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UniProtKB - P17117 (NFSA_ECOLI)
Protein
Oxygen-insensitive NADPH nitroreductase
Gene
nfsA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.
1 PublicationCofactori
FMN2 PublicationsNote: Binds 1 FMN per monomer.1 Publication
Kineticsi
- KM=11 µM for NADPH1 Publication
- KM=5.5 µM for nitrofurazone1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | FMN; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 67 | FMNCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 15 | FMNCombined sources1 Publication | 5 | |
Nucleotide bindingi | 128 – 131 | FMNCombined sources1 Publication | 4 | |
Nucleotide bindingi | 167 – 169 | FMNCombined sources1 Publication | 3 |
GO - Molecular functioni
- chromate reductase activity Source: EcoCyc
- FMN binding Source: EcoCyc
- NAD(P)H dehydrogenase (quinone) activity Source: EcoCyc
- oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11261-MONOMER |
BRENDAi | 1.7.1.B3, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Oxygen-insensitive NADPH nitroreductaseCurated (EC:1.-.-.-1 Publication)Alternative name(s): Modulator of drug activity A |
Gene namesi | Name:nfsA Synonyms:mda18, mdaA1 Publication, ybjB Ordered Locus Names:b0851, JW0835 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 203 | R → A: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 208 | R → A: No change in activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB03247, Flavin mononucleotide DB00698, Nitrofurantoin |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205510 | 1 – 240 | Oxygen-insensitive NADPH nitroreductaseAdd BLAST | 240 |
Proteomic databases
jPOSTi | P17117 |
PaxDbi | P17117 |
PRIDEi | P17117 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P17117
With | #Exp. | IntAct |
---|---|---|
holA [P28630] | 3 | EBI-1120624,EBI-549153 |
Protein-protein interaction databases
BioGRIDi | 4259991, 28 interactors 849857, 4 interactors |
DIPi | DIP-10334N |
IntActi | P17117, 5 interactors |
STRINGi | 511145.b0851 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P17117 |
SMRi | P17117 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P17117 |
Family & Domainsi
Sequence similaritiesi
Belongs to the flavin oxidoreductase frp family.Curated
Phylogenomic databases
eggNOGi | COG0778, Bacteria |
HOGENOMi | CLU_070764_0_2_6 |
InParanoidi | P17117 |
OMAi | GMCLGYP |
PhylomeDBi | P17117 |
Family and domain databases
CDDi | cd02146, NfsA-like, 1 hit |
Gene3Di | 3.40.109.10, 1 hit |
InterProi | View protein in InterPro IPR016446, Flavin_OxRdtase_Frp IPR029479, Nitroreductase IPR000415, Nitroreductase-like |
PANTHERi | PTHR43425, PTHR43425, 1 hit |
Pfami | View protein in Pfam PF00881, Nitroreductase, 1 hit |
PIRSFi | PIRSF005426, Frp, 1 hit |
SUPFAMi | SSF55469, SSF55469, 1 hit |
i Sequence
Sequence statusi: Complete.
P17117-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTPTIELICG HRSIRHFTDE PISEAQREAI INSARATSSS SFLQCSSIIR
60 70 80 90 100
ITDKALREEL VTLTGGQKHV AQAAEFWVFC ADFNRHLQIC PDAQLGLAEQ
110 120 130 140 150
LLLGVVDTAM MAQNALIAAE SLGLGGVYIG GLRNNIEAVT KLLKLPQHVL
160 170 180 190 200
PLFGLCLGWP ADNPDLKPRL PASILVHENS YQPLDKGALA QYDEQLAEYY
210 220 230 240
LTRGSNNRRD TWSDHIRRTI IKESRPFILD YLHKQGWATR
Sequence cautioni
The sequence CAA33867 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18655 Genomic DNA Translation: AAC43450.1 D38308 Genomic DNA Translation: BAA07425.1 U00096 Genomic DNA Translation: AAC73938.1 AP009048 Genomic DNA Translation: BAA35562.1 X15859 Genomic DNA Translation: CAA33867.1 Frameshift. |
PIRi | I80318 |
RefSeqi | NP_415372.1, NC_000913.3 WP_000189159.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73938; AAC73938; b0851 BAA35562; BAA35562; BAA35562 |
GeneIDi | 945483 |
KEGGi | ecj:JW0835 eco:b0851 |
PATRICi | fig|1411691.4.peg.1427 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18655 Genomic DNA Translation: AAC43450.1 D38308 Genomic DNA Translation: BAA07425.1 U00096 Genomic DNA Translation: AAC73938.1 AP009048 Genomic DNA Translation: BAA35562.1 X15859 Genomic DNA Translation: CAA33867.1 Frameshift. |
PIRi | I80318 |
RefSeqi | NP_415372.1, NC_000913.3 WP_000189159.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F5V | X-ray | 1.70 | A/B | 1-240 | [»] | |
7NB9 | X-ray | 1.09 | A | 1-240 | [»] | |
7NIY | X-ray | 1.03 | A | 1-240 | [»] | |
7NMP | X-ray | 1.25 | A | 1-240 | [»] | |
7NNX | X-ray | 1.70 | A | 1-240 | [»] | |
AlphaFoldDBi | P17117 | |||||
SMRi | P17117 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259991, 28 interactors 849857, 4 interactors |
DIPi | DIP-10334N |
IntActi | P17117, 5 interactors |
STRINGi | 511145.b0851 |
Chemistry databases
DrugBanki | DB03247, Flavin mononucleotide DB00698, Nitrofurantoin |
Proteomic databases
jPOSTi | P17117 |
PaxDbi | P17117 |
PRIDEi | P17117 |
Genome annotation databases
EnsemblBacteriai | AAC73938; AAC73938; b0851 BAA35562; BAA35562; BAA35562 |
GeneIDi | 945483 |
KEGGi | ecj:JW0835 eco:b0851 |
PATRICi | fig|1411691.4.peg.1427 |
Organism-specific databases
EchoBASEi | EB1241 |
Phylogenomic databases
eggNOGi | COG0778, Bacteria |
HOGENOMi | CLU_070764_0_2_6 |
InParanoidi | P17117 |
OMAi | GMCLGYP |
PhylomeDBi | P17117 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11261-MONOMER |
BRENDAi | 1.7.1.B3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P17117 |
PROi | PR:P17117 |
Family and domain databases
CDDi | cd02146, NfsA-like, 1 hit |
Gene3Di | 3.40.109.10, 1 hit |
InterProi | View protein in InterPro IPR016446, Flavin_OxRdtase_Frp IPR029479, Nitroreductase IPR000415, Nitroreductase-like |
PANTHERi | PTHR43425, PTHR43425, 1 hit |
Pfami | View protein in Pfam PF00881, Nitroreductase, 1 hit |
PIRSFi | PIRSF005426, Frp, 1 hit |
SUPFAMi | SSF55469, SSF55469, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NFSA_ECOLI | |
Accessioni | P17117Primary (citable) accession number: P17117 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families