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Protein

Proteasome subunit alpha type-2

Gene

Psma2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.972

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-2 (EC:3.4.25.1By similarity)
Alternative name(s):
Macropain subunit C3
Multicatalytic endopeptidase complex subunit C3
Proteasome component C3
Gene namesi
Name:Psma2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi61842 Psma2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi121Y → F: Abolishes nuclear localization and phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001240792 – 234Proteasome subunit alpha type-2Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei6PhosphotyrosineBy similarity1
Modified residuei7PhosphoserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei24PhosphotyrosineBy similarity1
Modified residuei70N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineBy similarity1
Modified residuei121Phosphotyrosine1 Publication1
Modified residuei171N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues; which may be important for nuclear import.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP17220
PRIDEiP17220

2D gel databases

World-2DPAGEi0004:P17220

PTM databases

iPTMnetiP17220
PhosphoSitePlusiP17220

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSRNOG00000049920 Expressed in 10 organ(s), highest expression level in colon
GenevisibleiP17220 RN

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.By similarity

Protein-protein interaction databases

BioGridi248290, 5 interactors
IntActiP17220, 1 interactor
STRINGi10116.ENSRNOP00000066950

Structurei

3D structure databases

ProteinModelPortaliP17220
SMRiP17220
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0181 Eukaryota
ENOG410XPQ8 LUCA
GeneTreeiENSGT00550000074870
HOVERGENiHBG003005
InParanoidiP17220
KOiK02726
OMAiFAWKASA
OrthoDBiEOG091G0GX6
PhylomeDBiP17220

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
IPR034644 Proteasome_subunit_alpha2
PANTHERiPTHR11599:SF16 PTHR11599:SF16, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17220-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK
60 70 80 90 100
KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV
110 120 130 140 150
YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD
160 170 180 190 200
PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE
210 220 230
GQMTEDNIEV GICNEAGFRR LTPTEVRDYL AAIA
Length:234
Mass (Da):25,927
Last modified:January 23, 2007 - v3
Checksum:i4ECB56A233583821
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02897 mRNA Translation: AAA40838.1
PIRiA34535 SNRTC3
RefSeqiNP_058975.1, NM_017279.1
UniGeneiRn.1617

Genome annotation databases

EnsembliENSRNOT00000073834; ENSRNOP00000066950; ENSRNOG00000049920
GeneIDi29669
KEGGirno:29669
UCSCiRGD:61842 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02897 mRNA Translation: AAA40838.1
PIRiA34535 SNRTC3
RefSeqiNP_058975.1, NM_017279.1
UniGeneiRn.1617

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6EPCelectron microscopy12.30B1-234[»]
6EPDelectron microscopy15.40B1-234[»]
6EPEelectron microscopy12.80B1-234[»]
6EPFelectron microscopy11.80B1-234[»]
ProteinModelPortaliP17220
SMRiP17220
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248290, 5 interactors
IntActiP17220, 1 interactor
STRINGi10116.ENSRNOP00000066950

Protein family/group databases

MEROPSiT01.972

PTM databases

iPTMnetiP17220
PhosphoSitePlusiP17220

2D gel databases

World-2DPAGEi0004:P17220

Proteomic databases

PaxDbiP17220
PRIDEiP17220

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073834; ENSRNOP00000066950; ENSRNOG00000049920
GeneIDi29669
KEGGirno:29669
UCSCiRGD:61842 rat

Organism-specific databases

CTDi5683
RGDi61842 Psma2

Phylogenomic databases

eggNOGiKOG0181 Eukaryota
ENOG410XPQ8 LUCA
GeneTreeiENSGT00550000074870
HOVERGENiHBG003005
InParanoidiP17220
KOiK02726
OMAiFAWKASA
OrthoDBiEOG091G0GX6
PhylomeDBiP17220

Miscellaneous databases

PROiPR:P17220

Gene expression databases

BgeeiENSRNOG00000049920 Expressed in 10 organ(s), highest expression level in colon
GenevisibleiP17220 RN

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
IPR034644 Proteasome_subunit_alpha2
PANTHERiPTHR11599:SF16 PTHR11599:SF16, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSA2_RAT
AccessioniPrimary (citable) accession number: P17220
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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