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UniProtKB - P17169 (GLMS_ECOLI)
Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
glmS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.
Catalytic activityi
- EC:2.6.1.16
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | Nucleophile; for GATase activityBy similarity | 1 | |
Active sitei | 604 | For Fru-6P isomerization activity | 1 |
GO - Molecular functioni
- carbohydrate derivative binding Source: InterPro
- glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: EcoCyc
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-UniRule
- fructose 6-phosphate metabolic process Source: GO_Central
- glutamine metabolic process Source: UniProtKB-KW
- protein N-linked glycosylation Source: GO_Central
- UDP-N-acetylglucosamine biosynthetic process Source: EcoCyc
- UDP-N-acetylglucosamine metabolic process Source: GO_Central
Keywordsi
Molecular function | Aminotransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:L-GLN-FRUCT-6-P-AMINOTRANS-MONOMER |
BRENDAi | 2.6.1.16, 2026 |
SABIO-RKi | P17169 |
Protein family/group databases
MEROPSi | C44.971 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)Alternative name(s): D-fructose-6-phosphate amidotransferase GFAT Glucosamine-6-phosphate synthase Hexosephosphate aminotransferase L-glutamine--D-fructose-6-phosphate amidotransferase |
Gene namesi | Name:glmS Ordered Locus Names:b3729, JW3707 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid DB02445, 2-Deoxy-2-Amino Glucitol-6-Phosphate DB02007, alpha-D-glucose 6-phosphate DB02657, Glucosamine 6-Phosphate DB03581, Glucose-6-Phosphate DB02446, Glutamine hydroxamate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000135328 | 2 – 609 | Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST | 608 |
Proteomic databases
jPOSTi | P17169 |
PaxDbi | P17169 |
PRIDEi | P17169 |
Expressioni
Inductioni
Post-transcriptionally regulated by the GlmY/GlmZ sRNA regulatory cascade. The sRNA GlmZ, together with Hfq, directly activates glmS mRNA translation through base-pairing. A second sRNA, GlmY, positively regulates glmS indirectly, by sequestering the adapter protein RapZ and protecting GlmZ from RNA cleavage.3 Publications
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4262136, 528 interactors 852543, 1 interactor |
DIPi | DIP-9775N |
IntActi | P17169, 8 interactors |
STRINGi | 511145.b3729 |
Chemistry databases
BindingDBi | P17169 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P17169 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17169 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 218 | Glutamine amidotransferase type-2Add BLAST | 217 | |
Domaini | 286 – 426 | SIS 1Add BLAST | 141 | |
Domaini | 458 – 599 | SIS 2Add BLAST | 142 |
Keywords - Domaini
Glutamine amidotransferase, RepeatPhylogenomic databases
eggNOGi | COG0449, Bacteria |
HOGENOMi | CLU_012520_5_2_6 |
InParanoidi | P17169 |
PhylomeDBi | P17169 |
Family and domain databases
CDDi | cd05008, SIS_GlmS_GlmD_1, 1 hit cd05009, SIS_GlmS_GlmD_2, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
HAMAPi | MF_00164, GlmS, 1 hit |
InterProi | View protein in InterPro IPR017932, GATase_2_dom IPR035466, GlmS/AgaS_SIS IPR035490, GlmS/FrlB_SIS IPR005855, GlmS_trans IPR029055, Ntn_hydrolases_N IPR001347, SIS_dom |
PANTHERi | PTHR10937:SF0, PTHR10937:SF0, 1 hit |
Pfami | View protein in Pfam PF01380, SIS, 2 hits |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01135, glmS, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit PS51464, SIS, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P17169-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCGIVGAIAQ RDVAEILLEG LRRLEYRGYD SAGLAVVDAE GHMTRLRRLG
60 70 80 90 100
KVQMLAQAAE EHPLHGGTGI AHTRWATHGE PSEVNAHPHV SEHIVVVHNG
110 120 130 140 150
IIENHEPLRE ELKARGYTFV SETDTEVIAH LVNWELKQGG TLREAVLRAI
160 170 180 190 200
PQLRGAYGTV IMDSRHPDTL LAARSGSPLV IGLGMGENFI ASDQLALLPV
210 220 230 240 250
TRRFIFLEEG DIAEITRRSV NIFDKTGAEV KRQDIESNLQ YDAGDKGIYR
260 270 280 290 300
HYMQKEIYEQ PNAIKNTLTG RISHGQVDLS ELGPNADELL SKVEHIQILA
310 320 330 340 350
CGTSYNSGMV SRYWFESLAG IPCDVEIASE FRYRKSAVRR NSLMITLSQS
