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UniProtKB - P17133 (RU17_DROME)
Protein
U1 small nuclear ribonucleoprotein 70 kDa
Gene
snRNP-U1-70K
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Mediates the splicing of pre-mRNA by binding to the stem loop I region of U1-snRNA (PubMed:15611175).
Required during oogenesis for nurse cell chromatin dispersal (PubMed:24244416).
2 PublicationsGO - Molecular functioni
- mRNA binding Source: FlyBase
- RNA binding Source: UniProtKB
- snRNA binding Source: GO_Central
- U1 snRNA binding Source: UniProtKB
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- negative regulation of mRNA splicing, via spliceosome Source: FlyBase
- oogenesis Source: FlyBase
- regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
- regulation of RNA splicing Source: UniProtKB
- spermatogenesis Source: FlyBase
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing |
Enzyme and pathway databases
Reactomei | R-DME-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: U1 small nuclear ribonucleoprotein 70 kDaShort name: U1 snRNP 70 kDa Short name: U1-70K Short name: snRNP70 |
Gene namesi | Name:snRNP-U1-70K Synonyms:snRNP27D, snRNP70K ORF Names:CG8749 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0016978, snRNP-U1-70K |
VEuPathDBi | VectorBase:FBgn0016978 |
Subcellular locationi
Nucleus
- Nucleus speckle By similarity
- nucleoplasm By similarity
Nucleus
- catalytic step 2 spliceosome Source: FlyBase
- nuclear speck Source: UniProtKB
- nucleus Source: FlyBase
- precatalytic spliceosome Source: FlyBase
- spliceosomal complex Source: UniProtKB
- U1 snRNP Source: UniProtKB
- U2-type prespliceosome Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081884 | 1 – 448 | U1 small nuclear ribonucleoprotein 70 kDaAdd BLAST | 448 |
Proteomic databases
PaxDbi | P17133 |
PRIDEi | P17133 |
Expressioni
Developmental stagei
Expressed both maternally and zygotically throughout all development.1 Publication
Gene expression databases
Bgeei | FBgn0016978, Expressed in embryo and 49 other tissues |
ExpressionAtlasi | P17133, baseline and differential |
Genevisiblei | P17133, DM |
Interactioni
Subunit structurei
Component of the U1 snRNP (By similarity).
Interacts with Psi; essential for alternative splicing of P-element transposase.
Interacts with the SMN complex.
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 60131, 25 interactors |
DIPi | DIP-19840N |
IntActi | P17133, 2 interactors |
STRINGi | 7227.FBpp0303792 |
Structurei
3D structure databases
AlphaFoldDBi | P17133 |
BMRBi | P17133 |
SMRi | P17133 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17133 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 102 – 180 | RRMPROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 201 | Required for interaction with U1 RNABy similarityAdd BLAST | 111 | |
Regioni | 188 – 448 | DisorderedSequence analysisAdd BLAST | 261 | |
Regioni | 405 – 425 | Mediates binding to PsiAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 205 – 235 | Basic and acidic residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 249 – 275 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 293 – 381 | Basic and acidic residuesSequence analysisAdd BLAST | 89 | |
Compositional biasi | 391 – 408 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 425 – 448 | Polar residuesSequence analysisAdd BLAST | 24 |
Domaini
Protein interactions mediated by the Arg-rich domain are not essential for viability, but do contribute to an essential U1 snRNP function.1 Publication
The RRM domain mediates interaction with U1 RNA.By similarity
Phylogenomic databases
eggNOGi | KOG0113, Eukaryota |
GeneTreei | ENSGT00940000160292 |
HOGENOMi | CLU_045151_2_1_1 |
InParanoidi | P17133 |
OMAi | GVAKFMA |
OrthoDBi | 1455080at2759 |
PhylomeDBi | P17133 |
Family and domain databases
CDDi | cd12236, RRM_snRNP70, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
