UniProtKB - P17293 (RS12_THETH)
30S ribosomal protein S12
rpsL
Functioni
With S4 and S5 plays an important role in translational accuracy.
By similarityInteracts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).
By similarityGO - Molecular functioni
- rRNA binding Source: UniProtKB-UniRule
- structural constituent of ribosome Source: InterPro
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding |
Biological process | Antibiotic resistance |
Names & Taxonomyi
Protein namesi | Recommended name: 30S ribosomal protein S12 |
Gene namesi | Name:rpsL Synonyms:rps12 |
Organismi | Thermus thermophilus |
Taxonomic identifieri | 274 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000146342 | 2 – 132 | 30S ribosomal protein S12Add BLAST | 131 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 89 | 3-methylthioaspartic acid1 Publication | 1 |
Keywords - PTMi
MethylationInteractioni
Subunit structurei
Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity).
By similarityProtein-protein interaction databases
IntActi | P17293, 1 interactor |
MINTi | P17293 |
Structurei
Secondary structure
3D structure databases
SMRi | P17293 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17293 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 132 | DisorderedSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Family and domain databases
CDDi | cd03368, Ribosomal_S12, 1 hit |
HAMAPi | MF_00403_B, Ribosomal_S12_B, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR006032, Ribosomal_S12/S23 IPR005679, Ribosomal_S12_bac |
PANTHERi | PTHR11652, PTHR11652, 1 hit |
Pfami | View protein in Pfam PF00164, Ribosom_S12_S23, 1 hit |
PIRSFi | PIRSF002133, Ribosomal_S12/S23, 1 hit |
PRINTSi | PR01034, RIBOSOMALS12 |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00981, rpsL_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00055, RIBOSOMAL_S12, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MPTINQLVRK GREKVRKKSK VPALKGAPFR RGVCTVVRTV TPKKPNSALR
60 70 80 90 100
KVAKVRLTSG YEVTAYIPGE GHNLQEHSVV LIRGGRVKDL PGVRYHIVRG
110 120 130
VYDAAGVKDR KKSRSKYGTK KPKEAAKTAA KK
Mass spectrometryi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 42 | P → S in strain: Isolate HG18; streptomycin resistant. | 1 | |
Natural varianti | 43 | K → R in strain: Isolate HG3; streptomycin resistant. | 1 | |
Natural varianti | 86 | R → C in strain: Isolate HG14; streptomycin pseudo-dependent. | 1 | |
Natural varianti | 86 | R → H in strain: Isolate HG31; streptomycin pseudo-dependent. | 1 | |
Natural varianti | 88 | K → E in strain: Isolate HG19; streptomycin resistant. | 1 | |
Natural varianti | 88 | K → R in strain: Isolate HG1; streptomycin resistant. | 1 | |
Natural varianti | 91 | P → L in strain: Isolate HG11; streptomycin dependent. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF316617 Genomic DNA Translation: AAG38586.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF316617 Genomic DNA Translation: AAG38586.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2OM7 | electron microscopy | 7.30 | E | 1-132 | [»] | |
4V8X | X-ray | 3.35 | AL/CL | 1-132 | [»] | |
SMRi | P17293 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P17293, 1 interactor |
MINTi | P17293 |
Miscellaneous databases
EvolutionaryTracei | P17293 |
Family and domain databases
CDDi | cd03368, Ribosomal_S12, 1 hit |
HAMAPi | MF_00403_B, Ribosomal_S12_B, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR006032, Ribosomal_S12/S23 IPR005679, Ribosomal_S12_bac |
PANTHERi | PTHR11652, PTHR11652, 1 hit |
Pfami | View protein in Pfam PF00164, Ribosom_S12_S23, 1 hit |
PIRSFi | PIRSF002133, Ribosomal_S12/S23, 1 hit |
PRINTSi | PR01034, RIBOSOMALS12 |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00981, rpsL_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00055, RIBOSOMAL_S12, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RS12_THETH | |
Accessioni | P17293Primary (citable) accession number: P17293 Secondary accession number(s): Q9EYQ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 147 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families