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UniProtKB - P17427 (AP2A2_MOUSE)

Entry version 205 (07 Apr 2021)
Sequence version 2 (27 Jul 2011)
Previous versions | rss
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Protein

AP-2 complex subunit alpha-2

Gene

Ap2a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei43Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei53Phosphatidylinositol 3,4,5-trisphosphateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processEndocytosis, Protein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-177504, Retrograde neurotrophin signalling
R-MMU-2132295, MHC class II antigen presentation
R-MMU-416993, Trafficking of GluR2-containing AMPA receptors
R-MMU-437239, Recycling pathway of L1
R-MMU-5099900, WNT5A-dependent internalization of FZD4
R-MMU-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-MMU-6798695, Neutrophil degranulation
R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828, Clathrin-mediated endocytosis
R-MMU-8866427, VLDLR internalisation and degradation
R-MMU-8964038, LDL clearance

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
AP-2 complex subunit alpha-2
Alternative name(s):
100 kDa coated vesicle protein C
Adaptor protein complex AP-2 subunit alpha-2
Adaptor-related protein complex 2 subunit alpha-2
Alpha-adaptin C
Alpha2-adaptin
Clathrin assembly protein complex 2 alpha-C large chain
Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Ap2a2
Synonyms:Adtab
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:101920, Ap2a2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi21R → E: Reduces interaction with CD4 endocytosis signal motif; when associated with AP2S1 S-15. 1 Publication1
Mutagenesisi31K → Q: Reduces phosphatidylinositol binding. 1 Publication1
Mutagenesisi32R → Q: Reduces phosphatidylinositol binding. 1 Publication1
Mutagenesisi35K → Q: Reduces phosphatidylinositol binding. 1 Publication1
Mutagenesisi45K → Q: Reduces phosphatidylinositol binding. 1 Publication1
Mutagenesisi55K → Q: Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-56 and Q-57. 1 Publication1
Mutagenesisi56K → E: Strongly reduces phosphatidylinositol binding. 1 Publication1
Mutagenesisi56K → Q: Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-55 and Q-57. 1 Publication1
Mutagenesisi57K → Q: Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-55 and Q-56. 1 Publication1
Mutagenesisi61K → Q: Reduces phosphatidylinositol binding. 1 Publication1
Mutagenesisi727K → A: No effect on DENND1A-,DENND1B- nor DENND1C-binding. 1
Mutagenesisi782Q → A: Reduces DENND1A- and DENND1C-binding. 1 Publication1
Mutagenesisi837F → A: Reduces SNAP91, AMPH and BIN1 binding. Abolishes AMPH and SNAP91 binding; when associated with A-916. Abolishes EPN1 and EPS15 binding; when associated with A-905. 2 Publications1
Mutagenesisi840W → A: Abolishes AMPH, BIN1, DGKD, EPS15, EPN1, auxilin and SNAP91 binding. 2 Publications1
Mutagenesisi849E → A: No effect. 1 Publication1
Mutagenesisi905R → A: Strongly reduces AMPH, SNAP91, auxilin and BIN1 binding. Abolishes EPN1 and EPS15 binding; when associated with A-837. 2 Publications1
Mutagenesisi907E → A: Strongly reduces AMPH, SNAP91 and BIN1 binding. Slightly reduces EPS15 and auxilin binding. 1 Publication1
Mutagenesisi916R → A: Strongly reduces AMPH and SNAP91 binding. Abolishes DENND1B-binding; no effect on DENND1A-, nor DENND1C-binding. Abolishes AMPH and SNAP91 binding; when associated with A-837. 2 Publications1
Mutagenesisi920R → A: Abolishes AMPH and BIN1 binding. Reduces EPS15, SNAP91 and auxilin binding. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001937331 – 938AP-2 complex subunit alpha-2Add BLAST938

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P17427

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P17427

MaxQB - The MaxQuant DataBase

More...MaxQBi		P17427

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P17427

PeptideAtlas

More...PeptideAtlasi		P17427

PRoteomics IDEntifications database

More...PRIDEi		P17427

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		296326

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P17427

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P17427

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P17427

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in the brain (at protein level).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000002957, Expressed in embryo and 311 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P17427, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1) (PubMed:19140243). Binds EPN1, EPS15, AMPH, SNAP91 and BIN1 (PubMed:10380931, PubMed:10430869, PubMed:12057195).

Interacts with clathrin (PubMed:10459011).

Interacts with HIP1 (By similarity).

Interacts with DGKD (PubMed:17880279).

Interacts with DENND1A, DENND1B and DENND1C (PubMed:20154091).

Interacts with FCHO1 and DAB2 (PubMed:11247302, PubMed:22484487).

Interacts with ATAT1; this interaction is required for efficient alpha-tubulin acetylation by ATAT1 (PubMed:24097348).

Interacts with KIAA1107 (PubMed:29262337). Together with AP2B1 and AP2M1, it interacts with ADAM10; this interaction facilitates ADAM10 endocytosis from the plasma membrane during long-term potentiation in hippocampal neurons (PubMed:23676497).