IDEALi | IID50033 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034143, snRNP70_RRM IPR022023, U1snRNP70_N |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF12220, U1snRNP70_N, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P17133-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQYLPPNLL ALFAAREPIP FMPPVDKLPH EKKSRGYLGV AKFMADFEDP
60 70 80 90 100
KDTPLPKTVE TRQERLERRR REKAEQVAYK LEREIALWDP TEIKNATEDP
110 120 130 140 150
FRTLFIARIN YDTSESKLRR EFEFYGPIKK IVLIHDQESG KPKGYAFIEY
160 170 180 190 200
EHERDMHAAY KHADGKKIDS KRVLVDVERA RTVKGWLPRR LGGGLGGTRR
210 220 230 240 250
GGNDVNIKHS GREDNERERE RYRLERERED REGPGRGGGS NGLDARPGRG
260 270 280 290 300
FGAERRRSRS RERRDRERDR GRGAVASNGR SRSRSRERRK RRAGSRERYD
310 320 330 340 350
EFDRRDRRDR ERERDRDRER EKKKKRSKSR ERESSRERRE RKRERRDRER
360 370 380 390 400
GTGSGGDVKE RKPDFRDMDV IKIKEEPVDD GYPTFDYQNA TIKREIDDED
410 420 430 440
EEKYRPPPAH HNMFSVPPPP ILGRGNASTN PNPDNGQQSS GDPSWWRQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PC89 | M9PC89_DROME | U1 small nuclear ribonucleoprotein ... | snRNP-U1-70K 70K, Dm70K, Dmel\CG8749, dU1-70K, l(2)02107 | 448 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 278 | N → S in AAA28859 (PubMed:1692955).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31162 Genomic DNA Translation: AAA28859.1 AE014134 Genomic DNA Translation: AAF52471.1 AY061501 mRNA Translation: AAL29049.1 AY089622 mRNA Translation: AAL90360.1 |
PIRi | A36311 |
RefSeqi | NP_001260173.1, NM_001273244.1 NP_477205.1, NM_057857.5 |
Genome annotation databases
EnsemblMetazoai | FBtr0079355; FBpp0078983; FBgn0016978 FBtr0331375; FBpp0303793; FBgn0016978 |
GeneIDi | 33982 |
KEGGi | dme:Dmel_CG8749 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31162 Genomic DNA Translation: AAA28859.1 AE014134 Genomic DNA Translation: AAF52471.1 AY061501 mRNA Translation: AAL29049.1 AY089622 mRNA Translation: AAL90360.1 |
PIRi | A36311 |
RefSeqi | NP_001260173.1, NM_001273244.1 NP_477205.1, NM_057857.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BN5 | NMR | - | B | 405-425 | [»] | |
AlphaFoldDBi | P17133 | |||||
BMRBi | P17133 | |||||
SMRi | P17133 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 60131, 25 interactors |
DIPi | DIP-19840N |
IntActi | P17133, 2 interactors |
STRINGi | 7227.FBpp0303792 |
Proteomic databases
PaxDbi | P17133 |
PRIDEi | P17133 |
Protocols and materials databases
DNASUi | 33982 |
Genome annotation databases
EnsemblMetazoai | FBtr0079355; FBpp0078983; FBgn0016978 FBtr0331375; FBpp0303793; FBgn0016978 |
GeneIDi | 33982 |
KEGGi | dme:Dmel_CG8749 |
Organism-specific databases
CTDi | 33982 |
FlyBasei | FBgn0016978, snRNP-U1-70K |
VEuPathDBi | VectorBase:FBgn0016978 |
Phylogenomic databases
eggNOGi | KOG0113, Eukaryota |
GeneTreei | ENSGT00940000160292 |
HOGENOMi | CLU_045151_2_1_1 |
InParanoidi | P17133 |
OMAi | GVAKFMA |
OrthoDBi | 1455080at2759 |
PhylomeDBi | P17133 |
Enzyme and pathway databases
Reactomei | R-DME-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 33982, 1 hit in 1 CRISPR screen |
EvolutionaryTracei | P17133 |
GenomeRNAii | 33982 |
PROi | PR:P17133 |
Gene expression databases
Bgeei | FBgn0016978, Expressed in embryo and 49 other tissues |
ExpressionAtlasi | P17133, baseline and differential |
Genevisiblei | P17133, DM |
Family and domain databases
CDDi | cd12236, RRM_snRNP70, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
IDEALi | IID50033 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034143, snRNP70_RRM IPR022023, U1snRNP70_N |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF12220, U1snRNP70_N, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RU17_DROME | |
Accessioni | P17133Primary (citable) accession number: P17133 Secondary accession number(s): Q564D5, Q9VM56 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | April 27, 2001 | |
Last modified: | May 25, 2022 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references