Sparc

Functionⁱ

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca²⁺ with a low affinity and an EF-hand loop that binds a Ca²⁺ ion with a high affinity.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Calcium bindingⁱ	272 – 283	By similarityAdd BLAST		12

GO - Molecular functionⁱ

calcium ion binding
Source: RGD
Inferred from direct assayⁱ
- 2322281
collagen binding Source: GO_Central
extracellular matrix binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bone development Source: Ensembl
cellular response to growth factor stimulus Source: Ensembl
heart development
Source: RGD
Inferred from expression patternⁱ
- 8287436
inner ear development
Source: RGD
Inferred from expression patternⁱ
- 11335086
lung development
Source: RGD
Inferred from mutant phenotypeⁱ
- 7654384
negative regulation of angiogenesis Source: Ensembl
negative regulation of endothelial cell proliferation Source: Ensembl
ossification
Source: RGD
Inferred from expression patternⁱ
- 12365801
pigmentation Source: Ensembl
positive regulation of endothelial cell migration Source: Ensembl
regulation of cell morphogenesis Source: Ensembl
response to cadmium ion
Source: RGD
Inferred from expression patternⁱ
- 9232954
response to calcium ion
Source: RGD
Inferred from expression patternⁱ
- 11897705
response to cAMP
Source: RGD
Inferred from expression patternⁱ
- 10703670
response to cytokine
Source: RGD
Inferred from expression patternⁱ
- 10703670
response to ethanol
Source: RGD
Inferred from expression patternⁱ
- 11371715
response to glucocorticoid
Source: RGD
Inferred from expression patternⁱ
- 9232954
response to gravity
Source: RGD
Inferred from expression patternⁱ
- 17384275
response to L-ascorbic acid
Source: RGD
Inferred from expression patternⁱ
- 9495521
response to lead ion
Source: RGD
Inferred from expression patternⁱ
- 1412468
response to lipopolysaccharide
Source: RGD
Inferred from expression patternⁱ
- 10703670
response to peptide hormone
Source: RGD
Inferred from expression patternⁱ
- 15779007
wound healing
Source: RGD
Inferred from expression patternⁱ
- 8245406

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Ligand	Calcium, Copper, Metal-binding

Ligand

Calcium, Copper, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-RNO-114608 Platelet degranulation R-RNO-3000178 ECM proteoglycans R-RNO-3000497 Scavenging by Class H Receptors

Reactomeⁱ

R-RNO-114608 Platelet degranulation
R-RNO-3000178 ECM proteoglycans
R-RNO-3000497 Scavenging by Class H Receptors

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SPARC Alternative name(s): Basement-membrane protein 40 Short name: BM-40 Osteonectin Short name: ON Secreted protein acidic and rich in cysteine
Gene namesⁱ	Name:Sparc
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 10

Organism-specific databases

Rat genome database More...RGDⁱ	3742 Sparc

RGDⁱ

3742 Sparc

Keywords - Cellular componentⁱ

Basement membrane, Extracellular matrix, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 17	1 PublicationAdd BLAST		17
ChainⁱPRO_0000020306	18 – 301	SPARCAdd BLAST		284

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	70 ↔ 81	PROSITE-ProRule annotation
Disulfide bondⁱ	75 ↔ 91	PROSITE-ProRule annotation
Disulfide bondⁱ	93 ↔ 128	PROSITE-ProRule annotation
Disulfide bondⁱ	99 ↔ 121	PROSITE-ProRule annotation
Disulfide bondⁱ	110 ↔ 147	PROSITE-ProRule annotation
Glycosylationⁱ	114	N-linked (GlcNAc...) asparagineCurated	1
Disulfide bondⁱ	153 ↔ 263	PROSITE-ProRule annotation
Disulfide bondⁱ	271 ↔ 287	PROSITE-ProRule annotation

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	P16975
PRIDEⁱ	P16975

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000012840 Expressed in 10 organ(s), highest expression level in lung
Genevisibleⁱ	P16975 RN

Interactionⁱ

Protein-protein interaction databases

IntActⁱ	P16975, 1 interactor
STRINGⁱ	10116.ENSRNOP00000017486

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P16975
SMRⁱ	P16975
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	69 – 91	Follistatin-likeAdd BLAST	23
Domainⁱ	87 – 149	Kazal-likePROSITE-ProRule annotationAdd BLAST	63
Domainⁱ	259 – 294	EF-handAdd BLAST	36

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	23 – 68	Asp/Glu-rich (acidic; binds calcium)Add BLAST		46

Sequence similaritiesⁱ

Belongs to the SPARC family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG4004 Eukaryota ENOG41101WW LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046787
HOGENOMⁱ	HOG000088654
HOVERGENⁱ	HBG002746
InParanoidⁱ	P16975
OMAⁱ	KDFPRRM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0CPG

Family and domain databases

InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR003645 Fol_N IPR015369 Follistatin/Osteonectin_EGF IPR002350 Kazal_dom IPR036058 Kazal_dom_sf IPR001999 Osteonectin_CS IPR037641 SPARC IPR019577 SPARC/Testican_Ca-bd-dom
PANTHERⁱ	PTHR13866 PTHR13866, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09289 FOLN, 1 hit PF00050 Kazal_1, 1 hit PF10591 SPARC_Ca_bdg, 1 hit
SMARTⁱ	View protein in SMART SM00274 FOLN, 1 hit SM00280 KAZAL, 1 hit
SUPFAMⁱ	SSF100895 SSF100895, 1 hit SSF47473 SSF47473, 1 hit
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS51465 KAZAL_2, 1 hit PS00612 OSTEONECTIN_1, 1 hit PS00613 OSTEONECTIN_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P16975-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ TEAAEEMVAE ETVVEETGLP VGANPVQVEM 
        60         70         80         90        100
GEFEEGAEET VEEVVAENPC QNHHCKHGKV CELDESNTPM CVCQDPTSCP 
       110        120        130        140        150
APIGEFEKVC SNDNKTFDSS CHFFATKCTL EGTKKGHKLH LDYIGPCKYI 
       160        170        180        190        200
APCLDSELTE FPLRMRDWLK NVLVTLYERD EGNNLLTEKQ KLRVKKIHEN 
       210        220        230        240        250
EKRLEAGDHP VELLARDFEK NYNMYIFPVH WQFGQLDQHP IDGYLSHTEL 
       260        270        280        290        300
APLRAPLIPM EHCTTRFFET CDLDNDKYIA LEEWAGCFGI KEQDINKDLV 

I

Length:301

Mass (Da):34,296

Last modified:May 1, 2007 - v4

Checksum:ⁱF522EA716C7EF1A1

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	61	V → P in BAA06029 (PubMed:8660135).Curated	1
Sequence conflictⁱ	67	E → CN in BAA06029 (PubMed:8660135).Curated	1
Sequence conflictⁱ	82 – 84	ELD → DG in BAA06029 (PubMed:8660135).Curated	3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D28875 mRNA Translation: BAA06029.1 Y13714 mRNA Translation: CAA74042.1 BC061777 mRNA Translation: AAH61777.1
PIRⁱ	A34572
NCBI Reference Sequences More...RefSeqⁱ	NP_036788.1, NM_012656.1
UniGeneⁱ	Rn.234316 Rn.98989

Genome annotation databases

Ensemblⁱ	ENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840
GeneIDⁱ	24791
KEGGⁱ	rno:24791
UCSC genome browser More...UCSCⁱ	RGD:3742 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P16975	SPARC		PIG		300	UniRef90_P16975
SPARC (Fragment)		RABIT		273
Secreted protein, acidic, cysteine-rich (Osteonectin)		PTEAL		363
SPARC		CAMFR		333
SPARC (Fragment)		MUSPF		308
+146

Protein	Similar proteins		Species	Score	Length	Source
P16975	SPARC		MOUSE		302	UniRef50_P07214
SPARC		PIG		300
SPARC (Fragment)		RABIT		273
SPARC		COTJA		298
SPARC		XENLA		300
+407

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D28875 mRNA Translation: BAA06029.1 Y13714 mRNA Translation: CAA74042.1 BC061777 mRNA Translation: AAH61777.1
PIRⁱ	A34572
RefSeqⁱ	NP_036788.1, NM_012656.1
UniGeneⁱ	Rn.234316 Rn.98989

3D structure databases

ProteinModelPortalⁱ	P16975
SMRⁱ	P16975
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P16975, 1 interactor
STRINGⁱ	10116.ENSRNOP00000017486

Proteomic databases

PaxDbⁱ	P16975
PRIDEⁱ	P16975

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840
GeneIDⁱ	24791
KEGGⁱ	rno:24791
UCSCⁱ	RGD:3742 rat

Organism-specific databases

CTDⁱ	6678
RGDⁱ	3742 Sparc

Phylogenomic databases

eggNOGⁱ	KOG4004 Eukaryota ENOG41101WW LUCA
GeneTreeⁱ	ENSGT00510000046787
HOGENOMⁱ	HOG000088654
HOVERGENⁱ	HBG002746
InParanoidⁱ	P16975
OMAⁱ	KDFPRRM
OrthoDBⁱ	EOG091G0CPG

Enzyme and pathway databases

Reactomeⁱ	R-RNO-114608 Platelet degranulation R-RNO-3000178 ECM proteoglycans R-RNO-3000497 Scavenging by Class H Receptors

Reactomeⁱ

R-RNO-114608 Platelet degranulation
R-RNO-3000178 ECM proteoglycans
R-RNO-3000497 Scavenging by Class H Receptors

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P16975

PROⁱ

PR:P16975

Gene expression databases

Bgeeⁱ	ENSRNOG00000012840 Expressed in 10 organ(s), highest expression level in lung
Genevisibleⁱ	P16975 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR003645 Fol_N IPR015369 Follistatin/Osteonectin_EGF IPR002350 Kazal_dom IPR036058 Kazal_dom_sf IPR001999 Osteonectin_CS IPR037641 SPARC IPR019577 SPARC/Testican_Ca-bd-dom
PANTHERⁱ	PTHR13866 PTHR13866, 1 hit
Pfamⁱ	View protein in Pfam PF09289 FOLN, 1 hit PF00050 Kazal_1, 1 hit PF10591 SPARC_Ca_bdg, 1 hit
SMARTⁱ	View protein in SMART SM00274 FOLN, 1 hit SM00280 KAZAL, 1 hit
SUPFAMⁱ	SSF100895 SSF100895, 1 hit SSF47473 SSF47473, 1 hit
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS51465 KAZAL_2, 1 hit PS00612 OSTEONECTIN_1, 1 hit PS00613 OSTEONECTIN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPRC_RAT
Accessionⁱ	P16975Primary (citable) accession number: P16975 Secondary accession number(s): O08953, Q6GSZ4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
	Last sequence update:	May 1, 2007
	Last modified:	September 12, 2018
	This is version 152 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P16975 (SPRC_RAT)

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Sparc

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