UniProtKB - P17004 (HEMA_INCKY)
Protein
Hemagglutinin-esterase-fusion glycoprotein
Gene
HE
Organism
Influenza C virus (strain C/Kyoto/41/1982)
Status
Functioni
Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell (By similarity).By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 57 | NucleophileBy similarity | 1 | |
Active sitei | 352 | Charge relay systemBy similarity | 1 | |
Active sitei | 355 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- host cell surface receptor binding Source: InterPro
- sialate O-acetylesterase activity Source: UniProtKB-EC
GO - Biological processi
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- fusion of virus membrane with host plasma membrane Source: InterPro
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)Short name: HEF Cleaved into the following 2 chains: |
Gene namesi | Name:HE |
Organismi | Influenza C virus (strain C/Kyoto/41/1982) |
Taxonomic identifieri | 203233 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Gammainfluenzavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Subcellular locationi
- Virion membrane Curated; Single-pass type I membrane protein Curated
- Host cell membrane By similarity; Single-pass type I membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 616 | ExtracellularSequence analysisAdd BLAST | 616 | |
Transmembranei | 617 – 637 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 638 – 641 | CytoplasmicSequence analysis | 4 |
GO - Cellular componenti
- host cell plasma membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- viral envelope Source: UniProtKB-KW
- virion membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cell membrane, Host membrane, Membrane, Viral envelope protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000039154 | 1 – 432 | Hemagglutinin-esterase-fusion glycoprotein chain 1Add BLAST | 432 | |
ChainiPRO_0000039155 | 433 – 641 | Hemagglutinin-esterase-fusion glycoprotein chain 2Add BLAST | 209 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 6 ↔ 569 | Interchain (between HEF1 and HEF2 chains)By similarity | ||
Glycosylationi | 12 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 47 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Disulfide bondi | 106 ↔ 151 | By similarity | ||
Disulfide bondi | 126 ↔ 174 | By similarity | ||
Glycosylationi | 130 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Disulfide bondi | 196 ↔ 238 | By similarity | ||
Disulfide bondi | 215 ↔ 302 | By similarity | ||
Disulfide bondi | 223 ↔ 275 | By similarity | ||
Disulfide bondi | 332 ↔ 338 | By similarity | ||
Glycosylationi | 381 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 |
Post-translational modificationi
In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Homotrimer of disulfide-linked HEF1-HEF2.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 26 | Fusion domain-1By similarityAdd BLAST | 26 | |
Regioni | 27 – 137 | Esterase domain-1By similarityAdd BLAST | 111 | |
Regioni | 137 – 296 | N-acetyl-9-O-acetylneuraminic acid bindingBy similarityAdd BLAST | 160 | |
Regioni | 297 – 351 | Esterase domain-2By similarityAdd BLAST | 55 | |
Regioni | 352 – 637 | Fusion domain-2By similarityAdd BLAST | 286 |
Sequence similaritiesi
Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR008980, Capsid_hemagglutn IPR007142, Hemagglutn-estrase_core IPR003860, Hemagglutn-estrase_hemagglutn IPR014831, Hemagglutn_stalk_influenz-C |
Pfami | View protein in Pfam PF03996, Hema_esterase, 1 hit PF02710, Hema_HEFG, 1 hit PF08720, Hema_stalk, 1 hit |
SUPFAMi | SSF49818, SSF49818, 1 hit |
i Sequence
Sequence statusi: Fragment.
: The displayed sequence is further processed into a mature form. Sequence processingi
P17004-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
EKIKICLQKQ VNSSFSLHNG FGGNLYATEE KRMFELVKPK AGASVLNQST
60 70 80 90 100
WIGFGDSRTD KSNPNFPRSA DVSVKTANKF RSLTGGSLML SMFGPPGKVD
110 120 130 140 150
YLYQGCGKHK VFYEGVNWSP HAAIDCYRKN WTDIKLNFQK NIYELASQSH
160 170 180 190 200
CMSLVNALDK TIPLQATAGV AGNCNNSFLK NPALYTQKVT PPXXKCGKEN
210 220 230 240 250
LAFFTLPTQF GTYECRLHLV ASCYFIYDSK EVYNKRGCDN YFQVIYDSSG
260 270 280 290 300
KVVGGLDNRV SPYTGNSGDT PTMQCDMIQL KPGRYSVRSS PRFLLMPERS
310 320 330 340 350
YCFDMKEKGP VTAVQSIWGK DRKSDYAVDQ ACLSTPGCML IQKQKPYTGE
360 370 380 390 400
ADDHHGDQEM RELLSGLDYE ARCISQSGWV NETSPFTEEY LLPPKFGRCP
410 420 430 440 450
LAAKEESIPK IPDGLLIPTS GTDTTVTKPK SRIFGIDDLI IGLLFVAIVE
460 470 480 490 500
AGIGGYLLGS RKESGGGVTK ESAEKGFEKI GNDIQILRSS TNIAIEKLND
510 520 530 540 550
RISHDEQAIR DLTLEIENAR SEALLGELGI IRALLVGNIS IGLQESLWEL
560 570 580 590 600
ASEITNRAGD LAVEISPGCW IIDNNICDQS CQNFIFKFNE TAPVPTIPPL
610 620 630 640
DTKIDLQSDP FYWGSSLGLA ITAAISLAAL VISGIAICRT K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25361 Genomic RNA Translation: AAA43789.1 |
PIRi | A32665, HMIVEA |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25361 Genomic RNA Translation: AAA43789.1 |
PIRi | A32665, HMIVEA |
3D structure databases
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR008980, Capsid_hemagglutn IPR007142, Hemagglutn-estrase_core IPR003860, Hemagglutn-estrase_hemagglutn IPR014831, Hemagglutn_stalk_influenz-C |
Pfami | View protein in Pfam PF03996, Hema_esterase, 1 hit PF02710, Hema_HEFG, 1 hit PF08720, Hema_stalk, 1 hit |
SUPFAMi | SSF49818, SSF49818, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEMA_INCKY | |
Accessioni | P17004Primary (citable) accession number: P17004 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | October 7, 2020 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |