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Protein

Transcription factor AP-1

Gene

Jun

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei275Necessary for synergistic transcriptional activity with SMAD3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-2559580 Oxidative Stress Induced Senescence
R-RNO-2871796 FCERI mediated MAPK activation
R-RNO-450341 Activation of the AP-1 family of transcription factors
R-RNO-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor AP-1
Alternative name(s):
Activator protein 1
Short name:
AP1
Proto-oncogene c-Jun
V-jun avian sarcoma virus 17 oncogene homolog
Gene namesi
Name:Jun
Synonyms:Rjg-9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2943 Jun

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Chemistry databases

ChEMBLiCHEMBL3341579

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764321 – 334Transcription factor AP-1Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Phosphothreonine; by PAK2By similarity1
Modified residuei8Phosphothreonine; by PAK2By similarity1
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei56N6-acetyllysine; alternateBy similarity1
Cross-linki56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei58PhosphoserineBy similarity1
Modified residuei63Phosphoserine; by MAPK8 and PLK3By similarity1
Cross-linki70Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei73Phosphoserine; by MAPK8 and PLK3By similarity1
Modified residuei89Phosphothreonine; by PAK2By similarity1
Modified residuei91PhosphothreonineBy similarity1
Modified residuei93Phosphothreonine; by PAK2By similarity1
Cross-linki229Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei242Phosphothreonine; by GSK3-betaBy similarity1
Modified residuei246Phosphoserine; by DYRK2 and GSK3-betaBy similarity1
Modified residuei252Phosphoserine; by GSK3-betaBy similarity1
Modified residuei274N6-acetyllysineBy similarity1
Modified residuei289Phosphothreonine; by PAK2By similarity1

Post-translational modificationi

Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242. Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity (By similarity).By similarity
Ubiquitinated (PubMed:27458189). Ubiquitination by the SCF(FBXW7) is leading to its degradation (By similarity). Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7 (By similarity).By similarity1 Publication
Acetylated at Lys-271 by EP300.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP17325
PRIDEiP17325

PTM databases

iPTMnetiP17325
PhosphoSitePlusiP17325

Expressioni

Gene expression databases

BgeeiENSRNOG00000026293 Expressed in 9 organ(s), highest expression level in lung
GenevisibleiP17325 RN

Interactioni

Subunit structurei

Heterodimer with either FOS or BATF3 or ATF7 (PubMed:9154808). The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with MYBBP1A, SP1, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts synergistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex (By similarity). Binds to HIPK3 (By similarity). Interacts with methylated RNF187 (PubMed:20852630). Interacts (when phosphorylated) with FBXW7 (By similarity). Interacts with PRR7 (PubMed:27458189). Found in a complex with PRR7 and FBXW7 (By similarity). Interacts with PRR7 and FBXW7; the interaction inhibits ubiquitination-mediated JUN degradation promoting its phosphorylation and transcriptional activity (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk8P491852EBI-7709365,EBI-7456505

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246671, 6 interactors
CORUMiP17325
DIPiDIP-653N
IntActiP17325, 3 interactors
MINTiP17325
STRINGi10116.ENSRNOP00000011732

Chemistry databases

BindingDBiP17325

Structurei

3D structure databases

ProteinModelPortaliP17325
SMRiP17325
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini255 – 318bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni255 – 282Basic motifPROSITE-ProRule annotationAdd BLAST28
Regioni283 – 311Leucine-zipperPROSITE-ProRule annotationAdd BLAST29

Sequence similaritiesi

Belongs to the bZIP family. Jun subfamily.Curated

Phylogenomic databases

eggNOGiKOG0837 Eukaryota
ENOG410XRWH LUCA
GeneTreeiENSGT00390000009929
HOGENOMiHOG000006648
HOVERGENiHBG001722
InParanoidiP17325
KOiK04448
OMAiTFTYANM
OrthoDBiEOG091G0N0L
PhylomeDBiP17325
TreeFamiTF323952

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR015558 C_Jun/v-Jun
IPR005643 JNK
IPR002112 Leuzip_Jun
IPR008917 TF_DNA-bd_sf
PANTHERiPTHR11462:SF8 PTHR11462:SF8, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PF03957 Jun, 1 hit
PRINTSiPR00043 LEUZIPPRJUN
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequencei

Sequence statusi: Complete.

P17325-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAKMETTFY DDALNASFLQ SESGAYGYSN PKILKQSMTL NLADPVGNLK
60 70 80 90 100
PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP
110 120 130 140 150
KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVSGA GMVAPAVASV
160 170 180 190 200
AGAGGGGGYS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GATGLAFPSQ
210 220 230 240 250
PQQQQQPPQP PHHLPQQIPV QHPRLQALKE EPQTVPEMPG ETPPLSPIDM
260 270 280 290 300
ESQERIKAER KRMRNRIAAS KCRKRKLERI ARLEEKVKTL KAQNSELAST
310 320 330
ANMLREQVAQ LKQKVMNHVN SGCQLMLTQQ LQTF
Length:334
Mass (Da):36,001
Last modified:August 1, 1990 - v1
Checksum:iAFD9DF7D7C791B58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17163 mRNA Translation: CAA35041.1
X17215 Genomic DNA Translation: CAA35084.1
BC078738 mRNA Translation: AAH78738.1
PIRiS12742
RefSeqiNP_068607.1, NM_021835.3
UniGeneiRn.93714

Genome annotation databases

EnsembliENSRNOT00000011731; ENSRNOP00000011732; ENSRNOG00000026293
GeneIDi24516
KEGGirno:24516
UCSCiRGD:2943 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17163 mRNA Translation: CAA35041.1
X17215 Genomic DNA Translation: CAA35084.1
BC078738 mRNA Translation: AAH78738.1
PIRiS12742
RefSeqiNP_068607.1, NM_021835.3
UniGeneiRn.93714

3D structure databases

ProteinModelPortaliP17325
SMRiP17325
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246671, 6 interactors
CORUMiP17325
DIPiDIP-653N
IntActiP17325, 3 interactors
MINTiP17325
STRINGi10116.ENSRNOP00000011732

Chemistry databases

BindingDBiP17325
ChEMBLiCHEMBL3341579

PTM databases

iPTMnetiP17325
PhosphoSitePlusiP17325

Proteomic databases

PaxDbiP17325
PRIDEiP17325

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011731; ENSRNOP00000011732; ENSRNOG00000026293
GeneIDi24516
KEGGirno:24516
UCSCiRGD:2943 rat

Organism-specific databases

CTDi3725
RGDi2943 Jun

Phylogenomic databases

eggNOGiKOG0837 Eukaryota
ENOG410XRWH LUCA
GeneTreeiENSGT00390000009929
HOGENOMiHOG000006648
HOVERGENiHBG001722
InParanoidiP17325
KOiK04448
OMAiTFTYANM
OrthoDBiEOG091G0N0L
PhylomeDBiP17325
TreeFamiTF323952

Enzyme and pathway databases

ReactomeiR-RNO-2559580 Oxidative Stress Induced Senescence
R-RNO-2871796 FCERI mediated MAPK activation
R-RNO-450341 Activation of the AP-1 family of transcription factors
R-RNO-9018519 Estrogen-dependent gene expression

Miscellaneous databases

PROiPR:P17325

Gene expression databases

BgeeiENSRNOG00000026293 Expressed in 9 organ(s), highest expression level in lung
GenevisibleiP17325 RN

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR015558 C_Jun/v-Jun
IPR005643 JNK
IPR002112 Leuzip_Jun
IPR008917 TF_DNA-bd_sf
PANTHERiPTHR11462:SF8 PTHR11462:SF8, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PF03957 Jun, 1 hit
PRINTSiPR00043 LEUZIPPRJUN
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiJUN_RAT
AccessioniPrimary (citable) accession number: P17325
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 12, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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