UniProtKB - P17282 (GAG_SIVCZ)
Protein
Gag polyprotein
Gene
gag
Organism
Simian immunodeficiency virus (isolate CPZ GAB1) (SIV-cpz) (Chimpanzee immunodeficiency virus)
Status
Functioni
Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu (By similarity).By similarity
Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).By similarity
p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 400 – 417 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 421 – 438 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- structural molecule activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- viral budding via host ESCRT complex Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding, Viral nucleoprotein |
Biological process | Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Gag polyproteinAlternative name(s): Pr55Gag Cleaved into the following 6 chains: |
Gene namesi | Name:gag |
Organismi | Simian immunodeficiency virus (isolate CPZ GAB1) (SIV-cpz) (Chimpanzee immunodeficiency virus) |
Taxonomic identifieri | 402771 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Pararnavirae › Artverviricota › Revtraviricetes › Ortervirales › Retroviridae › Orthoretrovirinae › Lentivirus › |
Virus hosti | Pan (chimpanzees) [TaxID: 9596] |
Proteomesi |
|
Subcellular locationi
- Virion Curated
- Host nucleus By similarity
- Host cytoplasm By similarity
- Host cell membrane Curated; Lipid-anchor Curated Note: Following virus entry, the nuclear localization signal (NLS) of the matrix protein participates with Vpr to the nuclear localization of the viral genome. During virus production, the nuclear export activity of the matrix protein counteracts the NLS to maintain the Gag and Gag-Pol polyproteins in the cytoplasm, thereby directing unspliced RNA to the plasma membrane (By similarity).By similarity
- Virion Curated
- Virion Curated
Keywords - Cellular componenti
Capsid protein, Host cell membrane, Host cytoplasm, Host membrane, Host nucleus, Membrane, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed; by hostBy similarity | |||
ChainiPRO_0000316150 | 2 – 508 | Gag polyproteinBy similarityAdd BLAST | 507 | |
ChainiPRO_0000038644 | 2 – 140 | Matrix protein p17By similarityAdd BLAST | 139 | |
ChainiPRO_0000038645 | 141 – 371 | Capsid protein p24By similarityAdd BLAST | 231 | |
PeptideiPRO_0000316151 | 372 – 387 | Spacer peptide p2By similarityAdd BLAST | 16 | |
ChainiPRO_0000038646 | 388 – 442 | Nucleocapsid protein p7By similarityAdd BLAST | 55 | |
PeptideiPRO_0000316152 | 443 – 458 | Spacer peptide p1By similarityAdd BLAST | 16 | |
ChainiPRO_0000316153 | 459 – 508 | p6-gagBy similarityAdd BLAST | 50 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine; by hostBy similarity | 1 |
Post-translational modificationi
Capsid protein p24 is phosphorylated.By similarity
Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 140 – 141 | Cleavage; by viral proteaseBy similarity | 2 | |
Sitei | 371 – 372 | Cleavage; by viral proteaseBy similarity | 2 | |
Sitei | 387 – 388 | Cleavage; by viral proteaseBy similarity | 2 | |
Sitei | 442 – 443 | Cleavage; by viral proteaseBy similarity | 2 | |
Sitei | 458 – 459 | Cleavage; by viral proteaseBy similarity | 2 |
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinFamily & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 16 – 22 | Nuclear export signalBy similarity | 7 | |
Motifi | 26 – 32 | Nuclear localization signalBy similarity | 7 | |
Motifi | 465 – 468 | PTAP/PSAP motif | 4 | |
Motifi | 491 – 500 | LYPX(n)L motif | 10 |
Domaini
Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. p6-gag contains two L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a LYPX(n)L motif which interacts with PDCD6IP/AIP1 (By similarity).By similarity
Sequence similaritiesi
Belongs to the primate lentivirus group gag polyprotein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 400 – 417 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 421 – 438 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
Keywords - Domaini
Repeat, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.1200.30, 1 hit 1.10.150.90, 1 hit 1.10.375.10, 1 hit |
InterProi | View protein in InterPro IPR000721, Gag_p24 IPR014817, Gag_p6 IPR000071, Lentvrl_matrix_N IPR012344, Matrix_HIV/RSV_N IPR008916, Retrov_capsid_C IPR008919, Retrov_capsid_N IPR010999, Retrovr_matrix IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF00540, Gag_p17, 1 hit PF00607, Gag_p24, 1 hit PF08705, Gag_p6, 1 hit PF00098, zf-CCHC, 2 hits |
PRINTSi | PR00234, HIV1MATRIX |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF47836, SSF47836, 1 hit SSF47943, SSF47943, 1 hit SSF57756, SSF57756, 1 hit |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketNote: Translation results in the formation of the Gag polyprotein most of the time. Ribosomal frameshifting at the gag-pol genes boundary occurs at low frequency and produces the Gag-Pol polyprotein. This strategy of translation probably allows the virus to modulate the quantity of each viral protein. Maintenance of a correct Gag to Gag-Pol ratio is essential for RNA dimerization and viral infectivity.
