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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityBy similarity1
Active sitei604For Fru-6P isomerization activity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-UniRule
  • fructose 6-phosphate metabolic process Source: GO_Central
  • glutamine metabolic process Source: UniProtKB-KW
  • protein N-linked glycosylation Source: GO_Central
  • UDP-N-acetylglucosamine biosynthetic process Source: EcoCyc
  • UDP-N-acetylglucosamine metabolic process Source: GO_Central

Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:L-GLN-FRUCT-6-P-AMINOTRANS-MONOMER
MetaCyc:L-GLN-FRUCT-6-P-AMINOTRANS-MONOMER
BRENDAi2.6.1.16 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene namesi
Name:glmS
Ordered Locus Names:b3729, JW3707
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10382 glmS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid
DB02445 2-Deoxy-2-Amino Glucitol-6-Phosphate
DB02007 alpha-D-glucose 6-phosphate
DB02657 Glucosamine 6-Phosphate
DB03581 Glucose-6-Phosphate
DB02446 Glutamine Hydroxamate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001353282 – 609Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST608

Proteomic databases

EPDiP17169
PaxDbiP17169
PRIDEiP17169

Expressioni

Inductioni

Post-transcriptionally regulated by the GlmY/GlmZ sRNA regulatory cascade. The sRNA GlmZ, together with Hfq, directly activates glmS mRNA translation through base-pairing. A second sRNA, GlmY, positively regulates glmS indirectly, by sequestering the adapter protein RapZ and protecting GlmZ from RNA cleavage.3 Publications

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Protein-protein interaction databases

BioGridi4262136, 528 interactors
DIPiDIP-9775N
IntActiP17169, 8 interactors
STRINGi316407.85676309

Chemistry databases

BindingDBiP17169

Structurei

Secondary structure

1609
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP17169
SMRiP17169
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17169

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 218Glutamine amidotransferase type-2Add BLAST217
Domaini286 – 426SIS 1Add BLAST141
Domaini458 – 599SIS 2Add BLAST142

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiENOG4105C46 Bacteria
COG0449 LUCA
HOGENOMiHOG000258898
InParanoidiP17169
KOiK00820
PhylomeDBiP17169

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
Gene3Di3.60.20.10, 1 hit
HAMAPiMF_00164 GlmS, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PANTHERiPTHR10937:SF0 PTHR10937:SF0, 1 hit
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17169-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGIVGAIAQ RDVAEILLEG LRRLEYRGYD SAGLAVVDAE GHMTRLRRLG
60 70 80 90 100
KVQMLAQAAE EHPLHGGTGI AHTRWATHGE PSEVNAHPHV SEHIVVVHNG
110 120 130 140 150
IIENHEPLRE ELKARGYTFV SETDTEVIAH LVNWELKQGG TLREAVLRAI
160 170 180 190 200
PQLRGAYGTV IMDSRHPDTL LAARSGSPLV IGLGMGENFI ASDQLALLPV
210 220 230 240 250
TRRFIFLEEG DIAEITRRSV NIFDKTGAEV KRQDIESNLQ YDAGDKGIYR
260 270 280 290 300
HYMQKEIYEQ PNAIKNTLTG RISHGQVDLS ELGPNADELL SKVEHIQILA
310 320 330 340 350
CGTSYNSGMV SRYWFESLAG IPCDVEIASE FRYRKSAVRR NSLMITLSQS
360 370 380 390 400
GETADTLAGL RLSKELGYLG SLAICNVPGS SLVRESDLAL MTNAGTEIGV
410 420 430 440 450
ASTKAFTTQL TVLLMLVAKL SRLKGLDASI EHDIVHGLQA LPSRIEQMLS
460 470 480 490 500
QDKRIEALAE DFSDKHHALF LGRGDQYPIA LEGALKLKEI SYIHAEAYAA
510 520 530 540 550
GELKHGPLAL IDADMPVIVV APNNELLEKL KSNIEEVRAR GGQLYVFADQ
560 570 580 590 600
DAGFVSSDNM HIIEMPHVEE VIAPIFYTVP LQLLAYHVAL IKGTDVDQPR

