVOG0900001T

Family and domain databases

HAMAPiMF_04123 Rota_VP2, 1 hit
MF_04127 Rota_VP2_A, 1 hit
InterProiView protein in InterPro
IPR007779 Rotavirus_VP2
PfamiView protein in Pfam
PF05087 Rota_VP2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD008866 Rota_VP2, 1 hit

Sequencei

Sequence statusi: Complete.

P17462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRKRGATV EADINNNDRM QEKDDEKQDQ NNRMQLSDKV LSKKEEVVTD
60 70 80 90 100
SQEEIKIRDE VKKSTKEESK QLLEVLKTKE EHQKEIQYEI LQKTIPTFEP
110 120 130 140 150
KESILKKLED IKPEQAKKQT KLFRIFEPRQ LPIYRANGEK ELRNRWYWKL
160 170 180 190 200
KKDTLPDGDY DVREYFLNLY DQVLTEMPDY LLLKDMAVEN KNSRDAGKVV
210 220 230 240 250
DSETASICDA IFQDEETEGA VRRFIAEMRQ RVQADRNVVN YPSILHPIDY
260 270 280 290 300
AFNEYFLQHQ LVEPLNNDII FNYIPERIRN DVNYILNMDR NLPSTARYIR
310 320 330 340 350
PNLLQDRLNL HDNFESLWDT ITTSNYILAR SVVPDLKELV STEAQIQKMS
360 370 380 390 400
QDLQLEALTI QSETQFLTGI NSQAANDCFK TLIAAMLSQR TMSLDFVTTN
410 420 430 440 450
YMSLISGMWL LTVVPNDMFI RESLVACQLA IVNTIIYPAF GMQRMHYRNG
460 470 480 490 500
DPQTPFQIAE QQIRKFSGSG IGWHFVNNNQ FRQVVIDGVL NQVLNDNIRN
510 520 530 540 550
VHVIKQLMQA LMQLSRQQFP TMPVDYKRSI QRGILLLSNR LGQLVDLTRL
560 570 580 590 600
LAYNYETLMA CVTMNMQHVQ TLTTEKLQLT SVTSLCMLIG NATVIPSPQT
610 620 630 640 650
LFHYYNVNVN FHSNYNERIN DAVAIITAAN RLNLYQKKMK AIVEDFLKRL
660 670 680 690 700
HIFDVARVPD DQMYRLRDRL RLLPVEVRRL DIFNLILMNM DQIERASDKI
710 720 730 740 750
AQGVIIAYRD MQLERDEMYG YVNIARNLDG FQQINLEELM RTGDYAQITN
760 770 780 790 800
MLLNNQPVAL VGALPFVTDS SVISLIAKLD ATVFAQIVKL RKVDTLKPIL
810 820 830 840 850
YKINSDSNDF YLVANYDWVP TSTTKVYKQV PQQFDFRNSM HMLTSNLTFT
860 870 880
VYSDLLAFVS ADTVEPINAV AFDNMRIMNE L
Length:881
Mass (Da):102,488
Last modified:August 1, 1990 - v1
Checksum:i7CFCDF653E4CF2CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52589 Genomic RNA Translation: CAA36825.1

Similar proteinsi

Entry informationi

Entry nameiVP2_ROTBU
AccessioniPrimary (citable) accession number: P17462
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 20, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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