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UniProtKB - P17411 (CHBF_ECOLI)
Protein
6-phospho-beta-glucosidase
Gene
chbF
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl-beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose.
2 PublicationsCaution
Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- NAD+1 Publication
- Mn2+1 Publication, Co2+1 Publication, Ni2+1 PublicationNote: Divalent metal ion. Manganese, cobalt and nickel ions enhance activity whereas magnesium, calcium, strontium and zinc ions do not.1 Publication
Kineticsi
- KM=1.30 mM for cellobiose-6P1 Publication
- KM=0.44 mM for salicin-6P1 Publication
- KM=0.35 mM for arbutin-6P1 Publication
- KM=12.50 mM for gentiobiose-6P1 Publication
- KM=2.22 mM for methyl-beta-glucoside-6P1 Publication
- KM=0.44 mM for p-nitrophenyl-beta-D-glucopyranoside-6P1 Publication
- Vmax=1.74 µmol/min/mg enzyme toward cellobiose-6P1 Publication
- Vmax=1.38 µmol/min/mg enzyme toward salicin-6P1 Publication
- Vmax=2.13 µmol/min/mg enzyme toward arbutin-6P1 Publication
- Vmax=1.11 µmol/min/mg enzyme toward gentiobiose-6P1 Publication
- Vmax=0.56 µmol/min/mg enzyme toward methyl-beta-glucoside-6P1 Publication
- Vmax=1.33 µmol/min/mg enzyme toward p-nitrophenyl-beta-D-glucopyranoside-6P1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 96 | SubstrateBy similarity | 1 | |
Sitei | 112 | Increases basicity of active site TyrBy similarity | 1 | |
Binding sitei | 150 | SubstrateBy similarity | 1 | |
Metal bindingi | 172 | ManganeseBy similarity | 1 | |
Metal bindingi | 203 | ManganeseBy similarity | 1 | |
Active sitei | 258 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 5 – 73 | NADBy similarityAdd BLAST | 69 |
GO - Molecular functioni
- 6-phospho-beta-glucosidase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- methyl beta-D-glucoside 6-phosphate glucohydrolase activity Source: UniProtKB-EC
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Cobalt, Manganese, Metal-binding, NAD, Nickel |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10144-MONOMER |
BRENDAi | 3.2.1.86, 2026 |
SABIO-RKi | P17411 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phospho-beta-glucosidase (EC:3.2.1.861 Publication)Alternative name(s): Cellobiose-6-phosphate hydrolase Phospho-chitobiase |
Gene namesi | Name:chbF Synonyms:celF, ydjD Ordered Locus Names:b1734, JW1723 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000169858 | 2 – 450 | 6-phospho-beta-glucosidaseAdd BLAST | 449 |
Proteomic databases
PaxDbi | P17411 |
PRIDEi | P17411 |
Expressioni
Inductioni
By N,N'-diacetylchitobiose.
Interactioni
Subunit structurei
Homotetramer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259132, 399 interactors |
IntActi | P17411, 4 interactors |
STRINGi | 511145.b1734 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 4 family.Curated
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_0_1_6 |
InParanoidi | P17411 |
PhylomeDBi | P17411 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P17411-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQKLKVVTI GGGSSYTPEL LEGFIKRYHE LPVSELWLVD VEGGKPKLDI
60 70 80 90 100
IFDLCQRMID NAGVPMKLYK TLDRREALKD ADFVTTQLRV GQLPARELDE
110 120 130 140 150
RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVEELC PNAWVINFTN
160 170 180 190 200
PAGMVTEAVY RHTGFKRFIG VCNIPIGMKM FIRDVLMLKD SDDLSIDLFG
210 220 230 240 250
LNHMVFIKDV LINGKSRFAE LLDGVASGQL KASSVKNIFD LPFSEGLIRS
260 270 280 290 300
LNLLPCSYLL YYFKQKEMLA IEMGEYYKGG ARAQVVQKVE KQLFELYKNP
310 320 330 340 350
ELKVKPKELE QRGGAYYSDA ACEVINAIYN DKQAEHYVNI PHHGQIDNIP
360 370 380 390 400
ADWAVEMTCK LGRDGATPHP RITHFDDKVM GLIHTIKGFE IAASNAALSG
410 420 430 440 450
EFNDVLLALN LSPLVHSDRD AELLAREMIL AHEKWLPNFA DCIAELKKAH
Sequence cautioni
The sequence CAA37073 differs from that shown. Reason: Frameshift.Curated
The sequence CAA47257 differs from that shown. Reason: Frameshift.Curated
The sequence CAA47259 differs from that shown. Reason: Frameshift.Curated
Mass spectrometryi
Molecular mass is 50386 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52890 Genomic DNA Translation: CAA37073.1 Frameshift. U00096 Genomic DNA Translation: AAC74804.1 AP009048 Genomic DNA Translation: BAA15515.1 X66725 Genomic DNA Translation: CAA47257.1 Frameshift. X66725 Genomic DNA Translation: CAA47259.1 Frameshift. |
PIRi | F64932 |
RefSeqi | NP_416248.1, NC_000913.3 WP_000078765.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74804; AAC74804; b1734 BAA15515; BAA15515; BAA15515 |
GeneIDi | 946266 |
KEGGi | ecj:JW1723 eco:b1734 |
PATRICi | fig|1411691.4.peg.522 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52890 Genomic DNA Translation: CAA37073.1 Frameshift. U00096 Genomic DNA Translation: AAC74804.1 AP009048 Genomic DNA Translation: BAA15515.1 X66725 Genomic DNA Translation: CAA47257.1 Frameshift. X66725 Genomic DNA Translation: CAA47259.1 Frameshift. |
PIRi | F64932 |
RefSeqi | NP_416248.1, NC_000913.3 WP_000078765.1, NZ_LN832404.1 |
3D structure databases
SMRi | P17411 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259132, 399 interactors |
IntActi | P17411, 4 interactors |
STRINGi | 511145.b1734 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Proteomic databases
PaxDbi | P17411 |
PRIDEi | P17411 |
Genome annotation databases
EnsemblBacteriai | AAC74804; AAC74804; b1734 BAA15515; BAA15515; BAA15515 |
GeneIDi | 946266 |
KEGGi | ecj:JW1723 eco:b1734 |
PATRICi | fig|1411691.4.peg.522 |
Organism-specific databases
EchoBASEi | EB0142 |
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_0_1_6 |
InParanoidi | P17411 |
PhylomeDBi | P17411 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10144-MONOMER |
BRENDAi | 3.2.1.86, 2026 |
SABIO-RKi | P17411 |
Miscellaneous databases
PROi | PR:P17411 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHBF_ECOLI | |
Accessioni | P17411Primary (citable) accession number: P17411 Secondary accession number(s): P37795, P78290 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families