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Protein

Centromere-binding protein 1

Gene

CBF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for chromosome stability and methionine prototrophy. It is involved in chromosomal segregation. Binds to a highly conserved DNA sequence (5'-RTCACRTG-3'), called CDEI, found in centromeres and in several promoters. DNA-binding activity is enhanced by MET28. Required as an auxiliary factor for transcriptional activation of sulfur metabolism together with MET4 and MET28.2 Publications

Miscellaneous

Present with 6890 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31693-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere-binding protein 1
Short name:
CBP-1
Alternative name(s):
Centromere promoter factor 1
Centromere-binding factor 1
Gene namesi
Name:CBF1
Synonyms:CEP1, CP1, CPF1
Ordered Locus Names:YJR060W
ORF Names:J1730
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR060W
SGDiS000003821 CBF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271611 – 351Centromere-binding protein 1Add BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei45Phosphoserine; by ATM or ATRCombined sources1
Modified residuei48PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Modified residuei138PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP17106
PaxDbiP17106
PRIDEiP17106

PTM databases

iPTMnetiP17106

Interactioni

Subunit structurei

Binds DNA as a dimer. Associates with MET4 to form a heteromeric complex which also includes MET28.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33817, 545 interactors
ComplexPortaliCPX-1016 CBF1-MET4-MET28 sulfur metabolism transcription factor complex
DIPiDIP-2239N
IntActiP17106, 4 interactors
MINTiP17106
STRINGi4932.YJR060W

Structurei

3D structure databases

ProteinModelPortaliP17106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 270bHLHPROSITE-ProRule annotationAdd BLAST49

Phylogenomic databases

HOGENOMiHOG000111343
InParanoidiP17106
KOiK22484
OrthoDBiEOG092C3RR5

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P17106-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNSLANNNKL STEDEEIHSA RKRGYNEEQN YSEARKKQRD QGLLSQESND
60 70 80 90 100
GNIDSALLSE GATLKGTQSQ YESGLTSNKD EKGSDDEDAS VAEAAVAATV
110 120 130 140 150
NYTDLIQGQE DSSDAHTSNQ TNANGEHKDS LNGERAITPS NEGVKPNTSL
160 170 180 190 200
EGMTSSPMES TQQSKNDMLI PLAEHDRGPE HQQDDEDNDD ADIDLKKDIS
210 220 230 240 250
MQPGRRGRKP TTLATTDEWK KQRKDSHKEV ERRRRENINT AINVLSDLLP
260 270 280 290 300
VRESSKAAIL ACAAEYIQKL KETDEANIEK WTLQKLLSEQ NASQLASANE
310 320 330 340 350
KLQEELGNAY KEIEYMKRVL RKEGIEYEDM HTHKKQENER KSTRSDNPHE

A
Length:351
Mass (Da):39,388
Last modified:February 21, 2006 - v2
Checksum:i068F7FA578CB90FC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144V → F in CAA36382 (PubMed:2249662).Curated1
Sequence conflicti168M → T in AAS56245 (PubMed:17322287).Curated1
Sequence conflicti262C → R (PubMed:2185892).Curated1
Sequence conflicti262C → R (PubMed:2249662).Curated1
Sequence conflicti262C → R (PubMed:2188087).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33620 Genomic DNA Translation: AAA34490.1
X52137 Genomic DNA Translation: CAA36382.1
M34070 Genomic DNA Translation: AAA34524.1
L47993 Genomic DNA Translation: AAB39286.1
Z49560 Genomic DNA Translation: CAA89588.1
AY557919 Genomic DNA Translation: AAS56245.1
BK006943 Genomic DNA Translation: DAA08847.1
PIRiS57079 JSBYP1
RefSeqiNP_012594.1, NM_001181718.1

Genome annotation databases

EnsemblFungiiYJR060W; YJR060W; YJR060W
GeneIDi853523
KEGGisce:YJR060W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33620 Genomic DNA Translation: AAA34490.1
X52137 Genomic DNA Translation: CAA36382.1
M34070 Genomic DNA Translation: AAA34524.1
L47993 Genomic DNA Translation: AAB39286.1
Z49560 Genomic DNA Translation: CAA89588.1
AY557919 Genomic DNA Translation: AAS56245.1
BK006943 Genomic DNA Translation: DAA08847.1
PIRiS57079 JSBYP1
RefSeqiNP_012594.1, NM_001181718.1

3D structure databases

ProteinModelPortaliP17106
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33817, 545 interactors
ComplexPortaliCPX-1016 CBF1-MET4-MET28 sulfur metabolism transcription factor complex
DIPiDIP-2239N
IntActiP17106, 4 interactors
MINTiP17106
STRINGi4932.YJR060W

PTM databases

iPTMnetiP17106

Proteomic databases

MaxQBiP17106
PaxDbiP17106
PRIDEiP17106

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR060W; YJR060W; YJR060W
GeneIDi853523
KEGGisce:YJR060W

Organism-specific databases

EuPathDBiFungiDB:YJR060W
SGDiS000003821 CBF1

Phylogenomic databases

HOGENOMiHOG000111343
InParanoidiP17106
KOiK22484
OrthoDBiEOG092C3RR5

Enzyme and pathway databases

BioCyciYEAST:G3O-31693-MONOMER

Miscellaneous databases

PROiPR:P17106

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCBF1_YEAST
AccessioniPrimary (citable) accession number: P17106
Secondary accession number(s): D6VWN1, P17623, Q6Q5N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 21, 2006
Last modified: September 12, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
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Main funding by: National Institutes of Health

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