The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P17443 (MURG_ECOLI)
Protein
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Gene
murG
Organism
Escherichia coli (strain K12)
Status
Functioni
Cell wall formation (PubMed:1649817).
Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (PubMed:1649817, PubMed:12538870).
Strongly prefers UDP to CDP, GDP, ADP and TDP (PubMed:12538870).
2 PublicationsCatalytic activityi
- di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-α-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-α-D-glucosaminyl-(1→4)]-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H+ + UDPUniRule annotation3 PublicationsEC:2.4.1.227UniRule annotation3 Publications
Kineticsi
kcat is 837 min(-1).1 Publication
- KM=0.053 mM for lipid I1 Publication
- KM=0.053 mM for UDP-N-acetyl-alpha-D-glucosamine1 Publication
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 127 | UDP-N-acetyl-alpha-D-glucosamineUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 163 | UDP-N-acetyl-alpha-D-glucosamineUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 191 | UDP-N-acetyl-alpha-D-glucosamineUniRule annotation | 1 | |
Binding sitei | 244 | UDP-N-acetyl-alpha-D-glucosamine; via amide nitrogen and carbonyl oxygenUniRule annotationCombined sources1 Publication | 1 |
GO - Molecular functioni
- glycosyltransferase activity Source: GO_Central
- UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity Source: UniProtKB-EC
- undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Source: EcoliWiki
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- lipid glycosylation Source: UniProtKB-UniRule
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:NACGLCTRANS-MONOMER |
BRENDAi | 2.4.1.227, 2026 |
SABIO-RKi | P17443 |
UniPathwayi | UPA00219 |
Protein family/group databases
CAZyi | GT28, Glycosyltransferase Family 28 |
Chemistry databases
SwissLipidsi | SLP:000001814 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseUniRule annotation (EC:2.4.1.227UniRule annotation2 Publications)Alternative name(s): Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferaseUniRule annotation |
Gene namesi | Name:murG1 PublicationUniRule annotation Ordered Locus Names:b0090, JW0088 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation3 Publications; Peripheral membrane protein UniRule annotation3 Publications; Cytoplasmic side UniRule annotation1 Publication
Note: Randomly distributed in the cell envelope with a higher intensity at the division site. Mid-cell localization requires divisome components.1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Inactivation of the gene rapidly inhibits peptidoglycan synthesis in exponentially growing cells.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | T → A: 387-fold decrease in kcat. 1 Publication | 1 | |
Mutagenesisi | 18 | H → A: Almost loss of activity. 1 Publication | 1 | |
Mutagenesisi | 127 | N → A: 64-fold decrase in kcat. 1 Publication | 1 | |
Mutagenesisi | 191 | S → A: 40-fold decrease in kcat. 1 Publication | 1 | |
Mutagenesisi | 268 | E → A: 1.6-fold decrease in kcat. 1 Publication | 1 | |
Mutagenesisi | 268 | E → D: 1.4-fold decrease in kcat. 1 Publication | 1 | |
Mutagenesisi | 288 | Q → A: 114-fold decrease in kcat. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3596078 |
DrugBanki | DB02196, Uridine-Diphosphate-N-Acetylgalactosamine |
DrugCentrali | P17443 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000109170 | 2 – 355 | UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseAdd BLAST | 354 |
Proteomic databases
jPOSTi | P17443 |
PaxDbi | P17443 |
PRIDEi | P17443 |
Interactioni
Subunit structurei
Binary interactionsi
P17443
With | #Exp. | IntAct |
---|---|---|
yfiF [P0AGJ5] | 3 | EBI-554881,EBI-547368 |
Protein-protein interaction databases
BioGRIDi | 4261859, 572 interactors 850678, 1 interactor |
DIPi | DIP-10282N |
IntActi | P17443, 27 interactors |
STRINGi | 511145.b0090 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P17443 |
SMRi | P17443 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17443 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 17 | UDP-N-acetyl-alpha-D-glucosamine bindingUniRule annotationCombined sources1 Publication | 3 | |
Regioni | 263 – 268 | UDP-N-acetyl-alpha-D-glucosamine bindingUniRule annotationCombined sources1 Publication | 6 | |
Regioni | 287 – 288 | UDP-N-acetyl-alpha-D-glucosamine bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0707, Bacteria |
HOGENOMi | CLU_037404_2_0_6 |
InParanoidi | P17443 |
OMAi | AADMMLC |
PhylomeDBi | P17443 |
Family and domain databases
HAMAPi | MF_00033, MurG, 1 hit |
InterProi | View protein in InterPro IPR006009, GlcNAc_MurG IPR007235, Glyco_trans_28_C IPR004276, GlycoTrans_28_N |
Pfami | View protein in Pfam PF04101, Glyco_tran_28_C, 1 hit PF03033, Glyco_transf_28, 1 hit |
TIGRFAMsi | TIGR01133, murG, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P17443-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGQGKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEADLV
60 70 80 90 100
PKHGIEIDFI RISGLRGKGI KALIAAPLRI FNAWRQARAI MKAYKPDVVL
110 120 130 140 150
GMGGYVSGPG GLAAWSLGIP VVLHEQNGIA GLTNKWLAKI ATKVMQAFPG
160 170 180 190 200
AFPNAEVVGN PVRTDVLALP LPQQRLAGRE GPVRVLVVGG SQGARILNQT
210 220 230 240 250
MPQVAAKLGD SVTIWHQSGK GSQQSVEQAY AEAGQPQHKV TEFIDDMAAA
260 270 280 290 300
YAWADVVVCR SGALTVSEIA AAGLPALFVP FQHKDRQQYW NALPLEKAGA
310 320 330 340 350
AKIIEQPQLS VDAVANTLAG WSRETLLTMA ERARAASIPD ATERVANEVS
RVARA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52644 Genomic DNA Translation: CAA36867.1 X52540 Genomic DNA Translation: CAA36776.1 X55034 Genomic DNA Translation: CAA38867.1 U00096 Genomic DNA Translation: AAC73201.1 AP009048 Genomic DNA Translation: BAB96658.1 |
PIRi | JQ0544, BVECMG |
RefSeqi | NP_414632.1, NC_000913.3 WP_000016560.1, NZ_SSZK01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC73201; AAC73201; b0090 BAB96658; BAB96658; BAB96658 |
GeneIDi | 946321 |
KEGGi | ecj:JW0088 eco:b0090 |
PATRICi | fig|1411691.4.peg.2190 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52644 Genomic DNA Translation: CAA36867.1 X52540 Genomic DNA Translation: CAA36776.1 X55034 Genomic DNA Translation: CAA38867.1 U00096 Genomic DNA Translation: AAC73201.1 AP009048 Genomic DNA Translation: BAB96658.1 |
PIRi | JQ0544, BVECMG |
RefSeqi | NP_414632.1, NC_000913.3 WP_000016560.1, NZ_SSZK01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F0K | X-ray | 1.90 | A/B | 1-355 | [»] | |
1NLM | X-ray | 2.50 | A/B | 2-355 | [»] | |
AlphaFoldDBi | P17443 | |||||
SMRi | P17443 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261859, 572 interactors 850678, 1 interactor |
DIPi | DIP-10282N |
IntActi | P17443, 27 interactors |
STRINGi | 511145.b0090 |
Chemistry databases
ChEMBLi | CHEMBL3596078 |
DrugBanki | DB02196, Uridine-Diphosphate-N-Acetylgalactosamine |
DrugCentrali | P17443 |
SwissLipidsi | SLP:000001814 |
Protein family/group databases
CAZyi | GT28, Glycosyltransferase Family 28 |
Proteomic databases
jPOSTi | P17443 |
PaxDbi | P17443 |
PRIDEi | P17443 |
Genome annotation databases
EnsemblBacteriai | AAC73201; AAC73201; b0090 BAB96658; BAB96658; BAB96658 |
GeneIDi | 946321 |
KEGGi | ecj:JW0088 eco:b0090 |
PATRICi | fig|1411691.4.peg.2190 |
Organism-specific databases
EchoBASEi | EB0618 |
Phylogenomic databases
eggNOGi | COG0707, Bacteria |
HOGENOMi | CLU_037404_2_0_6 |
InParanoidi | P17443 |
OMAi | AADMMLC |
PhylomeDBi | P17443 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:NACGLCTRANS-MONOMER |
BRENDAi | 2.4.1.227, 2026 |
SABIO-RKi | P17443 |
Miscellaneous databases
EvolutionaryTracei | P17443 |
PROi | PR:P17443 |
Family and domain databases
HAMAPi | MF_00033, MurG, 1 hit |
InterProi | View protein in InterPro IPR006009, GlcNAc_MurG IPR007235, Glyco_trans_28_C IPR004276, GlycoTrans_28_N |
Pfami | View protein in Pfam PF04101, Glyco_tran_28_C, 1 hit PF03033, Glyco_transf_28, 1 hit |
TIGRFAMsi | TIGR01133, murG, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MURG_ECOLI | |
Accessioni | P17443Primary (citable) accession number: P17443 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 197 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families