UniProtKB - P16975 (SPRC_RAT)
Protein
SPARC
Gene
Sparc
Organism
Rattus norvegicus (Rat)
Status
Functioni
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 272 – 283 | By similarityAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: RGD
- collagen binding Source: RGD
- extracellular matrix binding Source: RGD
GO - Biological processi
- anatomical structure development Source: GO_Central
- bone development Source: RGD
- cellular response to growth factor stimulus Source: RGD
- heart development Source: RGD
- inner ear development Source: RGD
- lung development Source: RGD
- negative regulation of angiogenesis Source: RGD
- negative regulation of endothelial cell proliferation Source: RGD
- ossification Source: RGD
- pigmentation Source: RGD
- positive regulation of endothelial cell migration Source: RGD
- regulation of cell morphogenesis Source: RGD
- regulation of cell population proliferation Source: RGD
- regulation of synapse organization Source: SynGO
- response to cadmium ion Source: RGD
- response to calcium ion Source: RGD
- response to cAMP Source: RGD
- response to cytokine Source: RGD
- response to ethanol Source: RGD
- response to glucocorticoid Source: RGD
- response to gravity Source: RGD
- response to L-ascorbic acid Source: RGD
- response to lead ion Source: RGD
- response to lipopolysaccharide Source: RGD
- response to peptide hormone Source: RGD
- wound healing Source: RGD
Keywordsi
Ligand | Calcium, Copper, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-114608, Platelet degranulation R-RNO-3000178, ECM proteoglycans R-RNO-3000497, Scavenging by Class H Receptors |
Names & Taxonomyi
Protein namesi | Recommended name: SPARCAlternative name(s): Basement-membrane protein 40 Short name: BM-40 Osteonectin Short name: ON Secreted protein acidic and rich in cysteine |
Gene namesi | Name:Sparc |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3742, Sparc |
Subcellular locationi
Extracellular region or secreted
- basement membrane 1 Publication
Note: In or around the basement membrane.By similarity
Extracellular region or secreted
- extracellular space Source: RGD
Nucleus
- nuclear matrix Source: RGD
- nucleus Source: RGD
Other locations
- basement membrane Source: UniProtKB-SubCell
- cell surface Source: RGD
- cytoplasm Source: RGD
- extracellular matrix Source: RGD
- glutamatergic synapse Source: SynGO
- platelet alpha granule Source: RGD
- platelet alpha granule membrane Source: RGD
- synapse Source: SynGO
- vesicle Source: RGD
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | 1 PublicationAdd BLAST | 17 | |
ChainiPRO_0000020306 | 18 – 301 | SPARCAdd BLAST | 284 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 70 ↔ 81 | PROSITE-ProRule annotation | ||
Disulfide bondi | 75 ↔ 91 | PROSITE-ProRule annotation | ||
Disulfide bondi | 93 ↔ 128 | PROSITE-ProRule annotation | ||
Disulfide bondi | 99 ↔ 121 | PROSITE-ProRule annotation | ||
Disulfide bondi | 110 ↔ 147 | PROSITE-ProRule annotation | ||
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Disulfide bondi | 153 ↔ 263 | PROSITE-ProRule annotation | ||
Disulfide bondi | 271 ↔ 287 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | P16975 |
PaxDbi | P16975 |
PRIDEi | P16975 |
PTM databases
GlyGeni | P16975, 1 site |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000012840, Expressed in lung and 20 other tissues |
Genevisiblei | P16975, RN |
Interactioni
Protein-protein interaction databases
BioGRIDi | 246915, 1 interactor |
IntActi | P16975, 1 interactor |
STRINGi | 10116.ENSRNOP00000017486 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 69 – 91 | Follistatin-likeAdd BLAST | 23 | |
Domaini | 87 – 149 | Kazal-likePROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 259 – 294 | EF-handAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 23 – 68 | Asp/Glu-rich (acidic; binds calcium)Add BLAST | 46 |
Sequence similaritiesi
Belongs to the SPARC family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4004, Eukaryota |
GeneTreei | ENSGT00510000046787 |
HOGENOMi | CLU_047416_0_0_1 |
InParanoidi | P16975 |
OMAi | LVEWGAC |
OrthoDBi | 1528521at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P16975-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRAWIFFLLC LAGRALAAPQ TEAAEEMVAE ETVVEETGLP VGANPVQVEM
60 70 80 90 100
GEFEEGAEET VEEVVAENPC QNHHCKHGKV CELDESNTPM CVCQDPTSCP
110 120 130 140 150
APIGEFEKVC SNDNKTFDSS CHFFATKCTL EGTKKGHKLH LDYIGPCKYI
160 170 180 190 200
APCLDSELTE FPLRMRDWLK NVLVTLYERD EGNNLLTEKQ KLRVKKIHEN
210 220 230 240 250
EKRLEAGDHP VELLARDFEK NYNMYIFPVH WQFGQLDQHP IDGYLSHTEL
260 270 280 290 300
APLRAPLIPM EHCTTRFFET CDLDNDKYIA LEEWAGCFGI KEQDINKDLV
I
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | V → P in BAA06029 (PubMed:8660135).Curated | 1 | |
Sequence conflicti | 67 | E → CN in BAA06029 (PubMed:8660135).Curated | 1 | |
Sequence conflicti | 82 – 84 | ELD → DG in BAA06029 (PubMed:8660135).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28875 mRNA Translation: BAA06029.1 Y13714 mRNA Translation: CAA74042.1 BC061777 mRNA Translation: AAH61777.1 |
PIRi | A34572 |
RefSeqi | NP_036788.1, NM_012656.1 |
Genome annotation databases
Ensembli | ENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840 |
GeneIDi | 24791 |
KEGGi | rno:24791 |
UCSCi | RGD:3742, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28875 mRNA Translation: BAA06029.1 Y13714 mRNA Translation: CAA74042.1 BC061777 mRNA Translation: AAH61777.1 |
PIRi | A34572 |
RefSeqi | NP_036788.1, NM_012656.1 |
3D structure databases
SMRi | P16975 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246915, 1 interactor |
IntActi | P16975, 1 interactor |
STRINGi | 10116.ENSRNOP00000017486 |
PTM databases
GlyGeni | P16975, 1 site |
Proteomic databases
jPOSTi | P16975 |
PaxDbi | P16975 |
PRIDEi | P16975 |
Genome annotation databases
Ensembli | ENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840 |
GeneIDi | 24791 |
KEGGi | rno:24791 |
UCSCi | RGD:3742, rat |
Organism-specific databases
CTDi | 6678 |
RGDi | 3742, Sparc |
Phylogenomic databases
eggNOGi | KOG4004, Eukaryota |
GeneTreei | ENSGT00510000046787 |
HOGENOMi | CLU_047416_0_0_1 |
InParanoidi | P16975 |
OMAi | LVEWGAC |
OrthoDBi | 1528521at2759 |
Enzyme and pathway databases
Reactomei | R-RNO-114608, Platelet degranulation R-RNO-3000178, ECM proteoglycans R-RNO-3000497, Scavenging by Class H Receptors |
Miscellaneous databases
PROi | PR:P16975 |
Gene expression databases
Bgeei | ENSRNOG00000012840, Expressed in lung and 20 other tissues |
Genevisiblei | P16975, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPRC_RAT | |
Accessioni | P16975Primary (citable) accession number: P16975 Secondary accession number(s): O08953, Q6GSZ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | May 1, 2007 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families