Interacts with CLN3 (via dileucine motif) (By similarity).

Interacts with ABCB11; this interaction regulates cell membrane expresion of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation (By similarity).

By similarity12 Publications

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		198130, 40 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-5153, AP-2 Adaptor complex, alpha2 variant

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		P17427

Database of interacting proteins

More...DIPi		DIP-32160N

Protein interaction database and analysis system

More...IntActi		P17427, 24 interactors

Molecular INTeraction database

More...MINTi		P17427

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000003038

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P17427, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1938
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P17427

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P17427

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P17427

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni5 – 80Lipid-bindingAdd BLAST76
Regioni11 – 12Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity2
Regioni57 – 61Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity5

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1077, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00950000182838

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_003824_1_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P17427

Identification of Orthologs from Complete Genome Data

More...OMAi		TKTTQVG

Database of Orthologous Groups

More...OrthoDBi		751651at2759

TreeFam database of animal gene trees

More...TreeFami		TF300308

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.25.10.10, 1 hit
3.30.310.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR017104, AP2_complex_asu
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR002553, Clathrin/coatomer_adapt-like_N
IPR003164, Clathrin_a-adaptin_app_sub_C
IPR008152, Clathrin_a/b/g-adaptin_app_Ig
IPR013041, Clathrin_app_Ig-like_sf
IPR009028, Coatomer/calthrin_app_sub_C
IPR012295, TBP_dom_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01602, Adaptin_N, 1 hit
PF02296, Alpha_adaptin_C, 1 hit
PF02883, Alpha_adaptinC2, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF037091, AP2_complex_alpha, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00809, Alpha_adaptinC2, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48371, SSF48371, 1 hit
SSF49348, SSF49348, 1 hit
SSF55711, SSF55711, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P17427-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL 
        60         70         80         90        100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNRYTE KQIGYLFISV 
       110        120        130        140        150
LVNSNSELIR LINNAIKNDL ASRNPTFMGL ALHCIANVGS REMAEAFAGE 
       160        170        180        190        200
IPKILVAGDT MDSVKQSAAL CLLRLYRTSP DLVPMGDWTS RVVHLLNDQH 
       210        220        230        240        250
LGVVTAATSL ITTLAQKNPE EFKTSVSLAV SRLSRIVTSA STDLQDYTYY 
       260        270        280        290        300
FVPAPWLSVK LLRLLQCYPP PDPAVRGRLT ECLETILNKA QEPPKSKKVQ 
       310        320        330        340        350
HSNAKNAVLF EAISLIIHHD SEPNLLVRAC NQLGQFLQHR ETNLRYLALE 
       360        370        380        390        400
SMCTLASSEF SHEAVKTHIE TVINALKTER DVSVRQRAVD LLYAMCDRSN 
       410        420        430        440        450
AQQIVAEMLS YLETADYSIR EEIVLKVAIL AEKYAVDYTW YVDTILNLIR 
       460        470        480        490        500
IAGDYVSEEV WYRVIQIVIN RDDVQGYAAK TVFEALQAPA CHENLVKVGG 
       510        520        530        540        550
YILGEFGNLI AGDPRSSPLI QFNLLHSKFH LCSVPTRALL LSTYIKFVNL 
       560        570        580        590        600
FPEVKATIQD VLRSDSQLKN ADVELQQRAV EYLRLSTVAS TDILATVLEE 
       610        620        630        640        650
MPPFPERESS ILAKLKKKKG PSTVTDLEET KRERSIDVNG GPEPVPASTS 
       660        670        680        690        700
AASTPSPSAD LLGLGAVPPA PTGPPPSSGG GLLVDVFSDS ASAVAPLAPG 
       710        720        730        740        750
SEDNFARFVC KNNGVLFENQ LLQIGLKSEF RQNLGRMFIF YGNKTSTQFL 
       760        770        780        790        800
NFTPTLICAD DLQTNLNLQT KPVDPTVDGG AQVQQVVNIE CISDFTEAPV 
       810        820        830        840        850
LNIQFRYGGT FQNVSVKLPI TLNKFFQPTE MASQDFFQRW KQLSNPQQEV 
       860        870        880        890        900
QNIFKAKHPM DTEITKAKII GFGSALLEEV DPNPANFVGA GIIHTKTTQI 
       910        920        930 
GCLLRLEPNL QAQMYRLTLR TSKDTVSQRL CELLSEQF
Length:938
Mass (Da):104,017
Last modified:July 27, 2011 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i183FE8DFE199DBCA
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YUA7A0A0J9YUA7_MOUSE
AP-2 complex subunit alpha-2
Ap2a2
131Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti858 – 859HP → LE in AAH10597 (PubMed:15489334).Curated2
Sequence conflicti889 – 890GA → VL in CAA33097 (PubMed:2564002).Curated2
Sequence conflicti889 – 890GA → VL in AAB62703 (PubMed:9618202).Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X14972 mRNA Translation: CAA33097.1
AK088500 mRNA Translation: BAC40392.1
AC158224 Genomic DNA No translation available.
AC102524 Genomic DNA No translation available.
BC010597 mRNA Translation: AAH10597.1
AF006990 mRNA Translation: AAB62703.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS40188.1

