UniProtKB - P17446 (BETI_ECOLI)
Protein
HTH-type transcriptional regulator BetI
Gene
betI
Organism
Escherichia coli (strain K12)
Status
Functioni
Repressor involved in the biosynthesis of the osmoprotectant glycine betaine. It represses transcription of the choline transporter BetT and the genes of BetAB involved in the synthesis of glycine betaine.2 Publications
Miscellaneous
BetI is an unusual repressor because it remains bound to DNA during induction.1 Publication
: betaine biosynthesis via choline pathway Pathwayi
This protein regulates the pathway betaine biosynthesis via choline pathway, which is part of Amine and polyamine biosynthesis.View all proteins of this organism that are known to be involved in the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 31 – 50 | H-T-H motifUniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: EcoCyc
- DNA-binding transcription factor activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- glycine betaine biosynthetic process from choline Source: UniProtKB-UniRule
- negative regulation of transcription, DNA-templated Source: EcoCyc
- regulation of transcription, DNA-templated Source: GO_Central
- response to osmotic stress Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00251 |
UniPathwayi | UPA00529 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator BetIUniRule annotation |
Gene namesi | Name:betIUniRule annotation Ordered Locus Names:b0313, JW0305 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
In cells lacking this gene, expression of the bet promoters is stimulated by osmotic stress but not by choline.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070580 | 1 – 195 | HTH-type transcriptional regulator BetIAdd BLAST | 195 |
Proteomic databases
PaxDbi | P17446 |
PRIDEi | P17446 |
Expressioni
Inductioni
Negatively autoregulated.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262808, 6 interactors 849378, 1 interactor |
IntActi | P17446, 10 interactors |
STRINGi | 511145.b0313 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 68 | HTH tetR-typeUniRule annotationAdd BLAST | 61 |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
HOGENOMi | CLU_069356_15_4_6 |
InParanoidi | P17446 |
PhylomeDBi | P17446 |
Family and domain databases
HAMAPi | MF_00768, HTH_type_BetI, 1 hit |
InterProi | View protein in InterPro IPR039538, BetI_C IPR023772, DNA-bd_HTH_TetR-type_CS IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR036271, Tet_transcr_reg_TetR-rel_C_sf IPR017757, Tscrpt_rep_BetI |
Pfami | View protein in Pfam PF13977, TetR_C_6, 1 hit PF00440, TetR_N, 1 hit |
PRINTSi | PR00455, HTHTETR |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF48498, SSF48498, 1 hit |
TIGRFAMsi | TIGR03384, betaine_BetI, 1 hit |
PROSITEi | View protein in PROSITE PS01081, HTH_TETR_1, 1 hit PS50977, HTH_TETR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P17446-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKLGMQSIR RRQLIDATLE AINEVGMHDA TIAQIARRAG VSTGIISHYF
60 70 80 90 100
RDKNGLLEAT MRDITSQLRD AVLNRLHALP QGSAEQRLQA IVGGNFDETQ
110 120 130 140 150
VSSAAMKAWL AFWASSMHQP MLYRLQQVSS RRLLSNLVSE FRRELPREQA
160 170 180 190
QEAGYGLAAL IDGLWLRAAL SGKPLDKTRA NSLTRHFITQ HLPTD
Sequence cautioni
The sequence AAB18039 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52905 Genomic DNA Translation: CAA37091.1 U73857 Genomic DNA Translation: AAB18039.1 Different initiation. U00096 Genomic DNA Translation: AAC73416.1 AP009048 Genomic DNA Translation: BAE76096.1 |
PIRi | S15180, S10899 |
RefSeqi | NP_414847.3, NC_000913.3 WP_001335745.1, NZ_SSZK01000067.1 |
Genome annotation databases
EnsemblBacteriai | AAC73416; AAC73416; b0313 BAE76096; BAE76096; BAE76096 |
GeneIDi | 57730749 944981 |
KEGGi | ecj:JW0305 eco:b0313 |
PATRICi | fig|1411691.4.peg.1964 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52905 Genomic DNA Translation: CAA37091.1 U73857 Genomic DNA Translation: AAB18039.1 Different initiation. U00096 Genomic DNA Translation: AAC73416.1 AP009048 Genomic DNA Translation: BAE76096.1 |
PIRi | S15180, S10899 |
RefSeqi | NP_414847.3, NC_000913.3 WP_001335745.1, NZ_SSZK01000067.1 |
3D structure databases
SMRi | P17446 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262808, 6 interactors 849378, 1 interactor |
IntActi | P17446, 10 interactors |
STRINGi | 511145.b0313 |
Proteomic databases
PaxDbi | P17446 |
PRIDEi | P17446 |
Genome annotation databases
EnsemblBacteriai | AAC73416; AAC73416; b0313 BAE76096; BAE76096; BAE76096 |
GeneIDi | 57730749 944981 |
KEGGi | ecj:JW0305 eco:b0313 |
PATRICi | fig|1411691.4.peg.1964 |
Organism-specific databases
EchoBASEi | EB0109 |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
HOGENOMi | CLU_069356_15_4_6 |
InParanoidi | P17446 |
PhylomeDBi | P17446 |
Enzyme and pathway databases
UniPathwayi | UPA00529 |
BioCyci | EcoCyc:PD00251 |
Miscellaneous databases
PROi | PR:P17446 |
Family and domain databases
HAMAPi | MF_00768, HTH_type_BetI, 1 hit |
InterProi | View protein in InterPro IPR039538, BetI_C IPR023772, DNA-bd_HTH_TetR-type_CS IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR036271, Tet_transcr_reg_TetR-rel_C_sf IPR017757, Tscrpt_rep_BetI |
Pfami | View protein in Pfam PF13977, TetR_C_6, 1 hit PF00440, TetR_N, 1 hit |
PRINTSi | PR00455, HTHTETR |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF48498, SSF48498, 1 hit |
TIGRFAMsi | TIGR03384, betaine_BetI, 1 hit |
PROSITEi | View protein in PROSITE PS01081, HTH_TETR_1, 1 hit PS50977, HTH_TETR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BETI_ECOLI | |
Accessioni | P17446Primary (citable) accession number: P17446 Secondary accession number(s): Q2MCB0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways