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Protein

AP-2 complex subunit alpha-1

Gene

Ap2a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-177504 Retrograde neurotrophin signalling
R-MMU-2132295 MHC class II antigen presentation
R-MMU-416993 Trafficking of GluR2-containing AMPA receptors
R-MMU-437239 Recycling pathway of L1
R-MMU-5099900 WNT5A-dependent internalization of FZD4
R-MMU-5140745 WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-8866427 VLDLR internalisation and degradation
R-MMU-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alpha-1
Alternative name(s):
100 kDa coated vesicle protein A
Adaptor protein complex AP-2 subunit alpha-1
Adaptor-related protein complex 2 subunit alpha-1
Alpha-adaptin A
Alpha1-adaptin
Clathrin assembly protein complex 2 alpha-A large chain
Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Gene namesi
Name:Ap2a1
Synonyms:Adtaa, Clapa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:101921 Ap2a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937311 – 977AP-2 complex subunit alpha-1Add BLAST977

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei626PhosphoserineBy similarity1
Modified residuei652PhosphoserineCombined sources1
Modified residuei653PhosphothreonineCombined sources1
Modified residuei655PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17426
PaxDbiP17426
PeptideAtlasiP17426
PRIDEiP17426

PTM databases

iPTMnetiP17426
PhosphoSitePlusiP17426
SwissPalmiP17426

Miscellaneous databases

PMAP-CutDBiP17426

Expressioni

Tissue specificityi

Isoform A is expressed only in neuronal tissue and skeletal muscle. Isoform B is widely expressed.

Gene expression databases

BgeeiENSMUSG00000060279
CleanExiMM_AP2A1
ExpressionAtlasiP17426 baseline and differential
GenevisibleiP17426 MM

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Interacts with HIP1 and RAB11FIP2 (By similarity). Interacts with SLC12A5 (PubMed:18625303). Interacts with clathrin (PubMed:7559550). Interacts with SGIP1 (PubMed:17626015). Interacts with RFTN1 (By similarity). Interacts with KIAA1107 (PubMed:29262337).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Grin2bQ010972EBI-775189,EBI-400125

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198129, 14 interactors
CORUMiP17426
IntActiP17426, 20 interactors
MINTiP17426
STRINGi10090.ENSMUSP00000127842

Structurei

3D structure databases

ProteinModelPortaliP17426
SMRiP17426
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1077 Eukaryota
ENOG410XNQE LUCA
GeneTreeiENSGT00550000074757
HOGENOMiHOG000170596
HOVERGENiHBG050518
InParanoidiP17426
KOiK11824
OMAiHNELFNC
OrthoDBiEOG091G01JZ
PhylomeDBiP17426
TreeFamiTF300308

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.310.10, 1 hit
InterProiView protein in InterPro
IPR017104 AP2_complex_asu
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
IPR003164 Clathrin_a-adaptin_app_sub_C
IPR008152 Clathrin_a/b/g-adaptin_app_Ig
IPR013041 Clathrin_app_Ig-like_sf
IPR009028 Coatomer/calthrin_app_sub_C
IPR012295 TBP_dom_sf
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF02296 Alpha_adaptin_C, 1 hit
PF02883 Alpha_adaptinC2, 1 hit
PIRSFiPIRSF037091 AP2_complex_alpha, 1 hit
SMARTiView protein in SMART
SM00809 Alpha_adaptinC2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF49348 SSF49348, 1 hit
SSF55711 SSF55711, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P17426-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD
160 170 180 190 200
IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH
210 220 230 240 250
MGVVTAAVSL ITCLCKKNPD DFKTCISLAV SRLSRIVSSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV
310 320 330 340 350
QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL
360 370 380 390 400
ESMCTLASSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS
410 420 430 440 450
NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI
460 470 480 490 500
RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG
510 520 530 540 550
GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN
560 570 580 590 600
LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE
610 620 630 640 650
EMPPFPERES SILAKLKRKK GPGAASALDD SRRDTSSNDI NGGVEPTPST
660 670 680 690 700
VSTPSPSADL LGLRAAPPPA APPAPVGGNL LVDVFSDGPT AQPSLGPTPE
710 720 730 740 750
EAFLSELEPP APESPMALLA DPAPAADPGP EDIGPPIPEA DELLNKFVCK
760 770 780 790 800
NSGVLFENQL LQIGVKSEFR QNLGRMYLFY GNKTSVQFQN FLPTVVHPGD
810 820 830 840 850
LQTQLAVQTK RVAAQVDGGA QVQQVLNIEC LRDFLTPPLL SVRFRYGGTA
860 870 880 890 900
QSLTLKLPVT INKFFQPTEM AAQDFFQRWK QLSLPLQEAQ KIFKANHPMD
910 920 930 940 950
AEVTKAKLLG FGSALLDNVD PNPENFVGAG IIQTKALQVG CLLRLEPNAQ
960 970
AQMYRLTLRT SKEPVSRHLC ELLAQQF
Length:977
Mass (Da):107,664
Last modified:August 1, 1990 - v1
Checksum:iF4ED87D3F9EF230A
GO
Isoform B (identifier: P17426-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     706-727: Missing.

Show »
Length:955
Mass (Da):105,480
Checksum:iD6EB4672359D66B9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000162706 – 727Missing in isoform B. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14971 mRNA Translation: CAA33096.1
BC031433 mRNA Translation: AAH31433.1
CCDSiCCDS52238.1 [P17426-2]
CCDS52239.1 [P17426-1]
PIRiA30111
RefSeqiNP_001070732.1, NM_001077264.1 [P17426-2]
NP_031484.1, NM_007458.2 [P17426-1]
UniGeneiMm.6877

Genome annotation databases

EnsembliENSMUST00000085399; ENSMUSP00000082519; ENSMUSG00000060279 [P17426-1]
ENSMUST00000107857; ENSMUSP00000103489; ENSMUSG00000060279 [P17426-2]
ENSMUST00000166972; ENSMUSP00000127842; ENSMUSG00000060279 [P17426-1]
ENSMUST00000167930; ENSMUSP00000127497; ENSMUSG00000060279 [P17426-2]
GeneIDi11771
KEGGimmu:11771
UCSCiuc009grv.1 mouse [P17426-1]
uc009grw.1 mouse [P17426-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAP2A1_MOUSE
AccessioniPrimary (citable) accession number: P17426
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 18, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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