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Protein

Transcription factor PU.1

Gene

Spi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi172 – 255ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, RNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PU.1
Alternative name(s):
31 kDa-transforming protein
SFFV proviral integration 1 protein
Gene namesi
Name:Spi1
Synonyms:Sfpi-1, Sfpi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98282 Spi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Involved in murine acute Friend erythroleukemia. It is a target region for SFFV proviral insertion.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041331 – 272Transcription factor PU.1Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei142PhosphoserineBy similarity1
Modified residuei148PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17433
PeptideAtlasiP17433
PRIDEiP17433

PTM databases

iPTMnetiP17433
PhosphoSitePlusiP17433

Expressioni

Gene expression databases

BgeeiENSMUSG00000002111 Expressed in 169 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_SFPI1
ExpressionAtlasiP17433 baseline and differential
GenevisibleiP17433 MM

Interactioni

Subunit structurei

Binds DNA as a monomer. Interacts with RUNX1 and SPIB (By similarity). Interacts with CEBPD and NONO. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Interacts with CEBPE (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203184, 16 interactors
CORUMiP17433
IntActiP17433, 7 interactors
STRINGi10090.ENSMUSP00000002180

Structurei

Secondary structure

1272
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP17433
SMRiP17433
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17433

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiP17433
KOiK09438
OMAiPRMCLPY
OrthoDBiEOG091G0DTY
PhylomeDBiP17433
TreeFamiTF352494

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P17433-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLQACKMEGF SLTAPPSDDL VTYDSELYQR PMHDYYSFVG SDGESHSDHY
60 70 80 90 100
WDFSAHHVHN NEFENFPENH FTELQSVQPP QLQQLYRHME LEQMHVLDTP
110 120 130 140 150
MVPPHTGLSH QVSYMPRMCF PYQTLSPAHQ QSSDEEEGER QSPPLEVSDG
160 170 180 190 200
EADGLEPGPG LLHGETGSKK KIRLYQFLLD LLRSGDMKDS IWWVDKDKGT
210 220 230 240 250
FQFSSKHKEA LAHRWGIQKG NRKKMTYQKM ARALRNYGKT GEVKKVKKKL
260 270
TYQFSGEVLG RGGLAERRLP PH
Length:272
Mass (Da):31,349
Last modified:February 1, 2005 - v2
Checksum:i765E9CC2F374EB6E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QAI9E9QAI9_MOUSE
Transcription factor PU.1
Spi1 Sfpi1
77Annotation score:
E9Q6W1E9Q6W1_MOUSE
Transcription factor PU.1
Spi1 Sfpi1
143Annotation score:

Sequence cautioni

The sequence AAB59699 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA35502 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17463 mRNA Translation: CAA35502.1 Different initiation.
L03215 mRNA Translation: AAB59699.1 Different initiation.
M32370 mRNA Translation: AAA40024.1
M38252 mRNA Translation: AAA40110.1
BC003815 mRNA Translation: AAH03815.1
CCDSiCCDS16425.1
PIRiA34693
RefSeqiNP_035485.1, NM_011355.2
UniGeneiMm.1302

Genome annotation databases

EnsembliENSMUST00000002180; ENSMUSP00000002180; ENSMUSG00000002111
GeneIDi20375
KEGGimmu:20375
UCSCiuc008kuj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17463 mRNA Translation: CAA35502.1 Different initiation.
L03215 mRNA Translation: AAB59699.1 Different initiation.
M32370 mRNA Translation: AAA40024.1
M38252 mRNA Translation: AAA40110.1
BC003815 mRNA Translation: AAH03815.1
CCDSiCCDS16425.1
PIRiA34693
RefSeqiNP_035485.1, NM_011355.2
UniGeneiMm.1302

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PUEX-ray2.10E/F171-259[»]
5W3GNMR-A167-272[»]
ProteinModelPortaliP17433
SMRiP17433
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203184, 16 interactors
CORUMiP17433
IntActiP17433, 7 interactors
STRINGi10090.ENSMUSP00000002180

PTM databases

iPTMnetiP17433
PhosphoSitePlusiP17433

Proteomic databases

PaxDbiP17433
PeptideAtlasiP17433
PRIDEiP17433

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002180; ENSMUSP00000002180; ENSMUSG00000002111
GeneIDi20375
KEGGimmu:20375
UCSCiuc008kuj.1 mouse

Organism-specific databases

CTDi6688
MGIiMGI:98282 Spi1

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiP17433
KOiK09438
OMAiPRMCLPY
OrthoDBiEOG091G0DTY
PhylomeDBiP17433
TreeFamiTF352494

Miscellaneous databases

EvolutionaryTraceiP17433
PROiPR:P17433
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002111 Expressed in 169 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_SFPI1
ExpressionAtlasiP17433 baseline and differential
GenevisibleiP17433 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPI1_MOUSE
AccessioniPrimary (citable) accession number: P17433
Secondary accession number(s): Q99L57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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