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Protein

Oxygen-insensitive NADPH nitroreductase

Gene

nfsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.1 Publication

Cofactori

FMN2 PublicationsNote: Binds 1 FMN per monomer.1 Publication

Kineticsi

  1. KM=11 µM for NADPH1 Publication
  2. KM=5.5 µM for nitrofurazone1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei39FMN; via amide nitrogenCombined sources1 Publication1
    Binding sitei67FMNCombined sources1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 15FMNCombined sources1 Publication5
    Nucleotide bindingi128 – 131FMNCombined sources1 Publication4
    Nucleotide bindingi167 – 169FMNCombined sources1 Publication3

    GO - Molecular functioni

    Keywordsi

    Molecular functionOxidoreductase
    LigandFlavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11261-MONOMER
    MetaCyc:EG11261-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxygen-insensitive NADPH nitroreductaseCurated (EC:1.-.-.-1 Publication)
    Alternative name(s):
    Modulator of drug activity A
    Gene namesi
    Name:nfsA
    Synonyms:mda18, mdaA1 Publication, ybjB
    Ordered Locus Names:b0851, JW0835
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11261 nfsA

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi203R → A: Strong decrease in activity. 1 Publication1
    Mutagenesisi208R → A: No change in activity. 1 Publication1

    Chemistry databases

    DrugBankiDB00698 Nitrofurantoin
    DB03247 Riboflavin Monophosphate

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002055101 – 240Oxygen-insensitive NADPH nitroreductaseAdd BLAST240

    Proteomic databases

    EPDiP17117
    PaxDbiP17117
    PRIDEiP17117

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    holAP286303EBI-1120624,EBI-549153

    Protein-protein interaction databases

    BioGridi4259991, 28 interactors
    DIPiDIP-10334N
    IntActiP17117, 5 interactors
    STRINGi316385.ECDH10B_0921

    Structurei

    Secondary structure

    1240
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP17117
    SMRiP17117
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17117

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin oxidoreductase frp family.Curated

    Phylogenomic databases

    eggNOGiENOG4108DBC Bacteria
    COG0778 LUCA
    HOGENOMiHOG000272869
    InParanoidiP17117
    KOiK10678
    PhylomeDBiP17117

    Family and domain databases

    CDDicd02146 NfsA_FRP, 1 hit
    Gene3Di3.40.109.10, 1 hit
    InterProiView protein in InterPro
    IPR016446 Flavin_OxRdtase_Frp
    IPR029479 Nitroreductase
    IPR000415 Nitroreductase-like
    PANTHERiPTHR43425 PTHR43425, 1 hit
    PfamiView protein in Pfam
    PF00881 Nitroreductase, 1 hit
    PIRSFiPIRSF005426 Frp, 1 hit
    SUPFAMiSSF55469 SSF55469, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P17117-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTPTIELICG HRSIRHFTDE PISEAQREAI INSARATSSS SFLQCSSIIR
    60 70 80 90 100
    ITDKALREEL VTLTGGQKHV AQAAEFWVFC ADFNRHLQIC PDAQLGLAEQ
    110 120 130 140 150
    LLLGVVDTAM MAQNALIAAE SLGLGGVYIG GLRNNIEAVT KLLKLPQHVL
    160 170 180 190 200
    PLFGLCLGWP ADNPDLKPRL PASILVHENS YQPLDKGALA QYDEQLAEYY
    210 220 230 240
    LTRGSNNRRD TWSDHIRRTI IKESRPFILD YLHKQGWATR
    Length:240
    Mass (Da):26,801
    Last modified:February 1, 1995 - v2
    Checksum:i3B389FCDB86DED1D
    GO

    Sequence cautioni

    The sequence CAA33867 differs from that shown. Reason: Frameshift at positions 155, 161 and 187.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18655 Genomic DNA Translation: AAC43450.1
    D38308 Genomic DNA Translation: BAA07425.1
    U00096 Genomic DNA Translation: AAC73938.1
    AP009048 Genomic DNA Translation: BAA35562.1
    X15859 Genomic DNA Translation: CAA33867.1 Frameshift.
    PIRiI80318
    RefSeqiNP_415372.1, NC_000913.3
    WP_000189159.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73938; AAC73938; b0851
    BAA35562; BAA35562; BAA35562
    GeneIDi945483
    KEGGiecj:JW0835
    eco:b0851
    PATRICifig|1411691.4.peg.1427

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18655 Genomic DNA Translation: AAC43450.1
    D38308 Genomic DNA Translation: BAA07425.1
    U00096 Genomic DNA Translation: AAC73938.1
    AP009048 Genomic DNA Translation: BAA35562.1
    X15859 Genomic DNA Translation: CAA33867.1 Frameshift.
    PIRiI80318
    RefSeqiNP_415372.1, NC_000913.3
    WP_000189159.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1F5VX-ray1.70A/B1-240[»]
    ProteinModelPortaliP17117
    SMRiP17117
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259991, 28 interactors
    DIPiDIP-10334N
    IntActiP17117, 5 interactors
    STRINGi316385.ECDH10B_0921

    Chemistry databases

    DrugBankiDB00698 Nitrofurantoin
    DB03247 Riboflavin Monophosphate

    Proteomic databases

    EPDiP17117
    PaxDbiP17117
    PRIDEiP17117

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73938; AAC73938; b0851
    BAA35562; BAA35562; BAA35562
    GeneIDi945483
    KEGGiecj:JW0835
    eco:b0851
    PATRICifig|1411691.4.peg.1427

    Organism-specific databases

    EchoBASEiEB1241
    EcoGeneiEG11261 nfsA

    Phylogenomic databases

    eggNOGiENOG4108DBC Bacteria
    COG0778 LUCA
    HOGENOMiHOG000272869
    InParanoidiP17117
    KOiK10678
    PhylomeDBiP17117

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11261-MONOMER
    MetaCyc:EG11261-MONOMER

    Miscellaneous databases

    EvolutionaryTraceiP17117
    PROiPR:P17117

    Family and domain databases

    CDDicd02146 NfsA_FRP, 1 hit
    Gene3Di3.40.109.10, 1 hit
    InterProiView protein in InterPro
    IPR016446 Flavin_OxRdtase_Frp
    IPR029479 Nitroreductase
    IPR000415 Nitroreductase-like
    PANTHERiPTHR43425 PTHR43425, 1 hit
    PfamiView protein in Pfam
    PF00881 Nitroreductase, 1 hit
    PIRSFiPIRSF005426 Frp, 1 hit
    SUPFAMiSSF55469 SSF55469, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNFSA_ECOLI
    AccessioniPrimary (citable) accession number: P17117
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: February 1, 1995
    Last modified: November 7, 2018
    This is version 144 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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