360 370 380 390 400
GETADTLAGL RLSKELGYLG SLAICNVPGS SLVRESDLAL MTNAGTEIGV
410 420 430 440 450
ASTKAFTTQL TVLLMLVAKL SRLKGLDASI EHDIVHGLQA LPSRIEQMLS
460 470 480 490 500
QDKRIEALAE DFSDKHHALF LGRGDQYPIA LEGALKLKEI SYIHAEAYAA
510 520 530 540 550
GELKHGPLAL IDADMPVIVV APNNELLEKL KSNIEEVRAR GGQLYVFADQ
560 570 580 590 600
DAGFVSSDNM HIIEMPHVEE VIAPIFYTVP LQLLAYHVAL IKGTDVDQPR
NLAKSVTVE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 419 – 420 | KL → NV in CAA25785 (PubMed:6395859).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01631 Genomic DNA Translation: CAA25785.1 L10328 Genomic DNA Translation: AAA62080.1 U00096 Genomic DNA Translation: AAC76752.1 AP009048 Genomic DNA Translation: BAE77559.1 V00620 Genomic DNA Translation: CAA23894.1 M18980 Genomic DNA Translation: AAA23836.1 |
PIRi | B65176, XNECGM |
RefSeqi | NP_418185.1, NC_000913.3 WP_000334099.1, NZ_SSZK01000036.1 |
Genome annotation databases
EnsemblBacteriai | AAC76752; AAC76752; b3729 BAE77559; BAE77559; BAE77559 |
GeneIDi | 66672367 948241 |
KEGGi | ecj:JW3707 eco:b3729 |
PATRICi | fig|1411691.4.peg.2971 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01631 Genomic DNA Translation: CAA25785.1 L10328 Genomic DNA Translation: AAA62080.1 U00096 Genomic DNA Translation: AAC76752.1 AP009048 Genomic DNA Translation: BAE77559.1 V00620 Genomic DNA Translation: CAA23894.1 M18980 Genomic DNA Translation: AAA23836.1 |
PIRi | B65176, XNECGM |
RefSeqi | NP_418185.1, NC_000913.3 WP_000334099.1, NZ_SSZK01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JXA | X-ray | 3.10 | A/B/C | 2-609 | [»] | |
1MOQ | X-ray | 1.57 | A | 242-609 | [»] | |
1MOR | X-ray | 1.90 | A | 242-609 | [»] | |
1MOS | X-ray | 2.00 | A | 242-609 | [»] | |
1XFF | X-ray | 1.80 | A/B | 2-241 | [»] | |
1XFG | X-ray | 1.85 | A/B | 2-241 | [»] | |
2J6H | X-ray | 2.35 | A/B | 2-609 | [»] | |
2VF4 | X-ray | 2.95 | X | 2-609 | [»] | |
2VF5 | X-ray | 2.90 | X | 2-609 | [»] | |
3OOJ | X-ray | 2.50 | A/B/C/D/E/F/G/H | 3-609 | [»] | |
4AMV | X-ray | 2.05 | A/B/C | 1-609 | [»] | |
SMRi | P17169 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262136, 528 interactors 852543, 1 interactor |
DIPi | DIP-9775N |
IntActi | P17169, 8 interactors |
STRINGi | 511145.b3729 |
Chemistry databases
BindingDBi | P17169 |
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid DB02445, 2-Deoxy-2-Amino Glucitol-6-Phosphate DB02007, alpha-D-glucose 6-phosphate DB02657, Glucosamine 6-Phosphate DB03581, Glucose-6-Phosphate DB02446, Glutamine hydroxamate |
Protein family/group databases
MEROPSi | C44.971 |
Proteomic databases
jPOSTi | P17169 |
PaxDbi | P17169 |
PRIDEi | P17169 |
Genome annotation databases
EnsemblBacteriai | AAC76752; AAC76752; b3729 BAE77559; BAE77559; BAE77559 |
GeneIDi | 66672367 948241 |
KEGGi | ecj:JW3707 eco:b3729 |
PATRICi | fig|1411691.4.peg.2971 |
Organism-specific databases
EchoBASEi | EB0377 |
Phylogenomic databases
eggNOGi | COG0449, Bacteria |
HOGENOMi | CLU_012520_5_2_6 |
InParanoidi | P17169 |
PhylomeDBi | P17169 |
Enzyme and pathway databases
BioCyci | EcoCyc:L-GLN-FRUCT-6-P-AMINOTRANS-MONOMER |
BRENDAi | 2.6.1.16, 2026 |
SABIO-RKi | P17169 |
Miscellaneous databases
EvolutionaryTracei | P17169 |
PROi | PR:P17169 |
Family and domain databases
CDDi | cd05008, SIS_GlmS_GlmD_1, 1 hit cd05009, SIS_GlmS_GlmD_2, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
HAMAPi | MF_00164, GlmS, 1 hit |
InterProi | View protein in InterPro IPR017932, GATase_2_dom IPR035466, GlmS/AgaS_SIS IPR035490, GlmS/FrlB_SIS IPR005855, GlmS_trans IPR029055, Ntn_hydrolases_N IPR001347, SIS_dom |
PANTHERi | PTHR10937:SF0, PTHR10937:SF0, 1 hit |
Pfami | View protein in Pfam PF01380, SIS, 2 hits |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01135, glmS, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit PS51464, SIS, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GLMS_ECOLI | |
Accessioni | P17169Primary (citable) accession number: P17169 Secondary accession number(s): P76745, Q2M847 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 206 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references