UniProtKB - P17427 (AP2A2_MOUSE)
AP-2 complex subunit alpha-2
Ap2a2
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 53 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 |
GO - Molecular functioni
- cargo adaptor activity Source: GO_Central
- clathrin adaptor activity Source: GO_Central
- disordered domain specific binding Source: CAFA
- kinase binding Source: UniProtKB
- phosphatidylinositol binding Source: MGI
- protein-containing complex binding Source: MGI
- protein domain specific binding Source: CAFA
- protein kinase binding Source: ParkinsonsUK-UCL
GO - Biological processi
- clathrin-dependent endocytosis Source: UniProtKB
- intracellular protein transport Source: MGI
- receptor-mediated endocytosis Source: GO_Central
- regulation of hematopoietic stem cell differentiation Source: BHF-UCL
- vesicle-mediated transport Source: MGI
Keywordsi
Biological process | Endocytosis, Protein transport, Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-177504, Retrograde neurotrophin signalling R-MMU-2132295, MHC class II antigen presentation R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-437239, Recycling pathway of L1 R-MMU-5099900, WNT5A-dependent internalization of FZD4 R-MMU-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-MMU-6798695, Neutrophil degranulation R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis R-MMU-8866427, VLDLR internalisation and degradation R-MMU-8964038, LDL clearance |
Names & Taxonomyi
Protein namesi | Recommended name: AP-2 complex subunit alpha-2Alternative name(s): 100 kDa coated vesicle protein C Adaptor protein complex AP-2 subunit alpha-2 Adaptor-related protein complex 2 subunit alpha-2 Alpha-adaptin C Alpha2-adaptin Clathrin assembly protein complex 2 alpha-C large chain Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit |
Gene namesi | Name:Ap2a2 Synonyms:Adtab |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101920, Ap2a2 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Peripheral membrane protein Curated; Cytoplasmic side Curated
Other locations
- coated pit 1 Publication; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.1 Publication
Plasma Membrane
- AP-2 adaptor complex Source: UniProtKB
Other locations
- cytoplasmic vesicle Source: BHF-UCL
- membrane coat Source: MGI
- secretory granule Source: MGI
- synaptic vesicle Source: MGI
Keywords - Cellular componenti
Cell membrane, Coated pit, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | R → E: Reduces interaction with CD4 endocytosis signal motif; when associated with AP2S1 S-15. 1 Publication | 1 | |
Mutagenesisi | 31 | K → Q: Reduces phosphatidylinositol binding. 1 Publication | 1 | |
Mutagenesisi | 32 | R → Q: Reduces phosphatidylinositol binding. 1 Publication | 1 | |
Mutagenesisi | 35 | K → Q: Reduces phosphatidylinositol binding. 1 Publication | 1 | |
Mutagenesisi | 45 | K → Q: Reduces phosphatidylinositol binding. 1 Publication | 1 | |
Mutagenesisi | 55 | K → Q: Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-56 and Q-57. 1 Publication | 1 | |
Mutagenesisi | 56 | K → E: Strongly reduces phosphatidylinositol binding. 1 Publication | 1 | |
Mutagenesisi | 56 | K → Q: Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-55 and Q-57. 1 Publication | 1 | |
Mutagenesisi | 57 | K → Q: Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-55 and Q-56. 1 Publication | 1 | |
Mutagenesisi | 61 | K → Q: Reduces phosphatidylinositol binding. 1 Publication | 1 | |
Mutagenesisi | 727 | K → A: No effect on DENND1A-,DENND1B- nor DENND1C-binding. | 1 | |
Mutagenesisi | 782 | Q → A: Reduces DENND1A- and DENND1C-binding. 1 Publication | 1 | |
Mutagenesisi | 837 | F → A: Reduces SNAP91, AMPH and BIN1 binding. Abolishes AMPH and SNAP91 binding; when associated with A-916. Abolishes EPN1 and EPS15 binding; when associated with A-905. 2 Publications | 1 | |
Mutagenesisi | 840 | W → A: Abolishes AMPH, BIN1, DGKD, EPS15, EPN1, auxilin and SNAP91 binding. 2 Publications | 1 | |
Mutagenesisi | 849 | E → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 905 | R → A: Strongly reduces AMPH, SNAP91, auxilin and BIN1 binding. Abolishes EPN1 and EPS15 binding; when associated with A-837. 2 Publications | 1 | |
Mutagenesisi | 907 | E → A: Strongly reduces AMPH, SNAP91 and BIN1 binding. Slightly reduces EPS15 and auxilin binding. 1 Publication | 1 | |
Mutagenesisi | 916 | R → A: Strongly reduces AMPH and SNAP91 binding. Abolishes DENND1B-binding; no effect on DENND1A-, nor DENND1C-binding. Abolishes AMPH and SNAP91 binding; when associated with A-837. 2 Publications | 1 | |
Mutagenesisi | 920 | R → A: Abolishes AMPH and BIN1 binding. Reduces EPS15, SNAP91 and auxilin binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193733 | 1 – 938 | AP-2 complex subunit alpha-2Add BLAST | 938 |
Proteomic databases
EPDi | P17427 |
jPOSTi | P17427 |
MaxQBi | P17427 |
PaxDbi | P17427 |
PeptideAtlasi | P17427 |
PRIDEi | P17427 |
ProteomicsDBi | 296326 |
PTM databases
iPTMneti | P17427 |
PhosphoSitePlusi | P17427 |
SwissPalmi | P17427 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000002957, Expressed in embryo and 311 other tissues |
Genevisiblei | P17427, MM |
Interactioni
Subunit structurei
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1) (PubMed:19140243). Binds EPN1, EPS15, AMPH, SNAP91 and BIN1 (PubMed:10380931, PubMed:10430869, PubMed:12057195).
Interacts with clathrin (PubMed:10459011).
Interacts with HIP1 (By similarity).
Interacts with DGKD (PubMed:17880279).
Interacts with DENND1A, DENND1B and DENND1C (PubMed:20154091).
Interacts with FCHO1 and DAB2 (PubMed:11247302, PubMed:22484487).
Interacts with ATAT1; this interaction is required for efficient alpha-tubulin acetylation by ATAT1 (PubMed:24097348).
Interacts with KIAA1107 (PubMed:29262337). Together with AP2B1 and AP2M1, it interacts with ADAM10; this interaction facilitates ADAM10 endocytosis from the plasma membrane during long-term potentiation in hippocampal neurons (PubMed:23676497).
Interacts with CLN3 (via dileucine motif) (By similarity).
Interacts with ABCB11; this interaction regulates cell membrane expresion of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation (By similarity).
By similarity12 PublicationsGO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- disordered domain specific binding Source: CAFA
- kinase binding Source: UniProtKB
- protein domain specific binding Source: CAFA
- protein kinase binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 198130, 40 interactors |
ComplexPortali | CPX-5153, AP-2 Adaptor complex, alpha2 variant |
CORUMi | P17427 |
DIPi | DIP-32160N |
IntActi | P17427, 24 interactors |
MINTi | P17427 |
STRINGi | 10090.ENSMUSP00000003038 |
Miscellaneous databases
RNActi | P17427, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P17427 |
SMRi | P17427 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17427 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 5 – 80 | Lipid-bindingAdd BLAST | 76 | |
