UniProtKB - P17220 (PSA2_RAT)
Proteasome subunit alpha type-2
Psma2
Functioni
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
By similarityGO - Biological processi
- proteasomal protein catabolic process Source: GO_Central
- response to virus Source: RGD
- ubiquitin-dependent protein catabolic process Source: InterPro
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-2467813, Separation of Sister Chromatids R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-6798695, Neutrophil degranulation R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8932339, ROS sensing by NFE2L2 R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | T01.972 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome subunit alpha type-2Alternative name(s): Macropain subunit C3 Multicatalytic endopeptidase complex subunit C3 Proteasome component C3 |
Gene namesi | Name:Psma2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61842, Psma2 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Translocated from the cytoplasm into the nucleus following interaction with AKIRIN2, which bridges the proteasome with the nuclear import receptor IPO9 (By similarity). Colocalizes with TRIM5 in cytoplasmic bodies (By similarity).By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- P-body Source: UniProtKB
- proteasome complex Source: RGD
- proteasome core complex Source: UniProtKB
- proteasome core complex, alpha-subunit complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 121 | Y → F: Abolishes nuclear localization and phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000124079 | 2 – 234 | Proteasome subunit alpha type-2Add BLAST | 233 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 6 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 7 | PhosphoserineBy similarity | 1 | |
Modified residuei | 14 | PhosphoserineBy similarity | 1 | |
Modified residuei | 16 | PhosphoserineBy similarity | 1 | |
Modified residuei | 24 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 70 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 76 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 121 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 171 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P17220 |
PaxDbi | P17220 |
PRIDEi | P17220 |
2D gel databases
World-2DPAGEi | 0004:P17220 |
PTM databases
iPTMneti | P17220 |
PhosphoSitePlusi | P17220 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000049920, Expressed in thymus and 22 other tissues |
Genevisiblei | P17220, RN |
Interactioni
Subunit structurei
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.
By similarityProtein-protein interaction databases
BioGRIDi | 248290, 6 interactors |
IntActi | P17220, 2 interactors |
STRINGi | 10116.ENSRNOP00000066950 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P17220 |
SMRi | P17220 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0181, Eukaryota |
GeneTreei | ENSGT00550000074870 |
HOGENOMi | CLU_035750_4_1_1 |
InParanoidi | P17220 |
OMAi | YQEQIPT |
OrthoDBi | 1222564at2759 |
PhylomeDBi | P17220 |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR000426, Proteasome_asu_N IPR001353, Proteasome_sua/b IPR034644, Proteasome_subunit_alpha2 |
PANTHERi | PTHR11599:SF16, PTHR11599:SF16, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK
60 70 80 90 100
KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV
110 120 130 140 150
YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD
160 170 180 190 200
PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE
210 220 230
GQMTEDNIEV GICNEAGFRR LTPTEVRDYL AAIA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A8I5ZV22 | A0A8I5ZV22_RAT | Proteasome subunit alpha type-2 | Psma2 | 233 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02897 mRNA Translation: AAA40838.1 |
PIRi | A34535, SNRTC3 |
RefSeqi | NP_058975.1, NM_017279.1 |
Genome annotation databases
Ensembli | ENSRNOT00000073834; ENSRNOP00000066950; ENSRNOG00000049920 |
GeneIDi | 29669 |
KEGGi | rno:29669 |
UCSCi | RGD:61842, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02897 mRNA Translation: AAA40838.1 |
PIRi | A34535, SNRTC3 |
RefSeqi | NP_058975.1, NM_017279.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EPC | electron microscopy | 12.30 | B | 1-234 | [»] | |
6EPD | electron microscopy | 15.40 | B | 1-234 | [»] | |
6EPE | electron microscopy | 12.80 | B | 1-234 | [»] | |
6EPF | electron microscopy | 11.80 | B | 1-234 | [»] | |
6TU3 | electron microscopy | 2.70 | B/P | 1-234 | [»] | |
AlphaFoldDBi | P17220 | |||||
SMRi | P17220 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248290, 6 interactors |
IntActi | P17220, 2 interactors |
STRINGi | 10116.ENSRNOP00000066950 |
Protein family/group databases
MEROPSi | T01.972 |
PTM databases
iPTMneti | P17220 |
PhosphoSitePlusi | P17220 |
2D gel databases
World-2DPAGEi | 0004:P17220 |
Proteomic databases
jPOSTi | P17220 |
PaxDbi | P17220 |
PRIDEi | P17220 |
Genome annotation databases
Ensembli | ENSRNOT00000073834; ENSRNOP00000066950; ENSRNOG00000049920 |
GeneIDi | 29669 |
KEGGi | rno:29669 |
UCSCi | RGD:61842, rat |
Organism-specific databases
CTDi | 5683 |
RGDi | 61842, Psma2 |
Phylogenomic databases
eggNOGi | KOG0181, Eukaryota |
GeneTreei | ENSGT00550000074870 |
HOGENOMi | CLU_035750_4_1_1 |
InParanoidi | P17220 |
OMAi | YQEQIPT |
OrthoDBi | 1222564at2759 |
PhylomeDBi | P17220 |
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-2467813, Separation of Sister Chromatids R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-6798695, Neutrophil degranulation R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8932339, ROS sensing by NFE2L2 R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P17220 |
Gene expression databases
Bgeei | ENSRNOG00000049920, Expressed in thymus and 22 other tissues |
Genevisiblei | P17220, RN |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR000426, Proteasome_asu_N IPR001353, Proteasome_sua/b IPR034644, Proteasome_subunit_alpha2 |
PANTHERi | PTHR11599:SF16, PTHR11599:SF16, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PSA2_RAT | |
Accessioni | P17220Primary (citable) accession number: P17220 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 179 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families