UniProtKB - P17290 (TPH1_RABIT)
Protein
Tryptophan 5-hydroxylase 1
Gene
TPH1
Organism
Oryctolagus cuniculus (Rabbit)
Status
Functioni
Catalytic activityi
- (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + 5-hydroxy-L-tryptophanEC:1.14.16.4
Cofactori
: serotonin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes serotonin from L-tryptophan.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Tryptophan 5-monooxygenase (TPH2), Tryptophan 5-monooxygenase (TPH2), Tryptophan 5-hydroxylase 1 (TPH1), Tryptophan 5-monooxygenase (TPH2)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes serotonin from L-tryptophan, the pathway serotonin biosynthesis and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 235 | TryptophanBy similarity | 1 | |
Binding sitei | 257 | TryptophanBy similarity | 1 | |
Binding sitei | 265 | TryptophanBy similarity | 1 | |
Metal bindingi | 272 | IronBy similarity | 1 | |
Metal bindingi | 277 | IronBy similarity | 1 | |
Metal bindingi | 317 | IronBy similarity | 1 | |
Binding sitei | 336 | TryptophanBy similarity | 1 | |
Binding sitei | 366 | Tryptophan; via carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- iron ion binding Source: InterPro
- tryptophan 5-monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- aromatic amino acid family metabolic process Source: InterPro
- serotonin biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Serotonin biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
SABIO-RKi | P17290 |
UniPathwayi | UPA00846;UER00799 |
Names & Taxonomyi
Protein namesi | Recommended name: Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)Alternative name(s): Tryptophan 5-monooxygenase 1 |
Gene namesi | Name:TPH1 Synonyms:TPH |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205570 | 1 – 444 | Tryptophan 5-hydroxylase 1Add BLAST | 444 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 58 | Phosphoserine; by PKASequence analysis | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | P17290 |
PTM databases
iPTMneti | P17290 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 94 | ACTPROSITE-ProRule annotationAdd BLAST | 76 |
Sequence similaritiesi
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.Curated
Phylogenomic databases
InParanoidi | P17290 |
OrthoDBi | 614557at2759 |
Family and domain databases
CDDi | cd03346, eu_TrpOH, 1 hit |
Gene3Di | 1.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR002912, ACT_dom IPR001273, ArAA_hydroxylase IPR018301, ArAA_hydroxylase_Fe/CU_BS IPR036951, ArAA_hydroxylase_sf IPR036329, Aro-AA_hydroxylase_C_sf IPR019774, Aromatic-AA_hydroxylase_C IPR005963, Trp_5_mOase IPR041904, TrpOH_cat IPR019773, Tyrosine_3-monooxygenase-like |
PANTHERi | PTHR11473, PTHR11473, 1 hit |
Pfami | View protein in Pfam PF00351, Biopterin_H, 1 hit |
PIRSFi | PIRSF000336, TH, 1 hit |
PRINTSi | PR00372, FYWHYDRXLASE |
SUPFAMi | SSF56534, SSF56534, 1 hit |
TIGRFAMsi | TIGR01270, Trp_5_monoox, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00367, BH4_AAA_HYDROXYL_1, 1 hit PS51410, BH4_AAA_HYDROXYL_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P17290-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEDNKENKD HSLERGRATL IFSLKNEVGG LIKALKIFQE KHVNLLHIES
60 70 80 90 100
RKSKRRNSEF EIFVDCDTNR EQLNDIFHLL KSHTNVLSVT PPDNFTMKEE
110 120 130 140 150
GMESVPWFPK KISDLDHCAN RVLMYGSELD ADHPGFKDNV YRKRRKYFAD
160 170 180 190 200
LAMSYKYGDP IPKVEFTEEE IKTWGTVFRE LNKLYPTHAC REYLKNLPLL
210 220 230 240 250
SKYCGYREDN IPQLEDISNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF
260 270 280 290 300
HCTQYVRHSS DPFYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA
310 320 330 340 350
SEEAVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LKHVLSGHAK
360 370 380 390 400
VKPFDPKITY KQECLITTFQ DVYFVSESFE DAKEKMREFT KTIKRPFGVK
410 420 430 440
YNPYTRSIQI LKDAKSITNA MNELRHDLDV VSDALGKVSR QLSV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | M → L in AAA31487 (PubMed:3475690).Curated | 1 | |
Sequence conflicti | 151 | L → S in AAA67051 (PubMed:7986090).Curated | 1 | |
Sequence conflicti | 202 – 203 | KY → ND in AAA31487 (PubMed:3475690).Curated | 2 | |
Sequence conflicti | 207 | R → Q in AAA67051 (PubMed:7986090).Curated | 1 | |
Sequence conflicti | 390 | T → K in AAA31487 (PubMed:3475690).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17250 mRNA Translation: AAA31487.1 L29305 mRNA Translation: AAA67051.1 |
PIRi | S51199 |
RefSeqi | NP_001075741.1, NM_001082272.1 NP_001093425.1, NM_001099955.1 |
Genome annotation databases
GeneIDi | 100009100 100101558 |
KEGGi | ocu:100009100 ocu:100101558 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17250 mRNA Translation: AAA31487.1 L29305 mRNA Translation: AAA67051.1 |
PIRi | S51199 |
RefSeqi | NP_001075741.1, NM_001082272.1 NP_001093425.1, NM_001099955.1 |
3D structure databases
SMRi | P17290 |
ModBasei | Search... |
PTM databases
iPTMneti | P17290 |
Proteomic databases
PRIDEi | P17290 |
Genome annotation databases
GeneIDi | 100009100 100101558 |
KEGGi | ocu:100009100 ocu:100101558 |
Organism-specific databases
CTDi | 7166 |
Phylogenomic databases
InParanoidi | P17290 |
OrthoDBi | 614557at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00846;UER00799 |
SABIO-RKi | P17290 |
Family and domain databases
CDDi | cd03346, eu_TrpOH, 1 hit |
Gene3Di | 1.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR002912, ACT_dom IPR001273, ArAA_hydroxylase IPR018301, ArAA_hydroxylase_Fe/CU_BS IPR036951, ArAA_hydroxylase_sf IPR036329, Aro-AA_hydroxylase_C_sf IPR019774, Aromatic-AA_hydroxylase_C IPR005963, Trp_5_mOase IPR041904, TrpOH_cat IPR019773, Tyrosine_3-monooxygenase-like |
PANTHERi | PTHR11473, PTHR11473, 1 hit |
Pfami | View protein in Pfam PF00351, Biopterin_H, 1 hit |
PIRSFi | PIRSF000336, TH, 1 hit |
PRINTSi | PR00372, FYWHYDRXLASE |
SUPFAMi | SSF56534, SSF56534, 1 hit |
TIGRFAMsi | TIGR01270, Trp_5_monoox, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00367, BH4_AAA_HYDROXYL_1, 1 hit PS51410, BH4_AAA_HYDROXYL_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TPH1_RABIT | |
Accessioni | P17290Primary (citable) accession number: P17290 Secondary accession number(s): Q29523 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families