NLAKSVTVE
Length:609
Mass (Da):66,894
Last modified:January 23, 2007 - v4
Checksum:iA1CB929E839436E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti419 – 420KL → NV in CAA25785 (PubMed:6395859).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA Translation: CAA25785.1
L10328 Genomic DNA Translation: AAA62080.1
U00096 Genomic DNA Translation: AAC76752.1
AP009048 Genomic DNA Translation: BAE77559.1
V00620 Genomic DNA Translation: CAA23894.1
M18980 Genomic DNA Translation: AAA23836.1
PIRiB65176 XNECGM
RefSeqiNP_418185.1, NC_000913.3
WP_000334099.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76752; AAC76752; b3729
BAE77559; BAE77559; BAE77559
GeneIDi948241
KEGGiecj:JW3707
eco:b3729
PATRICifig|1411691.4.peg.2971

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA Translation: CAA25785.1
L10328 Genomic DNA Translation: AAA62080.1
U00096 Genomic DNA Translation: AAC76752.1
AP009048 Genomic DNA Translation: BAE77559.1
V00620 Genomic DNA Translation: CAA23894.1
M18980 Genomic DNA Translation: AAA23836.1
PIRiB65176 XNECGM
RefSeqiNP_418185.1, NC_000913.3
WP_000334099.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JXAX-ray3.10A/B/C2-609[»]
1MOQX-ray1.57A242-609[»]
1MORX-ray1.90A242-609[»]
1MOSX-ray2.00A242-609[»]
1XFFX-ray1.80A/B2-241[»]
1XFGX-ray1.85A/B2-241[»]
2J6HX-ray2.35A/B2-609[»]
2VF4X-ray2.95X2-609[»]
2VF5X-ray2.90X2-609[»]
3OOJX-ray2.50A/B/C/D/E/F/G/H3-609[»]
4AMVX-ray2.05A/B/C1-609[»]
ProteinModelPortaliP17169
SMRiP17169
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262136, 528 interactors
DIPiDIP-9775N
IntActiP17169, 8 interactors
STRINGi316407.85676309

Chemistry databases

BindingDBiP17169
DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid
DB02445 2-Deoxy-2-Amino Glucitol-6-Phosphate
DB02007 alpha-D-glucose 6-phosphate
DB02657 Glucosamine 6-Phosphate
DB03581 Glucose-6-Phosphate
DB02446 Glutamine Hydroxamate

Proteomic databases

EPDiP17169
PaxDbiP17169
PRIDEiP17169

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76752; AAC76752; b3729
BAE77559; BAE77559; BAE77559
GeneIDi948241
KEGGiecj:JW3707
eco:b3729
PATRICifig|1411691.4.peg.2971

Organism-specific databases

EchoBASEiEB0377
EcoGeneiEG10382 glmS

Phylogenomic databases

eggNOGiENOG4105C46 Bacteria
COG0449 LUCA
HOGENOMiHOG000258898
InParanoidiP17169
KOiK00820
PhylomeDBiP17169

Enzyme and pathway databases

BioCyciEcoCyc:L-GLN-FRUCT-6-P-AMINOTRANS-MONOMER
MetaCyc:L-GLN-FRUCT-6-P-AMINOTRANS-MONOMER
BRENDAi2.6.1.16 2026

Miscellaneous databases

EvolutionaryTraceiP17169
PROiPR:P17169

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
Gene3Di3.60.20.10, 1 hit
HAMAPiMF_00164 GlmS, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PANTHERiPTHR10937:SF0 PTHR10937:SF0, 1 hit
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGLMS_ECOLI
AccessioniPrimary (citable) accession number: P17169
Secondary accession number(s): P76745, Q2M847
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 184 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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