Isoform Gag polyprotein (identifier: P17282-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGARASVLTG GKLDRWEKVR LRPGGRKRYM MKHLVWASRE LERFACDPGL
60 70 80 90 100
MESKEGCTKL LQQLEPALKT GSEGLRSLFN TLAVLWCIHS DITVEDTQKA
110 120 130 140 150
LEQLKRHHGE QQSKTESNSG SREGGASQGA SASAGISGNY PLVQNAQGQM
160 170 180 190 200
VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGALPQ DVNTMLNAVG
210 220 230 240 250
GHQGAMQVLK EVINEEAAEW DRLHPTHAGP IAPGQLREPR GSDIAGTTST
260 270 280 290 300
LQEQIGWTTA NPPIPVGDVY RRWVILGLNK VVRMYCPVSI LDIRQGPKEP
310 320 330 340 350
FRDYVDRFYK TLRAEQASQE VKNWMTDTLL VQNANPDCKQ ILKALGPGAT
360 370 380 390 400
LEEMMTACQG VGGPSHKARV LAEAMSMVQN QGRADVFFQK GQGAGPKRKI
410 420 430 440 450
KCFNCGKEGH LARNCKAPRR KGCWRCGQEG HQMKDCTGRQ VNFLGKGWPS
460 470 480 490 500
RSGRPGNFVQ NRTEPTAPPI ESYGYQEEEK SQEKKEGESS LYPPTSLKSL
FGSDPSSQ
Note: Produced by conventional translation.
Isoform Gag-Pol polyprotein (identifier: P17283-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P17283.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52154 Genomic RNA Translation: CAA36401.1 |
PIRi | S09983, FOLJSI |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52154 Genomic RNA Translation: CAA36401.1 |
PIRi | S09983, FOLJSI |
3D structure databases
SMRi | P17282 |
ModBasei | Search... |
Protein-protein interaction databases
ELMi | P17282 |
Miscellaneous databases
PROi | PR:P17282 |
Family and domain databases
Gene3Di | 1.10.1200.30, 1 hit 1.10.150.90, 1 hit 1.10.375.10, 1 hit |
InterProi | View protein in InterPro IPR000721, Gag_p24 IPR014817, Gag_p6 IPR000071, Lentvrl_matrix_N IPR012344, Matrix_HIV/RSV_N IPR008916, Retrov_capsid_C IPR008919, Retrov_capsid_N IPR010999, Retrovr_matrix IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF00540, Gag_p17, 1 hit PF00607, Gag_p24, 1 hit PF08705, Gag_p6, 1 hit PF00098, zf-CCHC, 2 hits |
PRINTSi | PR00234, HIV1MATRIX |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF47836, SSF47836, 1 hit SSF47943, SSF47943, 1 hit SSF57756, SSF57756, 1 hit |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GAG_SIVCZ | |
Accessioni | P17282Primary (citable) accession number: P17282 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | October 7, 2020 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families