Regioni | 11 – 12 | Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity | 2 | |
Regioni | 57 – 61 | Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1077, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_1_0_1 |
InParanoidi | P17427 |
OMAi | TKTTQVG |
OrthoDBi | 751651at2759 |
TreeFami | TF300308 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 3.30.310.10, 1 hit |
InterProi | View protein in InterPro IPR017104, AP2_complex_asu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR003164, Clathrin_a-adaptin_app_sub_C IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR009028, Coatomer/calthrin_app_sub_C IPR012295, TBP_dom_sf |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02296, Alpha_adaptin_C, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037091, AP2_complex_alpha, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit SSF55711, SSF55711, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNRYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMGL ALHCIANVGS REMAEAFAGE
160 170 180 190 200
IPKILVAGDT MDSVKQSAAL CLLRLYRTSP DLVPMGDWTS RVVHLLNDQH
210 220 230 240 250
LGVVTAATSL ITTLAQKNPE EFKTSVSLAV SRLSRIVTSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PDPAVRGRLT ECLETILNKA QEPPKSKKVQ
310 320 330 340 350
HSNAKNAVLF EAISLIIHHD SEPNLLVRAC NQLGQFLQHR ETNLRYLALE
360 370 380 390 400
SMCTLASSEF SHEAVKTHIE TVINALKTER DVSVRQRAVD LLYAMCDRSN
410 420 430 440 450
AQQIVAEMLS YLETADYSIR EEIVLKVAIL AEKYAVDYTW YVDTILNLIR
460 470 480 490 500
IAGDYVSEEV WYRVIQIVIN RDDVQGYAAK TVFEALQAPA CHENLVKVGG
510 520 530 540 550
YILGEFGNLI AGDPRSSPLI QFNLLHSKFH LCSVPTRALL LSTYIKFVNL
560 570 580 590 600
FPEVKATIQD VLRSDSQLKN ADVELQQRAV EYLRLSTVAS TDILATVLEE
610 620 630 640 650
MPPFPERESS ILAKLKKKKG PSTVTDLEET KRERSIDVNG GPEPVPASTS
660 670 680 690 700
AASTPSPSAD LLGLGAVPPA PTGPPPSSGG GLLVDVFSDS ASAVAPLAPG
710 720 730 740 750
SEDNFARFVC KNNGVLFENQ LLQIGLKSEF RQNLGRMFIF YGNKTSTQFL
760 770 780 790 800
NFTPTLICAD DLQTNLNLQT KPVDPTVDGG AQVQQVVNIE CISDFTEAPV
810 820 830 840 850
LNIQFRYGGT FQNVSVKLPI TLNKFFQPTE MASQDFFQRW KQLSNPQQEV
860 870 880 890 900
QNIFKAKHPM DTEITKAKII GFGSALLEEV DPNPANFVGA GIIHTKTTQI
910 920 930
GCLLRLEPNL QAQMYRLTLR TSKDTVSQRL CELLSEQF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YUA7 | A0A0J9YUA7_MOUSE | AP-2 complex subunit alpha-2 | Ap2a2 | 131 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 858 – 859 | HP → LE in AAH10597 (PubMed:15489334).Curated | 2 | |
Sequence conflicti | 889 – 890 | GA → VL in CAA33097 (PubMed:2564002).Curated | 2 | |
Sequence conflicti | 889 – 890 | GA → VL in AAB62703 (PubMed:9618202).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14972 mRNA Translation: CAA33097.1 AK088500 mRNA Translation: BAC40392.1 AC158224 Genomic DNA No translation available. AC102524 Genomic DNA No translation available. BC010597 mRNA Translation: AAH10597.1 AF006990 mRNA Translation: AAB62703.1 |
CCDSi | CCDS40188.1 |
PIRi | B30111 S12471 |
RefSeqi | NP_031485.3, NM_007459.3 |
Genome annotation databases
Ensembli | ENSMUST00000003038; ENSMUSP00000003038; ENSMUSG00000002957 |
GeneIDi | 11772 |
KEGGi | mmu:11772 |
UCSCi | uc009kls.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14972 mRNA Translation: CAA33097.1 AK088500 mRNA Translation: BAC40392.1 AC158224 Genomic DNA No translation available. AC102524 Genomic DNA No translation available. BC010597 mRNA Translation: AAH10597.1 AF006990 mRNA Translation: AAB62703.1 |
CCDSi | CCDS40188.1 |
PIRi | B30111 S12471 |
RefSeqi | NP_031485.3, NM_007459.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B9K | X-ray | 1.90 | A | 701-938 | [»] | |
1KY6 | X-ray | 2.00 | A | 701-938 | [»] | |
1KY7 | X-ray | 2.15 | A | 701-938 | [»] | |
1KYD | X-ray | 2.00 | A | 701-938 | [»] | |
1KYF | X-ray | 1.22 | A | 701-938 | [»] | |
1KYU | X-ray | 1.80 | A | 701-938 | [»] | |
1QTP | X-ray | 1.60 | A | 701-938 | [»] | |
1QTS | X-ray | 1.40 | A | 701-938 | [»] | |
1W80 | X-ray | 1.90 | A | 695-938 | [»] | |
2JKR | X-ray | 2.98 | A/L | 1-620 | [»] | |
2JKT | X-ray | 3.40 | A/L | 1-620 | [»] | |
2VJ0 | X-ray | 1.60 | A | 695-938 | [»] | |
3HS8 | X-ray | 1.90 | A | 702-938 | [»] | |
6OWO | electron microscopy | 3.20 | A | 1-620 | [»] | |
6OXL | electron microscopy | 3.50 | A | 1-620 | [»] | |
BMRBi | P17427 | |||||
SMRi | P17427 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198130, 40 interactors |
ComplexPortali | CPX-5153, AP-2 Adaptor complex, alpha2 variant |
CORUMi | P17427 |
DIPi | DIP-32160N |
IntActi | P17427, 24 interactors |
MINTi | P17427 |
STRINGi | 10090.ENSMUSP00000003038 |
PTM databases
iPTMneti | P17427 |
PhosphoSitePlusi | P17427 |
SwissPalmi | P17427 |
Proteomic databases
EPDi | P17427 |
jPOSTi | P17427 |
MaxQBi | P17427 |
PaxDbi | P17427 |
PeptideAtlasi | P17427 |
PRIDEi | P17427 |
ProteomicsDBi | 296326 |
Protocols and materials databases
Antibodypediai | 4304, 213 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000003038; ENSMUSP00000003038; ENSMUSG00000002957 |
GeneIDi | 11772 |
KEGGi | mmu:11772 |
UCSCi | uc009kls.2, mouse |
Organism-specific databases
CTDi | 161 |
MGIi | MGI:101920, Ap2a2 |
Phylogenomic databases
eggNOGi | KOG1077, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_1_0_1 |
InParanoidi | P17427 |
OMAi | TKTTQVG |
OrthoDBi | 751651at2759 |
TreeFami | TF300308 |
Enzyme and pathway databases
Reactomei | R-MMU-177504, Retrograde neurotrophin signalling R-MMU-2132295, MHC class II antigen presentation R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-437239, Recycling pathway of L1 R-MMU-5099900, WNT5A-dependent internalization of FZD4 R-MMU-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-MMU-6798695, Neutrophil degranulation R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis R-MMU-8866427, VLDLR internalisation and degradation R-MMU-8964038, LDL clearance |
Miscellaneous databases
BioGRID-ORCSi | 11772, 1 hit in 52 CRISPR screens |
ChiTaRSi | Ap2a2, mouse |
EvolutionaryTracei | P17427 |
PROi | PR:P17427 |
RNActi | P17427, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002957, Expressed in embryo and 311 other tissues |
Genevisiblei | P17427, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 3.30.310.10, 1 hit |
InterProi | View protein in InterPro IPR017104, AP2_complex_asu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR003164, Clathrin_a-adaptin_app_sub_C IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR009028, Coatomer/calthrin_app_sub_C IPR012295, TBP_dom_sf |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02296, Alpha_adaptin_C, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037091, AP2_complex_alpha, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit SSF55711, SSF55711, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP2A2_MOUSE | |
Accessioni | P17427Primary (citable) accession number: P17427 Secondary accession number(s): Q8C2J5, Q921V0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 205 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families