Protein sequence database of the Protein Information Resource

More...PIRi		B30111
S12471

NCBI Reference Sequences

More...RefSeqi		NP_031485.3, NM_007459.3

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000003038; ENSMUSP00000003038; ENSMUSG00000002957

Database of genes from NCBI RefSeq genomes

More...GeneIDi		11772

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:11772

UCSC genome browser

More...UCSCi		uc009kls.2, mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14972 mRNA Translation: CAA33097.1
AK088500 mRNA Translation: BAC40392.1
AC158224 Genomic DNA No translation available.
AC102524 Genomic DNA No translation available.
BC010597 mRNA Translation: AAH10597.1
AF006990 mRNA Translation: AAB62703.1
CCDSiCCDS40188.1
PIRiB30111
S12471
RefSeqiNP_031485.3, NM_007459.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B9KX-ray1.90A701-938[»]
1KY6X-ray2.00A701-938[»]
1KY7X-ray2.15A701-938[»]
1KYDX-ray2.00A701-938[»]
1KYFX-ray1.22A701-938[»]
1KYUX-ray1.80A701-938[»]
1QTPX-ray1.60A701-938[»]
1QTSX-ray1.40A701-938[»]
1W80X-ray1.90A695-938[»]
2JKRX-ray2.98A/L1-620[»]
2JKTX-ray3.40A/L1-620[»]
2VJ0X-ray1.60A695-938[»]
3HS8X-ray1.90A702-938[»]
6OWOelectron microscopy3.20A1-620[»]
6OXLelectron microscopy3.50A1-620[»]
BMRBiP17427
SMRiP17427
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi198130, 40 interactors
ComplexPortaliCPX-5153, AP-2 Adaptor complex, alpha2 variant
CORUMiP17427
DIPiDIP-32160N
IntActiP17427, 24 interactors
MINTiP17427
STRINGi10090.ENSMUSP00000003038

PTM databases

iPTMnetiP17427
PhosphoSitePlusiP17427
SwissPalmiP17427

Proteomic databases

EPDiP17427
jPOSTiP17427
MaxQBiP17427
PaxDbiP17427
PeptideAtlasiP17427
PRIDEiP17427
ProteomicsDBi296326

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		4304, 213 antibodies

Genome annotation databases

EnsembliENSMUST00000003038; ENSMUSP00000003038; ENSMUSG00000002957
GeneIDi11772
KEGGimmu:11772
UCSCiuc009kls.2, mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		161
MGIiMGI:101920, Ap2a2

Phylogenomic databases

eggNOGiKOG1077, Eukaryota
GeneTreeiENSGT00950000182838
HOGENOMiCLU_003824_1_0_1
InParanoidiP17427
OMAiTKTTQVG
OrthoDBi751651at2759
TreeFamiTF300308

Enzyme and pathway databases

ReactomeiR-MMU-177504, Retrograde neurotrophin signalling
R-MMU-2132295, MHC class II antigen presentation
R-MMU-416993, Trafficking of GluR2-containing AMPA receptors
R-MMU-437239, Recycling pathway of L1
R-MMU-5099900, WNT5A-dependent internalization of FZD4
R-MMU-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-MMU-6798695, Neutrophil degranulation
R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828, Clathrin-mediated endocytosis
R-MMU-8866427, VLDLR internalisation and degradation
R-MMU-8964038, LDL clearance

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		11772, 1 hit in 52 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Ap2a2, mouse
EvolutionaryTraceiP17427

Protein Ontology

More...PROi		PR:P17427
RNActiP17427, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000002957, Expressed in embryo and 311 other tissues
GenevisibleiP17427, MM

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.310.10, 1 hit
InterProiView protein in InterPro
IPR017104, AP2_complex_asu
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR002553, Clathrin/coatomer_adapt-like_N
IPR003164, Clathrin_a-adaptin_app_sub_C
IPR008152, Clathrin_a/b/g-adaptin_app_Ig
IPR013041, Clathrin_app_Ig-like_sf
IPR009028, Coatomer/calthrin_app_sub_C
IPR012295, TBP_dom_sf
PfamiView protein in Pfam
PF01602, Adaptin_N, 1 hit
PF02296, Alpha_adaptin_C, 1 hit
PF02883, Alpha_adaptinC2, 1 hit
PIRSFiPIRSF037091, AP2_complex_alpha, 1 hit
SMARTiView protein in SMART
SM00809, Alpha_adaptinC2, 1 hit
SUPFAMiSSF48371, SSF48371, 1 hit
SSF49348, SSF49348, 1 hit
SSF55711, SSF55711, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAP2A2_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P17427
Secondary accession number(s): Q8C2J5, Q921V0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: July 27, 2011
Last modified: April 7, 2021
This is version 205 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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